• Proceedings of the Korean Society of Crop Science Conference
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• 2005.04a
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• pp.34-35
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• 2005

• Proceedings of the Korean Society of Crop Science Conference
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• 2005.04a
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• pp.214-215
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• 2005

• Genomics & Informatics
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• v.5 no.4
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• pp.194-195
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• 2007

We have constructed the Rice Genome Information Service System (RGISS), which is an information service system of the Oryza sativa L. ssp. japonica (rice) genome, using the released version of rice Build 3.0 pseudomolecules based on the Ensembl architecture. The nonredundant library, composed of 3,360 clones of BACs, PACs, and fosmids, was used to construct supercontigs. RGISS contains 50,717 annotated genes from GenBank, 56,161 predicted genes from FgeneSH, and information on 9,587 markers, which includes STS, SSR, and EST-based RFLP. The 20,180 ESTs sequenced by the Korea National Institute of Agricultural Biotechnology (NIAB) were aligned and mapped into 168,792 exons. By gene ontology analysis, the classified protein numbers in the rice genome were 6158, 4531, and 12,364 proteins, which were mapped to molecular function, cellular component, and biological process, respectively.

• Proceedings of the Korean Society of Crop Science Conference
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• 2005.10b
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• pp.130-131
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• 2005

• Proceedings of the Korean Society of Crop Science Conference
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• 2005.10b
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• pp.318-319
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• 2005

• Molecules and Cells
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• v.25 no.2
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• pp.312-316
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• 2008

Flavanone $3{\beta}$-hydroxylases (F3H) are key enzymes in the synthesis of flavonol and anthocyanin. In this study, three F3H cDNAs from Oryza sativa (OsF3H-1 ~3) were cloned by RT-PCR and expressed in E. coli as gluthatione S-transferase (GST) fusion proteins. The purified recombinant OsF3Hs used flavanone, naringenin and eriodictyol as substrates. The reaction products with naringen and eriodictyol were determined by nuclear magnetic resonance spectroscopy to be dihydrokaempferol and taxifolin, respectively. OsF3H-1 had the highest enzymatic activity whereas the overall expression of OsF3H-2 was highest in all tissues except seeds. Flavanone $3{\beta}$-hydroxylase could be a useful target for flavonoid metabolic engineering in rice.

• Korean journal of applied entomology
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• v.34 no.2
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• pp.132-139
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• 1995

The present work was dealt with the twelve species of orbatid mites from leaves, stems and stubbles of rice( Oryza sativa L). Among them six species, that is, Protoribates agricola, Scheloribates decarinatus, Scheloribates oryzae, Sacculozets filosus, Punctoribates manzanoensis, and Paralamellobates ceylanica are new ones in Korea. The other species are Epilohmannia palida pacifica, Podorbates cuspidatus, Tricogaulumna nipponica, Peloribate sp., Scheloribates sp., Galumna sp.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.4
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• pp.362-368
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• 2006

A pot experiment was conducted to investigate the effects of soil burial depth on seedling emergences of rice (Oryza sativa) and Echinochloa spp. and to model such effects for mathematical prediction of seedling emergences. When the Gompertz curve was fitted at each soil depth, the parameter C decreased in a logistic form with increasing soil depth, while the parameter M increased in an exponential form and the parameter B appeared to be constant. The Gompertz curve was combined by incorporating the logistic model for the parameter C, the exponential model for the parameter M, and the constant for the parameter B. This combined model well described seedling emergence of rice and Echinochloa species as influenced by soil burial depth and predicted seedling emergence at a given time after sowing and a soil burial depth. Thus, the combined model can be used to simulate seedling emergence of crop sown in different soil depths and weeds present in various soil depths.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.4
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• pp.348-355
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• 2006

Phenotypes of panicle, hull and seed of mutant rice (Oryza sativa L.) were characterized. Panicle mutants were classified in 4 groups with their internode length of main rachis, primary rachis, secondary rachis and pedicel. Hull and seed mutants were grouped into 12 based on their mutant characters in shape, size and color of seeds. These natural and spontaneous mutant collections showed distinct phenotypes to wild type rice. This might be useful for the identification of the functions of genetic factors in the Mendelian inheritance.

• Journal of Crop Science and Biotechnology
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• v.11 no.4
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• pp.233-236
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• 2008

A positive selectable marker system was adapted for transformation of mature embryo-derived calli of Indica rice (Oryza sativa L.) utilizing the PMI gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate. The transformed cells grew on medium supplemented with 3% mannose as carbon source and calli were selected on media containing various concentrations of mannose. Molecular analyses showed that the transformed plants contained the PMI gene. The results indicate that the mannose selection system can be used for Agrobacterium-mediated transformation of mature embryo in rice to substitute the use of conventional selectable markers in genetic transformation.