Journal of Acupuncture Research

  • 제18권1호
  • /
  • Pages.88-99
  • /
  • 2001
  • /
  • 2586-288X(pISSN)
  • /
  • 2586-2898(eISSN)
대한침구의학회 (Korean Acupuncture & Moxibustion Medicine Society)
권호 표지

와송약침액(瓦松藥鍼液)이 신장세포(腎臟細胞)에서 H2O2에 의한 세포사망(細胞死亡) 및 DNA 손상(損傷)에 미치는 영향(影響)

The Effects of Orostachys Japonicus A. Berger Aquacupuncture on Cell Death and DNA Damage Induced by H2O2 in Renal Tubular Cell

  • 박상원 (동의대학교 한의과대학 경혈학교실) ;
  • 송춘호 (동의대학교 한의과대학 경혈학교실)
  • Park, Sang-Won (Department of AM-Meridian & Pointology, College of Oriental Medicine, Dong-Eui University) ;
  • Song, Choon-Ho (Department of AM-Meridian & Pointology, College of Oriental Medicine, Dong-Eui University)
  • 투고 : 2001.01.07
  • 심사 : 2001.01.17
  • 발행 : 2001.02.20
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초록

Objectives : This study was performed to determine if Orostachys japonicus A. Berger aquacupuncture (OjB) provides the protective effect against the loss of celi viability and DNA damage induced by oxidant in renal proximal tubular cells. Methods : The cell viability was evaluated by a MTT reduction assay and DNA damage was estimated by measuring double stranded DNA breaks in opossum kidney (OK) cells, an established proximal tubular cell line. Lipid peroxidation was determined by measuring malondialdehyde (MDA), a product of lipid peroxidation. Results : $H_2O_2$ increased the loss of cell viability in a time-dependent manner, which were prevented by 0.1% OjB. The protective effect of OjB was dose-dependent over concentration range of 0.05-0.5%. $H_2O_2$ caused ATP depletion and DNA damage, which were prevented by OjB and the hydrogen peroxide scavenger catalase. The loss of cell viability by $H_2O_2$ was not affected by the antioxidant DPPD, but lipid peroxidation by the oxidant was completely inhibited by DPPD. Conclusions : These data suggest that $H_2O_2$-induced death results from a lipid peroxidation-independent mechanism and the protective effect of OjB is not associated with its antioxidant activity.

키워드

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