• Economic and Environmental Geology
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• v.35 no.2
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• pp.113-120
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• 2002

Oxygen and hydrogen isotopic compositions of stream water in the Han river basin are expressed by the equation of $\delta$D=6.6$\delta$$^{18}$ O-7.4, which is not satisfy the meteoric water line ($\delta$D=8$\delta$$^{18}$ O+10). It might be depended on the local climatic condition and the evaporation effect in the Han river basin. The $\delta$$^{18}$ O and $\delta$D values of stream water in the Han river basin range from -8.2 to -10$\textperthousand$ (avg. -9.1$\textperthousand$) and -60 to -96$\textperthousand$ (avg. -69$\textperthousand$), respectively. The stream water from the South Han river (8$\delta$$^{18}$ O= -8.9~ -10$\textperthousand$, avg.-9.3$\textperthousand$ $\delta$D: -66~ -96$\textperthousand$, avg.-69$\textperthousand$) is slightly more depleted in $^{18}$ O and D than those of North Han river ($\textperthousand$$^{18}$ O= -8.4~ -9.7$\textperthousand$, avg. -9.2$\textperthousand$, $\delta$D= -64~ -95$\textperthousand$, avg. -69$\textperthousand$). It reflects more altitude effect than the effect of latitude and Inflow of the $^{18}$ O eniched S $O_4$$^{2-}$ and HC $O_3$- from the carbonate rock and sulfide minerals in the Taebagsan and Hwanggangri mineralized zone. The Main stream water of the Han river having $\delta$D: -60~ -76$\textperthousand$ (avg.-68$\textperthousand$) and $\textperthousand$$^{18}$ O= -8.2~-10$\textperthousand$ (avg.9.0$\textperthousand$) is enriched in $^{18}$ O compared to the South and North Han river waters, which is caused by the evaporation effect. Binary simple mixing ratio of the Main Han river water between South and North Han river waters was obtained to be 6 : 4 by the isotopic data, suggesting a strong influence of South Han river water to the Main Han river water.

• Journal of Animal Science and Technology
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• v.46 no.6
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• pp.917-924
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• 2004

Using PCR-RFLP haplotyping for the mitochondrial DNA(mtDNA) fragment containing the NADH dehydrogenase 2 gene(ND2) and three tRNA genes(tRNA-Met, tRNA-Trp and tRNA-Ala), we characterized the genetic diversity of five pig breeds including Jeju native pigs. mtDNA polymorphisms showing distinct cleavage patterns were found in the pig breeds. Two digestion patterns were detected when HaeIII- and Hinfl-RFLP, and four in the Tsp5091-RFLP analyses. Combining the three restriction enzyme digestion patterns found in five different pig breeds, four mtDNA haplotypes were observed and the haplotype frequencies were significantly different by the pig breeds. A monomorphic haplotype, mtWB, was observed in both Korean wild boars and Large White pigs. Both Duroc and Landrace pigs contained two haplotypes suggesting their multiple maternal lineages. Jeju native pig has two haplotypes(mtJN and mtJD). Of these, mtJN is identified as a Jeju native pig specific haplotype. This study suggested that more than two progenitor populations have been taken part in the domestication process of the Jeju native pig population, and/or probably subsequent crossing with other pig breeds from near east Asia. Unlike with our prediction, there was no direct evidence under molecular levels on the maternal introgression of Korean wild boar in the domestication of Jeju native pigs. In conclusion, specificity of mtDNA haplotypes related to pig breeds win be useful for identifying the maternal lineage as wen as constructing the genealogical pedigree in pigs.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.3
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• pp.259-264
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• 1999

A field experiment was carried out to determine the optimum application level of dicamba herbicide for efficient control of Calystegia japonica in silage corn field. Six treatments of application levels (0 ; control, $0.75{\ell},\;1.0{\ell},\;1.25{\ell},\;1.5{\ell},\;and\;2.0{\ell}/ha$) were arranged at National Livestock Research Institute, RDA, Suwon in 1995. The hybrid silage corn was DK 729, and dicamba herbicide was applied at 5~6 leaf stage of corn. The growth of corn was poor in control plot, and the days for tasseling and silking were delayed 1~4 days when compared with the plots of herbicide application. The control efficiency of Calystegia japonica was excellent as 74.3~94.6% in the plots of dicamba application. A slight injury of herbicide was observed at early stage of corn when applied at $1.25{\ell}$ and $1.5{\ell}$ of dicamba per ha, and severe injury was found at $2.0{\ell}$ of dicamba. Forage dry matter(DM) yield, and length of ear in the plots of dicamba treatments were significantly higher than those of control. The DM yield of control(4,866kg/ha) was only about 30% of dicamba treatment plot (14,960~16,340kg/ha). However, there was no yield difference among dicamba application level. The percentage of ear to total DM yield was ranged from 33.6 to 39.4%. With application of herbicide, the contents of crude protein and nitrogen free extract were increased, and that of crude fiber was decreased in stover. However, nutritive value of corn ear was very similar among all treatments, regardless of herbicide treatment. From the above results, it is recommended that optimum application level of dicamba herbicide was $0.75{\sim}1.0{\ell}/ha$ for efficient control of Calystegia japonica and for silage corn production without herbicide injury.

• Korean Journal of Ecology and Environment
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• v.43 no.2
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• pp.307-318
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• 2010

This study was to analyze the ecological conditions, based on physical habitat, chemical, and biological conditions before (2006, 2007) and after ecological restoration (2009) in five sites of Changwon Stream (CS) and six sites of Nam Stream (NS), respectively, and then to compare ecological health between the two period. The analysis of ecological health was based on the multimetric models of Index of Biological Integrity (IBI) and Qualitative Habitat Evaluation Index (QHEI) along with water chemistry in the streams. For the study, the models of IBI and QHEI were modified as 8 and 11 metric attributes, respectively. For the evaluations, the survey was conducted in the period of 2006~2007 before the restoration and in 2009 after the restoration by the city. Chemical conditions, based on conductivity, in both streams showed a typical longitudinal declines along the axis of the upstream-to-downstream. There were no significant differences (p>0.05) in water quality between the two periods. Values of IBI in the CS and NS averaged 21.6 and 19.7, respectively, indicating a C grade in the criteria of Ministry of Environment, Korea, and there was no significant differences in the IBI between the two periods. Values of QHEI after the restoration averaged 29.2 and 63.2 in the CS and NS, respectively and the values decreased markedly especially, in the NS (35.3) after the restoration. The habitat disturbance was mainly attributed to destructions (i.e., the narrower width of riparian vegetation and higher substrate exposure by the air) of artificial materials by massive flood in 2009. Overall, our results suggest that the restoration was not effective in the two streams between the two periods, even if the budget was used a lot and that such ecological restoration, not considered the natural disaster, may not effect for the stream restoration.

• Korean Journal of Food Science and Technology
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• v.27 no.6
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• pp.909-914
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• 1995

In order to investigate gelation properties of Gelation Properties of ${\alpha}-lactalbumin$(${\alpha}-La$), gelling times and protein solubilities of ${\alpha}-La$ gels prepared in 0.1 M Tris-HCI buffer(pH 8.0) followed by heating at $90^{\circ}C$ for 40 minutes under different ${\alpha}-La$ concentration, NaCl, $CaCl_2$, N-ethylmaleimide(NEM), and dithiothreitol(DTT) concentration were measured. Gelling times decreased with increasing concentration of ${\alpha}-La$, NaCl, $CaCl_2$, and DTT, but increased with increasing concentration of NEM. ${\alpha}-La$ solutions made from all treatments were gelled within 40 minutes with the exception of NEM at $20{\sim}50\;mM$. Solubilities decreased with increasing concentration of ${\alpha}-La$, NaCl, $CaCl_2$, and DTT, but the solubility of NEM-modified gels increased with increasing concentration of NEM. As the results, solubilities In standard buffer were $10.4{\sim}51.3%$, $9.2{\sim}35.4%$, $11.1{\sim}35.0%$, $8.0{\sim}9.5%$, and $96.8{\sim}56.2%$, respectively. Solubilities in standard buffer containing 8 M urea and 0.5% SDS were higher than those in standard buffer, and were $41.8{\sim}81.3%$, $41.9{\sim}64.1%$, $43.5{\sim}69.8%$, $29.6{\sim}38.5%$, and $77.4{\sim}98.9%$, respectively. Solubilities in the presence of DTT were almost close to 100% in all conditions. These results indicates that the gelation rate and solubility are influenced by many factors, i.e. protein concentration, kind and concentration of salts, concentration of thiol reagents. The solubility of gel decreased with increasing the gelation rate.

• Advances in pediatric surgery
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• v.6 no.2
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• pp.106-123
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• 2000

The purpose of the survey was to clarify the clinical features and management of the anorectal malformations (ARM) in Korea. Twenty-seven members of the Korean Association of Pediatric Surgeons were surveyed. The members completed a case registration form for each patient during the two years period of 1996-1997, and a questionnaire that contained their management preferences for primary and complicated patients. Twenty-four members of the 22 institutions registered 295 cases of the ARM, and 27 out of 34 members (77.1 %) responded to the questionnaire. The patients were classified by the Wingspread classification of ARM(1984). The case registration form was a modification of Wingspread workshop for the postoperative assessment and case registration. The male to female ratio was 1.7 : 1. The average number of patients per surgeon was 6.1 cases per year. Prematurity(< 36 weeks) was present in 1.9 % of cases and low birth weight (< 2.6 Kg) in 12.1 %. Among 187 male patients, 62 were high(H) type, 29 intermediate(l) type, 88 low(L), and 8 unclassified. In female, there were 2 cases of cloacal anomalies(C), 4 H type, 30 I type, 66 L type and 6 unclass ified. For male, 87.9 % of H and I type were operated by the Pena procedure, but only 7.9 % in L types. In female, all of C, H and I types, and 40.9 % of L type were done by the Pena procedure. One case expired (MR; 0.003%) as a result of surgical complication. Over-all complication rate was 12.5 %. Among 6 cases of reoperation, 4 were failed Pena procedures. Among 140 colostomies sigmoid colon was utilized in 75.7%, and loop colostomy was commonly used. Ten surgeons prefered primary maturation of the stoma. In conclusion, posterior sagittal anorectoplasty is popular for high types of ARM in this country, but considerable number of patients are still suffering from failed operations. For better understanding and analysis, necessity of prospective study by new classification was discussed.

• Journal of Aquaculture
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• v.11 no.4
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• pp.573-580
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• 1998

Red spotted grouper, Epinephelus akaara is distributed in the south and west coasts of Korea. The natural stocks of the fish are decreasing sharply year by uear because of reckless overfishing. This research was carried out to understand general informations on maturation, sex composition and sex-reversals of the fish. Annual fishing uields of red spotted grouper in the castal area of Byonsan Peninsular of Kora decreased over 10% from 1992 to 1994. The main fishing season was from May to July with fishing gear of Hand-lines. Gonadosomatic index (GSI) and condition factor were highest on early and late July, respectively, thus main spawning reriod was assumed from late July to early August. The relationship between total length (X) and body weight (Y) for wild adults was represented as a regression, Y=$0.0169X^{2.9705}$, ($r^2$=0.96). Frequency of sex of wild red spotted gouper showed that the number of female below 38cm in total length was more than that of male, and hermaphrodite mainly occurred from 28cm to 32cm in total length the frequency of male and female were almost same. Also hermaphrodite occurred mainly between 25~29cm. Sex reversal ration of the adults reared in a tank for a year with different sexual compositions revealted that the frequency of female reversed from male was more than that of male reversed from female at 1:1 and 1:2 stocking densities of female and male, respectively. Also, about 20% of female was reversed to male when all females were reared. And the size of the fish reversed to male was larger than that of non-reversed female.

• Tuberculosis and Respiratory Diseases
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• v.48 no.4
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• pp.471-477
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• 2000

Background : Coughing is the most common complaint for which patients seek medical service. When caughing continues over 3 weeks in non-smokers who do not take cough-provoking drugs, they are classified as patients with chronic cough. Three well known main causes of chronic caugh are postnasal drip syndrome, bronchial asthma and gastroesophaseal reflux disease. Among them, postnasal drip syndrome is reported to be the most common cause of all in chronic cough diseases, and allergic inflammation plays an important role in the pathogenesis of postnasal drip syndrome. CD23 and CD25 which are low affinity receptor for IgE and IL-2 receptor alpha, respectively, are closely related to allergic inflammation and their roles were evaluated in chronic cough patients. Methods : We evaluated 105 patients with chronic cough and selected 56 patients for measurement of serum CD23 & CD25 levels. We selected 10 normal, medical students for comparison of serum CD23 & CD25 levels. Result : The postnasal drip syndrome was found to be the most common cause of chronic cough. Serum CD23 and CD25 did not increase in chronic cough patient compared to normal controls. However in bronchial asthma patient, serum CD23 level was increased relative to normal control (p<0.05). Conclusion : In bronchial asthma presented as chronic cough, lymphocyte mediated allergic inflammation may related with the pathogenesis of the disease.

• Progress in Medical Physics
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• v.13 no.4
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• pp.209-217
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• 2002

There have been many studies on the application of the reciprocal advantages of multimodality image to define accurate target volume in the Process of radiation treatment planning. For the proper use of the multimodality images, the registration works between different modality images should be performed in advance. In this study, we selected chamfer matching method and mutual information method as most popular methods in recent image registration studies considering the registration accuracy and clinical practicality. And the two registration methods were analyzed to deduce the optimal registration method according to the characteristics of images. Lung phantom of which multimodality images could be acquired was fabricated and CT, MRI and SPECT images of the phantom were used in this study. We developed the registration program which can perform the two registration methods properly and analyzed the registration results which were produced by the developed program in many different images' conditions. Although the overall accuracy of the registration in both chamfer matching method and mutual information method was acceptable, the registration errors in SPECT images which had lower resolution and in degraded images of which data were removed in some part were increased when chamfer matching method was applied. Especially in the case of degraded reference image, chamfer matching methods produce relatively large errors compared with mutual information method. Mutual information method can be estimated as more robust registration method than chamfer matching method in this study because it did not need the prerequisite works, the extraction of accurate contour points, and it produced more accurate registration results consistently regardless of the images' characteristics. The analysis of the registration methods in this study can be expected to provide useful information to the utilization of multimodality images in delineating target volume for radiation treatment planning and in many other clinical applications.

• Clinical and Experimental Reproductive Medicine
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• v.23 no.3
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• pp.319-326
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• 1996

The objective of this study was to investigate correlation between the morphology by microscopic assessments of surplus blastocysts produced in human IVF program and their cell number obtained by differential labelling method. For these experiments, 76 surplus human blastocysts were obtained from 36 patients on day 5 after IVF, the embryos were classified to early (ErB), early expanding (EEB), middle expanding (MEB), expanded blastocyst (EdB) according to their blastocoel expansion and zona thickness. When the ovum size and zona thickness of the classified blastocysts were measured using micrometer, although the embryos were produced in the same culture condition, there were significant variances in ovum size ($148.8 217.6{\mu}m$) and zona thickness ($1.2-14.4{\mu}m$). Total blastomere cell number counted after hoechst staining was increased by two to three fold during the transition period from ErB ($39.1{\pm}3.6$) to EdB ($(89.6{\pm}3.3)$) stage on day 5 after IVF. ICM ($11.9{\pm}1.8-22.2{\pm}4.3$) and TE ($24.5{\pm}3.6-70.0{\pm}7.7$) cell numbers using differential labelling were also showed the increased pattern according to the developmental level. Especially, EdB which showed poor ICM morphologically also indicated the low ICM cell number after differential labelling. This demonstrated that there is good correlation between the morphological assessment and the cell number. The count of ICM and TE nuclei using differential labelling can be used as an important criterion, if it is accompanied with morphological assessments, in selecting the better embryos for improving the pregnancy rates in human blastocyst transfer program.