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Genetic Variation of Isozymes in Populations of Pinus densiflora and Pinus thunbergii Naturally Distributed in Kyungpook Province (경북지방(慶北地方) 소나무 및 곰솔집단(集團)의 동위효소(同位酵素)에 의한 유전변이(遺傳變異))

  • Son, Doo-Sik;Hong, Sung-Chun;Yeo, Jin-Kie;Ryu, Jang-Bal
    • Journal of Korean Society of Forest Science
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    • v.78 no.4
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    • pp.345-359
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    • 1989
  • This study was conducted to estimate genetic variation of Pinus densiflora in Kyungpook province which shows morphological variation between northern and southern types. Six isozymes sueh as IDH, ME, PGI, ADH, GOT and LAP was analyzed using megagametophyte tissue of seeds by electrophoresis and 16 populations of Pinus densiflora and 5 of Pinus thunbergii were tested. The results obtained were as follows ; 1. In P. densiflora, 13 Loci were found in 6 isozymes and 8 loci of them were polymorphic, having 24 alleles, while in Pinus thunbergii, there were 18 alleles detected from 6 loci among the same number of loci as in Pinus densiflora. 2. The genotypic frequency in Pinus densiflora vary slightly among populations for some isozymes, but most of them satisfied the law of Hardy-Weinherg equilibrium, while some populations such as Youngil for ADH and LAY, Youngyang for ADH and Bonghwa for LAY did not follow the law and showed high fixation index values and homozygosities higher than expected. 3. The variation among populations based on the genetic distance was small except populations of Youngju, Baegam, Gyungju and Sangju, however they could be clustered by three groups : northern Kyungpook group including Mt. Taebak, inland Kyungpook group represented by Sungju, Eusung and Kumleung population and coastal Kyungpook group represented by Baegam and Gyungju population. 4. No significant difference was found in 6 isozymes between northern and southern types of Pinus densiflora which was morphologically different. 5. The frequency of heterozygous loci per tree was higher in Pinus densiflora especially for ME-A and A DH-B, while that of homozygous loci was higher in Pinus thunbergii except for ADH-B, LAY-B and PGI-B locus.

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A Phytosociological Study of the Forest Communities in Mt. Palgong(I) -Pinus densiflora Forests- (팔공산(八公山) 삼림군락(森林群落)의 식물사회학적연구(植物社會學的硏究)(I) -소나무림(林)에 대해서-)

  • Cho, Hyun Je;Hong, Sung Cheon
    • Journal of Korean Society of Forest Science
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    • v.79 no.2
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    • pp.144-161
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    • 1990
  • Pine forest communities developed in Mt. Palgong, southeastern Korea, were studied phytosociologically, with special reference to multiple management of local forests, and were classified into two communities, Pinus densiflora - Quercus mongolica community(I : mountain forest) and P. densiflora-Climbing plants community (II : valley forest) and six subgroups accompanied by several subgroups. Judging from the coincidence method, the division of communities (vegetation units) was closely related to altitude and topography. Based on vegetation units, a vertical distribution map of pine forest communities was prepared. The species composition(%) of pine forest communities under stratification, in upper and lower tree layer, teas I higher than II, in middle and shrubs lacer II higher than I (Total : Upper 15.5%, Middle 28.4%, Lower 34.6%. Shrubs 21.5%. Sum of mean coverage%i of understory vegetation was II twice as high as I (Total. shrubs 28.4%. forbs 11.4%, Graminoids 11.8%, ferns 1.0%). Based on constance, coverage and d.b.h. class etc., an actual growth and occurrence table of tree species and understory vegetation by vegetation unity were prepared, and could assume a criterion for judging potential dominance-growth conditions.

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Effects of Light, Temperature, and Water Stress on the Photosynthesis and Respiration Rates of Leaves in Four Oak Species (4종(種)의 참나무엽(葉)의 광합성속도(光合成速度)와 호흡속도(呼吸速度)에 미치는 광(光), 온도(溫度), 수분(水分)의 영향(影響))

  • Han, Sang Sup;Kim, Ha Sun
    • Journal of Korean Society of Forest Science
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    • v.78 no.2
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    • pp.151-159
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    • 1989
  • The present study has been designed to define the effects of photosynthetically active radiation, leaf temperature, and water stress on photosynthesis and respiration of leaves of four oak species (Quercus mongolica, Quercus aliens, Quercus variabilis, and Quercus serrate). The results obtained are as follows : 1. The estimated light compensation points at which Pn approached zero were 38, 24, 20, and $18{\mu}Em^{-2}s^{-1}$ for Q. aliens, Q. variabilis, Q, mongolica, and Q. serrate, respectively. The light saturation points occurred at $500{\mu}Em^{-2}s^{-1}$ in three oak species except Q, aliens. 2. The maximum rates of Pn were 19.7, 15.2, 11.2, and 11.0 mg $CO_2$ $dm^{-2}h^{-1}$ for Q. variabilis, Q. serrate, Q. monglica, and Q. aliens leaves, respectively. 3. The transpiration rates of Q. variabilis and Q. serrate leaves were slightly higher than those of Q. mongolica and Q. aliens leaves at various photosynthetically active radiations(PAR), but cuticular transpiration rates at dark were similar in four oak species. 4. The optimum photosynthesis occurred at $25^{\circ}C$ in Q. aliens, Q. variabilis, and Q. serrate leaves, but $20^{\circ}C$ in Q. mongolica leaves. In four oak species, the net photosynthesis approached zero at about $40^{\circ}C$. 5. The dark respiration rates of leaves exhibited the following ranking of species : Q, variabilis > Q. mongolica > Q. aliens > Q. serrate. 6. The maximum productive efficiency (Pg/Rd) of leaves occurred highest in Q, serrate at $20^{\circ}C$, then in Q. mongolica at $20^{\circ}C$, then in Q, aliens at $25^{\circ}C$, and finally in Q. variabilis at $15^{\circ}C$. 7. The decrease of net photosynthesis in Q. serrate began at about -1.2 MPa, and then approached zero at -2.9 MPa of leaf water potential. The decrease of net photosynthesis began at 3% of water loss, and then approached zero at 17.5% of water loss. 8. As indicated by tissue-water relations parameters, it may be suggested that Q. aliens and Q. variabilis are more tolerant and favored on xeric forest soils than Q. mongolica and Q. serrate.

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Optimal Lactic Acid Fermentation Conditions and Quality Properties for Rubus coreanus Miquel (Bokbunja) and Chlorella Mixtures (복분자와 클로렐라 혼합물의 젖산발효 최적조건 및 품질 특성)

  • Kim, Jae-Young;Lee, Sang-Uk;Kim, Na-Hyung;Moon, Kwang-Hyun;Baek, Seung-Hwa
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.3
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    • pp.386-395
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    • 2016
  • To develop a functional fermentation food from Rubus coreanus Miquel (Bokbunja) and chlorella mixtures, optimal lactic acid fermentation conditions were established, and quality properties based on physicochemical evaluation such as chemical compositions, free sugars, organic acids, and antibacterial activities were investigated. Regarding optimal fermentation strain selection, formation of lactic acid was best in Lactobacillus plantarum among the experimental strains (10 kinds), and the optimal fermentation temperature was $37^{\circ}C$. In addition, overall acceptability in the sensory evaluation was highest in the 5% chlorella mixture sample. Therefore, quality properties of the prepared sample under the established optimal fermentation conditions were investigated. Moisture, total sugar (dry basis), crude fiber (dry basis), and pH of fermented Rubus coreanus Miquel juice (RCM) with 5% chlorella mixture (RCM-C5) were reduced by 4.90%, 14.15%, and 0.32%, respectively, as compared with non-fermented RCM. Meanwhile, crude protein, crude fat, and crude ash (dry basis) of RCM-C5 were elevated by 13.75%, 0.18%, and 0.73%, respectively, as compared with RCM. The yellowness (b value) of color values was greater in RCM-C5 compared to RCM. The free sugar and organic acid contents of RCM-C5 were elevated by 0.97% and 616.30 mg%, respectively, as compared with RCM. In addition, the gram positive bacterium Staphylococcus aureus was elevated by 5.83% while gram negative bacteria Escherichia coli and Salmonella Typhimurium were elevated by 2.94% and 4.67%, respectively, as compared with RCM. In conclusion, the quality properties of RCM and chlorella lactic acid fermentation mixtures were improved compared with the general RCM product. Consequently, it is possible to apply fermented RCM as a functional fermentation food.

Spatial Genetic Structure at a Korean Pine (Pinus koraiensis) Stand on Mt. Jumbong in Korea Based on Isozyme Studies (점봉산(點鳳山) 잣나무임분(林分)의 개체목(個體木) 공간분포(空間分布)에 따른 유전구조(遺傳構造))

  • Hong, Kyung-Nak;Kwon, Young-Jin;Chung, Jae-Min;Shin, Chang-Ho;Hong, Yong-Pyo;Kang, Bum-Yong
    • Journal of Korean Society of Forest Science
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    • v.90 no.1
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    • pp.43-54
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    • 2001
  • Genetic differentiation of populations is resulted from the environmental and the genetic effects, and the interactions between them. Whereas, the major factors influencing to the genetic differentiation within populations are the gene flow induced by seed or pollen dispersial, the microsite heterogeneity, and the density-dependent distribution of individuals. For the purpose of studying spatial genetic structure and the distribution pattern of Korean pines(Pinus koraiensis), we set up one $100{\times}100m$ plot at a Korean pine stand in Quercus mongolica community on Mt. Jumbong in Korea. To estimate the coefficient of spatial autocorrelation as Moran's index and an analogue, simple block distance, isozyme markers were analyzed in 325 Korean pines. For 11 polymorphic loci observed in 9 enzyme systems, the average percentage of polymorphic loci, the observed and expected heterozygocity were 72.2% 0.200, and 0.251, respectively. It was revealed the excess of homozygotes was observed in the plot, which suggests that here may be more number of consanguineous trees than expected. On the basis of isozyme genotypes observed in this study, 325 trees were classified into 147 groups in which the maximum number of trees for one group was 34. From the distance class of 24-32m, the genetic heterogeneity began to increase. The variation of simple block distance against the growth performance by tree height and diameter also showed the same trend at 24~32m class. According to high fixation index(F=0.204), the spatial genetic structure within a stand, the analysis of the growth performance, and the distribution patterns of identical genotypes, we inferred that the genetic structure of a Korean pine stand in Mt. Jumbong has been maintained rather density-dependent mechanism than the gene flow, such as the pollen dispersial or the heavy input of seeds following the forest gaps. The genetic patchy size was determined between 24~32m, which suggests that the selection of individuals for the ex situ conservation of Korean pine in Mt. Jumbong may be desirable to be made with the spatial distance over 37 meters between trees.

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A Study on the Sensitivity of Human Rights and the Advocacy Activities of Korean Occupational Therapists (국내 작업치료사의 인권감수성이 옹호활동에 미치는 영향)

  • Kim, Ji-Man;Hong, Ki-Hoon;Lee, Chun-Yeop;Kim, Hee-Jung
    • The Journal of Korean society of community based occupational therapy
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    • v.10 no.2
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    • pp.11-24
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    • 2020
  • Objective : The Human Rights constitute one of the basic pillars of every work where persons are involved, such is the case of the occupational therapy field. Methods : In this study we investigate the human rights sensitivity and the advocacy activities of occupational therapists. The differences according to their characteristics, the relationship and the impact of the human rights sensitivity are examined and presented. Making use of online surveys 116 subjects participated in the study. Results : The measured average of human right sensitivity is 69.00 ± 17.67 point, being them distributed according to the following subcategories: to the perception of the situation corresponds 23.25±5.62 points, to the perception of the consequences 22.75±6.54 points and for the perception of the responsibility 23±6.54 points. In all the cases have been taken in account the equal rights, the right to education in disables, the right to pursue the happiness of the elderly, the right of the disables to have personal freedom, the privacy rights and the privacy rights for mental illness people. According to the working area the Human Right sensitiveness is higher in Seoul than in the Gyeongsang province meanwhile the advocacy activities is higher in Seoul and in Gyeonggi province than in Gyeongsang province. Depending of the type of service, general hospitals and rehabilitation/nursing hospitals showed higher human rights sensitivity than other service organizations According to the working field, occupational therapy group focused in elderly showed higher Human Right sensitivity than other fields. Professionals belonging groups of clinical experience from 3 to 5 years and from 6 to 10 years showed higher advocacy activities than professionals with more than 11 years of experience. A positive correlation was showed between the human rights sensitivity and the advocacy activities. For this situation, the human rights sensitiveness was divided in sub-categories in perception of the situation, perception of the consequences and perception of the responsibility. As showed by the result of multiple regression analyses the advocacy activities of human would grow up in accordance with the increase of the human rights sensitiveness of responsibility perception. Conclusion : Due to the actual lack of information, the collection and study of basic data is fundamental for the development of practical human rights educational programs and to emphasize the role of the defense of the human rights.

Effects of specimens dimension on the flexural properties and testing reliability of dental composite resin (치과용 복합레진의 굽힘 특성과 시험 신뢰도에 미치는 시편 크기의 영향)

  • Im, Yong-Woon;Hwang, Seong-sig;Kim, Sa-hak;Lee, Hae-Hyoung
    • Korean Journal of Dental Materials
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    • v.44 no.3
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    • pp.273-280
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    • 2017
  • The aim of the present study was to investigate the effects of specimen dimension on the flexural properties and testing reliability of dental composite resin. The composite resin was prepared experimentally by mixing a resin matrix with silanated micrometer glass filler at 50 vol%. Flexural specimens with various dimension in specimen's width were fabricated by light curing using a split metal mold; $25{\times}2{\times}2mm$, $25{\times}2{\times}4mm$, $25{\times}2{\times}6mm$ in length ${\times}$ height ${\times}$ width. The flexural strength and modulus were determined according to ISO 4049 test protocol at a span length of 20 mm (normal-flexural strength; NFS). Another flexural test was conducted using mini-sized specimens ($12{\times}2{\times}2mm$, $12{\times}2{\times}4mm$, $12{\times}2{\times}6mm$) from the broken specimens at a span length of 10 mm (mini-flexural strength; MFS). Data were analyzed with ANOVA and Duncan's post-hoc test and the test reliability was evaluated by Weibull analysis. Results showed that there are generally no significant difference in flexural strength with the increase in the specimen width in NFS and MFS tests. However, the test reliability of flexural strength based on Weibull analysis was largely changed with the variables in the dimension of width and span length. The flexural modulus of NFS was increased as the dimension of specimens width increased while there was no trend in flexural modulus of MFS test. Overall results recommend that the evaluation of flexural properties and the reliability of dental composite resins should be performed with more than one test method.

Changes in Tomato Growth and Productivity under Different Night Air Temperatures (야간온도에 따른 토마토 생육 및 생산성 변화)

  • Kang, Yun-Im;Kwon, Joon-Kuk;Park, Kyoung-Sub;Choi, Gyeong-Lee;Roh, Mi-Young;Cho, Myeong-Whan;Kim, Dae-Young;Kang, Nam-Jun
    • Journal of agriculture & life science
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    • v.46 no.6
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    • pp.25-31
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    • 2012
  • This study aimed to investigate the effect of night temperature on the productivity, growth, and fruit characteristics of tomatoes and set the optimal night air temperature. Tomatoes (Solanum lycopersicum Mill. cv. Superdoterang and cv. Rapito) were grown at 5, 10, 15, and $20^{\circ}C$ during night time and ventilation temperature point during day time set $27^{\circ}C$. Depending on night air temperature, plant height, leaf area, fresh and dry weight of each organ, leaf, stem, and root were changed. Condition of high night temperature resulted in promotion of tomatoes growth with high dry weight and fresh weight. However, the dry weight under night temperature $20^{\circ}C$ decreased, especially, in 'Superdoterang'. On changes of yield, 'Rapito' showed higher productivity than 'Superdoterang' and the highest productivity point for nigh temperature is $13.32^{\circ}C$ for 'Superdoterang' and $14.25^{\circ}C$ for 'Rapito'. Although the total yield decreased from the point, the daily productivity increased with increasing night temperature, the highest point for nigh temperature is $14.45^{\circ}C$ for 'Superdoterang' and $16.46^{\circ}C$ for 'Rapito'. High temperature deceased the fruit weight and increased total soluble solid content in fruits. It is concluded that the night air temperature changes productivity of tomato and the temperatures between productivity and growth velocity have differences depending on different cultivars. scores than the other beef jerky samples (p<0.05).

A Study on stylistic features between the manuscript edition and the woodblock ediction of 『Cheonuisogameonhae』 (『천의소감언해(闡義昭鑑諺解)』 목판본과 필사본 간의 문체론적 특징 고찰)

  • Jeong, Yun Ja;Kim, Gil Dong
    • (The)Study of the Eastern Classic
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    • no.71
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    • pp.231-258
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    • 2018
  • This paper examines the differences of two different versions of "Cheonuisogameonhae" in terms of stylistics and investigates factors affecting the differences. The interpretations between the woodblock edition and the manuscript edition might be different depending on assumed range of readership, and the stylistic differences between two editions might be different depending on the possibility of extension of the reading population. Thus, this paper examines how stylistic effects are reflected in inter-relations between a translator as a speaker and readers as listeners according to speaker intentions. In Chapter 2, the stylistic differences reflected from two difference editions are examined in terms of the expression of a writer's respect, emotions, and formal consciousness to readers. The expressions of a writer's respect are more clearly emerged in the manuscript edition than in the woodblock edition. The honorific expression of a subject, '-gyeo?dsyeo', and the honorific expression of a writer, '-s?p-', are more frequently used in the manuscript edition than in the woodblock edition. In order to express positive emotions, exclamation endings are used in the manuscript edition, which shows the writer's strong emotional sympathy with readers' words and behaviors. On the other hand, in the woodblock edition, '-이' is used after names in order to treat rebellious subjects and people involved in conspiracy contemptuously by the use of informal forms. In addition, affirmative sentences in the manuscript edition and double negative sentences in the woodblock edition are used respectively, which intends to strongly emphasize a king's will and the appropriateness of the will. The writer's formal consciousness to readers are found in the way of writing names of people and places in Korean. Chinese characters are generally used two show formal consciousness; thus, names of people and places are expressed in Chinese characters in the woodblock edition. In Chapter 3, factors that made the stylistic differences between two editions are examined. The factors causing stylistic differences are examined in terms of the purpose of the interpretation, the class and range of the reading population, a writer's attitudes toward readers, and the face-to-fact situation of a writer and readers.

Family Selection on Height Growth in Open-Pollinated Progeny Trials of Pinus densiflora Using Relative Height Growth Rate (상대수고생장속도를 이용한 소나무 우수가계 선발)

  • Oh, Chang-Young;Han, Sang-Urk;Lee, Kyung-Joon;Kim, Chang-Soo;Oh, Chan-Jin;Ji, Dong-Hyun
    • Korean Journal of Breeding Science
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    • v.41 no.3
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    • pp.220-227
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    • 2009
  • This study was conducted to select superior families based on the open-pollinated (OP) progeny tests of P. densiflora. A total of 232 OP families were analyzed for relative height growth. The OP progeny test trials were established at 1 to 4 sites from 1975 to 1987. To minimize temporal and spatial variation, we applied the standardization method for family selection. In each progeny test, superior and inferior families were selected at ages of 10, 20 and 30. Relative height growth rate (RHGR), growth speed at a given time unit, was comparatively high at age of 10 with range from 0.1 to 0.6 and showed a large variation among families. However, after age 15, the RHGR was low (average 0.04) and also the variation was not significantly different among families. To reduce selection errors due to age differences (from age 23 to 35) of tests, we made the family selection after age 15 when the values of RHGR were stable. Height growth at each age was transformed to be height growth at age 35 based on the RHGR. As the results, family CB2, CB3, KW99 and KW2 were selected as superior families and KW158, KW22, KB40 and GG1 were considered as inferior ones, respectively. Rank correlations (r) between test ages and selection age 35 were high and statistically significant; r = 0.881 between age 30 and 35, 0.653 between age 20 and 35, and -0.222 between age 10 and 35.