• Title/Summary/Keyword: Oxygen consumption rate

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A Comparative Study on the Energy Metabolism of Brains of Several Vertebtrates with Respect to Their Phyogeny (척추동물 뇌조직의 Energy 대사에 관한 계통학적 비교연구)

  • 박상윤
    • The Korean Journal of Zoology
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    • v.7 no.2
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    • pp.25-36
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    • 1964
  • The present paper deals with the comparative study on phylogenic difference in the patterns of energy metabolism of brain slices of several vertebrate species by measuring oxygen consumptionwith glucose-6-phosphate, glucose-1-phosphate, glyceraldehyde-3-phosphate or glutamate as respiratory substrate employing Warburg's manometric method, by determination of the utilization rate of glucose using glucose-1-C14 by analyzing patterns of free amino acid distribution , and by histochemical determination using glucose-1-C14 by analyzing patterns of free amino acid distribution acid distribution , and by histochemical determination of glycogen contents. 1. Glucose enhances the oxygen consumption of brain slices of animals belinging to reptile, aves and mammalia while it shows a tendency to decrease that of animals belonging to pisces and amphibia. 2. Glucose-6--phosphate increase oxygen consumption more than glucose in every species examined, while glucose-1-phosphate and glyceraldehyde-3-phosphate increase that of Rana nigromaculata only . In general m, it appears that phosphosugars are more effective as a respiratory substrate to those species which have less endogenous respiration than to those having larger endogenous respiration. 3. Similar patterns of free amino acid distribution and the relative amount are found among the species and in every species examined glutamic acid is detected in the larges amount . ${\gamma}$-Amino butyric acid, glycine, alanine and aspartic acid are found in every species. 4. Ophicephalus showed less oxygen consumption than endogenous respiration when glutamate was added to the medium. When sodium fluoride was added, the oxygen consumption was some what increased . Such phenomenon wasnot found in the frog. 5. The result of histochemcial analysis of the brain showed that glycogen was abundantly present in the fish , amphibia , and especially in the reptile and that no distinctive grains of glycogen were found in the bird and mammal . From these facts, it may be supposed that anaerobic glycolysis as energy source dominates in fish and amphibia and aerobic respiration through the oxidation of glucose dominates in bird and mamal , the reptile occupying transitional position between these two categories. The way of obtaining energy for brain activity by the oxidation of glucose supplied from the circulating blood is seemed to be first acquired by reptile and the function is completed both in aves and mammal.

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Oxygen Consumption and Blood Physiology of Olive Flounder Paralichthys olivaceus Subjected to Salinity Changes (염분 변화에 따른 넙치(Paralichthys olivaceus)의 산소 소비율과 혈액 성상)

  • Oh, Sung-Yong;Jeong, Yu Kyung;Lee, Geun Su;Kang, Pil Jun;Park, Hye Mi
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.53 no.4
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    • pp.620-627
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    • 2020
  • Oxygen consumption and blood physiology of olive flounder Paralichthys olivaceus (mean body weight 106.6±6.8 g, mean±SD) was investigated at salinities of 34.0 (control), 33.7, 33.3, 32.6, 31.3, 28.6, 23.1, 12.2 and 0.0 psu at 20.0℃, respectively. Stepwise salinity changes (34.0→33.7→33.3→32.6→31.3→28.6→23.1→12.2→0.0 psu) with an interval of 24 h for each salinity induced a significant (P<0.05) increase of oxygen consumption rate (OCR) in fish exposed from 31.3 to 0.0 psu compared to that of control fish. The maximum OCR was found in fish exposed to 23.1 psu, which was accompanied by 36.2% higher energy consumption than the control fish. Fish exposed to each salinity for 24 h induced a significant decrease of blood plasma Na+ in 0.0 psu and Cl- in 12.2 and 0.0 psu (P<0.05), and increase of plasma glutamic oxaloacetic transaminase (GOT) in 0.0 psu compared to the control fish (P<0.05). The results of this experiment show that P. olivaceus exposed to concentrations below 31.3 psu requires more energy costs to adapt to salinity changes than 34.0 psu under our experimental conditions.

Effect of Dissolved Oxygen on the Growth of Azotobacter vinelandii UWD and Production of PHBV in the Mixture of Organic Acids and Glucose (유기산 및 포도당 혼합배지에서 Azotobacter vinelandii UWD의 생장 및 PHBV 생산에 대한 용존산소의 영향)

  • 박창호
    • KSBB Journal
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    • v.13 no.6
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    • pp.675-680
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    • 1998
  • In both 7L and 20L fermentor experiments the level of dissolved oxygen (D.O) strongly affected growth and PHBV production of Azotobacter vinelandii UWD. A higher D.O. increased carbon substrate consumption rate and cell growth rate with a similar residual biomass production. However, a lower D.O. was a much better condition for PHBV production. In a 20L fermentor experiments controlled at 5% D.O. cell growth rate was about twice faster(0.555 hr-1 and 0.260 hr-1 at the acid and the glucose phase, respectively) with an equal amount(4.5 g/L) of residual biomass production. However, PHBV content in the cell(62.3 wt%) increased 17.3 times at 1% D.O.

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A Study on the Effects of Recirculated Exhaust Gas on Soot Emissions in Diesel Engines (디젤기관 매연 배출물에 미치는 재순환 배기의 영향에 관한 연구)

  • Bae, M.W.;Lim, J.K.
    • Transactions of the Korean Society of Automotive Engineers
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    • v.6 no.6
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    • pp.142-154
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    • 1998
  • The effects of recirculated exhaust gas on the characteristic of soot emissions have been investigated by using an eight-cylinder, four-stroke, direct injection and water-cooled diesel engine operating at several loads and speeds. The experiments in this study are carried out at the fixed fuel injection timing of $38^{\circ}$ BTDC regardless of experimental conditions. The intake oxygen concentration and the mean equivalence ratio calculated by the intake air flow and fuel consumption rate are used to analyze and discuss the influences of EGR rate on soot emissions. Results of this study indicate that soot emissions increase owing to the drop of intake oxygen concentration and the rise of equivalence ratio as the EGR rate increases at a given engine load and speed, especially the high load.

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Effects of Water Temperature and Salinity on the Oxygen Consumption Rate of Juvenile Spotted Sea Bass, Lateolabrax maculatus (점농어, Lateolabrax maculatus 치어의 산소 소비율에 미치는 수온과 염분의 영향)

  • Oh, Sung-Yong;Shin, Chang Hoon;Jo, Jae-Yoon;Noh, Choong Hwan;Myoung, Jung-Goo;Kim, Jong-Man
    • Korean Journal of Ichthyology
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    • v.18 no.3
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    • pp.202-208
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    • 2006
  • An experiment was conducted to investigate the effects of three water temperatures (15, 20 and $25^{\circ}C$) in combination with three salinities (0, 15 and 30 psu) on the oxygen consumption rate of juvenile spotted sea bass, Lateolabrax maculatus (mean body weight $5.5{\pm}0.3g$). The oxygen consumption rates of L. maculatus were measured in triplicate for 24 hours using a continuous flow-through respirometer. Water temperature resulted in significant differences in the mean oxygen consumption rate of L. maculatus (p<0.001), but salinity and combinations of salinity and water temperature did not have (p>0.05). The oxygen consumption increased with increasing water temperatures in all experimental salinity regimes (p<001). Mean oxygen consumption rates at 15, 20 and $25^{\circ}C$ ranged 328.8~342.3, 433.9~441.0 and 651.5~659.9 mg $O_2\;kg^{-1}\;h^{-1}$, respectively. $Q_{10}$ values did not vary with salinity, bud varied with water temperature. $Q_{10}$ values ranged 1.63~1.75 between 15 and $20^{\circ}C$, 2.24~2.26 between 20 and $25^{\circ}C$, and 1.92~1.98 over the full temperature range. The energy loss by metabolic cost increased with increasing water temperatures in all experimental salinity regimes (p<0.001) Mean energy loss rates at 15, 20 and $25^{\circ}C$ ranged 224.6~233.8, 296.3~301.2 and $444.9{\sim}450.7kJ\;kg^{-1}\;d^{-1}$, respectively. These data suggest that the culture of juvenile spotted sea bass is possible without energy loss by salinity difference in freshwater as well as seawater after salinity acclimation. Thus, this result has an application for culture management and bioenergetic model for growth of this species.

Change in Protein and Carbohydrate Contents in Diapausing and Non-diapausing Pupae of the Oriental Tobacco Budworm, Heliothis assulta Guenee (담배나방 휴면용과 비휴면용의 단백질과 탄수화물의 변화)

  • 조국호;부경생
    • Korean journal of applied entomology
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    • v.29 no.4
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    • pp.286-293
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    • 1990
  • Studies were carried out to investigate the change in protiens and carbohydrates from diapausing pupae of the Oriental tobacco budworm, Heliothis assulta Guenee. The oxygen consumption rate of non-diapausing pupae through the whole pupal period showed an U-shaped curve, while that of diapausing pupae decreased to a mean level of 20$\mu$1/g/hr. But the rate of oxygen consumption increased in diapausing pupae before their emergence. The body weight of diapausing pupae showed almost no change during 12 days after pupation. The total contents of major carbohydrates and soluble proteins were higher in diapausing pupae than those in non-diapausing pupae and the change in carbohydrate and protein contents was more dynamic in non-diapausing pupae than that of diapausing pupae. the electrophoretic band patterns of proteins were similar in both of diapausing and non-diapausing pupae. Diapausing pupae increased their haemolmph osmolarity further when they were exposed to low temperatures.

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Changes of Biomarker in Manila clam, Ruditapes philippinarum Exposed to Lead (납(Pb)에 노출된 바지락, Ruditapes philippinarum의 생물지표 변화)

  • Shin, Yun Kyung;Park, Jung Jun;Lim, Hyun Sig;Lee, Jung Sick
    • The Korean Journal of Malacology
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    • v.29 no.1
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    • pp.7-13
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    • 2013
  • This study was conducted to find out biological response of Manila clam, Ruditapes philippinarum exposed to lead (Pb). Experimental period was four weeks. Experimental groups were composed of one control condition and three lead exposure conditions (0.25, 0.50 and 1.00 mg/l). The results of the study confirmed that lead induces reduction of survival rate and oxygen consumption rate and histopathology of organ structure of the bivalve. Oxygen consumption rate was observed exposure groups lower than control decline by 25%-72%. Histological analysis of organ system illustrated expansion of hemolymph sinus, disappearance of epidermal layer and degeneration of connective tissue layer of the mantle. Also, histological degenerations as epithelial necrosis and hyperplasia of mucous cells are recognized in the gill and it was observed expansion of hemolymph sinus, disruption of epithelial layer, decrease of mucous cell and degeneration of connective tissue layer in the foot. In the digestive diverticulum, it was showed atrophy of basophilic cell and degeneration of epithelial cell in the digestive tubules, and as the concentration of lead increased the accumulation of lipofuscin increased.

The Effect of Cardiovascular on 3-Point Nonweight Bearing Ambulation with Axillary Wooden Crutch According to Age (연령에 따른 3지점 비체중지지 목발보행이 심혈관계에 미치는 영향)

  • Seo, Gyu-Weon
    • Journal of Korean Physical Therapy Science
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    • v.4 no.1
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    • pp.243-253
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    • 1997
  • The purpose of this study was to determine the effect of systolic blood pressure, heart rate and myocardial oxygen consumption on 3-point nonweight bearing ambulation with axillary wooden crutch according to age. The subjects were fifty healty volunteers(22 male, 28 female), ages from 20 to 69(mean age of 43.8 years) with no history of cardiovascular or respiratory disease, diabetes mellitus, arthritis and orthopedic disorder within the past three years. This study was carried out from May 10 to August 10, 1996. The data were analyzed by percentage, mean and standard deviation and ANOVA. The results were as follows : 1. In systolic blood pressure(SBP), there was no significantly change between before walk and after 20 m in crutch walking with age, however, there did differ significantly after 40 m and 60 m in crutch walking(p<0.01, p<0.001). 2. In heat rate(HR), there was no significantly change between before walk and after 20 m, 40 m in crutch walking with age, however, there did differ significantly after 60 m in crutch walking(p<0.05). 3. In myocardial oxygen consumption($MVO_{2}$), there was no significantly change between before walk and after 20m in crutch walking with age, however, there did differ significantly after 40 m and 60 m in crutch walking(p<0.01, p<0.001). These result showed that cardiovascular system had a great effect on the olderly when 3-point nonweight bearing ambulation with axillary wooden crutch. Therefore, when train for axillary crutch in the olderly, we needed suitably basis of walking distance.

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Energetics of the Heart Model with the Ventricu1ar Assist Device

  • Chung, Chanil-Chung;Lee, Sang-Woo;Han, Dong-Chul;Min, Byoung-Goo
    • Journal of Biomedical Engineering Research
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    • v.17 no.1
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    • pp.43-48
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    • 1996
  • We investigated the energistics of the physiological heart model by comparing predictive indexes of the myocardial oxygen consumption (MOC), such as tension-time index (R), tension-time or force-time inteual (FTI), rate-pressure product (RPP), pressure-work index, and systolic pressure-volume area (PVA) when using the electro-hydraulic left ventricular device (LVAD). We developed the model of LVAD incorporated the closed-loop cardiovascular system with a baroreceptor which can control heart rate and time-varying elastance of left and right ventricles. On considering the benefit of the LVAD, the effects of various operation modes, especially timing of assistance, were evaluated using this coupled computer model. Overall results of the computer simulation shows that our LVAD can unload the ischemic (less contractile) heart by decreasing the MU and increasing coronary flow. Because the pump ejection at the end diastolic phase of the natural heart may increase the afterload of the left ventricle, the control scheme of our LVAD must prohibit ejecting at this time. Since the increment of coronary flow is proportional to the peak aortic pressure after ventricle contraction, the LVAD must eject immediately following the closure of the aortic valve to increase oxygen availability.

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Gender Comparison of Ratings of Perceived Exertion (RPE) as a Predictor of Exercise Intensity in College Students (RPE에 의한 운동강도 예측인자의 남녀 대학생 비교)

  • Kim, Do-Yeon;Lee, Jeong-Ah
    • Journal of Life Science
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    • v.21 no.1
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    • pp.9-14
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    • 2011
  • Perceived exertion involves detection and interpretation of sensations arising from the body during physical exercise. Physiological variables such as heart rate and oxygen consumption positively correlate with ratings of perceived exertion (RPE). It is unknown whether the accuracy of predicting exercise intensity from RPE differs between men and women. Therefore, it was examined whether men or women could predict relative exercise intensity, determined by oxygen consumption, more accurately from RPE. Ten male and ten female young adult subjects aged 25.1${\pm}$3.52 yr volunteered to participate. RPE were determined by the Borg 15-category scale, and a standard Bruce treadmill protocol was used to perform graded exercise testing. There was no significant difference in slope means between males and females (p=0.501). No significant difference was observed when plotting rates of perceived exertion (RPE) vs. percentage of $VO_2$ max. The relative maximal oxygen consumptions ($VO_{2max,\;}_{rel}$) were 52.36${\pm}$7.35 ml/kg/min for males and 41.44${\pm}$6.71 ml/kg/min for females, respectively and there was a significantly high difference between the two groups in the relative $VO_{2max}$, as well as figures of 4.05${\pm}$0.36 l/min for males and 2.53${\pm}$0.39 l/min for females in the absolute $VO_{2max}$ in this study. There were no significant differences in slope, y-intercept, and standard error of estimate (SEE) between males and females. No significant difference with RPE according to exercise intensity was found between males and females. However, RPE was a useful predictor of exercise intensity in independent genders.