Browse > Article
http://dx.doi.org/10.18325/jkmr.2020.30.1.13

Affirmative Effect of Hwaweo-jeon (Huayu-jian) in Osteoblast Cells and Tibia Fracture-induced Mice  

Lee, Soo-Hwan (Department of Oriental Medicine, Sangji University General Graduate School)
Parichuk, Kira (Department of Pharmacology, College of Korean Medicine, Sangji University)
Cha, Yun-yeop (Department of Rehabilitation Medicine of Korean Medicine, Sangji University Korean Medicine Hospital)
Publication Information
Journal of Korean Medicine Rehabilitation / v.30, no.1, 2020 , pp. 13-29 More about this Journal
Abstract
Objectives This study was performed to decide the bone union effect of Hwaweo-jeon on tibia fractured mice. Methods In this study, laboratory experiments were implemented by the stage of in vitro and in vivo. In in vitro, MC3T3-E1 cells were treated with various concentration of Hwaweo-jeon extract (HWJ). To investigate effect of HWJ for osteoblast, relative mRNA expression of 5 substances (alkaline phosphatase [ALP], runt-related transcription factor 2 [Runx2], osteocalcin [OCN], osterix [OSX] and collagen type II alpha 1 chain [Col2a1]) was used as a marker of osteogenesis. In order to determine HWJ's effect for fracture healing, relative gene expression level of ALP, Runx2, OCN, OSX and Col2a1 were used to find out the influence to osteoblast. Furthermore, receptor activator of nuclear factor kappa-B ligand and osteoprotegerin relative mRNA expression were used to estimate the impact to osteoclast. Also, X-ray was used for the purpose of identifying bone union in tibia-fracture mouse model. Results In in vitro experiment, most part of relative mRNA expression were increased compared to control group. In in vivo and in vitro experiment, HWJ induced osteoblast activitation by verifying relative mRNA expression of 5 substances. And in vivo experiment, we can also identify that HWJ triggered osteoclast activation during early stage of tibia fracture. Furthermore, X-ray pictures show noticeable recovery of tibia fracture. Conclusions HWJ extract promotes bone union by facilitating the osteoblast. But, HWJ may occur liver & kidney toxicity over specific concentration. Therefore, when HWJ is applied to human body, doctors have to follow up the liver function test & renal function test of patient.
Keywords
Hwaweo-jeon (Huayu-jian); Tibia fractured mice; Osteoblasts; Osteoclasts; Bone union; mRNA expression;
Citations & Related Records
Times Cited By KSCI : 10  (Citation Analysis)
연도 인용수 순위
1 Chung SY, Choi HJ, Chung MW, Ahn MR, Yoo TM, Rheu HM, Yang JS. Effects of safflower-seed on the fracture healing in rat tibia. Yakhak Heoji. 1999;43(4):526-34.
2 Seo HJ. Effects of safflower seed powder and its fraction in bone tissue in rib-fractured rats during the recovery. Journal of Nutrition and Health. 2000;33(4):411-20.
3 Yoon JW, Kim SJ. The effect of Gyejibokryunghwan on fracture union in tibia-fractured rats. Journal of Korean Medicine Rehabilitation. 2019;29(4):1-14.   DOI
4 The Society of Korean Medicine Rehabilitation. korean rehabilitation medicine. 4th ed. Paju:Koonja Publishing. 2015:15, 206-10.
5 Bonnarens F, Einhorn TA. Production of a standard closed fracture in laboratory animal bone. J Orthop Res. 1984;2(1):97-101.   DOI
6 Hole JW. Human anatomy and physiology. Dubuque:W.C. Brown Publishers. 1993:170-227.
7 Wang D. Oedaebiyo. 1st ed. Seoul:Sungbosa. 1975:749-50.
8 Son S. Bigeubcheongeumyobang. 1st ed. Beijing: Inminwiseng Publishing Company. 1982:454-5.
9 Jin S. Taepyunghyeminhwajegukbang. 1st ed. Peking:Si Pung publishing company. 1976:220-7.
10 Jo G. Seongjechongrok. 1st ed. Seoul:Yeogang Publishing Company. 1987:460-4.
11 Bae KJ, Jeong JW, Jung MY, Kim SJ. Reviewing research on the treatment and study of fracture in Korean journals objective - focus on domestic thesis. Journal of Korean Medicine Rehabilitation. 2015;25(3):27-36.   DOI
12 Oh G. Uijonggeumgam. 1st ed. Seoul:Bubin Publishing Company. 2006:1250.
13 Jeon SC. Sanggwaboyo. 1st ed. Seoul:Iljung Publishing Company. 1992:176.
14 Kang HS, Hong SH, Kang CH. Musculoskeletal Radiology. 1st ed. Seoul:Panmuneducation. 2015: 267-81.
15 Jeon DH. Healing effect of danggwisu-san (Dangguixu-san) on femur fractured mice. Daejeon University. 2018:32.
16 The Society of Pathology in Korean Medicine. Pathology in Korean Medicine. 2nd ed. Seoul:Omdbook. 2009:335-6.
17 Lee C. Euihakipmun. 1st ed. Seoul:Bubinbooks. 2009:2034.
18 Heo J. Dongeuibogam. 1st ed. Seoul:Bubinbooks. 2005:2839.
19 Hwang DY. Bangyakhappyun. 1st ed. Seoul:Younglimsa. 2011:322.
20 Chang HK, Kim HS, Kim S J , Kim SY, Park J H, B ae HJ, Shin WC, Yoon YJ, Lee HS, Chung WS. Korean medical treatment including phototherapy after hip fracture surgery: three case reports. Journal of Korean Medicine Rehabilitation. 2018;28(4):113-24.
21 Hong JR, Kim YJ , Kim J S, Lee J R, Kim TG, Yoo DH, Choi IS, Lee SW. 4 cases of patients that improved from traffic accident which caused lumbar spine compression fracture by complex Korean medical admission treatment: retrospective case study. Journal of Korean Medicine Rehabilitation. 2017;27(4):163-70.   DOI
22 Lee KS, Lee GM, Yeom SC. Clinical study on the case of lumbar compression fracture with traditional Korean medicine, especially the SAAM acunpture, Carthami-Flos herbal acupuncture. Korean J Oriental Physiology & Pathology. 2004;18(4):1228-31.
23 The Textbook Compilation Committee of the Histology. Human histology. 6th ed. Seoul:komoonsa. 2011. 104-5.
24 Keum DH, Kim SS. Healing effect of pyrite on tibia fractured rats. Journal of Korean Medicine Rehabilitation. 2002;12(2):51-68.
25 Kim YK, You H, Lim HC, Lee JS, Jung UW, Lee YK, Choi SH. Development and evaluation of synthetic bone graft materials for periodontal tissue regeneration and carriers. Implantology. 2014;19(4):246-59.
26 Kee YB, Kim DH, Kang DH, Kim SJ, Choi JB. Effects of Yukmijihwang-tang (Liuweidihuang-tang) and cervi pantotrichum cornu pharmacopuncture on fracture healing in diabetic rats. Journal of Korean Medicine Rehabilitation. 2012;22(3):49-63.
27 Ahn HL, Shin MS, Kim SJ, Choi BS. Effects of neutral eohyeol (Yuxue) herbal acupuncture and dangkisoo-san (Dangguixu-san) on fracture healing in the early stage in rats. Journal of Oriental Rehabilitation Medicine. 2007;17(1):1-16.
28 Hong TG, Oh MS, Song TW, Kim KS. Healing effect of sintongchugoetang water extract on tibia fractured rats. Daejeon University, Institute of Korean Medicine. 1999;8(1):727-38.
29 Keum DH, Kim SS. Healing effect of bokwonhwalhyul-tang on tibia fractured rats. The Journal of the Korea Institute of Oriental Medical Informatics. 2002;8(1):46-66.
30 Yeom IH. Gamigungguitang and Gamigungguitang GaNokyong water extract on tibia fractured rats. Daejeon University, Institute of Korean Medicine. 1999;8(1):675-87.
31 Longo DL, Fauci A, Kasper D, Hauser S, Jameson JJ, Loscalzo J. Harrison's principles of internal medicine. 17th ed. Seoul:Mipmedical. 2010:2318.
32 Kim HJ, Kim JH, Son ES, Lee JM, Park HR. Neuroprotective effect of extracts from root bark of morus alba on glutamate-induced cytotoxicity in neuronal cells. Journal of Life Science. 2009;19(7):963-7.   DOI
33 Allen MJ. Biochemical markers of bone metabolism in animals: uses and limitations. Veterinary Clinical Pathology. 2003;32(3):101-13.   DOI
34 Korean Society for Laboratory Medicine. Laboratory Medicine. 4th ed. Seoul:E-public. 2013:115.
35 Association of Korean Medicine Professors for Kidney Medicine. Kidney medicine in Korean medicine. 1st ed. Seoul:Koonja. 2013:212.
36 Calvo MS, Eyre DR, Gundburg CM. Molecular basis and clinical application of biological markers of bone turnover. Endocrine Reviews. 1996:17(4):333-68.   DOI
37 Jin H, Wang B, Li J, Xie W, Mao Q, Li S, Dong F, Sun Y, Ke H. Z, Babij P, Tong P, Chen D. Anti-DKK1 antibody promotes bone fracture healing through activation of ${\beta}$-catenin signaling. Bone. 2015;71:63-75.   DOI
38 Association of Korean Medicine Professors for Liver Medicine. Liver medicine in Korean medicine. 5th ed. Seoul:Nado Publishing Co. 2013:243-4.
39 Ducy P, Zhang R, Geoffroy V, Ridall AL, Karsenty G. Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell. 1997;89(5):747-54.   DOI
40 Yoshikawa M, Ogata A. Expression of the osteoblastic marker in human alveolar bone cells spheroid. Nihon Shishubyo Gakkai kaishi. 2006;48(4):276-84.   DOI
41 Szabova L, Yamada SS, Wimer H, Chrysovergis K, Ingvarsen S, Behrendt N, Engelholm LH, Holmbeck K. MT1-MMP and type II collagen specify skeletal stem cells a nd t heir b one a nd c artilage p rogeny. Journal of Bone and Mineral Research. 2009;24(11): 1905-16.   DOI
42 Choi HS. Recent update on RANKL inhibitor. The Korean Journal of Medicine. 2018;96(3):252-9.   DOI
43 Lee JH, Lee MS, Chae SU, Kim HY, Moon SY, Jeon BH, Cho HJ. Effect of water extract of aloe in RANKLinduced osteoclast differentiation. Korean J Oriental Physiology & Pathology. 2011;25(6):1008-13.
44 Park SY, Suh KT, Ryu CH, Woo SH, L ee J S, Kim SG. Alterations in serum levels of receptor activator of nuclear $factor-{\kappa}B$ligand and osteoprotegerin in patients with head injury and fracture. Journal of the Korean Fracture Society. 2008;21(2):145-50.   DOI
45 Yoo JH, Kim HI, Kim GW, Koo BS. A case of delirium with traumatic subdural hemorrhage patient healed by Hwaeo-jeon. Journal of Oriental Neuropsychiatry. 2005;16(2):190-9.
46 Li YC, Oh MS. Effects of Joagjuhwan (JGH, 左歸丸) extract on changes of anti-oxidation, anti-inflammatory in RAW 264.7 cells and on factors related with bone metabolism in skull fractured rat. Journal of Korean Medicine Rehabilitation. 2016;26(3):31-2.   DOI
47 Ronald M. Practical fracture treatment. 2nd ed. Seoul: Cheil Company. 1993:28-35.
48 Kim YH. Cheonggangeuigam. 1st ed. Seoul:Sungbosa. 2001:305, 379.
49 Kim HJ, Cho AR, Kim CH. A clinical case report of tinnitus after head trauma. The Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology. 2011;24(2):111-8.