• Radiation Oncology Journal
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• v.29 no.1
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• pp.44-52
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• 2011

Purpose: To evaluate the outcomes and prognostic factors of postoperative radiotherapy (PORT) for patients with pathological stage III non-small-cell lung cancer (NSCLC) at a single institution. Materials and Methods: From 2000 to 2007, 88 patients diagnosed as having pathologic stage III NSCLC after curative resection were treated with PORT. There were 80 patients with pathologic stage IIIA and eight patients with pathologic stage IIIB in the AJCC 6th staging system. The majority of patients (n=83) had pathologic N2 disease, and 56 patients had single station mediastinal LN metastasis. PORT was administered using conventional technique (n=76) or three-dimensional conformal technique (n=12). The median radiation dose was 54 Gy (range, 30.6 to 63 Gy). Thirty-six patients received chemotherapy. Radiation pneumonitis was graded by the Radiation Therapy Oncology Group system, and other treatment-related toxicities were assessed by CTCAE v 3.0. Results: Median survival was 54 months (range, 26 to 77 months). The 5-year overall survival (OS) and disease free survival (DFS) rates were 45% and 38%, respectively. The number of metastatic lymph nodes was associated with overall survival (hazard ratio, 1.037; p-value=0.040). The 5-year locoregional recurrence free survival (LRFS) and distant metastasis free survival (DMFS) rates were 88% and 48%, respectively. Multiple stations of mediastinal lymph node metastasis was associated with decreased DFS and DMFS rates (p-value=0.0014 and 0.0044, respectively). Fifty-one relapses occurred at the following sites: 10 loco-regional, 41 distant metastasis. Grade 2 radiation pneumonitis was seen in three patients, and symptoms were well tolerated with anti-tussive medication. Grade 2 radiation esophagitis was seen in 11 patients. There were no grade 3 or more severe complications associated with PORT. Conclusion: Our retrospective data show that PORT for pathological stage III NSCLC is a safe and feasible treatment and could improve loco-regional control. The number of metastatic lymph nodes and stations of mediastinal lymph node metastasis were analyzed as prognostic factors. Furthermore, efforts are needed to reduce distant metastasis, which is a major failure pattern of advanced stage NSCLC.

• BMB Reports
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• v.40 no.5
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• pp.832-838
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• 2007

A novel inhibitory protein against blood coagulation factor Va (FVa) was purified from muscle protein of granulated ark (Tegillarca granosa, order Arcoida, marine bivalvia) by consecutive FPLC method using anion exchange and gel permeation chromatography. In the results of ESI-QTOF tandem mass analysis and database research, it was revealed that the purified T. granosa anticoagulant protein (TGAP) has 7.7 kDa of molecular mass and its partial sequence, HTHLQRAPHPNALGYHGK, has a high identity (64%) with serine/threonine kinase derived from Rhodopirellula baltica (order Planctomycetales, marine bacteria). TGAP could potently prolong thrombin time (TT), corresponding to inhibition of thrombin (FIIa) formation. Specific factor inhibitory assay showed that TGAP inhibits FVa among the major components of prothrombinase complex. In vitro assay for direct-binding affinity using surface plasmon resonance (SPR) spectrometer indicated that TGAP could be directly bound with FVa. In addition, the binding affinity of FVa to FII was decreased by addition of TGAP in dose-dependant manner ($IC_{50}$ value = 77.9 nM). These results illustrated that TGAP might interact with a heavy chain of FVa ($FVa_H$) bound to FII in prothrombin complex. The present study elucidated that non-cytotoxic T. granosa anticoagulant protein (TGAP) bound to FVa can prolong blood coagulation time by inhibiting conversion of FII to FIIa in blood coagulation cascade. In addition, TGAP did not significantly (P < 0.05) show fibrinolytic activity and cytotoxicity on venous endothelial cell line (ECV 304).

• ALGAE
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• v.18 no.2
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• pp.135-143
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• 2003

The seasonal dynamics of phytoplankton and trophic state were evaluated weekly at three sites in the Lake Unmoon from May to November 2001. The seasonal succession pattern of phytoplankton community in the Lake Unmoon showed that the dominant species were; i) diatoms during the late spring, ii) dinoflagellates in June, iii) blue green algae, diatoms and dinoflagellates in July, iv) green algae and blue green algae in August, v) blue green algae in September and early November, and vi) diatoms in November. Members of Microcystis were dominant from middle August to late October and members of Aulocoseira appeared as important species in autumn in the Lake Unmoon. The concentration of chlorophyll-a ranged from 2.4 to 23.0 mg ${\cdot}m^{-3}$ (average: 8.6 mg ${\cdot}m^{-3}$) during the study period. Concentrations of total phosphorus were high during the period from July to November with the maximum of 0.028 mg ${\cdot} l^{-1}$. The average N/P ratio was 121, indicating that concentrations of phosphorus may determine the high algal biomass in the Lake Unmoon. Concentrations of silicate were higher in the Lake Unmoon (average value: 10.016 mg ${\cdot}$ l-) than in other lakes (average values: 1.074-4.408 mg ${\cdot}$ l-), suggesting high potential of diatom growth. The average trophic state index in the Lake Unmoon was 52, which was close to eutrophic state, and the trophic state trend was increasing steadily since 1999.

• Agricultural and Biosystems Engineering
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• v.3 no.1
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• pp.10-17
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• 2002

Quality attributes of apple are greatly affected by the cooling rate and environmental conditions during storage. Studies were conducted to evaluate the effect of cooling rate on different quality attributes of apple. The effect of $O_2$ pulldown rate of the CA chamber on the quality of apple was also determined. Two methods were used viz. conventional CA procedure and rapid CA procedure. Apples stored by medium and slow cooling methods lost its flesh firmness significantly from an initial level of 4.55 kg to 2.83 kg and 2.27 kg, respectively on 35 days after storage whereas, in rapid cooling, the firmness level changed from 4.55 kg to 3.20 kg on 35 days after storage. At the end of 35 days of storage, titratable acidity decreased insignificantly from an initial value of 0.24l% to 0.239% in the case of rapid CA whereas in the case of conventional CA it dropped significantly to 0.215% from its initial level. The initial flesh firmness of 4.55 kg also changed significantly to 4.05 kg on 35 days after storage in conventional CA whereas in rapid CA it changed to 4.36 kg, which was found to be non-significant at 5% level of significance. Total soluble solids increased from an initial level of $12.43^{\circ}$Bx to $12.60^{\circ}C.$ Bx on 35 days after storage in rapid CA whereas it increased to $13.07^{\circ}$ Bx in conventional CA. Ascorbic acid content of apple juice decreased insignificantly from 6.67 mg/100 mL to 5.87 mg/100 mL on 35 days after storage in rapid CA whereas in conventional CA, it decreased significantly to 5.27 mg/100 mL from its initial level.

• Korean Journal of Environmental Agriculture
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• v.34 no.2
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• pp.139-148
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• 2015

BACKGROUND: The aim of this study was to isolate and identify algicidal bacterium that tends to kill the toxic dinoflagellate Alexandrium catenella, and to determine the algicidal activity and algicidal range of algicide. METHODS AND RESULTS: Among of algicidal bacteria isolated in this study, NH-3 isolate was the strongest algicidal activity against A. catenella. NH-3 isolate was identified on the basis of biochemical characteristics and analysis of 16S rRNA gene sequences. The NH-3 isolate showed over 99% homology with Arthrobacter oxydans, and was designated as Arthrobacter sp. NH-3. The optimal culture conditions were $25^{\circ}C$, initial pH 7.0, and 2.0% (w/v) NaCl concentration. The algicidal activity of Arthrobacter sp. NH-3 was significantly increased to maximum value in the late of logarithmic phase. Arthrobacter sp. NH-3 showed algicidal activity through indirect attack, which excreted active substance into the culture filtrate. When 10% culture filtrate of NH-3 was applied to A. catenella, 100% of algal cells were destroyed within 30 h. In addition, the algicidal activities were increased in dose and time dependent manners. The pure algicide was isolated from the ethyl acetate extract of the culture filtrate of NH-3 by using silica gel column chromatography and high performance liquid chromatography (HPLC). We investigated the algicidal activity of this algicide on the growth of harmful algal bloom (HAB) species, including A. catenella. As a result, it showed algicidal activity against several HAB species at a concentration of $100{\mu}g/mL$ and had a relatively wide host range. CONCLUSION: Taken together, our results suggest that Arthrobacter sp. NH-3 and its algicide could be a candidate for controlling of toxic and harmful algal blooms.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.9
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• pp.1271-1282
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• 2006

The objective of this study was to investigate the effects of either maize or sulla silage supplementation to grazing dairy cows in summer. Forage mixtures used in the four week trial were based on previous experimental results but inclusion of rumen fistulated cows in five treatments enabled rumen sampling and use of in sacco incubations to determine the diet effects on digestion kinetics. Sulla and maize silages were used to supplement pasture and to meet minimum requirements for dietary protein concentration. Five groups of ten cows were grazed on a restricted daily allowance of 18 kg dry matter (DM) pasture/cow to simulate a summer pasture deficit, and four of these five groups received an additional 6 kg DM $cow^{-1}d^{-1}$ of silage (sulla, maize, or sulla and maize silages). A sixth group was given a relatively unrestricted (38 kg DM $cow^{-1}d^{-1}$) pasture allowance. The silage mixtures and pasture were incubated in sacco during the final week of the trial. The pasture was of high nutritive value and not typical of usual summer conditions, which favoured a response to quantity rather than quality of silage supplements. There was no difference in cow performance with the four silage supplements and the low milk solids (MS) production (about 1.0 kg $MS\;d^{-1}$) relative to full pasture (1.3 kg $MS\;d^{-1}$) showed the principal limitation to performance was dry matter intake. Milk composition was not affected by silage type and the low level of pasture substitution (0.29) suggested metabolizable energy (ME) was the principal limitation to performance. Samples of rumen liquor and in sacco data demonstrated significant effects of supplement; DM degradation rates (k) was highest ($0.084h^{-1}$) when cows were fed 6 kg sulla silage whereas diets with a high proportion of maize silage were slowly degraded (p<0.01).

• Journal of Forest and Environmental Science
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• v.36 no.2
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• pp.113-121
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• 2020

This study was carried out in degraded and non-degraded community forests (CF) in the Terai region of Kanchanpur district, Nepal. A total of 63 concentric sample plots each of 500 ㎡ was laid in the inventory for estimating above and below-ground biomass of forests by using systematic random sampling with a sampling intensity of 0.5%. Mallotus philippinensis and Shorea robusta were the most dominant species in degraded and non-degraded CF accounting Importance Value Index (I.V.I) of 97.16 and 178.49, respectively. Above-ground tree biomass carbon in degraded and non-degraded community forests was 74.64±16.34 t ha^-1 and 163.12±20.23 t ha^-1, respectively. Soil carbon sequestration in degraded and non-degraded community forests was 42.55±3.10 t ha^-1 and 54.21±3.59 t ha^-1, respectively. Hence, the estimated total carbon stock was 152.68±22.95 t ha^-1 and 301.08±27.07 t ha^-1 in degraded and non-degraded community forests, respectively. It was found that the carbon sequestration in the non-degraded community forest was 1.97 times higher than in the degraded community forest. CO₂ equivalent in degraded and non-degraded community forests was 553 t ha^-1 and 1105 t ha^-1, respectively. Statistical analysis showed a significant difference between degraded and non-degraded community forests in terms of its total biomass and carbon sequestration potential (p<0.05). Studies indicate that the community forest has huge potential and can reward economic benefits from carbon trading to benefit from the REDD⁺/CDM mechanism by promoting the sustainable conservation of community forests.

• Journal of Electrochemical Science and Technology
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• v.6 no.3
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• pp.106-110
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• 2015

This study investigates Cu(II) complexes of cyclam, propylene cross-bridged cyclam (PCB-cyclam), and propylene cross-bridged cyclam diacetate (PCB-TE2A) as homogeneous electrocatalysts for CO₂ reduction in comparison with Ni(II)-cyclam. It is found that Cu(II)-cyclam can catalyze CO₂ reduction at the potential close to its thermodynamic value (0.75 V vs. Ag/AgCl) in tris-HCl buffer (pH 8.45) on a glassy carbon electrode. Cu(II)-cyclam, however, suffers from severe demetalation due to the insufficient stability of Cu(I)-cyclam. Cu(II)-PCB-cyclam and Cu(II)-PCB-TE2A are revealed to exhibit much less demetalation behavior, but poor CO₂ reduction activities as well. The inferior electrocatalytic ability of Cu(II)-PCB-cyclam is ascribed to its redox potential that is too high for CO₂ reduction, and that of Cu(II)-PCB-TE2A to the steric hindrance preventing facile contact with CO₂ molecules. This study suggests that in addition to the redox potential and chemical stability, the stereochemical aspect has to be considered in designing efficient electrocatalysts for CO₂ reduction.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.26 no.2
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• pp.380-386
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• 2002

Damage behaviors induced in silicon carbide by an impact of particle having different material and size were investigated. Especially, the influence of the impact velocity of particle on the cone crack shape developed was mainly discussed. The damage induced by spherical impact was different depending on the material and size of particles. Ring cracks on the surface of specimen were multiplied by increasing the impact velocity of particle. The steel particle impact produced larger ring cracks than that of SiC particle. In the case of high velocity impact of SiC particle, radial cracks were produced due to the inelastic deformation at the impact site. In the case of the larger particle impact, the damage morphology developed was similar to the case of smaller particle one, but a percussion cone was farmed from the back surface of specimen when the impact velocity exceeded a critical value. The zenithal angle of cone cracks developed into SiC material decreased monotonically with increasing of the particle impact velocity. The size and material of particle influenced more or less on the extent of cone crack shape. An empirical equation, $\theta$= $\theta$$\sub$st/, v$\sub$p/(90-$\theta$$\sub$st/)/500 R$\^$0.3/($\rho$$_1$/$\rho$$_2$)$\^$$\frac{1}{2}$/, was obtained as a function of impact velocity of the particle, based on the quasi-static zenithal angle of cone crack. It is expected that the empirical equation will be helpful to the computational simulation of residual strength in ceramic components damaged by the particle impact.

• Korean Journal of Microbiology
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• v.30 no.2
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• pp.113-118
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• 1992

To elucidate a part of phosphorus cycle an'd the nutritional status of phosphorus. the degradation rates of organic phosphate by phosphatase activity (PA). were studied at the water column of Lake Soyang. from March 1900 to April 1991. Phosphatase activity showed the range of 1-2220 nMillhr. Its maximum value was recortled on August and minimum during October and November. The PA and chlorophyll u v~luess howed high correlation coefficent (0.69). and the values of specific astivity was highest during Winter, 45-12\ulcorner nMihripg chl. a, and lowest on October. 2 nMl hripg chl. 11. The values of PA and bioavailable dissolved inorganic phosphate (DIP) showed negative correlation in surface water. During destratification period (Oct. and Nov. 1990). the values of PA were about 11240 times lower than those during August albeit high concentration of chlorophyll a (1.7- 7.2 mglm'). Such results seem to be cause'd by DIP supply from the metalimnion. hypolimnion ant1 sediment. With these results. phosphate was sufficient to sustain the biomass in Autumn. and internal loading of phosphate shoultl accelerate the eutrophication in Lake Soyang.