• Clinical and Experimental Pediatrics
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• v.60 no.4
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• pp.106-111
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• 2017

Purpose: This study aimed to determine the prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency among infants with neonatal indirect hyperbilirubinemia (NIH); compare G6PD-deficient and G6PD-normal patients regarding hyperbilirubinemia and need for exchange transfusions (ET); and assess risk factors for ET and kernicterus. Methods: This is a case-control retrospective study. Medical records of NIH patients admitted to the Pediatric Department, Salmaniya Medical Complex, Bahrain, between January 2007 and June 2010 were reviewed. Data on sex, age at presentation, hospitalization duration, need for ET, hemoglobin (Hb) level, reticulocyte count, direct Coombs test, serum total and indirect bilirubin levels, thyroid function, blood and urine cultures, G6PD status, and blood groups were collected and compared between the G6PD-deficent and G6PD-normal patients. Results: Of 1,159 NIH patients admitted, 1,129 were included, of whom 646 (57%) were male. Among 1,046 patients tested, 442 (42%) were G6PD deficient, 49 (4%) needed ET, and 11 (1%) had suspected Kernicterus. The G6PD-deficient patients were mainly male (P<0.0001), and had lower Hb levels (P<0.0001) and higher maximum bilirubin levels (P=0.001). More G6PD-deficient patients needed ET (P<0.0001). G6PD deficiency (P=0.006), lower Hb level (P=0.002), lower hematocrit count (P=0.02), higher bilirubin level (P<0.0001), higher maximal bilirubin level (P<0.0001), and positive blood culture result (P<0.0001) were significant risk factors for ET. Maximal bilirubin level was a significant risk factor for kernicterus (P=0.021) and independently related to ET (P=0.03). Conclusion: G6PD deficiency is an important risk factor for severe NIH. In G6PD-deficent neonates, management of NIH should be hastened to avoid irreversible neurological complications.

• The Journal of the Society of Korean Medicine Diagnostics
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• v.22 no.1
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• pp.57-67
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• 2018

Objectives The objective of this study is to examine the interpretability of the questionnaire-based pattern identification in terms of biosignals. For this purpose, we investigate the relationship between electrochemical skin conductance (ESC) and Qi-Blood-Yin-Yang Deficiency Questionnaire (QBYY-Q) in diabetic patients. Methods A total of 40 patients with diabetes mellitus answered the QBYY-Q and their ESC were measured by SUDOSCAN device (a diabetes screening device, France). To analyze the relationship between QBYY-Q and ESC, ANOVA analysis and Scheffe test were performed and Pearson correlation coefficients were obtained. Results Of the 40 diabetic patients, 23 (57.5%) were males and 17 (42.5%) were females. According to the QBYY-Q, 9 patients were classified into Qi deficiency pattern (QD), 9 patients were Blood deficiency pattern (BD), 10 patients were Yin deficiency pattern (YiD) and 12 patients were Yang deficiency pattern (YaD). Demographic information (age, body mass index, duration of illness, etc.), signs of vitality (blood pressure, body temperature, etc.), fasting plasma glucose and glycated hemoglobin were not significantly different in each deficiency pattern. The ESC of the right leg was significantly lower in the BD group compared to the YiD group (p<0.022). Pearson's correlation coefficient was negatively correlated with the BD questionnaire score (r=-0.343, p <0.05). Finally, ESC showed a positive correlation with hemoglobin and erythrocyte levels in all limbs (r=0.483, p<0.01). Conclusions We showed that ESC could be used to classify the Deficiency pattern identifications in diabetic patients. Especially, the ESC was significantly lower in the BD group and was negatively correlated with the BD scores. It implies the potential utility of the ESC to understand the BD in terms of modern biosignals.

• Journal of Physiology & Pathology in Korean Medicine
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• v.34 no.3
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• pp.142-148
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• 2020

The purpose of this study was to evaluate the validity of Deficiency and Excess Pattern Identification Questionnaire. The number of subjects enrolled in this study was 431. Pearson Correlation Coefficient analysis were conducted to reveal the correlation among Deficiency and Excess Pattern Identification Questionnaire, Grip Strength and Quality of life. ROC-curve analysis were used to suggest optimal cut off value. Cohen Kappa also used to analyze for diagnostic validity. The significant p-value was < .05. The Deficiency questionnaire had positive correlation with the score of the expert and negative correlation with Grip Strength and Quality of life (p<.001). The Excess questionnaire had positive correlation with the score of the expert and negative correlation with Quality of life (p<.001). The optimal cut off value was 56.5 to separate deficiency and non deficiency and 47.5 to separate excess and non-excess. Furthermore, AUC was .900 and .851 accordingly. Cohen Kappa value between deficiency questionnaire and the expert was .640. Cohen Kappa value between excess questionnaire and the expert was .513. This study reveals that Deficiency and Excess Pattern Identification Questionnaire is valid. However, further study considering of gender, age, and health condition and so on, is needed.

• Journal of Nutrition and Health
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• v.25 no.2
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• pp.116-122
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• 1992

This study determined hepatic microsomal lipid peroxide values glucose 6-phosphatase NA-DPH-cytochrome P450 reductase and cytosolic glutathione S-transferase activites to examine the effects of methyl group deficiency on hepatic lipid peroxidation in rats treated with diethylni-trosamine(DEN) and 2-acetylamionfluorene(AAF) Weanling sprague Dawley male rats were fed the diet with methyl group supplemented or deficient. Two weeks after feeding rate were injected with a single of 200mg/kg body weight DEN intraperitoneally and after four weeks 0.02% AAF containing diets were fed for two weeks. Animals were sacrificed at 6th week. Microsomal lipid peroxide values were tended to increase in methyl group deficiency(MD). Especially in case of carcinogen tratments lipid peroxide values were increased significantly in MD. Microsomal glucose 6-phophatase activities were decreased by MD and carcinogens and in MD with carcinogen group (MD+C) the enzyme activites were the lowest Glucose 6-phosphatase activities were negatively correlated with lipid peroxidation. Microsomal NADPH-cytochrome P450 reductase activities were the highest in MD+C and correlated positively with lipid peroxidation. Cytosolic glutathione S-transferase activities were the highest in MD+C Methyl group deficiency induces lipid peroxidation especially in case of being exposed to carcinogens. Therefore the results suggest that lipid peroxidation may be one of the meachanisms of carcinogensis by methyl group deficiency.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.9
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• pp.1326-1335
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• 2005

The main purpose of this study is to investigate the effects of dietary lysine deficiency on protein turnover of porcine muscles. There were 18 LYD three-breed-crossing postweanling barrows from six litters cannulated with gastric tubes through the esophagus at approximate 10 kg of body weight and allocated into three treatment groups. When their body weights reached over 12 kg, one group was sacrificed for determining the initial protein masses of m. masseter, m. longissimus dorsi, m. adductor and m. biceps femoris from the right body side. The others received a diet containing 100% or 61.4% (calculated values) of the lysine requirement (NRC, 1998) multiplied by 1.103 for a period of 17 days. Daily feed provision was computed for each pig according to body weight at the same day. All pigs were infused a flooding dose of $^2$H$_5$-phenylalanine to determine the fractional protein synthesis rates (FSR) of the aforementioned muscles in the end. Their four muscles from the right body side were also dissected for measuring the fractional rates of protein accretion (FAR). As for protein degradation, fractional rates (FDR) were calculated by differences between synthesis and accretion. Results showed that the lysine deficiency resulted in, significantly (p<0.05), lighter body weights, smaller muscles and a slower growth rate. The protein mass, accreted by the muscles, of the deficient group was only 54% averaged of the pigs fed adequately (p<0.05). The FAR of these muscles in the deficient group was significantly lower (p<0.05) and only achieved 61.1% averaged of the control; there was no significant difference (p>0.05), nevertheless, in the amino-acid composition of muscles between two groups. The lysine deficiency reduced significantly (p<0.05) the FSR of m. longissimus dorsi but did not influence its FDR. The m. biceps femoris also presented an inhibited FSR while its FDR reduced only exhibited a very high tendency (p = 0.055) compared to the adequately-fed pigs. As for the m. masseter and m. adductor, both of the FSR and FDR were depressed significantly (p<0.05) by the lysine deficiency, and changes in the FSR were severer than those in the FDR, so that their FAR were significantly slower (p<0.05) in comparison with the control group. The lysine deficiency also inhibited the RNA translation activity of the muscles while the effects on RNA capacity were not significant (p>0.05). In conclusion, the FAR of muscle protein was changed by the current lysine deficiency through the alterations in the FSR and/or FDR.

• Journal of Acupuncture Research
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• v.20 no.1
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• pp.74-84
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• 2003

Objective : We investigated association between excessiveness and deficiency of the visceral and twelve merdians and low back pain, by checking Yangdorak. Methods : Clinical studies were done 62 patients who were treated with low back pain to Dept. of Acupuncture & Moxibustion, Hospital of Oriental Medicine in Semyung University from August 2, 2002 to August 20, 2002. We divided low back pain patients into lumbar vertebra strain, herniated nucleous pulposus(H.N.P.) degenerative spondylosis(D.J.D) and tested the potentiality of skin resistance(Yangdorak) to them. Results: 1. H.N.P. groups were more than another groups in comparing with the States over Physiological Limits and the and excessiveness of merdians. 2. In degenerative spondylosis groups, excessiveness of the F3(kidney) was to be superior. In H.N.P. groups deficiency of the H5(triple energiger) was to be superior. In lumbar vertebra strain groups, deficiency of the H5(triple energiger) and excessiveness of the F2(Liver) was to be very superior. Conclusions: We could investigate the relationship of the excessiveness and deficiency of the visceral and twelve merdians to low back spain patients by checking Yangdorak. Specially, Deficiency of the H5(triple energiger) and H4(Small intestine) may be helpful in diagnosis H.N.P..

• The Journal of Internal Korean Medicine
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• v.35 no.4
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• pp.483-497
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• 2014

Objectives: The aims of study were developing cut-off value of Yin-deficiency questionnaire (YDQ) for diagnosis of Yin-deficiency (YD) and compare diagnostic ability between YDQ and Yin-deficiency scale score (YDS) in xerostomia patients. Methods: We recruited 58 xerostomia patients. They were diagnosed YD or non-YD by 3 Korean medicine doctors (KMD). We assessed YD using YDQ and YDS. We evaluated xerostomia using VAS, Dry Mouth Symptom Questionnaire (DMSQ), Salivary Flow Rate (SFR), oral moisture on buccal mucosa and tongue surface (OMB and OMT). We surveyed tongue coatings using Winkel Tongue Coating Index (WTCI). Results: We diagnosed 23 patients YD and 35 patients non-YD. There were no significant differences of age, sex and body mass index between the YD and non-YD groups. Using receiver operating characteristic curve analysis, the optimal cut-off value of YDQ was defined as 304. Sensitivity, specificity and Youden index of YDQ were 86.96%, 71.43% and 1.5839 respectively. Using Cohen's coefficient of agreement, we found that degree of agreement between KMD and YDQ diagnosis was moderate (${\kappa}$=0.524, p<0.001). Using Pearson's correlation analysis, we found concurrent validity of YDQ and YDS were significant correlated. Using area under curve value, we found diagnostic ability between YDQ and YDS were not significantly different (p=0.505), but there were more strong correlations between DMSQ-symptoms and YDQ (r=0.731, p<0.001) than correlations between DMSQ-symptoms and YDS (r=0.418, p<0.01). Conclusions: The cut-off value of YDQ can diagnose YD in xerostomia and diagnostic ability of YDQ in xerostomia is better than YDS.

• Journal of Genetic Medicine
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• v.20 no.1
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• pp.25-29
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• 2023

The CYP11A1 gene encodes for the cholesterol side-chain cleavage enzyme (P450scc), which initiates steroid hormone biosynthesis. Defective P450scc activity results in severe glucocorticoid and mineralocorticoid deficiencies. We describe a case of P450scc deficiency due to a novel homozygous CYP11A1 variant inherited from the mother with a possibility of uniparental disomy (UPD). The patient was a female, had no family history of endocrine disease, and showed adrenal insufficiency at 13 days of age. Hormonal analysis with an adrenocorticotropic hormone stimulation test showed both glucocorticoid and mineralocorticoid deficiencies, presumed to be a defect of the early stage of steroidogenesis. Exome sequencing reported a novel homozygous frameshift variant of CYP11A1 (c.284_285del, p.Asn95Serfs*10), which was inherited from the mother. Additionally, homozygosity in 15q22.31q26.2, which included CYP11A1, was identified using a chromosomal microarray. It was suggested that the possibility of maternal UPD was involved as the cause of a P450scc deficiency by unmasking the maternally derived affected allele. To our understanding, P450scc deficiency associated with UPD encompassing CYP11A1 had not been reported in Korea before. Genetic analysis can help diagnose rare causes of primary adrenal insufficiency, including P450scc deficiency.

• Journal of Korean Public Health Nursing
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• v.34 no.1
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• pp.74-86
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• 2020

Purpose: This study identified the factors that influenced the food-intake practices affecting iron deficiency anemia among Senegal mothers. Methods: The subjects were 155 mothers in Dakar, Senegal. We surveyed 21 questions concerning their knowledge of iron deficiency anemia, the perceived severity and perceived benefits, self-confidence, food preferences and food-intake practices during the period from December 4, 2017 to December 29, 2017. Results: The factors significantly related to food-intake practices affecting iron deficiency anemia included having a female head of household with male support (β=0.249, p=.003) and being in the 5th quintile of income level (β=0.386, p=.003). Conclusion: The results of regression analysis showed that the head of household and income level are factors that influenced the food-intake practices affecting iron deficiency anemia. Therefore, economic factors and the structure of the household must be considered to achieve success for Senegalese iron deficiency anemia projects.

• Journal of Preventive Medicine and Public Health
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• v.48 no.5
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• pp.249-256
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• 2015

Objectives: Epidemiological studies have reported that vitamin D deficiency is associated with inflammatory disease. Smoking is a well-known risk factor for inflammation. However, few studies have investigated the interactive effect of vitamin D deficiency and smoking on inflammation. This study aims to investigate the interaction of vitamin D and smoking with inflammatory markers in the urban elderly. Methods: We used data from the Korean Elderly Environmental Panel Study, which began in August 2008 and ended in August 2010, and included 560 Koreans ${\geq}60$ years old living in Seoul. Data was collected via questionnaires that included items about smoking status at the first visit. Vitamin D levels, high-sensitivity C-reactive protein (hs-CRP), and white blood cell (WBC) counts were repeatedly measured up to three times. Results: The association of vitamin D and hs-CRP was significant after adjusting for known confounders (${\beta}=-0.080$, p=0.041). After separate analysis by smoking status, the association of vitamin D deficiency and hs-CRP in smokers was stronger than that in nonsmokers (smokers: ${\beta}=-0.375$, p=0.013; non-smokers: ${\beta}=-0.060$, p=0.150). Smoking status was an effect modifier that changed the association between vitamin D deficiency and hs-CRP (interaction estimate: ${\beta}=-0.254$, p=0.032). Vitamin D was not significantly associated with WBC count (${\beta}=0.003$, p=0.805). Conclusions: Vitamin D deficiency was associated with hs-CRP in the urban elderly. Smoking status was an effect modifier of this association. Vitamin D deficiency was not significantly associated with WBC count.