• Journal of Gastric Cancer
• /
• v.17 no.4
• /
• pp.295-305
• /
• 2017

Purpose: We previously found that the histone methyltransferase suppressor of variegation, enhancer of zeste, trithorax and myeloid-nervy-deformed epidermal autoregulatory factor-1 domain-containing protein 3 (SMYD3) is a potential independent predictive factor or prognostic factor for overall survival in gastric cancer patients, but its roles seem to differ from those in other cancers. Therefore, in this study, the detailed functions of SMYD3 in cell proliferation and migration in gastric cancer were examined. Materials and Methods: SMYD3 was overexpressed or suppressed by transfection with an expression plasmid or siRNA, and a wound healing migration assay and Transwell assay were performed to detect the migration and invasion ability of gastric cancer cells. Additionally, an MTT assay and clonogenic assay were performed to evaluate cell proliferation, and a cell cycle analysis was performed by propidium iodide staining. Furthermore, the expression of genes implicated in the ataxia telangiectasia mutated (ATM) pathway and proteins involved in cell cycle regulation were detected by polymerase chain reaction and western blot analyses. Results: Compared with control cells, gastric cancer cells transfected with si-SMYD3 showed lower migration and invasion abilities (P<0.05), and the absence of SMYD3 halted cells in G2/M phase and activated the ATM pathway. Furthermore, the opposite patterns were observed when SMYD3 was elevated in normal gastric cells. Conclusions: To the best of our knowledge, this study provides the first evidence that the absence of SMYD3 could inhibit the migration, invasion, and proliferation of gastric cancer cells and halt cells in G2/M phase via the ATM-CHK2/p53-Cdc25C pathway. These findings indicated that SMYD3 plays crucial roles in the proliferation, migration, and invasion of gastric cancer cells and may be a useful therapeutic target in human gastric carcinomas.

• Journal of the Korean Society of Child Welfare
• /
• no.58
• /
• pp.231-262
• /
• 2017

South Korea has long been a culturally homogenous society, yet the increasing number of immigrants has turned the country into a multicultural society. Therefore, many empirical studies have investigated the way immigrants adapt to the Korean society and its associated factors. However, examining the overall process of migration (from family separation to reunification and cultural adaptation to the host society) that immigrant youth usually experience has been understudied. Previous empirical research and theoretical work have identified prominent factors that predict immigrant youth's mental health: experiences of family separation, living with others rather than primary caretakers during the separation, the length of residency in the host society, and family and peer support. In this respect, this exploratory and preliminary study that examined whether the experience of migration process and post-migration are related to Korean immigrant youth's mental health problems (depression, anxiety, and suicidal ideation). The result indicated that longer duration of family separation; living with relatives, siblings; and friends rather than primary caretakers during the separation; receiving less support from family and peer after migration; and a lower degree of cultural adaptation to Korean culture were associated with depression, anxiety, and suicidal ideation. Drawing upon the results, this study discussed implications for policy and practice.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.51 no.2
• /
• pp.227-234
• /
• 2015

To obtain geographical range and growth-kinetics parameters of mottled sake (Beringraja pulchra) populations in the Yellow Sea, three mark-recapture experiments were carried out. Overall, 991 tagged individuals were released, and 4.1% of them were recaptured with the mean release period of 339 d (range, 8-1,420 d) and the mean growth rate of $1.4cm\;mon^{-1}$ (female, $1.5cm\;mon^{-1}$; male $1.3cm\;mon^{-1}$). In the first experiment, 667 individuals were released at Heuksan Island from April to June, 2007-2009, and 30 individuals were recaptured mainly at the north and the north-east coasts of the island, indicating absence of migration to the south of the island. In the second experiment, 323 individuals were released at several fishing grounds scattered in the Yellow Sea in 2010-2013, and 11 individuals were recaptured at points deviated to all directions from the releasing points. As the last, one individual was released with pop-up satellite archival tag at a costal point ($34^{\circ}37.2$'N, $124^{\circ}59.3$'E) off Hong Island on May 21, 2010. The tagged individual migrated to a north-east location ($35^{\circ}50.4$'N, $126^{\circ}03.6$'E) of Eocheong Island by Aug. 25, 2010. The data archived for the three months in the tag indicated that the migration path had depths of 48-80 m and temperature of $12.6-14.4^{\circ}C$. The results indicated that mottled sake populations had a localized habitat ranges at the north of Heuksan Island and the west of Hong Island while growing at the rate of $1.4cm\;mon^{-1}$.

• Molecules and Cells
• /
• v.39 no.4
• /
• pp.345-351
• /
• 2016

Wound healing is a complex physiological process necessitating the coordinated action of various cell types, signals and microRNAs (miRNAs). However, little is known regarding the role of miRNAs in mediating this process. In the present study, we show that let-7c miRNA is decreased in heat-denatured fibroblasts and that inhibiting let-7c expression leads to the increased proliferation and migration of dermal fibroblasts, whereas the overexpression of let-7c exerts an opposite effect. Further investigation has identified heat shock protein 70 as a direct target of let-7c and has demonstrated that the expression of HSP70 in fibroblasts is negatively correlated with let-7c levels. Moreover, down-regulation of let-7c expression is accompanied by up-regulation of Bcl-2 expression and down-regulation of Bax expression, both of which are the downstream genes of HSP70. Notably, the knockdown of HSP70 by HSP70 siRNA apparently abrogates the stimulatory effect of let-7c inhibitor on heat-denatured fibroblasts proliferation and migration. Overall, we have identified let-7c as a key regulator that inhibits fibroblasts proliferation and migration during wound healing.

• Journal of Information Processing Systems
• /
• v.10 no.2
• /
• pp.193-214
• /
• 2014

Load balancing is the major benefit of any distributed system. To facilitate this advantage, task duplication and migration methodologies are employed. As this paper deals with dependent tasks (DAG), we used duplication. Task duplication reduces the overall schedule length of DAG along-with load balancing. This paper proposes a new task duplication algorithm at the time of tasks assignment on various processors. With the intention of conducting proposed algorithm performance computation; simulation has been done on the Netbeans IDE. The mesh topology of a distributed system is simulated at this juncture. For task duplication, overall schedule length of DAG is the main parameter that decides the performance of a proposed duplication algorithm. After obtaining the results we compared our performance with arbitrary task assignment, CAWF and HEFT-TD algorithms. Additionally, we also compared the complexity of the proposed algorithm with the Duplication Based Bottom Up scheduling (DBUS) and Heterogeneous Earliest Finish Time with Task Duplication (HEFT-TD).

• Journal of Chest Surgery
• /
• v.51 no.1
• /
• pp.1-7
• /
• 2018

Background: Sutureless aortic valve replacement (SU-AVR) has been developed as an alternative surgical treatment for patients with symptomatic severe aortic stenosis (AS). The aim of this study was to evaluate the clinical outcomes of SU-AVR through an assessment of hemodynamic performance and safety. Methods: From December 2014 to June 2016, a total of 12 consecutive patients with severe AS underwent SU-AVR. The endpoints were overall survival and valve-related complications (paravalvular leakage, valve thrombosis, migration, endocarditis, and permanent pacemaker implantation). The mean follow-up duration was $18.1{\pm}8.6months$. Results: The mean age of the patients was $77.1{\pm}5.8years$ and their mean Society of Thoracic Surgeons score was $9.2{\pm}17.7$. The mean cardiopulmonary bypass and aortic cross-clamp times were $94.5{\pm}37.3$ minutes and $54.9{\pm}12.5minutes$, respectively. Follow-up echocardiography showed good prosthesis function with low transvalvular pressure gradients (mean, $13.9{\pm}8.6mm\;Hg$ and peak, $27.2{\pm}15.0mm\;Hg$) at a mean of $9.9{\pm}4.2months$. No cases of primary paravalvular leakage, valve thrombosis, migration, or endocarditis were reported. A new permanent pacemaker was implanted in 1 patient (8.3%). The 1-year overall survival rate was $83.3%{\pm}10.8%$. Conclusion: Our initial experience with SU-AVR demonstrated excellent early clinical outcomes with good hemodynamic results. However, there was a high incidence of permanent pacemaker implantation compared to the rate for conventional AVR, which is a problem that should be solved.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.8
• /
• pp.3789-3795
• /
• 2014

This study aimed to analyze the expression, clinical significance of filamin A (FLNA) in renal cell carcinoma (RCC) and biological effects in a cell line by regulating FLNA expression. Immunohistochemistry and Western blotting were used to analyze FLNA protein expression in 70 cases of RCC and normal tissues to study the relationship with clinical factors. FLNA lentiviral and empty vectors were transfected into RCC to study the influence of up-regulated expression of FLNA. FLNA siRNA was transiently transfected into ACHN kidney carcinoma cells by a liposome-mediated method and protein was detected by Western blotting. The level of expression was found to be significantly lower in RCC than normal tissues (p<0.05). No correlation was noted with gender, age, tumor size or pathological types (p>0.05), but links with lymph node metastasis, clinic stage and histological grade were noted (p<0.05). Loss of FLNA expression correlated significantly with poor overall survival time by Kaplan-Meier analysis (p<0.05). Results for biological function showed that ACHN cells transfected with FLNA had a lower survival fraction, significant decrease in migration and invasion, higher cell apoptosis, higher percentage of the G0/G1 phases, and lower MMP-9 protein expression compared with ACHN cells untransfected with FLNA (p<0.05). However, renal 786-0 cells transfected with FLNA siRNA had a higher survival fraction, significant increase in migration and invasion, and higher MMP-9 protein expression compared (p<0.05). In conclusion, FLNA expression was decreased in RCC and correlated significantly with lymph node metastasis, clinic stage, histological grade and poor overall survival, suggesting that FLNA may play important roles as a a tumor suppressor in RCC by promoting degradation of MMP-9.

• Journal of Digital Convergence
• /
• v.11 no.2
• /
• pp.71-78
• /
• 2013

The purpose of the paper is to empirically investigate the role of human capital and labour market conditions in the growth process. To do so, cross-sectional data for 3062 counties across 50 states of the US. Firstly, findings from the empirical estimation suggest income convergence among US counties. Secondly, the stock of human capital appears to have the growth enhancing effect while education expenditures turn out to retard economic growth. Thirdly, it is found that the unemployment rate would have a negative association with regional growth whereas the net migration rate is likely to have a positive relationship with growth. Once the sample counties are divided into both the poor group and the rich group, finally, such main empirical results overall remain unchanged and statistically significant.

• Development and Reproduction
• /
• v.20 no.3
• /
• pp.179-185
• /
• 2016

The insulin-like growth factor (IGF) pathway is a key signal transduction pathway involved in cell proliferation, migration, and apoptosis. In dairy cows, IGF family proteins and binding receptors, including their intracellular binding partners, regulate mammary gland development. IGFs and IGF receptor interactions in mammary glands influence the early stages of mammogenesis, i.e., mammary ductal genesis until puberty. The IGF pathway includes three major components, IGFs (such as IGF-I, IGF-II, and insulin), their specific receptors, and their high-affinity binding partners (IGF binding proteins [IGFBPs]; i.e., IGFBP1-6), including specific proteases for each IGFBP. Additionally, IGFs and IGFBP interactions are critical for the bioactivities of various intracellular mechanisms, including cell proliferation, migration, and apoptosis. Notably, the interactions between IGFs and IGFBPs in the IGF pathway have been difficult to characterize during specific stages of bovine mammary gland development. In this review, we aim to describe the role of the interaction between IGFs and IGFBPs in overall mammary gland development in dairy cows.

• Journal of the Korean Dietetic Association
• /
• v.24 no.1
• /
• pp.75-91
• /
• 2018

The purpose of this study was to investigate changes in dietary life, lifestyle factors, and stress status of Chinese students who came to study abroad to South Korea. A total of 143 Chinese students were recruited from Seoul Women's University and Wonkwang University in 2013. We used SPSS 20 for statistical analyses in this study. The mean length of residence was 2.9 years for Chinese students. Most subjects aged 20~29 years immigrated to South Korea to obtain an academic degree (bachelor, master, or doctorate). We found a significant decrease in the frequency of eating fruits and vegetables among Chinese students in South Korea after migration. Further, there was a significant increase in fast food consumption and frequency of late-night snacking and eating out. In addition, their preferred beverage changed from soft drink or tea to coffee after migration. Stress level significantly (P<0.05) increased in male students compared to female students. The high stress group watched more TV when eating compared to the low stress group. In addition, there were more students who perceived their health status as bad in the high stress group. Overall results give us useful information for targeting the most vulnerable subjects and planning nutrition intervention programs.