• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2018년도 추계학술대회
• /
• pp.113-113
• /
• 2018

Endophthalmitis is a disease that causes ocular inflammation and has a catastrophic effect on eyesight. Recent studies show that Enterococcus faecalis is rapidly increasing causative bacterium of endophthalmitis. It is predicted that the increased endophthalmitis by E. faecalis is presumable due to the high resistance of E. faecalis to moxifloxacin (MFX), which is a common antibiotic used for eye drop. Because of the need for therapeutic agents to overcome this problem, this study sought to explore the feasibility of developing a combination therapy using Orostachys japonicus. The ethylacetate fraction of O. japonicus (OJA) used in this study. Antimicrobial activity was tested 13 E. faecalis strains including one E. faecalis standard strain, eight clinically isolated E. faecalis strains and four quinolone resistant E. faecalis strains using CLSI antibiotic susceptibility test method. Minimal Inhibitory Concentration (MIC) of OJA was confirmed to be $500{\mu}g/ml$ for all 13 strains. Then we tested for the synergistic effect of OJA to MFX using checkboard test method. The MIC of MFX was $0.25{\mu}g/ml$ for the standard strain and 8 for the clinical isolates, and $16{\sim}64{\mu}g/ml$ for the quinolone - resistant strains. When OJA was mixed with MFX, no synergistic effect was observed in all strains, but the antibacterial activity of OJA remained unchanged. Most ocular other strains can be removed by MFX except the MFX resistant E. faecalis, which can be removed by OJA in combination therapy. Therefore, OJA can be a potential candidate for the combined treatment endophthalmitis.

• Journal of Acupuncture Research
• /
• 제18권1호
• /
• pp.88-99
• /
• 2001

Objectives : This study was performed to determine if Orostachys japonicus A. Berger aquacupuncture (OjB) provides the protective effect against the loss of celi viability and DNA damage induced by oxidant in renal proximal tubular cells. Methods : The cell viability was evaluated by a MTT reduction assay and DNA damage was estimated by measuring double stranded DNA breaks in opossum kidney (OK) cells, an established proximal tubular cell line. Lipid peroxidation was determined by measuring malondialdehyde (MDA), a product of lipid peroxidation. Results : $H_2O_2$ increased the loss of cell viability in a time-dependent manner, which were prevented by 0.1% OjB. The protective effect of OjB was dose-dependent over concentration range of 0.05-0.5%. $H_2O_2$ caused ATP depletion and DNA damage, which were prevented by OjB and the hydrogen peroxide scavenger catalase. The loss of cell viability by $H_2O_2$ was not affected by the antioxidant DPPD, but lipid peroxidation by the oxidant was completely inhibited by DPPD. Conclusions : These data suggest that $H_2O_2$-induced death results from a lipid peroxidation-independent mechanism and the protective effect of OjB is not associated with its antioxidant activity.

• BMB Reports
• /
• 제43권11호
• /
• pp.750-755
• /
• 2010

Crude Orostachys japonicus polysaccharide extract (OJP) was prepared by hot steam extraction. Polysaccharides (OJPI) were separated from OJP by gel filtration chromatography and phenol-sulfuric acid assay. The average molecular weight of the OJPI was 30-50 kDa. The anti-proliferative effect of OJPI on HT-29 human colon cancer cells was investigated via morphology study, cell viability assay, apoptosis assay, cell cycle analysis, and cDNA microarray. OJPI inhibited proliferation and growth of HT29 cells and also stimulated apoptosis in a dose- and time-dependent manner. In cell cycle analysis, treatment with OJPI resulted in a marked increase of cells in the G0 (sub G1) and G2/M phases. To screen for genes involved in the induction of cell cycle arrest and apoptosis, the gene expression profiles of HT-29 cells treated with OJPI were examined by cDNA microarray, revealing that a number of genes were up- or down-regulated by OJPI. Whereas several genes involved in anti-apoptosis, cell proliferation and growth, and cell cycle regulation were down-regulated, expression levels of several genes involved in apoptosis, tumor suppression, and other signal transduction events were up-regulated. These results suggest that OJPI inhibits the growth of HT-29 human colon cancer cells by various apoptosis-aiding activities as well as apoptosis itself. Therefore, OJPI deserve further development as an effective agent exhibiting anticancer activity.

• 한국약용작물학회지
• /
• 제14권4호
• /
• pp.239-243
• /
• 2006

개화 이후 고사하는 일임성 식물인 바위솔은 가을철에 집중되는 자연산의 출하시기와 중복되지 않도록 비가림 하우스에서 인공재배되고 있다. 본 연구는 하우스 시설로 인한 차광 (무차광, 35, 55, 75, 95% 차광)이 바위솔의 생장 및 개화에 미치는 영향을 파악하고자 실시되었던 바 그 결과를 요약하면 다음과 같다. 1. 초장, 화서장, 엽과 포엽수 및 경직경은 광도가 가장 약한 95% 차광시 가장 짧고, 적거나, 가늘었던 반면, 여타 차광 처리간에는 차이가 적다 할지라도 차광이 심할수록 줄어드는 경향을 보였다. 2. 엽과 포엽중, 경중, 근중, 지상부중, 전체건물중은 무차광에서 가장 많았으며 차광이 심할수록 감소되는 경향을 보였다. 3. 형성된 소화수는 건물중과 같이 무차광에서 가장 많고 차광이 심할수록 감소되었으나, 95% 차광시 아주 적었다. 4. 차광처리로 소화가 개화된 개체는 관찰되지 않아 광도가 제한되는 시설하우스에서 여타 처리를 통하여 바위솔의 인공재배는 가능할 것으로 판단된다.

• Natural Product Sciences
• /
• 제17권2호
• /
• pp.117-122
• /
• 2011

We investigated the effect of Orostachys japonicus extracts on serum lipids, leptin and insulin level in hyperlipidemic rats. Also, diacylglycerol acyltransferase (DGAT) activity and thiobarbituric acid reactive substance (TBARS) were assessed. Inhibitory effect of DGAT related to triglyceride synthesis emerged approximately 96% in EtOAc fraction and showed 90% and 67%, respectively, in CHCl3 and BuOH fractions. Furthermore, the EtOAc and BuOH fractions inhibited 81% and 77%, respectively, in glycerol-3-phosphate acyl transferase (GPAT). Hyperlipidemia and obesity marker, contents of leptin and insulin on serum of hyperlipidemic rats, decreased 50% and 25%, respectively, compared with control group in treated EtOAc fraction. The oxidative stress marker, a concentration of TBARS, showed decrease of approximately 30% in treated EtOAc fraction. Moreover, high density lipoprotein (HDL)-cholesterol contents on serum of rats fed a hyperlipidemic diet were increased 10% and low density lipoprotein (LDL)-cholesterol decreased 50% as well as triglyceride amount of feces multiplied approximately two times more than control group in treated EtOAc fraction. The data suggest that the fractions of O. japonicus may be a potent biomaterial for treatment of hyperlipidemia or obesity.

• 한국식품영양학회지
• /
• 제21권2호
• /
• pp.148-156
• /
• 2008

The purpose of this study was to analyze the changes in physicochemical characteristics and NDMA formation of Orostachys japonicus A. Berger according to harvest times. The results showed moisture, carbohydrate, crude fiber and crude protein contents of <$48.5{\pm}1.4%$, $38.2{\pm}1.2%$, $l5.8{\pm}0.4%$ and $13.2{\pm}0.6%$ respectively. The total mineral content was 4,817.1 mg/100 g, where Ca was highest($2,577.0{\pm}3.2$ mg/100 g), followed by K, Mg, Na, P and Fe. The composition amino acid of O. japonicus A. Berger showed high levels of glutamic acid and aspartic acid. And among the five free sugars detected, galactose and glucose were most abundant at $32.2{\pm}0.02$ mg/100 g and $25.3{\pm}0.1O$ mg/100 g, respectively. Upon comparing O. japonicus A. Berger extracts that came from plants harvested during $August{\sim}October$, total phenolic compound($2,703.l{\pm}5.2l{\sim}2,428.0{\pm}3.52$ mg/100 g) and flavonoid($634.2{\pm}2.33{\sim}1,324.6{\pm}1.87$ mg/100 g) contents were higher in the methanol extract than in the water extract. Also, within a reaction system, nitrite scavenging ability and NDMA inhibition were most effective at pH 2.5, and increased in proportion to the extract concentration. Finally, the methanol extract of O. japonicus A Berger harvested during $August{\sim}October$ had the highest nitrite scavenging and NDMA inhibition effects.

• 식물분류학회지
• /
• 제33권4호
• /
• pp.359-371
• /
• 2003

한국산 돌나물과 바위솔속 내의 종 내 변이를 조사하고 종간의 유연관계를 밝히고자 4종 14집단으로부터 28개의 형태형질을 측정하여 수리분석을 실시하였다. 유집분석 결과, 크게 3개의 유집을 형성하였고, 둥근바위솔, 연화바위솔, 좀바위솔 집단은 가까운 유집을 형성하여 유연관계가 깊은 것으로 생각된다. 객산리(I)와 가덕도에서 채집된 바위솔은 나머지 바위솔 집단과는 독립된 유집을 형성하여 바위솔 내 형태적 분화가 심하며 이는 기존의 세포학적인 변이와도 연관이 있을 것으로 추측된다. 정동진에서 채집된 둥근바위솔 집단은 기존의 동일종 집단과 유집되지 않고, 바위솔 집단에 가깝게 유집되어 형태학적으로 이질적 집단임을 알 수 있다. 형태형질을 이용한 주성분분석 결과, 바위솔 집단과 나머지 집단들로 크게 둘로 나누어지며, 둥근바위솔과 좀바위솔, 연화바위솔 집단은 서로 가깝게 배열되었다.

• 대한한의학회지
• /
• 제40권2호
• /
• pp.35-50
• /
• 2019

Objectives: Orostachys japonicas (O. japonicus) has been known for its anti-tumor effect. In the present study, it was investigated whether O. japonicus EtOH extracts could induce apoptosis and autophagy which are part of the main mechanism related to anti-tumor effect in THP-1 cells. Methods: Cells were treated with various concentrations of O. japonicus EtOH extracts ($0-300{\mu}g/ml$) for 24, 48, and 72h. Cell viability was evaluated by MTS/PMS assay and apoptosis rate was examined by flow cytometry and ELISA assay. The mRNA expression of apoptosis-related genes (Bcl-2, Mcl-1, Survivin, Bax) and autophagy-related gene (mTOR) was evaluated using real-time PCR. The protein expression of Caspase-3, Akt, LC3 II, Beclin-1, Atg5, $NF-{\kappa}B$, p38, ERK was evaluated using western blot analysis. Results: O. japonicus EtOH extracts inhibited cell proliferation and apoptosis rate was increased in both flow cytometry and ELISA assay. Bcl-2, Mcl-1, Survivin (anti-apoptosis factors) mRNA expressions were decreased and Bax (pro-apoptosis factor) mRNA level was increased. mTOR mRNA expressions was decreased and LC3 II protein expressions was increased. Activation of $NF-{\kappa}B$ was decreased and phosphorylation of p38 was increased. Conclusion: O. japonicus is regarded to inhibit cell proliferation, to induce apoptosis and to regulate autophagy-related genes in THP-1 cells via $NF-{\kappa}B$ and p38 MAPK signaling pathway. This suggests O. japonicus could be an effective herb in treating acute myeloid leukemia.

• 대한본초학회지
• /
• 제29권4호
• /
• pp.29-34
• /
• 2014

Objectives : Ulcerative colitis (UC) is an inflammatory bowel disease, which is one of chronic gastrointestinal disorders. Orostachys japonicus (OJ) has been used as a traditional medicine for various diseases including gastric cancer, gastric ulcers and intoxication. However, the regulatory effect of OJ on intestinal inflammation has not been fully understood, yet. The aim of this study was to investigate the effect of OJ on dextran sulfate sodium (DSS)-induced colitis in mice. Methods : To ascertain the pharmacological effects of OJ, the colitis mice were induced by drinking water containing 5% DSS for 7 days. Mice were randomized into groups receiving OJ (100 mg/kg), sulfasalazine (150 mg/kg) as a positive control, or water as a negative control. We evaluated the effects of OJ on DSS-induced the clinical signs, measuring weight loss and colon length. In addition, the inhibitory effect of OJ on the tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) level was determined by enzyme-linked immunosorbent assay in colitis tissue. Results : The results indicated that mice treated with DSS showed remarkable clinical signs, including weight loss, and reduced colon length. However, treatment with OJ significantly improved the weight loss and DAI as clinical symptoms. Moreover, OJ reduced the TNF-${\alpha}$ levels in DSS-treated colon tissues. Conclusions : Collectively, the findings of this study provide us with novel insights into the pharmacological actions of OJ as a potential medicine for use in the treatment of ulcerative colitis.

• 한국식품과학회지
• /
• 제49권5호
• /
• pp.524-531
• /
• 2017

본 연구에서는 와송(Orostachys japonicus A. Berger)의 산화방지 효과와 산화스트레스로부터의 신경세포 손상에 대한 보호효과 및 주요 생리활성물질을 분석하였다. 와송 아세트산에틸 분획물(ethylacetate fraction from Orostachys japonicus A. Berger extract: EFOJ)은 다른 분획물보다 높은 총 페놀화합물 함량을 나타냈으며, 와송 아세트산에틸 분획물(EFOJ)로 ABTS, DPPH 라디칼 소거 활성과 FRAP 검정 및 지방질과산화물 억제력 분석을 실시한 결과, 우수한 라디칼 소거 활성, 총산화방지력과 과산화 지방질 생성물의 억제력을 확인하였다. 또한 와송 아세트산에틸 분획물(EFOJ)은 과산화수소로 인한 신경세포 사멸에 대한 세포 생존율을 향상시켰으며, 신경 세포막 손상을 보호하는 능력이 우수한 것을 확인하였다. 이러한 생리 활성을 가진 와송 아세트산에틸 분획물(EFOJ)의 주요물질을 분석하기 위하여 $LC-MS^e$를 실시하였으며, 주요 생리활성 물질은 퀘세틴 배당체와 캠페롤 배당체로 추정되었다. 본 연구 결과들을 바탕으로, 와송 추출물은 우수한 산화방지력을 가지고 있을 뿐만 아니라 산화적 스트레스로부터의 신경세포 보호효과를 통해 퇴행성 신경질환과 같은 질병을 예방 할 수 있는 건강기능성식품의 천연산화방지제 소재로써 고부가 가치가 있다고 판단된다.