• 한국수정란이식학회지
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• 제31권3호
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• pp.299-305
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• 2016

Although cryopreservation of sperm is routinely used for clinical requirement, it has some problems, such as high generation of reactive oxygen species (ROS) and cold-shock. To reduce the detrimental damage in sperm, anti-oxidants were added to cryoprotectant for sperm. Curcumin is one of anti-oxidants, which are added in cryoprotectants. However, recent studies have demonstrated that curcumin decreases sperm viability and motility. This study was performed to identify the effect of curcumin on hydrogen peroxide ($H_2O_2$)-exposed bovine sperm, which were cryopreserved-thawed. In $H_2O_2$-exposed bovine sperm, reactive oxygen species (ROS) were significantly reduced by treatment with curcumin in a dose-dependent manner (p < 0.05). Among tested concentrations of curcumin (1 to $50{\mu}M$), 30 and $50{\mu}M$ curcumin showed anti-oxidant effect on $H_2O_2$-induced ROS generation. On the other hand, combination of 30 or $50{\mu}M$ curcumin with anti-oxidant $H_2O_2$ increased the percentage of apoptotic sperm compared to only $H_2O_2$ treatment. Sperm viability was also decreased in the combination of 30 or $50{\mu}M$ curcumin with $H_2O_2$ as judged by FDA/PI staining. $H_2O_2$-induced decrease in sperm progressive motility was recovered by treatment with $1{\mu}M$ curcumin. These results show that high concentration of curcumin has anti-oxidant effect, but it has also cytotoxic effect on bovine sperm. Sperm viability and motility might be more affected by cytotoxic signals of curcumin compared to antioxidant signals.

• Journal of Preventive Medicine and Public Health
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• 제13권1호
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• pp.13-18
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• 1980

The ultimate goal of treatment of carton monoxide poisoning is to promote dissociation of carboxyhemoglobin and to maintain arterial $PO_2$ above 50mmHg throughout the course of treatment to protect vital organs from damage caused by hypoxia. The hyperbaric chamber designed and manufactured for this purpose has obviousely made an enormous contribution and yet has several handicaps to be overcome by any means. These handicaps are: the financial impact to purchase the chamber (especially in a small, remote community), an extra manpower requirement to operate the device, limitation in the capacity of the chamber (one man type), and the possible hazard of oxygen intoxication and dysbarism. The primary objective of this study is to develope a new therapeutic measure as an alternative to the hyperbaric chamber when it is not available or contraindicated. The effect of intestinal perfusion with hydrogen peroxide has been studied by many investigators and was known to be an excellent way of extrapulmonary oxygen supply. the advantage of this method will include; 1) much more amount of oxygen is delivered to the tissue than one would expect from 100% saturation with oxygen at 1 ata, 2) the procedure is simple and most economical, 3) neither sophisticated equipment nor extra manpower is required. As a study preliminary to the clinical application, authors conducted a series of experiment to observe the effect of hydrogen peroxide enema on dissociation of carboxyhemoglobin in intoxicated rabbit blood. Using an animal gas chamber, 20 rabbits were exposed to CO gas of 6,000 ppm for 60 minutes. Ten rabbits of control group were given 10cc of warmed normal saline solution by reactal perfusion and for the other 10 of the experimental group, the same amount of 1% $H_{2}O_{2}$ solution was given by the same way. Two blood specimens were drawn from each rabbit: the first one immediately following the exposure and the second one after rectal perfusion, about 30 minutes after the first sampling. The result was as follows; 1) The decrease in carboxyhemoglobin concentration during the first 30 minutes in the control and experimental group were $18.18{\pm}4.49%\;and\;23.03{\pm}4.13%$ respectively shelving the significant difference (p<0.05) between the two groups. 2) Hemoglobin and hematocrit value showed no significant difference between two groups and not altered significantly by intestinal perfusion with $H_{2}O_{2}$.

• 한국환경농학회지
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• 제18권4호
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• pp.310-315
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• 1999

본 연구에서는 생물막법의 일종인 흡수성 Biofilter 시스템(ABS)을 설치하여 14개월의 실험기간 동안 건국대 농생대 구내의 염기성 부패조로부터 유입수를 3가지 수리부하율로 처리하여 시험하였다. 모니터링 결과에 의하면 ABS는 평균농도 $BOD_5=6.2mg/l$, TSS=4.4mg/l 이라는 아주 낮은 유기성 유출수를 연속적으로 발생시킬 수 있음이 확인되었다. 이러한 저농도 유출수는 현재 우리나라 오분법의 특정지구(상수원 보호구역)에 대한 방류수 기준($BOD_5=10mg/l$, TSS=10mg/l)에도 훨씬 못 미치는 양호한 농도로서 ABS의 우수한 유기성오수 처리성능이 입증되었다. 특히 유출수내의 TSS 농도가 지극히 낮아서 슬러지의 발생이 거의 없으므로 침전조를 생략할 수 있어 설치비용이 저렴해지고, 유지관리가 단순한 장점을 지니고 있음을 알 수 있었다. 그러나 ABS시설이 농촌에 널리 보급되어 실용적인 시스템이 되기 위해서는 앞으로 T - N과 T- P의 처리효율 향상 방안, 겨울철에 대한 Biofilter탱크내 임계온도 유지문제, 광역모니터링 및 신속보수 유지관리체계 확립에 대한 계속적인 연구가 필요하다. 이러한 문제점만 어느정도 해결된다면 ABS는 우리나라 농촌의 소규모 현장 오수처리시설로서 앞으로 미래가 밝은 차세대형 시스템이 될 것으로 확신한다.

• Journal of Preventive Medicine and Public Health
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• 제37권1호
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• pp.80-87
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• 2004

Objectives: To evaluate the learning achievement and satisfaction levels for the Field Epidemiology Specialist Training Program (FESTP), on infectious disease control between March 19 and October 31, 2002. Methods : The FESTP was designed as a set of 84 hours curricula including lectures, discussions, self-studies, and field practicals, and organized both centrally and locally by the Division of Communicable Disease Control of the National Institute of Health and 11 universities. Before and after the program, a questionnaire survey on the educational need (49 items) and satisfaction (15 items) was conducted on 484 trainees, who were responsible for communicable disease control and immunization at 242 regional health centers. The data were analyzed with paired t-tests for comparison of the educational needs between the pre and post scores. Results : The average score for satisfaction was 3.06 out of 5.0; with relatively higher scores for sincerity (4.10) and professionalism (4.01) of the tutors, adequacy (3.54) and clearness (3.51) of the evaluation criteria, usefulness (3.54) and fitness (3.52) of the contents, but with relatively lower satisfaction for schedule (2.96) and self-studies (2.91). The average for requirement for education improved, as shown by the decrease from 2.72 to 2.22 (p<.0001) with the biggest decrease in the outbreak investigation from 2.60 to 2.08. Conclusion : The FESTP was evaluated as being effective, the trainees showed moderate satisfaction and decrease educational needs. However, the actual schedules and self-studies should be rearranged to improve the satisfaction level.

• 한국지역사회생활과학회지
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• 제18권1호
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• pp.161-175
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• 2007

It is important for young adults to establish good dietary behavior for healthy life. The prevalence of obesity in college students has increased gradually. The obesity is one of the most prevalent health problems in S. Korea. Moreover, obesity is closely related with the disease such as cardiovascular disease, diabetes mellitus. The purpose of this study was to evaluate health practice, serum components and nutrient intake of the college students according to Body Mass Index (BMI) level. 400 male and female freshmen students in 4 year university located near to Seoul metropolitan area were surveyed respectively through the health check-up procedure for college entrance in February. Survey was carried out for them by questionnaires, including a 24-hour dietary recall and anthropometric measurements. Blood samples were obtained while fasting. Nutrient intake was expressed by DDS(Dietary Diversity Score by 5 food groups) and DVS(Dietary Variety Score). Nutrition level was analyzed by Can-Pro (Korea Nutrition Association). And for the quantity intake, percentage ratio against daily requirement(by KDRIs) and MAR(Mean Adequacy Ratio) were calculated. Underweight group($BMI<18.5kg/m^2$), normal weight group($18.5) and overweight group($BMI<23.0 kg/m^2$) were 18.5%, 61.2% and 19.3% respectively. Nutrient intake levels and food intake status were not significantly different among three groups. The mean nutrient adequacy ratio (MAR) was not significantly different by BMI status. Nutrient intakes of calcium, iron, and riboflavin were especially low for all the students. Overweight group showed high blood pressure(120.9/79.3 mmHg) compared with the underweight group (116.4/ 75.8mmHg) but the difference was not significant. The serum triglyceride level in overweight group (94,0mg%) was higher than that in normal (70.9mg%) or underweight group(70.8mg%)(p<0.05). HDL-cholesterol level in overweight (43.8 mg%) group was not significantly different from that in underweight group (45.9mg%). BMI was positively correlated with blood pressure, hemoglobin, and the intake of total fat and cholesterol. But it was negatively correlated with HDL-cholesterol level. With these results overweight group is closely related with the cardiovascular disease risks, such as high blood pressure and high triglycerides and cholesterol. Thus, a health intervention program including weight control is required for young adults.

• 대한교통학회지
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• 제22권2호
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• pp.131-143
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• 2004

최적경로 알고리즘에서 링크표지를 활용하면 도시가로망 상에 나타나는 유턴이나 피턴과 같은 주행했던 교차로를 다시 주행하는 통행에 대한 설명이 가능하다. 본 연구에서는 링크표지기법을 표지갱신기반 다수경로탐색알고리즘으로 확대하는 것이 목적이다. 이를 위해 도시가로망에서 발생하는 운전자의 합리적 통행행태를 링크기반으로 개념화하고, 이들 행태가 반영되도록 링크표지갱신 다경로 알고리즘을 제안하였다. 소규모 네트워크 테스트를 통하여 알고리즘의 수행과정과 결과의 적정성을 확인하였다. 대규모 네트워크 컴퓨터 수행을 통해 해의 퇴화현상을 파악하고 소수의 대안경로 정보제공에 활용 가능한 (K-1)차원 알고리즘을 제안하였다. 결론적으로 제안된 다수경로 알고리즘으로 링크표지기법이 제공하는 합리적 통행행태의 고려가 다수의 경로에도 가능해졌을 뿐만 아니라, 효율적인 대안경로 제공의 활용단계를 위해 한단계 전진되었다.

• Journal of Chest Surgery
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• 제13권2호
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• pp.85-91
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• 1980

Extracorporeal circulation by hemodilution technique has been currently used with its clinical safety and good peripheral tissue perfusion in open heart surgery. There is no doubt that $O_{2}$ carrying capacity of the blood is disturbed by decreased hemoglobin level resulting from hemodilution of the circulating blood. From the view point of the blood gas exchange, these experimental studies were undertaken to determined the sate limit of hemodilution in the condition of cardiopulmonary bypass with a constant perfusion flow rate. Twelve adult mongrel dogs weighing 10 to 13 Kg. were anesthetized with pentobarbital and then respiration was controlled with Harvard volume respirator using room air. The cardiopulmonary by pass was performed by use of Sarns heart lung machine (console 5000, 5 head and 2 roller pumps) and Travenol pediatric bubble oxygenator. The perfusion rate during bypass was maintained at a constant rate of 80 ml/min/Kg of body weight. The ratio of oxygen gas flow to blood flow was kept in 3 to 1 constantly. International hemodilution was attained by serial blood withdrawals and immediate infusion of equal volumes of diluants composed of Ringer's lactate, 5% dextrose in water and 25% mannitol solution, proportionally 60%, 30%, and 10%. Arterial and venous blood samples were obtained between 15 and 20 minutes following each hemodilution. Hematocrits and hemoglobin values, $PO_{2}$, $PCO_{2}$ and pH were measured. Oxygen and carbon dioxide contents oxygen consumption and carbon dioxide elimination were calculated groups according to different hematocrit values and the correlations were evaluated. Result were as follows. 1. the arterial $O_{2}$ tension and $O_{2}$ saturation were maintained at the physiological level irrespective of the hematocrit value. 2. The venous $O_{2}$ tension and $O_{2}$ saturation showed a tendency to decline with the decrease in hematocrit value and positive correlation between them (r = +0.49, r = +0.76), The mean values of venous $O_{2}$ tension and $O_{2}$ saturation, however, were not decreased when the hematocrit levels were lower than 20%. 3. The arterial $O_{2}$ content declined lineally in proportion to the fall of hematocrit level with a positive correlation between them (r = +0.95). 4. The venous $O_{2}$ contents were decreased gradually as the hematocrit value decreased with positive correlation between them ( r =+0.89). The trend of diminution of venous $O_{2}$ content, however, was became low according to progressive decrease of hematocrit level. 5. Systemic oxygen consumption was in higher range than $O_{2}$ requirement of basal metabolism when the hematocrit value was above 20%, but abruptly decreased when the hematocrit value became to below 20%. 6. The arterial $CO_{2}$ tension and $CO_{2}$ content showed trend of increasing with progressive decrease of hematocrit value but exhibited a rather broad range and there was no relationship between those value and the hematocrit value. 7. The venous $CO_{2}$ tension and $CO_{2}$ content have also no correlation with change of Ht. value but related directly to those value of arterial blood with positive correlation between them (r = +0.78, r = +0.95_. 8. A-V difference of $CO_{2}$ content and $CO_{2}$ elimination wasnot significantly influenced by Ht. value. From the results, we obtained that feasible limit in inteneional hemodilution is above the hematocrit value of 20% under the given experimental condition.

• 대한지역사회영양학회지
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• 제14권2호
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• pp.190-205
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• 2009

The purposes of the study were to identify knowledge and skill levels required for effective nutrition teachers and to compare perceived need and dietitians' self-evaluation of the knowledge and skills. A total of 60 knowledge statements and 70 skill statements associated with 11 job functional areas were specified through a literature review and expert panel reviews. A total of 457 dietitians working at school foodservices in Seoul and Gyeonggi province were surveyed using a self-administrated questionnaire and 148 responses were returned. Excluding responses with significant missing data, 142 responses were used for data analysis. In terms of knowledge, 'sanitation, food safety and employee safety(4.60)' category received the highest perceived need score, followed by 'nutrition education(4.56)' and 'nutrition counseling(4.45).' The knowledge category that received the highest self-evaluation was 'nutrition and menu management(3.66)' while the category that received the lowest self-evaluation was 'teaching practices(2.83).' In terms of skills, the highest perceived need was associated with 'nutrition education(4.49)', followed by 'sanitation, food safety and employee safety(4.46)' and 'nutrition counseling(4.39).' The dietitians rated their skills related to 'sanitation, food safety and employee safety(3.67)' the highest but their skills related to 'teaching practices(2.84)' the lowest. The dietitians' self-evaluated knowledge and skill scores were significantly lower than their perceived need of the knowledge and skills in all job functional areas(p<0.001). A quadratic analysis based on the requirement and self-evaluation of the knowledge and skills revealed that priorities of the education programs targeting school nutrition teachers or students preparing to be a nutrition teacher should be placed on improving knowledge and skills related to nutrition education, nutrition counseling, teaching practices, sanitation and employee safety, and nutrition and menu management. Educational programs for nutrition teachers should be designed to decrease the gaps between the need and self-evaluation of the knowledge and skills for effective nutrition teachers. The findings of the study can be used to develop education materials for nutrition teachers. The knowledge and skills identified in the study should be updated and revised regularly to reflect changes in regulations and current practices in school foodservice programs.

• 한국환경농학회지
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• 제4권2호
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• pp.102-107
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• 1985

수도(水稻)에 대(對)하여 토양(土壤)에서 동(銅)의 농도(濃度)를 달리하고 개량제(改良劑)로서 무시용(無施用) 소석회(消石灰)(중화량(中和量)+150kg/10a) 및 규회석(硅灰石)(200 kg/10a)을 시용(施用)한 후(後)에 간단관수(間斷灌水)와 상시담수(常時湛水)로 조절(調節)하여 pot 시험(試驗)한 결과(結果)는 다음과 같다. 1) 수량(收量)은 동(銅) 처리농도(處理濃度)가 높을수록 감수(減收)되었으며 유의성(有意性)있는 토양중(土壤中) 감수농도(減收濃度)는 간단관수(間斷灌水) : 133.1 ppm 상시담수(常時湛水) : 136.8 ppm이었다. 2) 동(銅) 200 ppm인 토양(土壤)에서 소석회(消石灰) 시용(施用)으로 26${\sim}$30% 규회석(硅灰石) 시용(施用)으로 27${\sim}$48% 증수(增收)되었고 상시담수(常時湛水)하므로 간단관수(間斷灌水)보다 증수(增水)되었다. 3) 동(銅) 함량(含量)이 높을수록 수량(收量) 구성요소중(構成要素中) 주당(株當) 총수등열율(總數登熱率) 및 천립중(千粒重)이 감소(減少)되고 소석회(消石灰) 및 규회석시용(硅灰石施用)으로 수수(穗數)와 입수(粒數)가 적었다. 4) 동(銅) 함량(含量)이 높을수록 현미(玄米) 및 경엽중(莖葉中)의 동(銅) 함량(含量)이 증가(增加)되고 상시담수(常時湛水) 및 소석회(消石灰) 계규회석(桂灰石) 시용(施用)으로 그 함량(含量)이 적어졌다. 5) 토양중(土壤中) Cu/Ca+Mg 당량비(當量比)가 증가(增加)할수록 경엽중(莖葉中) 동(銅) 함량(含量)이 높았다. 6) 석회물질(石灰物質) 시용(施用)으로 토양(土壤) pH는 소석회(消石灰)>규회석(硅灰石)>무시용(無施用) 순(順)이었다. 7) 시험토양중(試驗土壤中) 동(銅) 함량(含量)은 상시담수(常時湛水)가 간단관수(間斷灌水)보다 높았다.

• Asian-Australasian Journal of Animal Sciences
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• 제13권11호
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• pp.1638-1652
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• 2000

Prolific females require better nutrition and feeding practice because of larger litter size and the substantial decline in body fat. Life-time pig output will be compromised if body protein and fat are not properly managed. First litter females are especially vulnerable because they can loose ${\geq}15%$ of whole-body protein. Conservation of body protein mass during first lactation minimizes wean to estrus interval and increases second litter size (up to 1.2 pigs). The ability to influence litter-size by amino acid nutrition is a new dimension in our understanding. A P2 fat depth below 12 mm at farrow and below 10 mm at wean compromised wean to estrus interval (>2 d) and next litter size (0.5 to 1.5 pigs) in sows. It is now clear that a 'modest' excess of feed during the first 72 h of pregnancy decreases embryo viability so that the potential for an increased litter size at birth is not realized. The capacity for milk production by prolific young sows is 25% higher than the standard used previously (NRC, 1988). First litter females averaged 9.82 kg milk/d for a 21 d lactation. Second and third litter counterparts averaged 10.35 kg/d. Milk production was 95% of peak by 10 d of lactation and sows were in greatest negative energy and lysine balance during the first 6 d. Nearly 45% of the total loss in body protein occurred within the first 6 d, but this could reduced to 30-35% by using a more aggressive feeding strategy after parturition. There appear to be 2 phases in lactation for lysine need (d 2-12 vs 12-21). Feeding to the higher level alleviates the second litter size decline. The lysine requirement for lactation can be predicted with accuracy, but we are not able to predict the second limiting amino acid. Mammary uptake of valine relative to lysine and recent work with practical diets suggest that the recent NRC (1998) pattern is realistic and that threonine and valine could be co-limiting for corn-soy diets for prolific sows nursing 10-11 pigs. Empirical studies are needed to refine the ideal pattern so that synthetic lysine can be used with more confidence. Milk fat output for the elite sow is extraordinary and poses an unnecessarily high energetic cost. Methods that reduce mammary fat synthesis will benefit the sow and may enhance piglet growth.