• 대한소아치과학회지
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• 제39권1호
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• pp.11-16
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• 2012

이 연구는 소아환자의 경구 진정요법 시 미다졸람의 비강 내 추가 투여가 심폐기능에 미치는 영향을 알아보기 위한 것으로 5 년간 삼성서울병원 소아치과에서 chloral hydrate와 hydroxyzine을 경구투여하고 $N_2O/O_2$ inhalation을 추가로 사용한 진정요법으로 치료 받은 환자 중 미다졸람을 추가투여 받은 실험군(MIDA군)과, 미다졸람을 추가 투여하지 않은 대조군 (CH-HZ군)을 각각 44명씩 선정하여 서로 비교하였다. 진정요법 중 5분마다 측정된 1) Heart rate(HR) 2) $O_2$ saturation 3) End tidal carbon dioxide concentration ($EtCO_2$), 4) Respiratory rate(RR)의 평균값을 비교하였다. 또한 동일 환자에서의 미다졸람 투여 전 후의 심폐기능지표의 변화 양상을 평가하기 위해 MIDA군 중에서 미다졸람 투여 전 후 15분 이상의 기록이 존재하는 33명을 선정하여 미다졸람 투여 전 후 15분 시점의 측정값을 각각 비교하였다. 결과는 다음과 같다. 1. Heart rate는 미다졸람을 추가 투여한 군에서 유의하게 높았으며 나머지 심폐기능 지표의 측정값들은 차이가 없었다. 두 군의 측정값들은 모두 정상범위 안에 있었다. 2. 동일 환자에서 미다졸람 투여 전 후 15분의 측정값을 비교한 결과 두 시점간에 측정된 값들은 유의한 차이가 없었다.

• 한국식품저장유통학회지
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• 제6권4호
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• pp.386-391
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• 1999

참외 CA저장시 적정 $CO_2$농도 및 $O_2$농도를 구명하기 위해 경북 성주산 참외 금싸라기 품종을 공시하여 $CO_2$농도를 5, 10, 15%로 하였고 $O_2$농도는 3, 10%로 하여 15일, 30일, 45일 동안 각각 저장하여 실험한 결과는 다음과 같았다. 참외 저장후의 부패과율은 $CO_2$농도가 높을수록 낮았으며, 중량감소율은 저장 45일까지도 일반저온저장에서 5.01%, CA저장에서는 1.0%내외로 낮았다. 과육의 경도는 저장 30일까지는 일반저온저장, CA저장 모두 저장전과 비슷한 수준으로 유지되다가 저장 45일경에는 일반저온저장에서 경도가 현저히 감소하였다. 가용성 고형물과 Vitamin C는 $CO_2$10%+O$_2$3%의 CA조건에서 저장한 참외가 다른 조건에서 저장한 참외보다 과육, 태좌부 모두 다소 높은 수준을 보였다. 저장 30일경의 과육중의 ethanol함량은 $CO_2$농도가 높을수록 많았으며 태좌부에서는 $CO_2$농도에는 큰 영향이 없었으며 $O_2$농도가 낮을수록 ethanol 함량이 많았으며 $CO_2$10%+O$_2$3%와 $CO_2$5%+O$_2$10%의 CA조건에서 ethanol축적이 비교적 적었다. Hunter L값은 $CO_2$10%+O$_2$3%의 CA조건에서 저장한 참외에서 저장초기의 상태를 가장 잘 유지되었다.

• 한국식품영양학회지
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• 제31권4호
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• pp.464-470
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• 2018

All the parts of the Cedrela sinensis A. Juss., including the seeds, roots, and leaves, have been known to exert medicinal effects. The C. sinensis and its major compound, quercetin, were previously reported to exhibit the anti-inflammatory and anti-oxidative activities. However, the hepatoprotective effects of the C. sinensis leaves against the alcohol-induced oxidative stress in the HepG2 cells have not been studied. In this study, we investigated the antioxidant activities and analyzed the flavonoid contents of the C. sinensis-leaf extract (CE). The total flavonoid contents of the CE is 1,874.5 mg/100 g dry weight (DW), while the total quercetin 3-O-rhamnoside (quercitrin) contents, which was identified as the major flavonol in the CE, is 1,456.0 mg/100 g DW. In the ethanol-stimulated HepG2 cells, the CE effectively prevented the cytotoxic effect and increased the gene expression of the antioxidant enzymes, such as the heme oxygenase-1 (HO-1) and the glutathion peroxide (GPx). The level of the reactive oxygen species (ROS) production was significantly decreased in the CE-treated HepG2 cells. In conclusion, the C. sinensis extract suppressed the alcohol-induced oxidative stress in the HepG2 cells via the induced GPx and HO-1 gene expressions. It is expected the CE positive effects will likely be attributed to the flavonoids, like the quercetin, within the CE.

• Asian-Australasian Journal of Animal Sciences
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• 제25권6호
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• pp.818-823
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• 2012

Tall fescue (Festuca arundinacea Schreb.) is an important cool season forage plant that is not well suited to extreme heat, salts, or heavy metals. To develop transgenic tall fescue plants with enhanced tolerance to abiotic stress, we introduced an alfalfa Hsp23 gene expression vector construct through Agrobacterium-mediated transformation. Integration and expression of the transgene were confirmed by polymerase chain reaction, northern blot, and western blot analyses. Under normal growth conditions, there was no significant difference in the growth of the transgenic plants and the non-transgenic controls. However, when exposed to various stresses such as salt or arsenic, transgenic plants showed a significantly lower accumulation of hydrogen peroxide and thiobarbituric acid reactive substances than control plants. The reduced accumulation of thiobarbituric acid reactive substances indicates that the transgenic plants possessed a more efficient reactive oxygen species-scavenging system. We speculate that the high levels of MsHsp23 proteins in the transgenic plants protect leaves from oxidative damage through chaperon and antioxidant activities. These results suggest that MsHsp23 confers abiotic stress tolerance in transgenic tall fescue and may be useful in developing stress tolerance in other crops.

• Archives of Pharmacal Research
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• 제27권2호
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• pp.259-264
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• 2004

The purpose of the present study was to investigate the pharmacokinetics of PEG-hemoglobin SB 1, a modified bovine hemoglobin with polyethylene glycol, after its single and multiple administration in beagle dogs. For this purpose, the analytical method of free hemoglobin in the plasma was developed and validated. Excellent linearity ($r^2$=0.999) was observed in the calibration curve data, with the limit of quantification of 0.005 g/dL. The precision and the deviation of the theoretical values for accuracy were always within $\pm$15％ in both the between-and the within-day results. The method was tested by measuring the plasma concentrations following intravenous administration to beagle dogs and was shown to be suitable for pharmacokinetic studies. In a single dose study, the plasma half-life (t$_{1}$2/) increased and the total body clearance (Cl$_{t}$) decreased with the dose (i.e., 0.017 to 0.75 gHb/kg as PEG-hemoglobin SB1) in both sexes. The volume of distribution at steady-state (Vd$_{ss}$ ) showed no difference with the dose. In contrast, the values of t$_{1}$2/, CL$_{t}$ and the area under the plasma concentration-time curve (AUC) after the multiple dose were significantly different from those of the single dose administration. The values of t$_{1}$2/ in the multiple administration were about two times higher-than that of the single dose. As a result, t$_{1}$2/ of hemoglobin after the administration of PEG-hemoglobin SB1 was about 15-30 h, indicating the PEG modification of the hemoglobin lead to a prolongation of plasma concentration of the protein. Therefore, these observations suggested that the PEG modification of hemoglobin is potentially applicable in the hemoglobin-based therapeutics.tics.

• Journal of Plant Biotechnology
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• 제41권4호
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• pp.169-179
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• 2014

세포내에는 비효소적 반응으로 활성산소류를 제거하는 소분자의 항산화 물질과 과산소와 하이드로페록사이드를 분해하는 효소들이 존재한다. 항산화 시스템은 자유라디칼과 활성산소류를 제거함으로써 산화스트레스로부터 세포 구성요소들을 보호하는 역할을 한다. 비효소적 항산화 물질은 지용성과 친수성이 있는데, 지용성 물질들은 세포막에 위치하며 과산화지질이 형성되는 반응을 억제한다. 카로테노이드와 비타민E, $CoQ_{10}$은 세포 내에서 주요 지용성 항산화 물질로써, 이들이 대량으로 축적된 주요 작물의 개발은 영양학적 가치가 높은 식품을 생산할 수 있다. 본 총설에서는 식물에서 카로테노이드와 비타민E, $CoQ_{10}$의 생합성 경로와 대사공학을 이용한 영양강화 연구 현황을 기술하였다.

• 한국축산식품학회지
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• 제44권3호
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• pp.699-709
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• 2024

Oxya chinensis sinuosa (OC) is a well-known edible insect. Several researches on the health benefits of OC consumption have been performed to date; however, their effect on eye health remains largely unknown. This study aimed to assess the protective effects of OC extracts on the oxidative stress on the retinal pigment epithelium (RPE) cells. Oxidative damage has been identified as one of the key regulatory factors in agerelated macular degeneration. H₂O₂-induced reactive oxygen species (ROS) production, a well-known oxidative stress factor, can cause cell death in retinal pigment epithelia cells. In this study, we found that three OC extracts effectively prevented H₂O₂-induced ROS production and subsequent death of ARPE-19 cells in a dose-dependent manner. In addition, the OC extracts inhibited the phosphorylation of mitogen-activated protein kinases including p38, JNK, and ERK. The OC extracts restored IκBα degradation induced by H₂O₂, indicating that OC extracts suppressed the activation of nuclear factorκB. Furthermore, the three OC extracts were shown to have antioxidant effects by upregulating the intracellular expression of key antioxidant proteins such as SOD, NQO, and HO-1. Here we demonstrated the antioxidant and anti-apoptotic effects of the OC extracts on ARPE-19, indicating their potential role in improving eye health. These results suggest that three OC extracts plays a critical role in oxidative stress-induced cell death protects in ARPE-19 cells.

• Biomolecules & Therapeutics
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• 제4권1호
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• pp.32-35
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• 1996

SKI306X is a herbal extract prepared from three herbs Clematis mandshurica, Trichosanthes kirilowii and Prunella vulgaris. It showed strong antiinflammatory actions on carrageenan-induced edema, acetic acid-induced pain, adjuvant-induced arthritis, and oxygen radical-generated reactions. In this study, the acute toxicity of SKI306X was evaluated in rats by a single oral administration. Thirty male and thirty female rats were divided into 6 groups according to the dose levels, respectively. After oral administration of SKI306X with several doses (5.0 g/kg, 3.3 g/kg, 2.2 g/kg, 1.5 g/kg, 1.0 g/kg), mortality, clinical signs, body weight, and gross findings in organs were examined. No toxic effect was shown in terms of mortality, clinical signs, body weight changes and gross findings. It is suggested the LD$_{50}$ of SKI306X would be more than 5.0 g/kg in rats.s.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2004년도 춘계학술대회 논문요약집
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• pp.300-300
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• 2004

본 연구에서는 언어과제 수행 시 일반 공기 중의 산소 농도 (21%) 환경에 비해 외부에서 고 농도 (30%)의 산소 공급이 혈중 산소 포화도(SPO$_2$), 심박동율(Heart Rate), 정답률(Accuracy), 반응속도(Reaction Time)에 어떠한 영향을 미치는지를 검증하고자 한다. 30%와 21%의 산소를 8L/min의 양으로 일정하게 공급할 수 있는 산소 공급 장치를 이용하였고, 10명의 대학생(오른손잡이, 평균나이 23.4세)을 대상으로 실험을 수행하였다. 난이도가 비슷한 두 가지 언어과제를 28문제씩 피험자에게 풀게하여 정답률과 반응속도를 계산하였다.(중략)

• 한국환경농학회지
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• 제28권4호
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• pp.371-377
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• 2009

In this study, simultaneous nitrification and denitrification (SND) from synthetic wastewater were performed to evaluate dissolved oxygen(DO) effects on chemical oxygen demand(COD) and nitrogen removal in a single membarne bio-reactor(MBR). DO levels in MBR at Run 1, 2, and 3 were 1.9~2.2, 1.3~1.6, and 0.7~1.0 mg/L, respectively. Experimental results indicated that DO had an important factor to affect COD and total nitrogen(TN) removal. SND were able to be accomplished in the continuous-aeration MBR by controlling ambient DO concentration. It is postulated that, because of the oxygen diffusion limitation, an anoxic micro-zone was formed inside the flocs where the denitrification might occur. From the results of this study, 96% of COD could be removed at DO of 0.7mg/L. At run 2 72.92% of nitrogen was removed by the mechanisms of SND (7.75mg-TN/L in effluent). In this study, SND was successfully occurred in a MBR due to high MLSS that could help to form anoxic zone inside microbial floc at bulk DO concentrations of 1.3~1.6mg/L.