• International Journal of Stem Cells
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• v.16 no.3
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• pp.342-355
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• 2023

Background and Objectives: Osteoblasts are derived from bone marrow mesenchymal stem cells (BMMSCs) and play important role in bone remodeling. While our previous studies have investigated the cell subtypes and heterogeneity in osteoblasts and BMMSCs separately, cell-to-cell communications between osteoblasts and BMMSCs in vivo in humans have not been characterized. The aim of this study was to investigate the cellular communication between human primary osteoblasts and bone marrow mesenchymal stem cells. Methods and Results: To investigate the cell-to-cell communications between osteoblasts and BMMSCs and identify new cell subtypes, we performed a systematic integration analysis with our single-cell RNA sequencing (scRNA-seq) transcriptomes data from BMMSCs and osteoblasts. We successfully identified a novel preosteoblasts subtype which highly expressed ATF3, CCL2, CXCL2 and IRF1. Biological functional annotations of the transcriptomes suggested that the novel preosteoblasts subtype may inhibit osteoblasts differentiation, maintain cells to a less differentiated status and recruit osteoclasts. Ligand-receptor interaction analysis showed strong interaction between mature osteoblasts and BMMSCs. Meanwhile, we found FZD1 was highly expressed in BMMSCs of osteogenic differentiation direction. WIF1 and SFRP4, which were highly expressed in mature osteoblasts were reported to inhibit osteogenic differentiation. We speculated that WIF1 and sFRP4 expressed in mature osteoblasts inhibited the binding of FZD1 to Wnt ligand in BMMSCs, thereby further inhibiting osteogenic differentiation of BMMSCs. Conclusions: Our study provided a more systematic and comprehensive understanding of the heterogeneity of osteogenic cells. At the single cell level, this study provided insights into the cell-to-cell communications between BMMSCs and osteoblasts and mature osteoblasts may mediate negative feedback regulation of osteogenesis process.

• Korean Institute of Interior Design Journal
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• v.22 no.6
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• pp.20-28
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• 2013

This study attempts to grasp outpatients' demands for interior space of outpatients departments through evaluating them. Six departments in H general hospital such as surgery, orthopedics, neurosurgery, plastic surgery, obstetrics, and urology, were evaluated. Evaluating items were consisted of ones related to spatial layout, furniture, interior climate, color and finishes, and dressing room of each waiting area, doctor's room and treatment room in six departments. The subjects were 210 outpatients who were using one of six departments. The results were ; 1) The outpatients evaluated very high interior space of waiting areas in six departments. 2) They evaluated doctor's rooms to be relatively high. However, visual privacy in there was not enough met. 3) The treatment room was also evaluated relatively high. However, furniture and equipment arrangement, consideration for patients and a dressing room were evaluated relatively low. 4) The outpatients' evaluation was significantly different by their gender and age. The male evaluated them higher than the female. Also, the older patients evaluated them higher than the younger. 5) The outpatients responded that ventilation, comfort interior, and space arrangement were very important elements in healthcare facility design.

• Journal of Korean Academy of Nursing Administration
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• v.8 no.4
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• pp.655-667
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• 2002

Purpose : This study analysis of nursing interventions and core nursing interventions performed orthopedic surgery care unit. Method : The data were collected from 55 nurses of 4 general hospital from July. 10, 2002 to Aug. 30, 2002. The instrument for this study was the Korean translation of 486 nursing intervention classifications developed by McColskey & Bulechek in 2000. In the 486 nursing interventions 350 nursing interventions were selected 8 among the 10 more than 5 years working group in orthopedic surgery care unit. Self frequency checking performed by 350 nursing interventions were used secondary questionnaire In the orthopedic surgery care unit nurses. Results : 1. The most frequently used nursing interventions domains were "Physiological : basic" and then "Physiological: complex", "Health system" "Behavior", "Family", "Safety". 2. Orthopedic surgery care unit core nursing interventions were performed several times a day by 50% or more of orthopedic surgery care unit and this result were 4 of domain, 11 of class, 26 core nursing interventions. This core nursing interventions were 5 of "Physiological : basic", 17 of "Physiological: complex", 1 of "Safety", 3 of "Health system" and have no "Behavior", "Family". Conclusion : This study analyzed nursing intervention of orthopedics and reestablished nursing intervention concept and I hope this study will be helpful for the nurse to be a professional by applying this to actual clinic, for development in qualified nursing and for establishment nursing information system.

• Journal of mucopolysaccharidosis and rare diseases
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• v.3 no.1
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• pp.33-33
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• 2017

• Journal of muscle and joint health
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• v.24 no.2
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• pp.67-76
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• 2017

Purpose: The purpose of this study was to evaluate preoperative nutritional status in elderly patients with orthopedic surgery and identify related factors for malnutrition risk. Methods: This study enrolled 337 patient's medical record who underwent orthopedic surgery in hospital between January and December 2015. Data was collected retrospectively. Nutritional status was evaluated by using the Nutritional Risk Screening 2002. Multivariable logistic regression analysis was used to identify independent related factors for malnutrition risk. Results: Malnutrition risk developed in 58 patients (17.2%). Logistic regression analysis identified low physical activity, visual impairment, depression, sleep disorder, low serum calcium level, and low serum albumin level as related factors. Conclusion: Orthopedic surgery in elderly patients was associated with high risk of preoperative malnutrition. The results of this study suggest that evaluating the nutritional status and related factors should be done with preoperative status of elderly patients. At the same time, interventions for nutritional care should be adjusted to meet the nutritional needs of individuals and decrease the risk of malnutrition.

• The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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• v.26 no.1
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• pp.73-78
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• 2020

Background: The leg length discrepancy (LLD) have been implicated with degenerative diseases since the early 1970s, which also causes musculoskeletal asymmetry problems. The LLD appears due to factors such as balance ability, response time, and muscle strength between the shorter and longer leg. In this study, the effect of manual therapy to LLD has been studied. Methods: Manual therapy has been applied on shorter leg of 30 patients with LLD. The manual therapy was conducted for 2~3 minutes 3 times a week for 4 weeks. Results: The leg length of shorter leg were measured at initial baseline and after 4 weeks. the average leg length of shorter leg increased from 94.10 cm to 94.33 cm. Conclusion: This paper reports that the manual therapy has positive effects on LLD. It was studied by conducting manual therapy on muscles and joints related to shorter leg. In the future, the muscles and joints of the longer leg should be considered and various age group should be studied to develop the understanding of the effect of manual therapy on LLD.

• Physical Therapy Korea
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• v.4 no.2
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• pp.66-76
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• 1997

The purpose of this study was to compare the integrated electromyographic activity ratios of vastus lateralis(VL); rectus femoris lateral portion (RFL); rectus femoris medial portion(RFM); and vastus medialis(VM) muscles of 30 healthy subjects under three proprioceptive neuromuscular facilitation(PNF) techniques. Each subject was randomly assigned to one of 3 PNF techniques groups : slow reversal(SR), slow reversal hold(SRH) and rhythmic stabilization (RS). Each person was positioned in supine with the right hip flexed to $45^{\circ}$ and the knee fully extended and received a total of 6 sessions. Each technique was applied to the right lower extremity in two diagonal patterns while electrical activity was monitored from the ipsilateral muscles VL, RFL, RFM, and VM, respectively. Comparison of normalized mean EMG magnitudes from VL, VM showed that RS demonstrated significantly greater activity than that of SR or SRH and that RFL and RFM did not demonstrate any greater relative EMG activity with the three PNF techniques than did VL or VM.

• Biomolecules & Therapeutics
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• v.24 no.3
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• pp.291-297
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• 2016

Cytisine (CYT), a partial agonist of ${\alpha}4{\beta}2-nicotinic$ receptors, has been used for antidepressant efficacy in several tests. Nicotinic receptors have been shown to be closely associated with depression. However, little is known about the effects of CYT on the depression. In the present study, a mouse model of depression, the unpredictable chronic mild stress (UCMS), was used to evaluate the activities of CYT. UCMS caused significant depression-like behaviors, as shown by the decrease of total distances in open field test, and the prolonged duration of immobility in tail suspension test and forced swimming test. Treatment with CYT for two weeks notably relieved the depression-like behaviors in the UCMS mice. Next, proteins related to depressive disorder in the brain region of hippocampus and amygdala were analyzed to elucidate the underlying mechanisms of CYT. CYT significantly reversed the decreases of 5-HT1A, BDNF, and mTOR levels in the hippocampus and amygdala. These results imply that CYT may act as a potential anti-depressant in the animals under chronic stress.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.06a
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• pp.915-918
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• 2005

Advances in Information Technology and in Biomedicine have created new uses for CAD technology with many novel and important biomedical applications. Such applications can be found, for example, in the design and modeling of orthopedics, medical implants, and tissue modeling in which CAD can be used to describe the morphology, heterogeneity, and organizational structure of tissue and anatomy. CAD has also played an important role in computer-aided tissue engineering for biomimetic design, analysis, simulation and freeform fabrication of tissue scaffolds and substitutes. And all the applications require precision geometry of the organs or bones of each patient. But the geometry information currently used is polygon model with none solid geometry and is so rough that it cannot be utilized for accurate analysis, simulation and fabrication. Therefore a case study is performed to deduce a transformation method to build free form surface from a rough polygon data or medical images currently used in the application. This paper describes the transformation procedure in detail and the considerations for accurate organ modeling are discussed.

• Journal of the Korean Society of School Health
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• v.21 no.2
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• pp.107-117
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• 2008

Purpose: To investigate the utilization of medical care benefits of school personnel on duty in Seoul. Method: The subjects were 551 workers under Seoul Metropolitan Office of Education(male 301, female 250) who got medical care benefits from March 2000 to February 2005. To analyze the data, frequency, chi-square test, and t-test by SAS package 9.12 were used. Result: The results show that elementary school personnel got the highest ranking, 314(57.0%). Among subjects, 57.2% personnel got accident when they were working on their own duty, 21% during school events and 13.5% were during commutes. They got medical care from orthopedics 75.9%, neurosurgery 7.6%, dental 4%, and 8 male and 2 female died during these days. The most frequent diseases of males and female were fracture and joints related disease. The reason of most health problem was due to injury(92.3%). Conclusion: The results of this study suggest that the continuous health care services like regular health check for school personnel, or social safety network to prevent injury are needed.