• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.138-138
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• 2017

Agriculture is the most primitive civilized Activities of mankind but also the propellant of civilization development. Because it is the most basic material goods source of mankind. Among these materials rice is one of the most important part of these, we call them the substance of survival. From the beginning of the agricultural activities to the present we have experienced three industrial revolutions and are experiencing the Fourth Industrial Revolution. With the development of science and technology makes the efficiency of agricultural production is higher and higher, but compared with the original we are facing the same problem: natural disasters; pests and diseases; now also face the depletion of resources, environmental degradation and other issues. Therefore, improve and cultivate new crop varieties to make it better resistance and more production for better develop modern agriculture. It's very helpful for human social development. And also it is the responsibility and task of modern molecular breeding. In this study, I used bacterial leaf blight to find a better resistance gene to improve the resistance of rice. Frist Cultivate k3 of bacterial leaf blight, than inoculation by leaf clipping method (Kauffman,1973) in CNDH and SNDH population at 40days after rice transplanting. Check the lesion length by inoculation plants at 14days after inoculation, and record data for QTL analysis program. Than I get 4 intervals in 3 different chromosomal regions. I found these defense genes in the 4 intervals. So I used NCBI Justbio, Rapdb, etc. to finding these genes in physical map, than design primer for map base cloning. At last these defense genes will be employed in further research for introduction of the gene to the parental plant and rice breeding for solving food crisis.

• Applied Microscopy
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• v.42 no.2
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• pp.111-114
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• 2012

The scanning electron microscopy is an ideal technique for examining plant surface at high resolution. Most hydrate samples, however, must be fix and dehydrate for observation in the scanning electron microscope. Because the microscopes operate under high vacuum, most specimens, especially biological samples, cannot withstand water removal by the vacuum system without morphological distortion. Cryo-techniques can observe in their original morphology and structure without various artifacts from conventional sample preparation. Rapid cooling is the method of choice for preparing plant samples for scanning electron microscopy in a defined physiological state. As one of cryo-technique, high-pressure freezing allows for fixation of native non-pretreated samples up to $200{\mu}M$ thick and 2 mm wide with minimal or no ice crystal damage for the freezing procedure. In this study, we could design to optimize structural preservation and imaging by comparing cryo-SEM and convention SEM preparation, and observe a fine, well preserved Arabidopsis stem's inner ultrastructure using HPF and cryo-SEM. These results would suggest a useful method of cryo-preparation and cryo-SEM for plant tissues, especially intratubule and vacuole rich structure.

• Journal of Species Research
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• v.2 no.2
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• pp.203-222
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• 2013

The district of Ganghwa-gun in the western coast (Seohae) of the Korea is consisted of several islands, Ganghwa-do, Seokmo-do, Gyodong-do, Jumun-do, Boreum-do, Seogeom-do and Donggeom-do. Since several islands of them were connected to mainland of Korea by bridges, the islands attract a lot of tourists from Seoul, Incheon and Gyeonggi-do areas. Construction of new roads, pensions, resort areas and restaurants provided economical benefits to local residents while natural vegetation of these islands has deteriorated continuously. Before biodiversity of vascular plants in these regions is damaged seriously by human impacts, it is needed to conserve original vegetation of the islands for restoration and monitoring in the future. Therefore, the primary purpose of the study is to clarify the flora of Donggeom-do, belonging to Ganghwa-gun. From the 15 times of field trips to the island, we have collected a number of vascular plants, 512 taxa in total, including 110 families, 329 genera, 449 species, six subspecies, 52 varieties, four forms and one hybrid. Of 512 taxa investigated, we have identified 12 taxa to be endemic to Korea. Six taxa were endangered or rare species which were designated by the Ministry of Environment and Korea Forest Service, respectively. 25 taxa were recognized as floristic regional indicator species designated specially by the Ministry of Environment. Seven species have shown their northern or southern distributional limit ranges, and 29 taxa were recognized as halophytes. The naturalized plants were identified as 55 taxa, and their naturalization ratio was 10.7%.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2006.05a
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• pp.270-273
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• 2006

This paper presents a gas turbine data acquisition and monitoring system using a LabVIEW programming. The developed real-time monitoring system entitled a C-Tune DAS plays an important role to make an analysis of the real-time operation of the gas turbine under maintenance. The LabVIEW based software is divided into three parts according to their original functions; Data acquisition, Data analysis and display, and Data storage. The data acquisition part receives data from a PMS (Plant Management System) server and two cFPs (Compact-Field Point). To verify the validity of the developed system, it is applied to gas turbines in the combined cycle power plant in Korea.

• Plant Biotechnology Reports
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• v.2 no.1
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• pp.75-85
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• 2008

Prune dwarf virus (PDV) is an Ilarvirus systemically infecting almond trees and other Prunus species and spreading through pollen, among other means. We have studied strategies based on coat protein (cp) gene to block PDV replication in host plant cells. A Portuguese isolate of PDV was obtained from infected almond leaves and used to produce the cDNA of the cp gene. Various constructs were prepared based on this sequence, aiming for the transgenic expression of the original or modified PDV coat protein (cpPDVSense and cpPDVMutated) or for the expression of cpPDV RNA (cpPDVAntisense and cpPDVwithout start codon). All constructs were tested in a PDV host model, Nicotiana benthamiana, and extensive molecular characterization and controlled infections were performed on transformants and their progenies. Transgenic plants expressing the coat protein RNA were able to block the proliferation of a PDV isolate sharing only 91% homology with the isolate used for cpPDV cloning, as evaluated by DAS-ELISA on newly developed leaves. With cp expression, the blockage of PDV proliferation in newly developed leaves was only achieved with the construct cpPDV Mutated, where the coat protein has a substitution in the 14th aa residue, with arginine replaced by alanine. This result points to a possible role of the mutated amino acid in the virus ability to replicate and proliferate. This work reveals the possibility of achieving protection against PDV through either coat protein RNA or mutated cp sequence.

• Nuclear Engineering and Technology
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• v.51 no.4
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• pp.1098-1108
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• 2019

Korea's first commercial nuclear power plant at Kori site was permanently shut down in 2017 and is currently in transition stage. Preparatory activities for decommissioning such as historical site assessment, characterization, and dismantling design are being actively carried out for successful D&D (Dismantling and Decontamination) at Kori site. The ultimate goal of decommissioning will be to ensure the safety of workers and residents that may arise during the decommissioning of nuclear facilities and, thereby finally returning the site to its original status in accordance with the release criteria. Upon completion of decommissioning, the resident's safety at a site released will be assessed from the evaluation of dose caused by radionuclides expected to be present or detected at the site. Although the U.S. commercial nuclear power plants with decommissioning experience use different site release criteria, most of them are 0.25 mSv/y. In Korea, both the unrestricted and restricted release criteria have been set to 0.1 mSv/y by the Nuclear Safety and Security Commission. However, since the dose is difficult to measure, measurable concentration guideline levels for residual radionuclides that result in dose equivalent to the site release criteria should be derived. For this derivation, site reuse scenario, selection of potential radionuclides, and systematic methodology should be developed in planning stage of Kori site decommissioning. In this paper, for calculation of a preliminary site-specific Derived Concentration Guideline Levels (DCGLs) for the Nuclear Power Plant site, a novel approach has been developed which can fully reflect practical reuse plans of the Kori site by taking into account multiple site reuse scenarios sequentially, thereby striking a remarkable distinction with conventional approaches which considers only a single site scenario.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.23-23
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• 2022

Rapeseed (Brassica napus L.) is one of the most valuable oilseed crop in the world. It is widely used in various industries, such as food, animal feed, energy and chemical industries. In order to improve the industrial requirements for rapeseed, useful agronomic characteristics (higher yields and disease resistance etc.) and modified oil traits (fatty acid composition and fat content) are important in rapeseed. However, Korea has limiting genetic resources of novel traits in rapeseed. In this research, novel rapeseed mutant genotypes by mutation breeding was developed. The mutant lines were generated by the treatment of the seeds of the original cultivar 'Tamra' with 700 Gy of gamma-ray (⁶⁰Co). Mutants showing varied in flowering time, crude fat content, seed yield and fatty acid content that exhibited stable inheritance of the mutated characteristics from M₅ to M₇ generations were selected. We investigated genetic variation using SNPs identified from GBS analysis in rapeseed mutant lines derived from the gamma-ray, and interactions between the major agronomic and the oil traits. Significantly associated SNP loci were explored along with candidate genes using SNPs obtained by GBS analysis. As a results of association mapping, a total of 322 SNPs were significantly associated with agronomic traits (155 SNPs) and oil traits (167 SNPs). A total of 70 genes were annotated from agronomic characteristics SNPs; among them 7 genes significantly enriched in developmental process, and a total of 70 genes were annotated from crude fat content and fatty acid compositions SNPs; among them, 11genes were significantly enriched in biosynthetic process. These results could be used for the selection of rapeseed cultivar with enhanced qualities and potential economic benefits.

• The Korean Journal of Pesticide Science
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• v.14 no.4
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• pp.433-439
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• 2010

This study was performed to investigate repellent effect of plant essential oils against Bemisia tabaci adult. Among the 20 tested oils, wild mint oil repelled B. tabaci adult significantly (>90%) at a dose of $5{\mu}{\ell}$, but the other oils did not. In dose responses (2, 1, 0.5, $0.1{\mu}{\ell}$) to B. tabaci adult, wild mint oil showed repellent response (77.8~65.7%) significantly with dose-dependent manner. Wild mint oil analyzed by GC/MS revealed that the major components of wild mint oil were menthol (56.5%), menthone (29.0%) and menthyl acetate (14.5%), and the active components responsible for the effective repellency proved to be menthol (77.8%) and menthone (75.8%) when treated the proportion found in original oil. However, menthyl acetate did not show significant repellency. Combined constituents of wild mint oil were showed synergistic effect.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.71-80
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• 2005

Effects of low temperature ($8^{\circ}C$) on the hydraulic conductivity of young roots of a chilling-sensitive (cucumber; Cucumis sativus L.) and a chilling-resistant (figleaf gourd; Cucurbita ficifolia Bouche) crop have been measured at the levels of whole root systems (root hydraulic conductivity, $Lp_r$) and of individual cortical cells (cell hydraulic conductivity, Lp). In figleaf gourd, there was a reduction only in hydrostatic $Lp_r$ but not in osmotic $Lp_r$ suggesting that the activity of water channels was not much affected by low root temperature (LRT)treatment in this species. Changes in cell Lp in response to chilling and recovery were similar asroot level, although they were more intense at the root level. Roots of figleaf gourd recovered better from LRT treatment than those of cucumber. In figleaf gourd, recovery (both at the root and cell level) often resulted in Lp and $Lp_r$ values which were even bigger than the original, i.e. there was an overshoot in hydraulic conductivity. These effects were larger forosmotic (representing the cell-to-cell passage of water) than for hydrostatic $Lp_r$. After a short term (1 d) exposure to $8\;^{\circ}C$ followed by 1 d at $20\;^{\circ}C$, hydrostatic $Lp_r$ of cucumber nearly recovered and that of figleaf gourd still remained higher due to the overshoot. On the contrary, osmotic $Lp_r$ and cell Lp in both species remained high by a factor of 3 as compared to the control, possibly due to an increased activity of water channels. After pre-conditioning of roots at LRT, increased hydraulic conductivitywas completely inhibited by $HgCl_2$ at both the root and cell levels. Different from figleaf gourd, recovery from chilling was not complete in cucumber after longer exposure to LRT. It is concluded that at LRT, both changes in the activity of aquaporins and alterations of root anatomy determine the water uptake in both species. To better understand the aquaporin function in plants under various stress conditions, we examined the transgenic Arabidopsisand tobacco plants that constitutively overexpress ArabidopsisPIP1;4 or PIP2;5 under various abiotic stress conditions. No significant differences in growth rates were found between the transgenic and wild-type plants under favorable growth conditions. By contrast, overexpression of PIP1;4 or PIP2;5 had a negative effect on seed germination and seedling growth under drought stress, whereas it had a positive effect under cold stress and no effect under salt stress. Measurement of water transport by cell pressure probe revealed that these observed phenotypes under different stress conditions were closely correlated with the ability of water transport by each aquaporin in the transgenic plants. Together, our results demonstrate that PIP-type aquaporins play roles in seed germination, seedling growth, and stress response of Arabidopsis and tobacco plants under various stress conditions, and emphasize the importance of a single aquaporin-mediated water transport in these cellular processes.

• Journal of the Korean Society of Marine Environment & Safety
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• v.21 no.6
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• pp.689-695
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• 2015

The offshore plant crews that were commissioned in the commercial startup phase boarded the offshore plant in two shifts until the end of the project. The crews who were hired by the owner side stayed in the original offshore plant during the project. However, most of the offshore plant commissioned members who were dispatched from the shipyard were accommodated in the offshore accommodation barge. For this reason, they were exposed to many accidents since there are a lot of people staying in a small space. This study suggested a method for improving survival rate at offshore accommodation barge in terms of life safety. It is assumed that the fire accident among unfortunate events which take place in the offshore accommodation barge mainly occurred. So, this study analyzed the safety evacuation for offshore plant employees using fire simulation model based on both domestic and international law criteria. In particular, When fire occurs in the offshore accommodation barge, the periodically well trained crews are followed safety evacuation procedure. whereas many employees who have different background such as various occupations, cultural differences, races and nationality can be commissioned with improper evacuation behaviors. As a result, the risk will be greater than normal situation due to these inappropriate behaviors. Therefore, This study analyzed the Required Safe Escape Time (RSET) and Available Safe Escape Time (ASET). Also it was suggested the improvement of structure design and additional arrangement of safety equipment to improve the survival rate of the residents in offshore accommodation barge.