• Title/Summary/Keyword: Out-diffusion

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Toxicity Monitoring and Assessment of Nanoparticles Using Bacteria (박테리아를 이용한 나노입자의 독성평가 및 탐지)

  • Hwang, Ee-Taek;Lee, Jung-Il;Sang, Byoung-In;Gu, Man-Bock
    • KSBB Journal
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    • v.22 no.6
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    • pp.414-420
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    • 2007
  • Nanomaterials have been applied to various fields due to their advantageous characteristics such as high surface area, rapid diffusion, high specific surface areas, reactivity in liquid or gas phase, and a size close to biomacromolecules. Up to date, increased manufacturing and frequently use of the materials, however, revoke people's concerns on their hazard impact including toxicity the materials. Many research groups have carried out different protocols to evaluate toxic effects of nanomaterilas on different organisms, and consequently, nanomaterials are known to cytotoxicity. In this paper, we reviewed some of the most reports on toxic effects of several nanoparticles specifically on bacteria. There are numbers of reports focused on antibacterial effect of nanoparticles based on bacterial cell viability. Therefore, the application of each nanomaterial should be concerned with its toxicity and its toxic effect should be evaluated in terms of concentrations and sizes of the nanomaterials used, prior to use of a nanomaterial.

Prevalence of Multi-drug Resistant Bacteria Belonging to Gram Negative Enterobacteriaceae Isolated from a Domestic Stream (국내 하천에서 분리된 그람 음성 Enterobacteriacaea의 항생제 다제내성)

  • Jang, Yejin;Song, Ki-Bong;Chung, In-Young;Kim, Hyuk;Seok, Kwang-Seol;Go, Eun Byeul;Kim, Byeori;Yoo, Yong-Jae;Rhee, Ok-Jae;Chae, Jong-Chan
    • Microbiology and Biotechnology Letters
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    • v.43 no.4
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    • pp.396-400
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    • 2015
  • Enterobacteriaceae is one of the major families responsible for public health threats. Due to the emergence of pathogens with antibiotic resistance, great concern has been raised regarding the prevalence of antibiotic resistant bacteria in natural environments. Therefore, the diversity of Gram negative Enterobacteriaceae was investigated in water samples collected from five streams in Korea using the cultivation method. Profiling of multi-drug resistance was conducted with isolates via disk diffusion assay. The results indicated that the Gram negative Enterobacteriaceae consisted of the following genus; Citrobacter, Enterobacter, Escherichia, Klebsiella, Kluyvera, Pantoea, Plesiomonas, Raoultella, Shigella and Enterobacter. These latter strains represented 49% of identified isolates. In addition, 78.3% of the identified genus exhibited resistance against more than seven out of thirteen tested antibiotics, suggesting a high prevalence of multi-drug resistant bacteria in natural environments.

An exploratory Study on Audience Experiences and Interpretations of Movercial - focused on comparative analysis with PPL (무버셜의 수용자 경험과 해석에 관한 탐색적 연구 - PPL과의 비교연구를 중심으로)

  • 이현우;김형석
    • Archives of design research
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    • v.17 no.3
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    • pp.303-312
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    • 2004
  • The purpose of this study is to activate the study on movercial. To accomplish of this, the objectives of this study are two-fold. First, this study attempts to find out how movercial are interpreted and experienced compared to product placement; and secondly, to delineate experience and interpretation in terms of the themes of movie centrality and consumption-specific relevance. This study attempted qualitative approach comparing to the criteria consisted of the themes of movie centrality and consumption-specific relevance to gain in-depth understanding of how movercial which is popped up and regarded as a newly creative format in the diffusion of internet culture and mobile communication environment is experienced and interpreted. For this aim, the moviegoer informants were classified into frequent and infrequent based on the classification method of previous product placement study of Delorme & Reid(1999) and Focus Group Interviews and In-depth Interviews were executed for understanding of how are experienced. Major implications of this study were as follows. First, prior experience of brand exposure from movercial enhanced familiarity for advertising in real-life. Second, movercial can be used to influential tools for purchasing decision with advertising and product placement. Third, informants experienced uncomfortable feeling of change and discomfort against the format of hybrid commercial message such as movercial and PPL which is penetrating into the culture and art at large.

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Interfacial disruption effect on multilayer-films/GaN : Comparative study of Pd/Ni and Ni/Pd films

  • 김종호;강희재;김차연;전용석;서재명
    • Proceedings of the Korean Vacuum Society Conference
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    • 2000.02a
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    • pp.113-113
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    • 2000
  • 직접천이형 wide band gap(3.4eV) 반도체중의 하나인 GaN를 청색 및 자외선 laser diode, 고출력 전자장비 등으로 응용하기 위해서는 낮은 접합저항을 갖는 Ohmic contact이 선행되어야 한다. 그러나 만족할만한 p-type GaN의 Ohmic contact은 아직 실현되고 있지 못하며, 이는 GaN와 접합 금속과의 구체적인 반응의 연구를 필요로 한다. 본 연구에서 앞서 Pt, Pt, Ni등의 late transition metal을 p-GaN에 접합시킨 결과 이들은 접합 당시 비교적 평탄하나 후열 처리과정에서 비교적 낮은 온도에서 기판과 열팽창계수의 차이로 인하여 평탄성을 잃어버리면서 barrier height가 증가한다는 사실을 확인하였다. 따라서 본 연구에서는 이러한 열적 불안정성을 극복하기 위하여 Ni과 Pd를 차례로 증착하고 가열하면서 interfacial reaction, film morphology, Fermi level의 움직임을 monchromatic XPS(x-ray photoelectron spectroscopy) 와 SAM(scanning Auger microscopy) 그리고 ex-situ AFM을 이용하여 밝히고자 하였다. 특히 후열처리에 의한 계면 반응에 수반되는 구성 금속원소 간의 합금현상과 금속 층의 평탄성이 밀접한 관계가 있다는 것을 확인하였다. 이러한 합금과정에서 나타나는 금속원소들의 중심 준위의 이동을 체계적으로 규명하기 위해서 Pd1-xNix와 Pd1-xGax 합금들의 표준시료를 arc melting method로 만들어 농도에 따른 금속원소들의 중심 준위의 이동을 측정하여, Pd/Ni/p-GaN 및 Ni/Pd/p-GaN 계에서 열처리 온도에 따른 interfacial reaction을 확인하였다. 그 결과 두 계가 상온에서 nitride 및 alloy를 형성하지 않고 고르게 증착되고, 열처리 온도를 40$0^{\circ}C$에서 $650^{\circ}C$까지 증가시킴에 따라 계면반응의 부산물인 metallic Ga은 증가하고 있으마 nitride는 여전히 형성되지 않는 것을 확인하였다. 증착당시 Ni이 계면에 있는 Pd/Ni/p-GaN의 경우에는 52$0^{\circ}C$까지의 열처리에 의하여 Ni과 Pd가 골고루 섞이고 그 평탄성도 유지되고 barier height의 변화도 없었다. 더 높은 $650^{\circ}C$ 가열에 의해서는 surface free energy가 작은 Ga의 활발한 편석 현상으로 인해 표면은 Ga이 풍부한 Pd-Ga의 합금층으로 덮이고, 동시에 작은 pinhole들이 발생하며 barrier height도 0.3eV 가량 증가하게 된다. 반면에 증착당시 Pd이 계면에 있는 Ni/Pd/p-GaN의 경우에는 40$0^{\circ}C$의 가열까지는 두 금속이 그들 계면에서부터 섞이나, 52$0^{\circ}C$의 가열에 의해 이미 barrier height가 0.2eV 가량 증가하기 시작하였다. 더 높은 $650^{\circ}C$가열에 의해서는 커다란 pinhole, 0.5eV 가량의 barrier height 증가, Pd clustering이 동시에 관찰되었다. 따라서 Ni과 Pd의 일함수는 물론 thermal expansion coefficient가 거의 같으며 surface free energy도 거의 일치한다는 점을 감안하면, 이렇게 뚜렷한 열적 안정성의 차이는 GaN와 contact metal과의 반응시작 온도(disruption onset temperature)의 차이에 기인함을 알 수 있었다. 즉 계면에서의 반응에 의해 편석되는 Ga에 의해 박막의 strain이 이완되면, pinhole 등의 박막결함이 줄어 들고, 이는 계면의 N의 out-diffusion을 방지하여 p-type GaN의 barrier height 증가를 막게 된다.

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Variation of Flow and Filtration Mechanisms in an Infiltration Trench Treating Highway Stormwater Runoff (고속도로 강우유출수 처리를 위한 침투도랑에서 흐름조건에 따른 여과기작 및 효율분석)

  • Guerra, Heidi B.;Yu, Jianghua;Kim, Youngchul
    • Journal of Wetlands Research
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    • v.20 no.1
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    • pp.63-71
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    • 2018
  • The particle filtration mechanisms in an infiltration trench should be varying due to the different hydraulic conditions during stormwater runoff. The understanding of these variations associated with different filtration mechanisms and their effect on the particle removal efficiency is of vital importance. Therefore, a LID (Low Impact Development) system comprising of an infiltration trench packed with gravel and woodchip was investigated during the monitoring of several independent rainfall events. A typical rainfall event was divided into separate regimes and their corresponding flow conditions as well as filtration mechanisms in the trench were analyzed. According to hydraulic conditions, it was found out that filtration changes between vertical and horizontal flows as well as between unsaturated, saturated, and partially-saturated flows. Particle separation efficiency was high (55-76%) and was mainly governed by physical straining during the unsaturated period. It was then enhanced by diffusion during the saturated period (75-95%). When the trench became partially saturated at the end of the rainfall event, the efficiency decreased which was believed to be due to the existence of a negatively charged air-water interface which limited the removal to positively charged particles.

Identification and Characterization of the Aquaporin Gene aqpA in a Filamentous Fungus Aspergillus nidulans (사상성 진균 Aspergillus nidulans에서 아쿠아포린 유전자 aqpA의 분리 및 분석)

  • Oh, Dong-Soon;Lu, Han-Yan;Han, Kap-Hoon
    • Korean Journal of Microbiology
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    • v.47 no.4
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    • pp.295-301
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    • 2011
  • Aquaporin is a water channel protein, which is classified as Major Intrinsic Protein (MIP), found in almost all organisms from bacteria to human. To date, more than 200 members of this family were identified. There are two major categories of MIP channels, orthodox aquaporins and aquaglyceroporins, which facilitate the diffusion across biological membranes of water or glycerol and other uncharged compounds, respectively. The full genome sequencing of various fungal species revealed 3 to 5 aquaporins in their genome. Although some functions of aquaporins found in yeast were characterized, however, no functional characteristics were studied so far in filamentous fungi, including Aspergillus sp. In this study, one orthodox aquaporin homolog gene, aqpA, and four aquaglyceroporin homologs, aqpB-E, in a model filamentous fungus Aspergillus nidulans were identified and the function of the aqpA gene was characterized. Knock-out of the aqpA gene didn't show any obvious phenotypic change under the osmotic stress, indicating that the function of the gene does not involved in the osmotic stress response or the function could be redundant. However, the mutant showed antifungal susceptibility resistance phenotype, suggesting that the function of the aqpA gene could be involved in sensing the antifungal substances rather than the osmotic stress response.

Effect of Fe on the High Temperature Oxidation of Ti-Al-Fe Alloys (Ti-Al-Fe계 합금의 고온산화거동에 미치는 Fe의 영향)

  • Yoon, Jang-Won;Hyun, Yong-Taek;Kim, Jeoung-Han;Yeom, Jong-Taek;Yoon, Seog-Young
    • Korean Journal of Materials Research
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    • v.21 no.7
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    • pp.357-363
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    • 2011
  • In this paper, high temperature oxidation behavior of newly developed alloys, Ti-6Al-4Fe and Ti-6Al-1Fe, is examined. To understand the effect of Fe on the air oxidation behavior of the Ti-Al-Fe alloy system, thermal oxidation tests are carried out at $700^{\circ}C$ and $800^{\circ}C$ for 96 hours. Ti-6Al-4V alloy is also prepared and tested under the same conditions for comparison with the developed alloys. The oxidation resistance of the Ti-Al-Fe alloy system is superior to that of Ti-6Al-4V alloy. Ti-6Al-4V shows the worst oxidation resistance for all test conditions. This is not a result of the addition of Fe, but rather it is due to the elimination of V, which has deleterious effects on high temperature oxidation. The oxidation of the Ti-Al-Fe alloy system follows the parabolic rate law. At $700^{\circ}C$, Fe addition does not have a noticeable influence on the amount of weight gain of all specimens. However, at $800^{\circ}C$, Ti-6Al-4Fe alloy shows remarkable degradation compared to Ti-6Al-1Fe and Ti-6Al. It is discovered that the formation of $Al_2O_3$, a diffusion resistance layer, is remarkably hindered by a relative decrease of the ${\alpha}$ volume fraction. This is because Fe addition increases the volume fraction of ${\beta}$ phase within the Ti-6Al-xFe alloy system. Activities of Al, Ti, and Fe with respect to the formation of oxide layers are calculated and analyzed to explore the oxidation mechanism.

$TiO_2$-Encapsulated EFAL-Removed Zeolite Y as a New Photocatalyst for Photodegradation of Azo Dyes in Aqueous Solution

  • ChO, Won-Je;Sook-Ja Yoon,;Yoon, Min-Joong
    • Journal of Photoscience
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    • v.12 no.1
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    • pp.17-23
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    • 2005
  • Application of a new photocatalyst has been attempted to improve the efficiency and rates of photocatalytic degradation of azo dyes by using a model dye such as Methyl Orange. As a new photocatalyst, $TiO_2$ encapsulated EFAL-removed zeolite Y ($TiO_2$ /EFAL-removed zeolite Y) has been synthesized by ion-exchange in the mixture of EFAL-removed zeolite Y with 0.05 M aqueous [$(NH_4)_2 TiO(C_2O_4)_2.H_2O$] [$TiO(C_2O_4)_2.H_2O$]. This new photocatalyst has been characterized by measuring XRD, IR and reflectance absorption spectra as well as ICP analysis, and it was found that the framework structure of $TiO_2$ /EFAL-removed zeolite Y is not changed by removing the extra-framework aluminum (EFAL) from the normal zeolite Y and the $TiO_2$ inside the photocatalyst exists in the form of $(TiO^{2+})_n$ nanoclusters. Based on the ICP analysis, the Si/Al ratio of the $TiO_2$ /EFAL-removed zeolite Y and the weight of $TiO_2$ were determined to be 23 and 0.061g in 1.0g photocatalyst, respectively. It was also found that adsorption of the azo dye in the $TiO_2$ /EFAL-removed zeolite is very effective (about 80 % of the substrate used). This efficient adsorption contributes to the synergistic photocatalytic activities of the $TiO_2$ /EFAL-removed zeolite by minimizing the required flux diffusion of the substrate. Thus, the photocatalytic reduction of methyl orange (MO) was found to be 8 times more effective in the presence of $TiO_2$ /EFAL-removed zeolite Y than in the presence of $TiO_2$ /normal zeolite Y. Furthermore, the photocatalytic reduction of MO by using 1.0 g of the $TiO_2$ /EFAL-removed zeolite Y containing 0.061g of $TiO_2$ is much faster than that carried out by using 1.0 g of Degussa P-25.

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Antimicrobial Activity of Maesil(Prunus mume) Juice against Selected Pathogenic Microorganisms (매실(Prunus mume) 착즙액의 식중독 유발균에 대한 항균 작용)

  • 이현애;남은숙;박신인
    • The Korean Journal of Food And Nutrition
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    • v.16 no.1
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    • pp.29-34
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    • 2003
  • Prunus mume has been used for the folk medicine by many old civilizations to treat food-borne diseases or enteric disorders. This study was carried out to examine the antimicrobial effect of juice from Prunus mume against pathogens such as Escherichia coli, Salmonella enteritidis, Staphylococcus aureus, Listeria monocytogenes and Bacillus cereus. The juice of Prunus mume had the strongest antimicrobial activity to Sal. enteritidis. The concentrations of Prunus mume juice for the formation of clear zone were 1% for Sai. enteritidis(15.0mm), 3% for Lis. monocytogenes(14.7mm), and 5% for Bac. cereus(14.75mm), Esc. coli(13.45mm) and Sta. aureux(11.9mm). The growth of all tested microorganisms was inhibited apparently in tryptic soy broth containing 3% and 4% Prunus mume juice. And it was found that the Prunus mume juice showed the highest antimicrobial properties, followed by Sal. enteritidis, Bac. cereus, Sta. aureus, Lis. monocytogenes, Esc. coli.

Numerical Analysis of Gas Leakage and Diffusion Behavior in Underground Combined Cycle Power Plant (지하 복합발전 플랜트 내에서의 가스 누출 및 확산 거동에 관한 수치해석 연구)

  • Bang, Joo Won;Lee, Seong Hyuk
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.4
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    • pp.118-124
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    • 2017
  • In this study, a numerical simulation was performed using commercial code Fluent(v.17.1). The underground Combined Cycle Power Plant (CCPP) was simplified to analyze the methane gas leakage with the crack size and position. In addition, extensive numerical simulations were carried out for different crack sizes from 10 mm to 20 mm. The crack position is the gas leakage, which is assumed to be near the pipe elbow and the gas turbine. A total of 4 cases were compared and analyzed. To analyze the gas leakage, the concept of the Lower Flammable Limit (LFL) was applied. The leakage distance was defined in the longitudinal direction, and the transverse direction was estimated and quantitatively analyzed. As a result, the leakage distance in the longitudinal direction varies by 52.3 % depending on the crack size at the same crack position. Moreover, the maximum difference was 34.8 % according to the crack position when the crack sizes are identical. As jet flow impacts on the obstacle and changes its direction, the recirculation flows are formed. These results are expected to provide useful data to optimize the location and number of gas detections in confined spaces, such as underground CCPP.