• Journal of Mushroom
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• v.17 no.3
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• pp.119-125
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• 2019

This study aims to report the results for the analysis of the growth environment by applying smart farm technology to "Chunchu No 2" farmers in order to develop an optimal growth model for precision cultivation of bottle-grown oyster mushrooms. The temperature, humidity, carbon dioxide concentration, and illumination data were collected and analyzed using an environmental sensor installed to obtain growth environment data from the oyster mushroom cultivator. Analysis of the collected temperature data revealed that the temperature at the time of granulation was $19.5^{\circ}C$ after scraping, and the mushroom was generated and maintained at about $21^{\circ}C$ until the bottle was flipped. When the fruiting body grew and approached harvest time, mushrooms were harvested while maintaining the temperature between $14^{\circ}C$ and $18^{\circ}C$. The humidity was maintained at almost 100% during the complete growth stage. Carbon dioxide concentration gradually increased until 3 days after the beginning of cultivation, and then increased rapidly to almost 5,500 ppm. From the 6th day, carbon dioxide concentration was gradually decreased through ventilation and was maintained at 1,600 ppm during harvest. Light intensity of 8 lux was irradiated up to day 6 after seeding, and growth was then continued while periodically irradiating 4 lux light. The fruiting body characteristics of "Chunchu No 2" cultivated in the farmhouse were as follows: pileus diameter of 26.5 mm and thickness of 4.9 mm, stipe thickness of 8.9 mm, and length of 68.7 mm. The fruiting body yield was 166.8 g/850 ml, and the individual weight was 12.8 g/10 units.

• Journal of Mushroom
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• v.7 no.4
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• pp.173-181
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• 2009

These studies were investigated for effect of concentration of sodium chloride on occurrence and growth of fruitbody in oyster mushroom, Pleurotus ostreatus. When the mycelia were inoculated and cultured on the PDA plate added with the different concentrations of sodium chloride, the growth of them were not affected at the concentration of 0.5%, started to decrease at 1.0% as compared with the PDA plate without sodium chloride, and they did not grow at 5.0%. When tested at the column filled with sawdust, the tendency was similar, but the decreasing rate of mycelial growth was gentle. In sawdust bottle cultivation sprinkled with the different concentration of sodium chloride solution at the different stage, the productivity and quality of fruitbody started to decrease at 3.0% of the solution and the yield at the treatment of 10.0% solution was only 47% of that in non-treated bottles. Treated at the different stages of mushroom development, treatment just after mushroom sprout did not show any different from that just after scraping spawn from the complete grown bottles. The sprinkle treatment at this stage looked better than that of the soaking with the solution before mushroom sprout. The yield at this treatment with 10% solution showed little damage with that of 90% of non-treated bottle. Sprinkle treatment during mushroom growth stage did not affect and showed almost same to non-treated bottle except the treatment with 10.0% solution. The treatment of this stage showed better results of growing characters than the treatment after and before mushroom sprouting even 10% solution. Morphological characteristics of oyster mushroom do not distinguish to treatment of different stage and concentration of sodium chloride solution. The analyzing data of quality on underwater usually used for oyster mushroom in Inchon, Yeongjong and Kanghwa area showed over-concentration to the standard for edible water and agricultural water. But it was not confirmed whether this water quality could affect to mushroom cultivation.

• Food Science and Preservation
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• v.10 no.1
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• pp.16-22
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• 2003

This study was conducted to find out effective MA (Modified Atmosphere) gas compositions on the oyster mushroom through statistical analysis of the respiration rate and MA storage for the various packaging materials. Under the various gas compositions, the oxygen consumption rate of oyster mushroom was from 28.9 to 161.4mgO$_2$/kg$.$hr and the carbon dioxide evolution rate was from 53.4 to 166.9 mgCO$_2$/kg$.$hr at 20$^{\circ}C$. The estimated MA condition of oyster mushroom were 2.5∼4.5%O$_2$and 11.5∼l3%CO$_2$by the RSREG(Response Surface Regression). The gas compositions of MA packaging are following that 0.03mm LDPE were 1.6∼3.0%O$_2$and 3.9∼5.3%CO$_2$,0.05mm LDPE were 1.2∼1.3%O$_2$and 9.0∼11.1%CO$_2$and Nylon+PE were 0.9∼1.2%O$_2$and 33.5∼39.6%CO$_2$. The weight loss increased at 0.03mm LDPE but has the lowest value at Nylon+PE. The hardness of pileus and stipe was decreased with storage periods. The $\Delta$E-value increased with storage period and seriously changed in early storage period at 12 and 20$^{\circ}C$. In the 0.05mm LDPE, the gas compositions of packaging were similar to estimated gas compositions from the RSREG and the storage quality was superior to the other packaging materials in weight loss, hardness, and color difference at 4, 12 and 20 $^{\circ}C$.

• Journal of Mushroom
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• v.17 no.4
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• pp.205-210
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• 2019

This study was conducted to elucidate the optimal nitrogen content for each variety of oyster mushroom (Pleurotus ostreatus) by investigating the change in the characteristics of fruiting body according to the nitrogen content of the sawdust media, and to use the results as the basic data for cultivation of high quality oyster mushroom. The nitrogen content was adjusted to 1.3%, 1.6%, 1.8% and 2.3%, and at each condition, mycelial growth was observed, which showed that the nitrogen content did not affect the rate of mycelial growth and density substantially. In the investigation of the characteristics of fruiting body, at the 1.8% nitrogen content, Chunchu No.2 showed the pileus diameter of 27 mm, and the stipe diameter and length of 11 mm and 61 mm, respectively, and the measurement of its color values revealed that the brightness of the pileus and the stipe was 31 and 80, respectively, offering the best commercial value, and yielding 25 valid stipes, which was the largest number. Likewise, at the 1.8% nitrogen content, Heuktari also showed the excellent growth characteristics including the pileus diameter of 29 mm and the stipe diameter and length of 10 mm and 68 mm, respectively, and the superb color values of the brightness of 37 at the pileus and 78 at the stipe. Moreover, the number of valid stipes was also found to be the highest with 19. In terms of morphological characteristics such as the pileus diameter and color, Soltari also produced the best mushrooms at the 1.8% nitrogen content and the number of valid stipes was high with 22. In summary, irrespective of varieties of oyster mushroom, high quality products could be cultivated at the nitrogen content of 1.8%.

• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.5
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• pp.767-773
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• 1994

The dietary fiber intakes of Korean were estimated based on the data from the National Nutrition Survey in 1989. From the data, 566 foods which contain dietary fiber were selected.The intake estimation was done based on these foods. Results were summarized as followed ; Mean DF intake of nationwide population was 17.53g/day and most of these were come from vegetables. Mean DF intake of rural population was slightly lower than that of urban's. Major sources of DF were vegetables, cereals & grain products, seaweeds, fruits, fungi & mushrooms and legumens & their products in order. Vegetables were the major sources of DF which occupied 32.4% of total intake. Among them, kimchi and red pepper were the representative sources. Among cereals & grain products, rice was found to be the most important DF source occupying 12.2% of total DF. And fried noodle, loaf bread and barley were followed. Sea mustard among seaweeds, apples and persimmons among fruits, oyster mushroom and mushroom among fungi & mushrooms and soybean sprout among legumes & their products were favorable sources of DF. 653 households out of 1, 925(33.9%) were consummed 10~15g of DF per day.

• Journal of Applied Biological Chemistry
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• v.60 no.2
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• pp.149-153
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• 2017

Contamination and growth of Trichoderma, a green mold, on the oak log and wooden chip or sawdust media can severely inhibit the growth of oak mushroom. Chemicals including pesticides and antibiotics are generally not allowed for the control of green mold disease during mushroom cultivation. In this study, bacterial pathogens causing blotch disease on the oyster mushrooms were isolated and their peptide toxins were purified for the control of green mold disease. Strains of Pseudomonas tolaasii secret various peptide toxins, tolaasin and its structural analogues, having antifungal activities. These peptides have shown no effects on the growth of oak mushrooms. When the peptide toxins were applied to the green mold, Trichoderma harzianum H1, they inhibited the growth of green molds. Among the 20 strains of peptide-forming P. tolaasii, strong, moderate, and weak antifungal activities were measured from 8, 5, and 7 strains, respectively. During oak mushroom cultivation, bacterial culture supernatants containing the peptide toxins were sprayed on the aerial mycelia of green molds grown on the surface of sawdust media. The culture supernatants were able to suppress the fungal growth of green molds while no effect was observed on the mushroom growth and production. They changed the color of molds from white aerial mycelium into yellowish dried scab, representing the powerful anti-fungal and sterilization activities of peptide toxins.

• Journal of Mushroom
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• v.10 no.4
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• pp.160-166
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• 2012

The effects of fixed and variable temperatures on fruiting body formation and characters of mushrooms were investigated with normal and thinning treatment plots. In fixed temperature, as temperature was lower, period of harvest was longer as well as days for pinheading in the both of normal and thinning plots. Harvest rate were 91.0, 90.3, and 95.8% at $13^{\circ}C$, $17^{\circ}C$, and $15^{\circ}C$. In the thinning plot, qualities of mushrooms were 7.5, 8.1, and 7.7, and yields were 86.0, 105.9, 104.0g at 13, 15, and $17^{\circ}C$ respectively. In variable temperature with a variable temperature plot, condition II($17^{\circ}C$ for pin-heading(before reverting, 7-8 days)${\rightarrow}16^{\circ}C$ for extending primodia(before thinning, 4-5 days)${\rightarrow}15^{\circ}C$ for extending (after thinning)) was the best for yield which was better than other conditions by 17.4% and 4.0% and quality of mushroom was highest 8.1 among all conditions.

• Journal of Mushroom
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• v.22 no.2
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• pp.67-72
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• 2024

In this study, we investigated the microbial community of oyster mushrooms at different growth stages at the species level. Gram-positive bacteria were predominant in the presterilized medium. On the other hand, Gram-negative bacteria were predominant in the culture-completed medium, post-harvest medium, and fruiting bodies. In addition, Pseudomonas tolaasii, which is known to cause disease in mushrooms, was confirmed in the cultured medium, post-harvest medium, and fruiting bodies, and it was determined that the mycelium culture stage was contaminated, and the reason why no disease occurred was Sphingobacterium psychroaquaticum. It was confirmed that this was because the growth of Pseudomonas tolaasii was suppressed by producing a component called tolacin. As a result of confirming the diversity of microorganisms, it was confirmed that the presterilization medium contains a variety of microorganisms compared to other growth stages, and the diversity decreases in the order of culture completion medium, fruiting body, and post-harvest medium. showed a trend. As a result of microbial similarity analysis, it was confirmed that the cultured medium and the post-harvest medium showed similar microbial communities, and in the case of fruiting bodies, there were some similarities but overall differences.

• Journal of Mushroom
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• v.21 no.3
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• pp.93-100
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• 2023

In this study, research trends in mushroom science were examined using North Korean journal articles published in 1978-2023. Researchers in each field reviewed 450 papers and finally selected 429 papers, excluding 21 papers classified in different fields. The number of researchers was 872, and the number of authors per paper was 2.03. Kim Cheol-Hak published the most academic papers in the field of mushroom science in North Korea, with 12 papers. The number of research articles increased annually, from 7 in 1985, 12 in 1998, 11 in 2008, and 27 in 2020, and has especially increased rapidly since the mid-2010s. The study by mushroom type was as follows: 42 pine mushrooms (17.8 %), 25 oyster mushrooms (10.6 %), 23 Ganoderma sp. (9.8 %), 19 shiitake mushrooms (8.1 %), 17 button mushrooms (7.2 %), and 16 manna lichens (6.8 %). This study is considered meaningful in reviewing the research status and technology level in North Korea through analyzing North Korean academic journals in the field of mushroom science for the first time.

• Research in Plant Disease
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• v.9 no.2
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• pp.94-98
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• 2003

To produce antibodies against Pseudomonas tolaasii and P. agarici, lyophilized P. tolaasii and P. agarici ($5{\times}10^7$ cfu/ml) and Freund, s adjuvant were immunized into rabbits 4 times. The specificity and sensitivity of the antibodies were evaluated by immunodiffusion test and indirect enzyme-linked immunosorbent assay (id ELISA). The ${\alpha}$-P. tolaasii antibody was very specific only against P. tolaasii, while ${\alpha}$-P. agarici antibody was not specific and showed a high cross reactivity toward P. tolaasii with detection limit concentration of $2{\times}10^3$ cfu/ml. However, the cross reactivities of ${\alpha}$-P. agarici antibody toward the related species including P. reactans were very low. Our results showed that ${\alpha}$-P. tolaasii and ${\alpha}$-P. agarici antibodies against P. tolaasii and P. agarici, respectively, might be useful for rapid and simple detection of the causal agents of bacterial brown and yellow blotches in cultivated oyster mushrooms.