• Journal of Life Science
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• v.15 no.3 s.70
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• pp.352-358
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• 2005

The purpose of this study was to examine the effect of various exercise intensity on Resting Metabolic Rate (RMR), excess post exercise energy expenditure (EPEE), and thyroid hormonal changes in trained (TR) and untrained (UT) people. The subject of the present study were divided into two groups and four periods: trained (TR; n=6) and untrained (UT; n=6) group. And the periods were divided as follows; Resting (R), Maximal (M), High intensity (H), and Low intensity (L). The percent body fat and RMR of all subjects were measured at every periods. The RMR was measured early in the morning following a 12-hour fast using MMX3B gas analyzer and blood sample were collected from the anticubital vein to investigate thyroid hormonal (T3, T4, Free T3, Free T4, & TSH) changes. All the RMR values were expressed as absolute value/BSA $(kcal/d/m^2)$. And We also analyzed mean energy expenditure for 30 minutes during and after different intensity exercise. There was significant difference in RMR among different intensity of exercise. in TR (p < .05) not in the UT group. however, there was no significant different percent body fat in TR and in UT group. In the energy expenditure, there was significant different between TR and UT in HEE (high intensity exercise energy expenditure), LEE (low intensity exercise energy expenditure), HEEPE (high intensity exercise energy expenditure post exercise) & LEEPE (low intensity exercise expenditure post exercise). In the hormonal level, there was significant different in T4 level in the TR group at H period and in T4, Free T3, & Free T4 levels in TR group at L period, however there was no significant different in the UT group. The present cross-sectional study was design to investigate the relationship between exercise intensity and RMR. The focus of this investigation was to compare RMR in aerobically trained (TR) and untrained (VI). The relationship among RMR, exercise intensity and percent body fat would best be investigated using MMX3B and body composition analyzer. Each subject completed measurement of percent body fat, RMR, hormone in the period of maximal oxygen uptake exercise (M), high intensity exercise (H), and low intensity exercise (L). From the results, Low intensity of exercise (L), there was a trend for an increased RMR (kcal/day) in the TR not for the UT. This is best explained not by the reduced percent body fat but by the highly induced energy expenditure (during exercise and post exercise energy expenditure) and increased T4, Free T3, and Free T4 hormonal levels in the low intensity exercise for the TR group.

• Journal of the Korea Organic Resources Recycling Association
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• v.10 no.2
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• pp.92-99
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• 2002

The study was conducted to investigate the changes of soil properties and evaluation of plant utilization according to the application of compost used with phyllite. At this experiment, the plant cultivation test(lettuce and radish plant) was carried out to know about physico-chemical properties of compost, humus degradarion and stabilization degree, and some effects among rhe treatments were best at 20Mg/ha in PSPC 10(Phyllite, Saw dust, Pig manure Compost) plots and ar 10Mg/ha in PSPC 20 treatments, respectively. The EC value was high in the compost. Therefore the EC was appeared more than 2ds/m at the applied 40Mg/ha with compost. And in case of lettuce, Avail.-$P_2O_5$ and EC were more increased than in the radish plots, highly. In contrast, in case of radish, Toral-N, Total-C, and EC contents were decreased after plant cultivation. That is, it could know that radish was fond of absorprion of nitrogen. Also, BD values of soil physical properties were decreased according to increasing of phyllite addition ratio, however water holding capacity(WHC) was increased. The plant test had not been darnaged through the application of compost used with phyllite in soil and there had not been worse on changing of soil physico-chemical properties. Consequently, phyllite composts have been thought on agricultural utilized possibility.

• International Journal of Oral Biology
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• v.34 no.1
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• pp.1-6
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• 2009

The purpose of the present study was to examine the role of peripheral nitric oxide (NO) pathways in the onset of interleukin (IL)-1$\beta$-induced mechanical allodynia in the orofacial area. Experiments were carried out on male Sprague-Dawley rats weighing 230-280 gm and surgical procedures were performed under pentobarbital sodium (40 mg/kg, i.p.). Under anesthesia, a polyethylene tube (PE10) was implanted into the subcutaneous area of one vibrissa pad, which enabled the injection of IL-1$\beta$ or other chemicals. We subcutaneously injected 50 ${\mu}L$ of IL-1$\beta$ into a vibrissa pad through the implanted polyethylene tube with a 100 ${\mu}L$ Hamilton syringe. After the administration of 0.01, 0.1, 1, or 10 pg of IL-1$\beta$, withdrawal behavioral responses were examined. The subcutaneous injection of saline had no effects on the air-puff thresholds. Following the subcutaneous injection of 0.01, 0.1, 1, or 10 pg of IL-1$\beta$, the threshold of air puffs decreased significantly to 12 $\pm$ 3, 7 $\pm$ 2, 5 $\pm$ 1, or 5 $\pm$ 1 psi, respectively, in a dose dependent manner. Pretreatment with L-NAME, a nitric oxide synthase (NOS) inhibitor, blocked IL-1$\beta$-induced mechanical allodynia. However, neither D-NAME, an inactive isomer of L-NAME, nor vehicle affected the IL-1$\beta$-induced mechanical allodynia. Subcutaneous injection of IL-1$\beta$ increased the number of c-fos-like immunoreactive neurons, whereas pretreatment with L-NAME decreased this number, in the trigeminal caudal nucleus. These results suggest that pro-inflammatory cytokines and NO are important contributors to the pathogenesis of persistent and exaggerated IL-1$\beta$-induced pain states. Based on these observations, peripheral application of NOS inhibitors may be of therapeutic value in treating pain disorders in the clinic.

• Korean Chemical Engineering Research
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• v.46 no.4
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• pp.707-713
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• 2008

Characteristics of minimum fluidization velocity and pressure fluctuations were investigated in an annular fluidized bed whose diameter was 0.102 m and 2.0 m in height. Effects of gas velocity, particle size and bed temperature on the minimum fluidization velocity and pressure fluctuations were examined. The values of minimum fluidization velocity obtained by means of three different methods were very similar each other. The correlation dimension could be a quantitative parameter for expression the resultant complex behavior of gas and solid mixture in the annular fluidized bed. The value of correlation dimension increased with increasing gas velocity, fluidized particle size and temperature in the bed. The minimum fluidization velocity could be determined by means of correlation dimension of pressure fluctuations as well as pressure drop in the bed and standard deviation of pressure fluctuations. The minimum fluidization velocity increased with increasing particle size but decreased with increasing bed temperature in annular fluidized beds. The minimum fluidization velocity was well correlated in therms of correlation dimension as well as operating variables within experimented conditions of this study.

• Food Science and Preservation
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• v.5 no.4
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• pp.326-330
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• 1998

Freeze storage technique is widely used for food processing to keep freshness and quality of the product. This technique was applied to fresh, unshelled groundnut to develop a new type of product which could maintain fresh taste and nutritive values even after several months of storage. The groundnut varieties, Daepungtangkong, Daekwangtangkong and Sindaekwangtangkong were grown at the experimental field of NCES in 1996. Immatured pods or groundnut were harvested around 20 to 3o days before full maturity, washed, and steamed at 100$^{\circ}C$ for 5 min. to stop enzyme activity. After vacuum packing (at -760mmHg for 10 min.) with 0.08mm polyvinyldichloride film, the pods were immediately frozen at -70$^{\circ}C$ for 24h and transfered at -20$^{\circ}C$ for long-term storage. Physico-chemical properties of frozen vegetable groundnut were investigated at 2 months after storage and compared to those of conventionally dried groundnut. After 2 months storage, the thawed kernels were very palatable with softness and fresh taste. Acid value and hardness (measured as the compression force on the probe of a texture analyzer) were much lower in frozen vegetable groundnut than those in the air-dried ones. Presence of free sugars is one of the important factors affecting groundnut taste, and the free sugar contents were considerably decreased in the frozen vegetable groundnut compared to freshly harvested groundnut. But in dried groundnut no free sugar was detected.

• Korean Journal of Plant Resources
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• v.27 no.6
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• pp.687-700
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• 2014

Rice blast (Magnaporthe oryza B.) is one of the most important diseases in rice that causing great yield losses every year around the world. It is important to screen valuable genetic resources for improving blast resistance. This study was conducted to identify the blast resistance in 279 Korean rice landraces using blast nursery tests and isolate inoculum screening. The results showed that 11 landrace accessions found to be resistant to rice blast in blast nursery and inoculation screening tests and the degree of lesions in most accessions showed that they were susceptible to reactions. In order to find the distribution of blast resistant genes, a molecular survey was conducted to identify the presence of major blast resistance (R) gene in 279 Korean landraces. The results revealed that their frequency distribution was Pik-m (36.2%), Piz (25.4%), Pit (13.6%), and Pik (10%). Besides, the frequency distribution of Piz-t, Pii, Pik-m/Pik-p, Pi-39(t), Pib, Pi-d(t)2, Pita/Pita-2 and Pi-ta genes were identified as less than 10%. The results did not consist with the reactions against blast diseases between genotypes and phenotypic part of the nursery tests and isolate inoculation. For concluding these results, we used genome-wide SSR markers that have closely been located with resistance genes. The PCoA analysis showed that the landrace accessions formed largely two distinct groups according to their degree of blast resistance. By comparing genetic diversities using polymorphic information contents (PIC) value among the resistant, total and susceptible landraces, we found that PIC values decreased in four SSR markers and increased in six markers in the resistant accessions, which showed contrary to total and susceptible groups. These regions might be linked to resistance alleles. In this study, we evaluated the degree of blast resistance and the information about the distribution of rice blast resistant genes in Korean rice landraces. This study might be the basis for association analysis of blast resistance in rice.

• Environmental Analysis Health and Toxicology
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• v.17 no.3
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• pp.197-205
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• 2002

Volatile organic compounds (VOCs) are an important public health problem throughout the world. Many important questions remain to be addressed in assessing exposure to these compounds. Because they are ubiquitous and highly volatile, special techniques must be applied in the analytical determination of VOCs. Personal exposure measurements are needed to evaluate the relationship between microenvironmental concentrations and actual exposures. It is also important to investigate exposure frequency, duration, and intensity, as well as personal exposure characteristics. In addition to air monitoring, biological monitoring may contribute significantly to risk assessment by allowing estimation of absorbed doses, rather than just the external exposure concentrations, which are evaluated by environmental and personal monitoring. This study was conducted to establish the analytic procedure of VOCs in air, blood, urine and exhaled breath and to evaluate the relationships among these environmental media. The subjects of this study were selected because they are occupationally exposed to high levels of VOCs. Environmental, personal, blood, urine and exhalation samples were collected. Purge ＆ trap, thermal desorber, gas chromatography and mass selective detector were used to analyze the collected samples. Analytical procedures were validated with the“break through test”, 'quot;recovery test for storage and transportation”,“method detection limit test”and“inter-laboratory QA/QC study”. Assessment of halogenated compounds indicted that they were significantly correlated to each other (p value < 0.01). In a similar manner, aromatic compounds were also correlated, except in urine sample. Linear regression was used to evaluate the relationships between personal exposures and environmental concentrations. These relationships for aromatic and halogenated are as follows: Halogen $s_{personal}$ = 3.875+0.068Halogen $s_{environmet}$, ($R^2$= .930) Aromatic $s_{personal}$ = 34217.757-31.266Aromatic $s_{environmet}$, ($R^2$= .821) Multiple regression was used to evaluate the relationship between exposures and various exposure deter-minants including, gender, duration of employment, and smoking history. The results of the regression model-ins for halogens in blood and aromatics in urine are as follows: Halogen $s_{blood}$ = 8.181+0.246Halogen $s_{personal}$+3.975Gender ($R^2$= .925), Aromatic $s_{urine}$ = 249.565+0.135Aromatic $s_{personal}$ -5.651 D.S ($R^2$ = .735), In conclusion, we have established analytic procedures for VOC measurement in biological and environmental samples and have presented data demonstrating relationships between VOCs levels in biological media and environmental samples. Abbreviation GC/MS, Gas Chromatography/Mass Spectrometer; VOCs, Volatile Organic Compounds; OVM, Organic Vapor Monitor; TO, Toxic Organicsapor Monitor; TO, Toxic Organics.

• The Korean Journal of Pesticide Science
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• v.5 no.2
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• pp.1-12
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• 2001

A competitive indirect enzyme-linked immunosorbent assay (ci ELISA) for the organophosphorus insecticide acephate, O,S-dimethyl acetylphosphoramidothioate, was developed using a polyclonal antibody. Three different haptens mimicking the analyze and containing hexanoic acid moiety as a linker were synthesized, and then conjugated with the carrier proteins bovine serum albumin and keyhole limpet hemocyanin by the N-hydroxysuccinimide active ester method. Polyclonal antibodies raised against hapten-KLH conjugates in rabbits and the hapten-BSA conjugates as coating antigens were screened and selected for the assay in the homologous and/or heterologous ELISA system. The effects of various assay conditions, including blocking reagents, detergent content, organic solvents, pH, and preincubation of tile mixture of the polyclonal antibody and the analyze on the sensitivity were evaluated. The $IC_{50}$ value of acephate of 110 ng/mL was obtained in an optimized heterologous system using hapten-3-BSA as a coating antigen and a polyclonal antibody 8377, showing the detection range of 10-1000 ng/mL and the lowest detection limit of 4 ng/mL. The cross-reactivities of the structurally related insecticides, including methamidophos were less than 0.02%. These results indicate that the ELISA could be a convenient and alternative tool for monitoring acephate residues in agricultural products and environmental samples.

• Journal of Environmental Health Sciences
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• v.45 no.1
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• pp.30-41
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• 2019

Objectives: The effects of particulate matter and ozone on health are being reported in a number of studies. These effects are likely to be stronger on the elderly population, but studies in this regard are scarce. The purpose of this study was to examine the effects of particulate matter ${\leq}2.5{\mu}m$ and ozone on the acute health status of the elderly population. Methods: In order to analyze the health status of the elderly population, the NHIS-Senior Cohort data was used. In this study of people 60 years or older in Seoul, the number of outpatient visits and ER visits between 2002 and 2013 were calculated. Each disorder and the lag effect were analyzed separately. Particulate matter and ozone were analyzed using both the single exposure model and the adjusted multi-exposure model. Results: In the single exposure analysis with PM2.5 as the exposure variable, with each increase of $10{\mu}g/m^3$, the number of outpatient visits increased by 1.0081 times, vascular disease 1.0065 times, chronic pulmonary disease 1.0086 times, and diabetes 1.0055 times. In the multi-exposure model adjusting for ozone, the number of outpatient visits increased by 1.0066 times. There was a one-day lag effect and 1.0066 times increase between PM2.5 and ER visits in the multi-exposure model and 1.0057 times when adjusted for ozone (p value <0.10). There was a one-day lag effect in all multi-exposure models with ozone as the main variable, and when the particulate matter was adjusted, there was a one-day delay and 1.0143 times increase in ER visits. Conclusions: In our study, an increase in the number of outpatient and ER visits in the elderly population in accordance with the increase in PM2.5 and ozone was found. The association found in our study could also produce a socioeconomic burden. Future studies need to be performed in regards to younger populations and other air pollutants.

• Journal of the Korean GEO-environmental Society
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• v.23 no.12
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• pp.5-15
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• 2022

Conventionally, because evaluation methods of the bearing capacity for double steel pipe-concrete composite pile design have not been established, the conventional vertical bearing capacity equations for steel hollow pile are used. However, there are severe differences between the predictions from these equations, and the most conservative one among vertical bearing capacity predictions are conventionally adopted as a design value. Consequently, the current prediction method for vertical bearing capacity of composite pile prediction composite pile causes design reliability and economical feasibility to be low. This paper investigated mechanical behaviors of a new composite pile, with a cross-section composed of double steel pipes filled with concrete (DSCT), vertical bearing capacities were analyzed for several DSCT pile conditions. Axisymmetric finite element models for DSCT pile and surrounding ground were created and they were used to analyze effects on behaviors of DSCT pile pile by embedding depth, stiffness of plugging material at pile tip, height of plugging material at pile tip, and rockbed material. Additionally, results from conventional design prediction equations for vertical bearing capacity at steel hollow pile tip were compared with that from numerical results, and the use of the conventional equations for steel hollow pile was examined to apply to that for DSCT pile.