• Title/Summary/Keyword: P-FA

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Determination of Loxoprofen Adsorption Isotherms by Frontal Analysis and Pulse Input Method (Frontal Analysis와 Pulse Input Method를 이용한 Loxoprofen의 등온흡착식 결정)

  • Lee, Eun;Park, Joon-Sub;Kim, In-Ho
    • KSBB Journal
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    • v.21 no.5
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    • pp.371-375
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    • 2006
  • Frontal analysis(FA) and Pulsed input method(PIM) have been frequently utilized to measure isotherm of single solute, as well as non-competitive isotherms of two solutes in chromatography(1). FA and PIM were used in this study as complementary methods to measure adsorption isotherms of loxoprofen racemate in HPLC. Prior to FA and PIM experiments, measurements of loxoprofen solubility were made at hexane/ethanol=50/50, 80/20, 95/5(v/v) with acetic acid(0.5%) for adjusting pH. The last composition(95/5) of hexane/ethanol allows us to separate loxoprofen racemate into two forms(retentate, extract). PIM and FA were used to determine the isotherms of re-and ex-loxoprofen.

Detection of E. coli O157:H7 and Listeria monocytogenes, and Appraisal for Microbiological Qualities in the Commercial Frozen Yogurt Products in Korea (국내 시판용 Frozen Yogurt의 병원성 미생물 검출 및 미생물학적 품질 평가에 관한 연구)

  • 윤성식
    • Food Science of Animal Resources
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    • v.18 no.1
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    • pp.63-74
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    • 1998
  • Recently the high outbreaks of intestinal disease caused by the consumption of frozen dairy foods containing pathogenic bacteria has generated considerable interest in the causative agent such as Listeria monocytogenes and E. coli O157:H7. This study was carried out to detect the above pathogens and compare the microbiological qualities of three commercial forzen yogurt products. The main results obtained were as follows. L. monocytogenes coliforms and E. coli O157:H7 were not detected in the total of seven frozen yogurt samples. For microbiological qualities the viable lactic counts of products manufactured by FA company were about 2.9$\times$108 -1.6$\times$109cfu/ml 1.7$\times$106 cfu/ml for FB's and 1.2$\times$106 cfu/ml for FC's The PH values of FA's FB's and FC's products was in the range of pH 4.1~5.3 and the values of FA's were 4.1~4.6 compared by the pH 5.2~5.3 of FB's and FC's products. During refrigeration of the test samples the survival rates of L. monocytogenes spiked into thawed frozen yogurt sample(FA's FB,s and FC's) were 0.55% 15.61% and 16.89% respectively. On the other hand E. coli O157:H7 and L. monocytogenes were 12.4% and 25.0% for FA's 10.8% and 20.8% for FB's and 10.26% and 22.7% for FC's under 37$^{\circ}C$ storage, As the results described above each frozen yogurt products were different in microbiological qualities. The survival rates of pathogens spiked into the samples increased with the pH of the products. This indicates that the pH or any other factors pre-sumable supressed the growth of E. coli O157:H7 and L. monocytogenes in frozen yogurt products.

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Effect of Exposure Concentration and Time of Fuel Additives on the Indigenous Microbial Community in Forests (산림 토착 미생물 군집에 미치는 유류 첨가제 노출 농도 및 시간의 영향)

  • Cho, Won-Sil;Cho, Kyung-Suk
    • Journal of Environmental Health Sciences
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    • v.34 no.5
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    • pp.387-394
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    • 2008
  • The toxicity of methyl tert-butyl ether (MTBE), tert-butyl alcohol (TBA) and formaldehyde (FA) on the indigenous microbial community in forest soil was studied. MTBE, TBA and FA with different concentrations were added into microcosms containing forest soil samples. After 10 and 30 days, total viable cell number and dehydrogenase activity in the microcosms were evaluated. Bacterial communities in the microcosms were also analyzed using a denaturing gradient gel electrophoresis (DGGE). Dehydrogenase activity and total viable cell number were decreased according to the increase of MTBE, TBA and FA concentrations (P<0.05). FA toxicity was the highest, but TBA toxicity was the lowest. The results of principal component analysis using DGGE fingerprints showed that the microbial communities contaminated MTBE, TBA and FA were grouped by exposure time not exposure concentration. Dominant species in the microcosms were as follows: Photobacterium damselae sub sp. and Bacillus sp. KAR28 for MTBE; Mycobacterium sp. and Uncultured Clostridium sp. for TBA; and Uncultured Paenibacillaceae bacterium and Anxynobacillus, Flavithermus for FA.

A Study on the PN code Acquisition for DS/CDMA System over Phase-Error (위상 오류를 고려한 DS/CDMA 시스템의 PN 부호 획득에 관한 연구)

  • 정남모;강찬석;장문기
    • Journal of the Institute of Electronics Engineers of Korea TE
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    • v.39 no.4
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    • pp.403-408
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    • 2002
  • In this paper, the performance on the PN code acquisition of DS/CDMA system was analyzed using the Nakagami-m probability density function considered fading environment. The equations on detection probability, $P_D$ and false alarm probability, ($P_{FA}$, decision variables affecting the PN code acquisition time were derived and proved using simulation in order to analyze the performance. In conclusion, It was necessary increasing the gain of PLL for correcting phase errors and improving the acquisition performance of PN code in apply to the rake receiver.

Flip Angle of the Optimal T1 Effect Using FLASH Pulse Sequence at 3T Abdominal MRI (FLASH를 이용한 3T 복부검사에 있어서 최적의 T1효과를 위한 적정 Flip Angle)

  • Han, Jae-Bok;Choi, Nam-Gil
    • Journal of radiological science and technology
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    • v.32 no.1
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    • pp.101-106
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    • 2009
  • Purpose of this study is to compare the signal intensity (SI) and CNR with T1 weighted image using FLASH at 3T abdominal MRI by varying flip angle (FA). Totally 20 patients (male : 12, female : 8, Age : $28{\sim}63$ years with mean : 51) were examined by 3 Tesla MR scanner (Magnetom Tim Trio, SIEMENS, Germany) with 8 channel body array coil between september and October 2008. Imaging parameters were as follows : FLASH sequence, TR : 120 ms, TE : minimum, FOV (field of view) : $360{\times}300\;mm$, Matrix : $256{\times}224$, slice : 6 mm, scan time : 15 sec and Breath-hold technique. Abdominal image, with a 50 ml syringe filled with water placed in the FOV measuring the water signal, were acquired with varying FA through $10^{\circ}$ to $90^{\circ}$ with $10^{\circ}$ interval. SI's were measured three times at liver parenchyme, water, spleen and background and averaged. The CNR's were measured between the ROIs (region of interest). Statistic analysis was performed with ANOVA test using SPSS software (version 17.0). Less than FA $30^{\circ}$, abdominal images were severely inhomogeneity. Especially, T1 effect of water signal was weak. As the flip angle increased, the signal intensity decreased at all the regions. Especially, flip angle of the highest signal intensity was observed with $40^{\circ}$ at the liver parenchyme, $20^{\circ}$ at water, $30^{\circ}$ at the spleen, respectively. The CNR between liver and water was -60.92 at FA $10^{\circ}$ and 15.16 at FA $80^{\circ}$. The CNR between liver and spleen was -3.18 at FA $10^{\circ}$ and 9.65 at $80^{\circ}$. In conclusion, FA $80^{\circ}$ is optimal for T1 weighted effect using FLASH pulse sequence at 3.0 T abdominal MRI.

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Dietary supplementation of finishing pigs with the docosahexaenoic acid-rich microalgae, Aurantiochytrium limacinum: effects on performance, carcass characteristics and tissue fatty acid profile

  • Moran, Colm A.;Morlacchini, Mauro;Keegan, Jason D.;Fusconi, Giorgio
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.5
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    • pp.712-720
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    • 2018
  • Objective: The aim of this experiment was to evaluate the effect of dietary supplementation with the docosahexaenoic acid (DHA)-rich microalgae, Aurantiochytrium limacinum (AURA) on pig performance, carcass traits, and the fatty acid composition of pork Longissimus lumborum (LL) and backfat. Methods: A total of 144 Pig Improvement Company (PIC)${\times}$Goland finishing pigs (72 females and 72 castrated males) of mean weight 117.1 (${\pm}13.1$) kg were blocked by sex and body weight and provided with 0% or 1% AURA in isonutritive and isocaloric diets. A total of 24 pens provided 12 replicates per treatment. Animals were weighed on day 0 and 28 with feed and water intake recorded per pen. After 31 days supplementation (28 days of study and 3 days until the slaughtering date) three animals per pen (n = 72) were slaughtered and the LL and backfat thickness, lean meat content and dressing percentage were recorded for the carcasses. The fatty acid (FA) profile of the LL and backfat was established by direct FA methyl ester synthesis. Results: No differences were observed for any performance parameters or carcass traits. Supplementation with AURA resulted in significant changes to the FA profiles of both the LL and backfat with male and female pigs responding differently to supplementation in terms of particular FAs. Overall, pork LL samples had significantly higher eicosapentaenoic acid (p<0.001) and DHA concentrations (p<0.001), and higher omega-3 (n-3) FAs (p<0.001), as well as an increased omega3:omega6 (n-3:n-6) ratio (p = 0.001). For backfat, supplementation resulted in significantly higher amounts of DHA (p<0.001) and n-3 FAs (p<0.001). Conclusion: These results indicate that dietary supplementation with 1% AURA over a 31 day period can increase the FA composition of pork LL and backfat, specifically the DHA, with no major impact on growth performance and carcass traits.

Effects of formic acid and lactic acid bacteria inoculant on main summer crop silages in Korea

  • Wei, Sheng Nan;Li, Yan Fen;Jeong, Eun Chan;Kim, Hak Jin;Kim, Jong Geun
    • Journal of Animal Science and Technology
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    • v.63 no.1
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    • pp.91-103
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    • 2021
  • To improve the fermentation quality of silage and reduce the nutrients loss of raw materials during the ensiling process, silage additives are widely used. The effect of additives on silage is also affected by the species of crop. Therefore, this study was designed to explore the effects of formic acid (FA) and lactic acid bacterial inoculant on the quality of main summer crop silage. The experiment was consisted on split-plot design with three replications. The experiment used the main summer forage crops of proso millet ("Geumsilchal"), silage corn ("Gwangpyeongok"), and a sorghum-sudangrass hybrid ("Turbo-gold"). Treatments included silage with Lactic acid bacterial Inoculant (Lactobacillus plantarum [LP], 1.0 × 106 CFU/g fresh matter), with FA (98%, 5 mL/kg), and a control (C, without additive). All silages were stored for 60 days after preparation. All additives significantly increased the crude protein content and in vitro dry matter digestibility (IVDMD) of the silages and also reduced the content of ammonia nitrogen (NH3-N) and pH. Corn had the highest content of IVDMD, total digestible nutrients and relative feed value among silages. Compared with the control, irrespective of whether FA or LP was added, the water soluble carbohydrate (WSC) of three crops was largely preserved and the WSC content in the proso millet treated with FA was the highest. The treatment of LP significantly increased the lactic acid content of the all silage, while the use of FA significantly increased the content of acetic acid (p < 0.05). The highest count of lactic acid bacteria (LAB) was detected in the LP treatment of corn. In all FA treatment groups, the total microorganism and mold numbers were significantly lower than those of the control and LP groups (p < 0.05). In conclusion, both additives improved the fermentation quality and nutritional composition of the main summer forage crops. The application of FA effectively inhibited the fermentation of the three crops, whereas LAB promoted fermentation. So, both FA and LP can improve the quality of various species of silage.

Effects of Different Additives on Fermentation Characteristics and Protein Degradation of Green Tea Grounds Silage

  • Wang, R.R.;Wang, H.L.;Liu, X.;Xu, C.C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.5
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    • pp.616-622
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    • 2011
  • This study evaluated the fermentation characteristics and protein degradation dynamics of wet green tea grounds (WGTG) silage. The WGTG was ensiled with distilled water (control), or lactic acid bacteria (LAB), enzyme (E), formic acid (FA) and formaldehyde (FD) prior to ensiling. Three bag silos for each treatment were randomly opened at 0, 3, 7, 14, 28 and 60 days after anaerobic storage. For all the treatments, except for FA, there was a rapid decline in pH during the first 7 days of ensiling. LAB treatment had higher lactic acid content, lower ammonia-N ($NH_3$-N) and free-amino nitrogen (FAA-N) contents than other treatments (p<0.05). E treatment had higher lactic acid, water-soluble carbohydrates (WSC) and non-protein nitrogen (NPN) content than the control (p<0.05). FA treatment had higher $NH_3$-N and FAA-N content than the control (p<0.05). FD treatment had lower NPN and FAA-N content than the control, but it did not significantly inhibit the protein degradation when compared to LAB treatment (p>0.05). Results indicate that LAB treatment had the best effect on the fermentation characteristics and protein degradation of WGTG silage.

A STATISTICAL STUDY OF CLINICAL CROWN INCLINATION IN KOREAN'S NATURALLY OCCURRING OPTIMAL OCCLUSION (한국인 정상교합자의 치관경사도에 관한 임상통계학적 연구)

  • Kim, Jong-Sung;Jin, Keun-Ho;Hong, Sung-Joon
    • The korean journal of orthodontics
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    • v.22 no.3 s.38
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    • pp.715-733
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    • 1992
  • The objective of this study was to evaluate some clinical aspect of the crown inclination in Korean's naturally occuring optimal occlusion, and to statistically compare the crown inclination at Andrews' FA points group and another bracket slot level group based on marginal ridges. The materials consisted of study models of 30 patients (14; Korean's males, 16; Korean's females) who have nonorthodontic normal occlusion. The results were as follows; 1. Mean, standard deviation, range of clinical inclination in the Andrews' FA points and another bracket slot level were obtained. 2. Statistically difference between Andrews' FA points group and bracket slot level group based on marginal ridge was non significant (p > 0.05). But standard deviation and range in FA points group was more stability than marginal ridge group. 3. A lingual crown inclination at FA points in the upper posterior teeth existed (canines through molars). 4. The lingual crown inclination at FA points in the lower dentition progressively increased from the incisors through the second molars.

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Association of FASN and SCD genes with fatty acid composition in broilers

  • Maharani, Dyah;Seo, Dong-Won;Choi, Nu-Ri;Jin, Shil;Cahyadi, Muhammad;Jo, Cheorun;Lee, Jun-Heon
    • Korean Journal of Agricultural Science
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    • v.40 no.3
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    • pp.215-220
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    • 2013
  • Fatty acids (FAs) were considered in activating nuclear hormone receptors that play significant roles in the cellular lipid metabolism by the regulation of several genes. Previously, fatty acid synthase (FASN) and stearoyl-CoA desaturase (SCD) genes have been known to regulating the FA metabolism. In this study, associations of FASN and SCD genes with fatty acid (FA) composition in broilers were investigated. Tissue samples from 95 Cobb 500 broilers were used for DNA extraction. The g.1222 A>G SNP located in intron 42 of FASN gene and 2 SNPs in SCD gene, one in exon 2 (g.3728A>G) and the other in exon 4 (g.12903G>A), were subjected for genotyping using PCR-RFLP method. One of the SNPs in SCD gene, SNP g.3728A>G had significant association with myristoleic acid (C14:1; P<0.05), palmitic acid (C16:0; P<0.05), palmitoleic acid (C16:1; P<0.05) and saturated FA (SFA; P<0.05). However, the SNP g.1222A>G in FASN gene had only suggestive association with arachidic acid (C20:0; P=0.08). The findings in this study suggest that the SNP in exon 2 of SCD gene can be used as a molecular marker for selecting birds having desirable FA composition in broilers.