• Journal of Oral Medicine and Pain
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• 제9권1호
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• pp.11-22
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• 1984

The authors administered KCN, NaF,AS2O3 orally to rabbits and caused acute and chronic poisoning, then studied the teeth, jaw bones, and other oral tissues histopathologically. The results were as follows : 1. There was no significant difference between acute poisoned group by NaF and control group. But, vasodilatation in the connective tissues, esepcially marginal area of jaw bone, atrophy and destrution of glandualr cells was observed. 2. Chronic poisoned group by NaF showed degeneration and thicking of subcutanece fibrosis ective tissues, atrophy and degeneration of subcutaneous connective tissues, atrophy and degeneration of muscle fibers, vasodilation of subcutaneous in bone cavities(lacunae), and degeneration of odotlblasts in pulp tissue. 3. Acute poisoned group by KCN showed almost similar appearances as control group, and chronic poisoned group showed hyperplasia of baal layer in epitheilium, degeneration of subcutaneous connective tissues, vasodilation and huperemia, severe hemorrhage of marginal area of jaw bone. hyperplasia of salivary gland ducts, but normal arrangement of muscle fibers and narrow bone carity(lacunae) due to active osteoblastic action, osteodentin were observed. 4. Acute poisoned group by AS2O3 showed degeneration of basal cell, atrophy of blood vessels in palatal muscosa. Chronic poisoned group showed irregular cell arrangement and degeneration, reduction of capillaries in palatal mucosa. Osteoclasts in jaw bone were observed. 5. In Masson's Trichrome and Van Gieson Staining, chronic poisoned group by NaF showed thicking and loosening of subcutaneous connective tissues. Hyperplasia of intermuscular connective tissue was observed in chronic poisoning by KCN and NaF. In PAS staining, negative reation in outer layer of palatalmucosa, positive reaction in keratin layer and mild reaction of basal layer in palate and tongue mucosa was observed.

• Restorative Dentistry and Endodontics
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• 제22권2호
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• pp.710-719
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• 1997

Aucubin, an iridoid glucoside, which is isolated from Aucuba japonica, has some biological effects. This study was to investigate the effect of aucubin on the remainig pulp tissues after pulpotomy. Mongrel dog's coronal pulps were mechanically exposed with a sterile round bur and excised with sterile sharp excarvator. After bleeding was controlled, in control group, $Ca(OH)_2$ powder was applied on the remaining pulps and the cavities were sealed with Z.O.E. cement. In experimental group 1, mixed powder with $Ca(OH)_2$ and aucubin(l : 1 by weight) was applied on the pulpotomized pulp surfaces. After the cavities were covered with sterile aluminum foil, they were sealed with Z.O.E. cement. In experimental group 2, only aucubin powder was applied on the remaining pulps and then they were treated the same as experimental group 1. In the all groups, the pulps were histopathologically observed by light microscope at the time intervals of 1, 2 and 4 weeks after experiment. The results were as follows : 1. In control and experimental groups, mild vascular congestion and bleeding were found in most of the specimens. Less inflammatory infiltration was observed in experimental groups than in control group. 2. Dentin bridge formation was found after 1 week at both control and experimental group 1. Dentin birdge had discontinuous osteodentin like appearance or contained some dentin chips. In experimental group 2, dentin bridge was not seen. 3. The coagulation necrosis layer on the remaining pulp tissues was seen in all groups. In experimental group 2, the thickest layer was observed. And in control group, coagulation necrosis layer was similar as in experimental group 1.

• Restorative Dentistry and Endodontics
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• 제14권1호
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• pp.109-118
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• 1989

The purpose of this study was to investigate the bio-stimulating effect of low power density laser radiation on the mechanically exposed pulp. Class V cavities on dog's teeth were prepared and the pulps were mechanically exposed with a round bur. In control group, the exposed pulps were capped with $Ca(OH)_2$ powder and the cavities were sealed with Z.O.E.. In experimental group A, the pulps were irradiated with GaA1As laser for 5 minutes and then they were treated the same as control group. In experimental group B, the exposed pulps were covered by aluminum foil and sealed with Z.O.E. after they were irradiated with the laser as the experimental group A. In the all groups, the pulps were histopathologically observed at the time intervals of 1, 2 and 3 week after experiment and the results were statistically evaluated. The results were as follows: 1) In control and experimental groups, mild vascular congestion and bleeding was found in most of the specimens and for the new formation of dentin bridge, experimental group A had the most cases. The dentin bridge had discontinuous osteodentin like appearance without any dentinal tubules. Inflammatory cell infiltration consisted of acute and chronic inflammatory cell, and the formation of microabscess was also observed. 2) The degree of inflammatory cell infiltration was not significantly different among control group and experimental groups at 1 week, 2 week and 3 week. 3) The formation of new dentin bridge was not significantly different between control group and experimental group A at 1 week, but at 2 week and 3 week, experimental group A showed significantly more cases of new dentin formation than control groups. (P < 0.05). 4) Between control group and experimental group B, there was no significant difference in formation of the new dentin bridge at 1, 2 and 3 week. (P> 0.05). 5) There was no significant difference in formation of the new dentin bridge at 1 and 2 week between experimental group A and experimental group B, but at 3 week, the former significantly had more cases of new dentin bridge formation than the latter.(P < 0.05).

• 대한소아치과학회지
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• 제32권4호
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• pp.662-669
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• 2005

NFI-C는 정상적으로 상아모세포, 골모세포에 존재하며, NFI-C가 결손된 경우 치근상아질을 형성하는 상아모세포의 분화에 이상이 있다고 알려져 있다. 본 연구는 NFI-C (-/-) 생쥐에서 치관 및 치근의 상아질을 형성하는 상아모세포의 표현형을 상아모세포에 특이적으로 발현하는 DSPP와 골모세포에 특이적으로 반응하는 BSP 유전자를 이용한 in-situ hybridization을 통하여 연구하여 다음과 같은 결과를 얻었다. 1. NFI-C (-/-) 생쥐의 구치에서 치관 상아질은 정상적으로 형성되었으나 치근 상아질은 형성되지 않았다. 2. NFI-C (-/-) 생쥐의 하악 절치의 순측 상아질은 비교적 많이 형성되었으나, 설측의 상아질은 형성되지 않았다. 3. NFI-C (-/-) 생쥐의 하악 절치의 상아모세포는 형태가 변화되었으며, 형성된 상아질 내에 세포가 함입되는 양상을 보였다. 4. NFI-C (-/-) 생쥐의 구치에서 치관부위의 상아모세포에서는 DSPP가 강하게 발현되었으나, 치근부위의 상아모세포에서는 발현되지 않았다. 또한 NFI-C (-/-) 생쥐에서 절치의 순측 상아모세포에서는 DSPP가 약하게 발현되었다. 5. 정상 생쥐에서 절치의 상아모세포에서는 BSP가 발현되지 않았으나 NFI-C (-/-) 생쥐에서는 BSP가 강하게 발현되었다. 이상의 결과를 종합하여 볼 때 NFI-C가 결손된 경우 상아모세포가 골모세포로 분화되는 표현형의 변화를 보이는 것으로 사료된다.

• 대한소아치과학회지
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• 제33권2호
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• pp.181-191
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• 2006

NFI-C K/O 생쥐에서는 상아모세포의 분화과정에 이상이 초래되어 상아질 형성에 이상이 생기고 치근 형성이 불완전하게 이루어지는 것으로 알려져 있으나 이에 대한 명확한 기전은 잘 알려져 있지 않다. 상아모세포가 분화하여 정상적으로 상아질을 형성하기 위하여 핵과 세포질이 극성을 띠고 잘 조직화되어야 하며, 이 과정에는 다양한 세포사이 결합장치들이 중요한 역할을 하는 것으로 알려져 있다. 본 연구에서는 NFI-C K/O 생쥐에서 상아질 형성에 이상이 생기는 것이 상아모세포의 형태학적 변화와 세포사이 결합장치들이 기능을 하지 못한 결과에서 기인한 것인지 알아보기 위하여, NFI-C K/O 생쥐에서 발생한 비정상적인 상아모세포들을 광학 및 투과 전자현미경을 이용하여 형태학적으로 관찰하고, Zo-1과 occludin의 발현을 면역조직화학적으로 관찰하여 세포사이 결합장치들의 분포를 확인하여 다음과 같은 결과를 얻었다. 1. 광학현미경 소견에서 NFI-C K/O생쥐의 전치부 상아모세포는 세포 극성이 상실되고, 여러 층으로 배열되어 있었으며 상아질에 많은 세포들이 함입된 것과 같은 비정상적인 상아질의 소견을 나타냈다. 반면에 NFI-C K/O생쥐의 구치부 상아모세포는 치관부에서는 잘 조직화된 소견을 보였으나 치근 형성 부위 에서는 세포 배열이 불규칙해지고 세포 극성이 상실되었다. 2. 투과 전자현미경 소견에서 NFI-C K/O생쥐 전치부의 비정상적인 상아모세포는 둥근 형태로 세포 사이 간격이 넓으며 폐쇄연접과 같은 세포사이 결합장치들이 전혀 관찰되지 않았다. 3. ZO-1의 면역조직화학적 염색에서 NFI-C K/O생쥐의 전치부 법랑모세포의 근위부와 원위부에서 ZO-1이 강하게 발현되었으나 비정상적인 상아모세포에서는 ZO-1의 발현을 관찰할 수 없었다. 4. Occludin의 면역조직화학적 염색에서 정상 생쥐의 전치부 상아모세포에서는 occludin의 발현이 관찰되었으나 NFI-C K/O생쥐의 비정상적인 상아모세포에서는 occludin의 발현이 관찰되지 않았다. 이상의 결과를 종합하여 볼 때 NFI-C의 결손은 상아모세포의 분화 이상을 초래하고 비정상적으로 상아질을 형성하는 과정에 세포사이 결합장치의 상실과 같은 형태학적인 변화의 중요한 요소로 작용하는 것으로 생각된다.