• Molecules and Cells
• /
• 제22권2호
• /
• pp.182-188
• /
• 2006

Dlx5 and Osx are master regulatory proteins essential for initiating the cascade leading to osteoblast differentiation in mammals, but the mechanism of osteoblast-specific expression is not fully understood. DNA methylation at CpG sequences is involved in tissue and cell type-specific gene expression. We investigated the methylation status of Dlx5 and Osx in osteogenic and nonosteogenic cell lines by methylationspecific PCR (MSP). The CpG dinucleotides of the Dlx5 and Osx promoter regions were unmethylated in osteogenic cell lines transcribing these genes but methylated in nonosteogenic cell lines. Treatment of C2C12 cells with 5-AzadC induced dose- and timedependent expression of Dlx5 and Osx mRNA by demethylating the corresponding promoters. Furthermore the mRNAs for the osteoblast markers ALP and OC, which were undetectable in untreated cells, gradually increased after 5-AzadC treatment. In addition, BMP-2 stimulation induced Dlx5 expression by hypomethylating its promoter. These findings suggest that DNA methylation plays an important role in cell type-specific expression of Dlx5 and Osx.

• 한방재활의학과학회지
• /
• 제30권4호
• /
• pp.1-16
• /
• 2020

Objectives The main purpose of this study was to evaluate the bone healing effect of Pahyeolsandong-tang (PHT)(Poxiesanteng-tang) extract in tibia fracture-induced mice. Methods PHT was extracted using a solution of 35% ethanol in 60℃ for 8 hours. Mice were randomly divided into 4 groups (normal, control, PHT 50 and PHT 100). Mice of experimental groups were medicated with PHT 50 or 100 mg/kg for 7 to 21 days. To clarify the effect of bone fracture healing, relative messenger RNA (mRNA) expressions of osteocalcin (OCN), runt-related transcription factor 2 (Runx2), osterix (OSX), Sox9, collagen type II alpha 1 chain (Col2a1), receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG) were examined. Results In in vitro experiment, relative mRNA expression of OCN, Runx2, Col2a1 was significantly increased in PHT treated group to compare with control differentiation group. In in vivo experiment, relative mRNA expression of OCN, Runx2, OSX, Sox9, Col2a1, RANKL, OPG was significantly increased in PHT treated group. Conclusions This study showed that PHT accelerates bone fracture healing through the activation of osteoclasts and osteoblasts. It was showed that PHT significantly promotes osteoblasts differentiation by osteoblast differentiation markers such as OCN, Runx2, Col1a2. Also it was investigated that PHT had stimulatory effect on osteoblasts function through enhancing OCN, Runx2, OSX, Sox9, Col2a1 and, osteoclasts function through enhancing RANKL and OPG markers. PHT effectively promotes bone fracture healing process through activation of osteoblasts and osteoclasts.

• 한국콘텐츠학회:학술대회논문집
• /
• 한국콘텐츠학회 2005년도 춘계 종합학술대회 논문집
• /
• pp.165-171
• /
• 2005

MPEG II 비디오 스트림과 오디오 AC-3 스트림, 자막 스트림에 지역 코드, 복제 방지 메뉴 등을 프로그램 하여 하나의 스트림으로 만들고, 시나리오에 따라 속성, 명령 및 작동을 부여하여 최종 디스크 이미지(DVD)를 만드는 과정을 오소링(Authoring)이라고 한다. 이러한 오소링 (Authoring) 툴들은 다양하게 출시되어 있는데, 간단한 타이틀을 만드는 보급형부터 전문적인 영화 타이틀을 만드는 고급형까지 용도에 따라 선택하여 쓸 수 있다. 본 논문에서는 일반적인 IEEE 1394포트를 이용하여 영상과 음향을 컴퓨터로 캡처(Capture)해서 DVD를 만드는 오소링(Authoring) 과정을 일반인들이 Desktop PC로써 가장 많이 사용하고 있는 Window체계의 PC와 OSX 체계의 Macintosh를 중심으로 비교, 분석하고자 한다.

• 한국콘텐츠학회논문지
• /
• 제5권5호
• /
• pp.145-151
• /
• 2005

MPEG II 비디오 스트림과 오디오 AC-3 스트림, 자막 스트림에 지역 코든 복제 방지 메뉴 등을 프로그램 하여 하나의 스트림으로 만들고, 시나리오에 따라 속성, 명령 및 작동을 부여하여 최종 디스크 이미지(DVD)를 만드는 과정을 오소링(Authoring)이라고 한다. 이러한 오소링(Authoring) 툴들은 다양하게 출시되어 있는데, 간단한 타이틀을 만드는 보급형부터 전문적인 영화 타이틀을 만드는 고급형까지 용도에 따라 선택하여 쓸 수 있다. 본 논문에서는 일반적인 IEEE 1394포트를 이용하여 영상과 음향을 컴퓨터로 캡처(Capture)해서 DVD를 만드는 오소링(Authoring) 과정을 일반인들이 Desktop PC로써 가장 많이 사용하고 있는 Window체계의 PC와 OSX 체계의 Macintosh를 중심으로 비교, 분석하고자 한다.

• 한방재활의학과학회지
• /
• 제30권1호
• /
• pp.13-29
• /
• 2020

Objectives This study was performed to decide the bone union effect of Hwaweo-jeon on tibia fractured mice. Methods In this study, laboratory experiments were implemented by the stage of in vitro and in vivo. In in vitro, MC3T3-E1 cells were treated with various concentration of Hwaweo-jeon extract (HWJ). To investigate effect of HWJ for osteoblast, relative mRNA expression of 5 substances (alkaline phosphatase [ALP], runt-related transcription factor 2 [Runx2], osteocalcin [OCN], osterix [OSX] and collagen type II alpha 1 chain [Col2a1]) was used as a marker of osteogenesis. In order to determine HWJ's effect for fracture healing, relative gene expression level of ALP, Runx2, OCN, OSX and Col2a1 were used to find out the influence to osteoblast. Furthermore, receptor activator of nuclear factor kappa-B ligand and osteoprotegerin relative mRNA expression were used to estimate the impact to osteoclast. Also, X-ray was used for the purpose of identifying bone union in tibia-fracture mouse model. Results In in vitro experiment, most part of relative mRNA expression were increased compared to control group. In in vivo and in vitro experiment, HWJ induced osteoblast activitation by verifying relative mRNA expression of 5 substances. And in vivo experiment, we can also identify that HWJ triggered osteoclast activation during early stage of tibia fracture. Furthermore, X-ray pictures show noticeable recovery of tibia fracture. Conclusions HWJ extract promotes bone union by facilitating the osteoblast. But, HWJ may occur liver & kidney toxicity over specific concentration. Therefore, when HWJ is applied to human body, doctors have to follow up the liver function test & renal function test of patient.

• 한국콘텐츠학회논문지
• /
• 제19권12호
• /
• pp.164-172
• /
• 2019

본 논문에서는 실내 환경에서 시각정보를 기반으로 출발지점에서 경유지를 거쳐 목표지점으로 최적의 경로를 찾아 자율 주행하는 바퀴달린 로봇을 구현한다. 로봇은 출발지점에서 경유지를 거쳐 목표지점으로의 최적의 경로를 딥강화학습으로 얻을 수 있다. 그러나 로봇이 구해진 경로로 자율 주행을 할 때 표면의 굴곡과 이물질 등의 외부적 요인으로 목적지까지 정확하게 주행하지 못하는 경우가 발생한다. 이에 본 연구는 카메라만 장착한 로봇이 외부 요인으로 인해 최적의 경로를 이탈할 경우 이를 인지하도록 한다. 이 인지를 토대로 로봇이 스스로 경로를 보정하고 계획된 경유지와 최종 목적지점에 도달할 수 있게 하는 알고리즘을 제안한다. 본 연구를 위해 파이캠을 탑재한 라즈베리파이와 아두이노로 제어하는 바퀴식 자율 주행 로봇이 제작되었다. 로봇은 실내환경에서 OSX 환경의 서버와 실시간 연동하면서 계획된 최적의 경로로 시험주행을 완료하였다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제33권4호
• /
• pp.381-385
• /
• 2007

Bone is a dynamic organ that bone remodeling occurs throughout life and involves the process in which the bone matrix is broken down through resorption by osteoclasts and then built back again through bone formation by osteoblasts. Usually these two processes balance each other and a stable level of bone mass is maintained. We here discuss transcription factors involved in regulating the osteoblast differentiation pathway. Runx2 is a transcription factor which is essential in skeletal development by regulating osteoblast differentiation and chondrocyte maturation. Its companion subunit, Cbf${\beta}$ is needed for an early step in osteoblast differentiation pathway. Whereas Osterix(Osx) is a new identified osteoblast-specific transcription factor which is required for the differentiation of preosteoblasts into more mature and functional osteoblasts. We also discuss other transcription factors, Msx1 and 2, Dlx5 and 6, Twist, and Sp3 that affect skeletal patterning and development. Understanding the characteristics of mice in which these transcription factors are inactivated should help define their role in bone physiology and pathology of bone defects.

• Journal of Korean Dental Science
• /
• 제9권1호
• /
• pp.9-18
• /
• 2016

Purpose: Wnt signaling plays an essential role in the dental epithelium and mesenchyme during tooth morphogenesis. Deletion of the Wntless (Wls) gene in odontoblasts appears to reduce canonical Wnt activity, leading to inhibition of odontoblast maturation. However, it remains unclear if autonomous Wnt ligands are necessary for differentiation of dental pulp cells into odontoblast-like cells to induce reparative dentinogenesis, one of well-known feature of pulp repair to form tertiary dentin. Materials and Methods: To analyze the autonomous role of Wls for differentiation of dental pulp cells into odontoblast-like cells, we used primary dental pulp cells from unerupted molars of Wls-floxed allele mouse after infection with adenovirus for Cre recombinase expression to knockout the floxed Wls gene or control GFP expression. The differentiation of dental pulp cells into odontoblast-like cells was analyzed by quantitative real-time polymerase chain reaction. Result: Proliferation rate was significantly decreased in dental pulp cells with Cre expression for Wls knockout. The expression levels of Osterix (Osx), runt-related transcription factor 2 (Runx2), and nuclear factor I-C (Nfic) were all significantly decreased by 0.3-fold, 0.2-fold, and 0.3-fold respectively in dental pulp cells with Wls knockout. In addition, the expression levels of Bsp, Col1a1, Opn, and Alpl were significantly decreased by 0.7-fold, 0.3-fold, 0.8-fold, and 0.6-fold respectively in dental pulp cells with Wls knockout. Conclusion: Wnt ligands produced autonomously are necessary for proper proliferation and odontoblastic differentiation of mouse dental pulp cells toward further tertiary dentinogenesis.

• Tissue Engineering and Regenerative Medicine
• /
• 제15권6호
• /
• pp.793-801
• /
• 2018

BACKGROUND: The aim of this study was to evaluate the combined effect of low-level laser treatment (LLLT) and recombinant human bone morphological protein-2 (rhBMP-2) applied to hypoxic-cultured MC3T3-E1 osteoblastic cells and to determine possible signaling pathways underlying differentiation and mineralization of osteoblasts under hypoxia. METHODS: MC3T3-E1 cells were cultured under 1% oxygen tension for 72 h. Cell cultures were divided into four groups: normoxia control, low-level laser (LLL) alone, rhBMP-2 combined with LLLT, and rhBMP-2 under hypoxia. Laser irradiation was applied at 0, 24, and 48 h. Cells were treated with rhBMP-2 at 50 ng/mL. Alkaline phosphatase activity was measured at 3, 7, and 14 days to evaluate osteoblastic differentiation. Cell mineralization was determined with Alizarin red S staining at 7 and 14 days. Western blot assays were performed to evaluate whether p38/protein kinase D (PKD) signaling was involved. RESULTS: The results indicate that LLLT and rhBMP-2 synergistically increased alkaline phosphatase (ALP) activity and mineralization. Western blot analyses showed that expression of type I collagen, runt-related transcription factor 2 (RUNX2), and Osterix (Osx), increased and expression of hypoxia-inducible factor 1-alpha ($HIF-1{\alpha}$), decreased more in the LLLT and rhBMP-2 combined group than in the rhBMP-2 or LLL alone groups. Moreover, LLLT and rhBMP-2 stimulated p38 phosphorylation and rhBMP-2 and LLLT increased Prkd1 phosphorylation. CONCLUSION: Combined treatment with rhBMP-2 and LLL induced differentiation and mineralization of hypoxic-cultured MC3T3-E1 osteoblasts by activating p38/PKD signaling in vitro.

• 한국HCI학회:학술대회논문집
• /
• 한국HCI학회 2008년도 학술대회 2부
• /
• pp.30-35
• /
• 2008

컴퓨터 OS 환경의 기술적 발달과 더불어 GUI 에서도 많은 변화를 가져왔다. MS의 Vista OS나 애플사의 OSX Leopard 등의 여러 PC상의 OS가 기존의 기능 중심적 환경에서 벗어나 사용자들에게 감성적인 부분에 대해 호소하기 위한 디자인 요소를 부각시키는 단계로 접어들었다. 특히 Mac OS, Linux OS 환경의 지속적 확산 및 Web 기반 OS의 개발, 다양한 OS Skin의 개발 등에 따라 일률적이던 OS 환경은 변화의 시기를 맞이하고 있다. 이에 따라 PC OS의 GUI 디자인이 사용자 감성에 미치는 영향에 대한 객관적 평가를 통해 향후 PC OS의 GUI개발을 위한 기초자료 수집에 연구의 목적이 있다. 이를 위하여 감성 공학적 분석방법인 의미분별 척도법(Semantic Differential Method)을 통해 사용자 감성 선호도를 분석하였다. 감성어휘 수집을 위한 1차 실험으로 47개 이상의 기초 감성어휘를 추출 한 후 어휘의 분포도 및 어휘 유사성에 근거한 적절성 평가로 실험에 사용될 20개의 어휘를 최종적으로 추출하였다. 선정된 어휘를 척도등급반의어와 평가등급 반의어, 정감등급반의어의 세 원칙에 따라 질문서로 작성하고 이에 따른 사용자 감성 반응을 측정하였다. 2차 실험은 샘플 이미지에 대한 선호도 조사 그리고 각 샘플별 5점 척도를 이용한 평가 수행으로 이루어졌다. 선호도조사를 통해 사용자는 '선명하다', '쉽다', '안전하다', '안정적이다'로 어휘화 될 수 있는 GUI 디자인을 선호하는 것으로 나타났다. 또한 선명하다는 이미지는 안정감을 느끼게 하며 쉰다고 느끼는 이미지는 안전함과 연관성을 확인할 수 있었다. 이러한 사용자의 PC OS에 대한 감성연구 결과는 향후 PC OS의 GUI 디자인을 위한 기초자료로 활용 될 수 있을 것이다.