• Journal of Microbiology and Biotechnology
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• v.13 no.1
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• pp.139-145
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• 2003

The gene encoding Thermus caldophilus GK24 polyphosphate kinase (Tca PPK) was cloned and sequenced. The gene contains an open reading frame encoding 608 amino acids with a calculated molecular mass of 69,850 Da. The deduced amino acid sequence of Tca PPK showed a 40% homology to Escherichia coli PPK, and $39\%$ to Klebsiella aerogenes PPK. The Tca ppk gene was expressed under the control of the T7lac promoter on pET-22b(+) in E. coli and its enzyme was purified about 70-fold with $36\%$ yield, following heating and HiTrap chelating HP column chromatography. The native enzyme was found to have an approximate molecular mass of 580,000 Da and consisted of eight subunits. The optimum pH and temperature of the enzyme were 5.5 and $70^{\circ}C$, respectively. A divalent cation was required for the enzyme activity, with $Mg^2+$ being the most effective.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.04a
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• pp.68-75
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• 2000

D-Tagatose is a potential bulking agent in food as a non-calorific sweetener. To produce D-tagatose from cheaper resources, plasmids harboring the L-arabinose isomerase gene (araA) from Escherichia coli was constructed because L-arabinose isomerase was previously suggested as an enzyme that mediates the bioconversion of galactose to tagatose as well as that of arabinose to ribulose. In the cultures of recombinant E.coli with pTC101, which harboring araA of E.coli, tagatose was produced from galactose in 9.9 % yield. The enzyme extract of E.coli containing pTC101 also converted galactose into tagatose in 96.4 % yield. For the economic production of D-tagatose, an L-arabinose isomerase of E.coli was immobilized using covalent binding on agarose. While the free L-arabinose isomerase produced tagatose with the rate of 0.48 mg/U$.$day, the immobilized one stably converted galactose into average 7.5 g/l$.$day of tagatose during 7 days with higher productivity of 0.87 mg/U$.$day. In the scaled up immobilized enzyme system, 99.9 g/l of tagatose was produced from galactose with 20 % equilibrium in 48 hrs. The process was stably repeated additional 2 times with tagatose production of 104.1 and 103.5 g/l.

• Journal of Microbiology and Biotechnology
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• v.8 no.4
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• pp.301-304
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• 1998

Methylobacterium organophilum can use nitrogen in the form of ammonium ions ($($NH_4$)_2SO_4\;and\;NH_4Cl) and from nonammonium sources such as glycine, alanine, peptone, and yeast extract. When potassium was limited, significantly more PHB was produced when the ammonium ion was the nitrogen source rather than a nonammonium form. With ammonium, the amount of PHB produced was 0.50-0.53 g PHB/l or $52.0~53.2\%$ of the dry cell weight. If nitrogen was from a nonammonium source, the respective values were 0.04~0.06 g PHB/1 or $8.1~11.3\%$ of dry cell weight. When ammonium sulfate was the sole source of nitrogen under potassium-limited conditions, cell growth and PHB accumulation increased as the pH increased from 6.0 to 7.5. Cell growth and PHB amount at pH 7.5 were 2.50 g dry cell weight/1 and 1.40 g PHB/1, respectively.

• Journal of International Academy of Physical Therapy Research
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• v.6 no.2
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• pp.859-864
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• 2015

The purpose of this study was to investigate the effects of vibration on Golgi tendon organ(GTO) and Hold-Relax of PNF in muscular activity and gait factors on Delayed Onset Muscle Soreness(DOMS). This study was conducted on 20 subjects. they were divided into two groups; Hold-Relax of PNF(n=10), Vibration on GTO(n=10). Both of the group was performed interventions 1 times a day for 3 days. The data was analyzed by the repeated-ANOVA for comparing before, after 24h and after 48h changes of factors in each group and the Independent t-test for comparing the between groups. The results are as follows. There was statistically significant difference of before, after 24h and after 48h vibration on GTO group and Hold-Relax of PNF group in muscular activity and gait factors on DOMS.(p<0.05). There was no statistically significant difference of between vibration on GTO group, but there was statistically significant difference Hold-Relax of PNF group in EMG, step width, step length, stride length(p>0.05). As a results of this study, Hold-Relax of PNF group are effective in improving muscular activity and gait factors.

• Journal of the Korean Chemical Society
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• v.16 no.1
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• pp.18-24
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• 1972

Benzidine dihydrochloride crystallizes in the triclinic system. The space group is $P_1$. The unit cell dimensions are; a = 4.38${\pm}$0.01, b = 5.76${\pm}$0.01, c = 12.82${\pm}$0.02${\AA}$, $\alpha$ = 101.5${\pm}$0.2, $\beta$ = 99.5${\pm}$0.2, $\gamma$ = 99.5${\pm}$0.2$^{\circ}$; with one molecule per unit cell. The crystal structure has been solved by two dimensional Patterson and by trial and error methods, and refined by means of two dimensional differential synthesis. The bond distances are C-C(*) = 1.40${\pm}$0.02, C-C = 1.52${\pm}$0.02, C-N = 1.51${\pm}$0.03 and N-H${\cdot}{\cdot}{\cdot}$Cl = 3.21${\pm}$0.03${\AA}$. The structure consists of hydrogen bonded molecular layers, extending to the (100) plane, and the hydrogen bonding scheme is similar to that of p-phenylenediamine dihydrochloride. The adhesion between hydrogen bonded molecular layers is due to van der Waals forces.

• Korean Journal of Adult Nursing
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• v.20 no.4
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• pp.664-673
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• 2008

Purpose: The purpose of this study was to identify the effect of meridian acupressure on constipation in bed-ridden aged inpatients. Methods: The research design was a non-synchronized non-equivalent control group pretest-posttest design. The subjects consisted of 28 bed-ridden aged inpatients. Meridian acupressure was administered to the experimental group at 30 minutes before breakfast every morning for two weeks. Each meridian was pressured for 10 seconds, 10 times. The frequency of defecation and stool form were examined every day. The data was analyzed by the $x^2$-test, Fisher's exact test, t-test and repeated measures ANOVA. Results: The experimental group given Meridian Acupressure had a higher frequency of defecation(F = 59.372, p = .000) and normal stool(F = 46.046, p = .000) than the control group. Conclusion: The results suggest that Meridian Acupressure was an effective method for the relief of constipation for bed-ridden aged inpatients. A follow -up research is needed to validate that other patients with constipation can use the Meridian Acupressure as a non-invasive nursing intervention instead of a laxative or enema.

• The Journal of Internal Korean Medicine
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• v.1 no.1
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• pp.78-84
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• 1976

The SIB ZUN DAE BO TANG is one of the prevailing tonics appeared at first time on TAE PYUNG HOE MIN HAW ZE GUK BANG in Song’s dynasty and have been widely used up to now. To observe the metabolic effects of Thioacetamide from the SIB ZUN DAE BO TANG, the following experiment was performed. 1. The activation of G.O.T. and G.P.T. to blood serum have been decreased when Thioacetamide was administrated into the rabbit. 2. The coefficiency of the SIB JUN DAE BO TANG appears in 3 hours after administrate and it decreases gradually along with it’s time(1st, 2nd, 3rd day) but the coefficiency of the G.P.T. was more apparent on 2nd, 3rd and 5th day. 3. When administrating Thioacetamide, the total amount of cholesterol grows rapidly up to 3rd day and it continuously grows after the 3rd day. But when administrating SIB ZUN DAE BO TANG, the cholesterol amount in blood grows up to 3rd day, but it decreases gradually after it on the other day. The conclusion of the above experiments shows that the prescription (SIB ZUN DAE BO TANG) improves the metabolic deficiency and the recovery and remedy of diseases.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2004.11a
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• pp.103-106
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• 2004

We have studied a conduction mechanism and equivalent circuit analysis in $Alq_3$ based Organic Light Emitting Diode. The conduction mechanism in organic light emitting diode can be classified into three regions; ohmic region, space-charge-limited current (SCLC) region and trap-charge-limited current (TCLC) region depending on the region of applied voltage. Equivalent circuit model of organic light emitting diode can be established using a parallel combination of resistance $R_p$ and capacitance $C_p$ with a small series resistance $R_s$.

• Korean Journal of Crystallography
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• v.12 no.3
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• pp.145-149
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• 2001

The hydrothermal reaction between zinc(II) nitrate Zn₃(PO₄)₂(H₂O) and potassium phosphate dibasic (K₂HPO₄) in the presence of pyrazine gave a three-dimensional zinc-phosphate coordination polymer with an empirical formula of Zn₃(PO₄)₂(H₂O) (1). Compound 1 was characterized by IR spectroscopy, and X-ray diffraction. Crystallographic data for 1: monoclinic space group. P2₁/c, a=8.970(1)Å, b=4.901(1)Å, c=16.759(3)Å, β=94.98(2)°, Z=4, R(wR₂)=0.0332(0.0927).

• The Korean Journal of Ecology
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• v.25 no.6
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• pp.383-389
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• 2002

Pb and Cd concentrations and reproductive progress of feral pigeons were investigated in urban (Seoul) and industrial complex (Ansan) areas from November 2000 to May 2001. Results of the Pb analysis for the feral pigeons from the Ansan industrial complex (egg contents: 1.13 $\mu$g/wet g, bones of adult: 10.5 $\mu$g/wet g) and Seoul (1.64 $\mu$g/wet g, 29.5 $\mu$g/wet g, respectively) indicated that the Pb level of eggs and bones of adults were significantly different between the two colonies (p<0.05). Cd concentrations in liver and kidney of adult pigeons were also significantly different between the Ansan (liver: 0.14 $\mu\textrm{g}$/wet g, kidney: 0.43 $\mu$g/wet g) and Seoul (liver. 0.24 $\mu$g/wet g, kidney: 1.05 $\mu$g/wet g) colonies. (p<0.05). However, egg size and thickness, incubation period and nestling growth rates did not differ between the study areas. Also, clutch size, number of young hatched per nest and number of young fledglings per nest did not significantly different in the noted areas. Considering the lead and cadmium concentrations of pigeons, these were not as high as those considered as results in toxic effects in other species, and the biological significance from these level differences is uncertain.