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Effect of Water Treatment Sludge Application on the Growth of Altari Radish(Raphanus sativus L.) (알타리무우 생육(生育)에 대한 정수(淨水) 슬러지의 시용효과(施用効果))

  • Chang, Ki-Woon;Kim, Sangdeog A.;Kim, Young-Han
    • Korean Journal of Soil Science and Fertilizer
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    • v.26 no.2
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    • pp.78-84
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    • 1993
  • In order to know the effect of sludge from water treatment plants un agricultural environment, an experiment of water treatment sludge application for Altari radish (Raphanus sativus L.) was carried out. And the results were as follows ; 1. The pH of water treatment sludge was 7.2, which was higher than that of the experimental soil. The exchangeable Ca content in the sludge was very high being 29me/100g sludge. So when the sludge was applied to the soil, amending effect for soil acidity was supposed. 2. With the application of the water treatment sludge to the soil, soil pH and organic matter content were increased. With the increase of the level of sludge application, contents of crude fiber and Ca in Altari radish were increased, white those of P and Mg tended to decrease. 3. The length of the root and the amount of fresh yield of the whole plant of radish tended to increase with the increase of sludge application, and this tendency was more evident on the infertile soil. From the above results, it was concluded that the fresh yield of Altari radish increased up to 75ton/ha sludge application on the fertile soil, but the yield decreased when higher levels of sludge was applied. While on the infertile soil, the yield increased up to the application of 300ton/ha, and the heavier application level did not adversely affect the yield of Altari radish.

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Typing and Sequence Analysis of the VP7 Gene of Rotavirus Isolated from Infants in Korea (한국영아에서 분리된 로타바이러스의 VP7 유전자형 및 염기서열 분석)

  • Song, Mi-Ok;Yun, Karyn;Chung, Sang-In;Choi, Chul-Soon;Lim, In-Seok;Kang, Shien-Young;An, Chang-Nam;Kim, Won-Yong
    • The Journal of Korean Society of Virology
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    • v.30 no.2
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    • pp.101-112
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    • 2000
  • Rotaviruses are the most common cause of severe vomiting and diarrhea in children worldwide and classified as a genus in the family Reoviridae. Rotavirus has eleven segmented dsRNAs and the virion consists of three shells. Outer capsid VP7 and VP4 induce neutralizing antibodies and are classified into G types (glycoprotein VP7) and P types (protease-sensitive VP4). Characterization of VP7 gene of Korean isolates of human rotavirus was performed using multiplex PCR and nucleotide sequence analysis. After RT-PCR amplification of full length (1,062 bp) of VP7 genes, the amplified PCR products were G typed by multiplex PCR and the nucleotide sequences were compared with those of reference rotavirus from GenBank. The G type analysis revealed that 25% (2/8) belong to G1, whereas 37.5% (3/8) benong to G2 and G4, respectively. The Korean isolates within the same serotypes showed high homology of nucleotide sequences and could be discriminated from foreign isolates exception with two strains (CAU009 and CAU022). But Korean isolates CAU009 and CAU022 were close related into japanease isolates 417 (99.2%) and indian isolates (97.6%) than Korean isolatese. Our results showed that these two strains were supposed to be originated from abroad. As a results, The G typing and nucleotide sequence analysis of VP7 gene of rotavirus isolated from infants in Korea could be used for identification, serotying and determination of novel or unusual strains of rotaviruses.

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Evaluation of Particle Size Effect on Dynamic Behavior of Soil-pile System (모래 지반의 입자크기가 지반-말뚝 시스템의 동적 거동에 미치는 영향 평가)

  • Han, Jin-Tae;Yoo, Min-Taek;Yang, Eui-Kyu;Kim, Myoung-Mo
    • Journal of the Korean Geotechnical Society
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    • v.26 no.7
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    • pp.49-58
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    • 2010
  • This paper presents experimental results of a series of 1-g shaking table model tests performed on end-bearing single piles and pile groups to investigate the effect of particle size on the dynamic behavior of soil-pile systems. Two soil-pile models were tested twice: first using Jumoonjin sand, and second using Australian Fine sand. In the case of single-pile models, the lateral displacement was almost within 1% of pile diameter which corresponds to the elastic range of the pile. The back-calculated p-y curves show that the subgrade reaction of the Jumoonjin-sand-model ground was larger than that of the Australian Fine-sand-model ground at the same displacement. This phenomenon means that the stress-strain behavior of Jumoonjin sand was initially stiffer than that of Australian Fine sand. This difference was also confirmed by resonant column tests and compression triaxial tests. And the single pile p-y backbone curves of the Australian fine sand were constructed and compared with those of the Jumoonjin sand. As a result, the stiffness of the p-y backbone curves of Jumunjin sand was larger than those of Australian fine sand. Therefore, using the same p-y curves regardless of particle size can lead to inaccurate results when evaluating dynamic behavior of soil-pile system. In the case of the group-pile models, the lateral displacement was much larger than the elastic range of pile movement at the same test conditions in the single-pile models. The back-calculated p-y curves in the case of group pile models were very similar in both sands because the stiffness difference between the Jumoonjin-sand-model ground and the Australian Fine-sand-model ground was not significantly large at a large strain level, where both sands showed non-linear behavior. According to a series of single pile and group pile test results, the evaluation group pile effect using the p-multiplier can lead to inaccurate results on dynamic behavior of soil-pile system.

Transformation of an Alkalin Protease Overproducer, Vibrio metschnikovii Strain RH530, and Improvement of Plasmid Stability by the par Locus

  • Chung, So-Sun;Shin, Yong-Uk;Kim, Hee-Jin;JIn, Chee-Hong;Lee, Hyune-Hwan
    • Journal of Microbiology and Biotechnology
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    • v.11 no.2
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    • pp.222-228
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    • 2001
  • Vibrio metschnikovii strain RH530 is a non-pathogenic, industrially-important alkaline protease producer which has been isolated from wastewater. In this paper, we report on the transformation of this strain by using the method of electroporation. A field strength of $7.5\;kVcm^{-1}$ and $25\;{\mu}F$, and using a 0.2-cm cuvette, appeared to be the optimal conditions for electroporation of the cells with the recombinant pSBCm plasmid carrying the vapK alkaline protease gene and the ColE1 replicon. Cells were subjected to osmotic shock in order to remove extracelluar DNase, and adding 200 mM of sucrose to electroporation buffer cells showed an increased transformation efficiency. Maximum efficiency of transformation was obtained at an early exponential growth phase. Using all of the conditions mentioned above, we routinely obtained a transformation efficiency of more than $10^4{({\mu}g\;plasmid\;DNA)}^{-1}$. The stability of the plasmid pSBCm in V. metschnikovii RH530 was 25% after 18h of growth (27 generations) in the medium without antibiotic selection. The insertion of the par locus to the pSBCm increased the stability of the plasmid up to 42% without selective pressure. The increase in plasmid stability was accompanied by the increase in the productivity of alkaline protease in the recombinant V. metschnikovii strain RH530. Determining optimal conditions for the transformation of the industrially-important, nonpathogenic Vibrio strain, and the improvement of plasmid stability by introducing the par locus into the high copy number plasmid vector, will allow the development of procedures involved in the genetic manipulation of this strain, particularly for its use in the production of industrial enzymes such as alkaline protease.

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Changes in the Cervical and Lumber Flexion-Relaxation Ratio, Range of Motion, Pressure Pain Threshold, and Perceived Comfort Following the Wearing of a Trunk Brace during Smartphone Watching (스마트폰 시청 시 몸통 교정기 착용에 따른 목, 허리 굽힘-이완 비율, 관절가동범위, 압통, 착용감 변화)

  • Park, Jin-Seong;Park, Du-Jin
    • PNF and Movement
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    • v.19 no.3
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    • pp.413-422
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    • 2021
  • Purpose: This study was conducted to investigate changes in the cervical and lumbar flexion-relaxation ratio, range of motion, pressure pain threshold, and perceived comfort following the wearing of a trunk brace during smartphone watching. Methods: To calculate the number of subjects for this study, an analysis with G*Power was performed at a statistical power of 0.8, an effect size of 0.5, and a significance level of 0.05, based on the results of a preliminary experiment on five subjects. In total, 27 adult men and women were recruited who had been informed of the study's purpose and process and had agreed to participate. All subjects watched content on a smartphone for 20 minutes in the same posture and conditions while wearing and not wearing a trunk brace, and then their cervical and lumbar flexion-relaxation ratio, range of motion, pressure pain threshold, and perceived comfort were measured. Results: Compared to the non-wearing of a trunk brace, the wearing of a trunk brace resulted in a statistically significant smaller decline in cervical extension and right-side cervical rotation (p<0.05). When the subjects wore a trunk brace, their right- and left-side cervical and right-side lumbar pressure pain statistically significantly improved when compared to not wearing a trunk brace (p<0.05). They also perceived a significantly lowered level of comfort 20 minutes after wearing a trunk brace compared to immediately after wearing it (p<0.05). Conclusion: The trunk brace was effective in reducing declines in right-side cervical rotation and the occurrence of left- and right-side cervical and right-side lumbar pressure pain. The findings indicate the need to improve the perceived comfort of trunk braces.

Adsorption of Heavy Metals by Natural Adsorbents of Green Tea and Ginseng Leaves (녹차잎과 인삼잎의 중금속 흡착능 평가 연구)

  • Kim, Sohyun;Song, Jinyoung;Yoon, Kwangsuk;Kang, Eunmi;Song, Hocheol
    • Journal of Soil and Groundwater Environment
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    • v.22 no.5
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    • pp.128-134
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    • 2017
  • This work presents the adsorption capability of green tea and ginseng leaves to adsorb heavy metals such as Cd(II), Cu(II), and Pb(II) in aqueous solution. FT-IR analysis indicates the presence of oxygen containing functional groups (carboxyl groups) in two kinds of leaves. High pH condition was favorable to the adsorption of heavy metal ions due to the enhanced electrostatic attraction and the precipitation reaction of metal ions. The adsorption of Cd(II), Cu(II), and Pb(II) reached equilibrium within 10 min, achieving high removal efficiencies of 80.3-97.5%. The adsorption kinetics data of heavy metal ions were fitted well with the pseudo-second-order kinetic model. The maximum adsorption amounts of Cd(II), Cu(II), and Pb(II) ions were 8, 3.5, and 15 mg/g, respectively, in the initial concentration range from 0.15 to 0.75 mM. Based on the fitting data obtained from isotherm models, heavy metal adsorption by green tea and ginseng leaves could occur via multi-layer sorption.

Structural and Optical Properties of Er(III) Complex with ODA and Phen (ODA = Oxydiacetate, Phen = 1,10-Phenanthroline)

  • Kang, Jun-Gill;Kim, Tack-Jin;Park, Kwan-Soo;Kang, Sung-Kwon
    • Bulletin of the Korean Chemical Society
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    • v.25 no.3
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    • pp.373-376
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    • 2004
  • A novel Er(III) complex with oxydiacetate and 1,10-phenanthroline was synthesized and its structure and luminescence properties were characterized. The complex of $[Er(ODA){\cdot}(phen){\cdot}4H_2O]^+$ crystallizes in the monoclinic space group $P2_1$/n with a = 12.216(4) ${\AA}$, b = 16.680(2) ${\AA}$, c = 12.627(3) ${\AA}$, ${\beta}=108.30(2)^{\circ}$, V = 2442.7(11) ${\AA}^3$, Z = 4 and ${\rho}=1.841 g/cm^3$. When the complex is excited at the He-Cd 325-nm line, it produces two broad bands spanning the regions 350-650 nm and 1200-1650 nm. The emission band of the complex is characterized by a series of spectral dips in the visible emission profile. The complex exhibits sensitized near- IR emission via two kinds of energy transfers from phen to Er(III): nonradiative and radiative energy transfers.

The Solvent-Independent Structure of Piroxicam (피록시캄의 용매 비의존 결정구조)

  • Kim, Bong-Hee;Suh, Il-Hwan;Jhee, Ok-In;Suh, Jong-Myung;Suh, Jung-Jin
    • Journal of Pharmaceutical Investigation
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    • v.18 no.4
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    • pp.209-215
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    • 1988
  • The three-dimensional structures of piroxicam crystallized from two different solvents, toluene and toluene/hexane mixture respectively, are proved identical: $C_{15}H_{13}N_3O_4S,\;M\;=\;331.35$, monoclinic, a = 7.128(1), b = 15.146(2), c = 13.956(2) ${\AA},\;{\beta}=\;97.33(1)^{\circ},\;V\;=\;1494.37{\AA}^{3},\;Dx\;=\;1.472\;g/cm^{3},\;Z\;=\;4,\;space\;group\;P2_{1}/c,\;Mo\;K{\alpha}(\lambda=\;0.71073\;{\AA})$, F(000) = 688, T = 295 K, R = 0.0611 for 1993 unique observed reflections. The thiazine ring exhibits a half chair conformation. An amide group is involved in an intramolecular hydrogen bond to the hydroxy group, O(17)-H(17)${\cdots}O(15){\AA}$. The molecule is planar within 2 ${\AA}$ with the interplanar angle $127.9(4)^{\circ}$ between pyridine and benzene rings. A molecular chain parallel to [011] is formed by two intermolecular hydrogen bonds N(16)-H(6)${\cdots}O(11)$ and C(6)-H(6)${\cdots}O(11)$, and the molecular chains are held together by van der Waals forces.

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THE INFLUENCE OF THE DIE HARDENER ON GYPSUM DIE (석고 다이에 대한 다이 강화제의 영향)

  • Kim, Young-Rim;Park, Ju-Mi;Song, Kwang-Yeob
    • The Journal of Korean Academy of Prosthodontics
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    • v.45 no.4
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    • pp.546-554
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    • 2007
  • Statement of problem: Die materials require abrasion resistance, dimensional stability with time, and high surface wettability for adequate material properties. Wear of gypsum materials is a significant problem in the fabrication of accurately fitting cast prosthetic devices. So It has been recommended that the use of die hardener before carving or burnishing of the wax pattern. Purpose: The purpose of this study was to compare the abrasion resistance and surface microhardness(Knoop) with 3 commonly used gypsum die materials(MG Crystal Rock, Super plumstone, GC $FUJIROCK^{(R)}$ EP) with and without the application of 2 die hardeners. Material and methods: Three die materials were evaluated for abrasion resistance and surface microhardness after application of 2 die hardeners(Die hardener and Stone die & plaster hardener). Thirty specimens of each gypsum material were fabricated using an impression of resin die(Pattern resin; GC Corporation, Japan) with 1-mm high ridges, sloped 90 degrees. Gypsum materials were mixed according to manufacturer's recommendations and allowed to set 24 hours before coating. Specimens were arbitrary assigned to 1 of 3 treatment subgroups (n=10/subgroup): no treatment(control), coated with Die hardener, and coated with Stone die & plaster hardener. Abrasion resistance(measured by weight loss) was evaluated using device in 50g mass perpendicular to the ridges. Knoop hardness was determined by loading each specimen face 5 times for 15 seconds with a force of 50g. A scanning electron microscope was used to evaluate the surface of specimens in each treatment subgroup. Conclusions: The obtained results were as follows: 1. 3 types of die stone evaluated in this study did not show significant differences in surface hardness and abrasive resistance(P<.05). 2. In the abrasive resistance test, there were no significant differences between GC $FUJIROCK^{(R)}$ EP and MG Crystal Rock with or without 2 die hardener(P<.05). 3. Super plumstone treated with Stone die & plaster hardener showed increased wear loss(P<.05) 4. Die hardener coatings used in this study decreased the surface hardness of the gypsum material(P<.05).

A Study on Self-Esteem and Urinary Symptoms in Women with Urinary Incontinence (요실금환자의 골반근육운동에 따른 자아존중감과 배뇨증상에 관한 연구)

  • Lee Young-Hee;Kim Sun-Are
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.9 no.3
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    • pp.360-369
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    • 2002
  • Purpose: The purpose of this study was to help enhance the quality of life for women with urinary incontinence. Self-esteem, urinary symptoms, peak pressure and duration in seconds for vaginal contraction after pelvic muscles exercise were examined. Method: One-group pretest-posttest design was employed participants were 27 married women from G city. The instrument for this study were : the self-esteem scale developed by Rosenberg(1965). the Urinary Symptom Questionnaire, a subjective measurement of urinary incontinence, developed by Jackson et al.(1996) and the Perineometer used as an objective measurement of peak pressure and duration in seconds. Result: 1. Self-esteem showed significant improvement after the exercise (t=-3.832, p= .001). 2. Comparison of results before the pelvic muscles exercise and after showed that there was a statistically significant difference for several urinary symptoms including enuresis (t=2.833, p=.009), frequency of incontinence (t=2.964, p= .006), incontinence volume (t=2.280, p= .031), incontinence before getting to the restroom (t=3.035, p= .006), incontinence with no reason or feeling (t= 3.051, p= 005) burning sensation (t= 2.132, p=.043), and a sense of residual urine (t=2.267 p=.032). The mean scores showed improvement in the urinary symptoms of management of incontinence (M=$0.04{\pm}0.22$), daily frequency of incontinence management (M=$0.13{\pm}0.85$), urinary control (M=$0.15{\pm}0.86$), running to the restroom (M=$0.15{\pm}0.60$) incontinence caused by coughing or physical activities (M=$0.19{\pm}0.57$) and hesitancy (M=$0.07{\pm}0.55$). Overall urinary symptoms decreased significantly (t =3.073. p= .007). 3. Peak pressure showed an increase from a mean of $24.26{\pm}16.20mmHg$ before the exercise to a mean of $28.63{\pm} 17.79mmHg$ after (t=-2.399, p=.024). Duration in seconds also increased from a mean of $6.00{\pm}4.95sec$ to $9.15{\pm}5.83sec$ (t=-4.400, p= 000). Conclusion: These findings suggest that pelvic muscle exercise serves to decrease urinary problems, improve self-esteem and increase peak pressure and duration in seconds.

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