• Title/Summary/Keyword: Oriental lily

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Occurence of Viruses in Lilies (Lilium spp.) in Highland Areas and Their Detection by One-step RT-PCR (고랭지 나리의 바이러스 발생과 RT-PCR에 의한 검정)

  • 김수정;함영일;신관용;류승열;유동림;정효원;최장경
    • Research in Plant Disease
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    • v.7 no.2
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    • pp.80-85
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    • 2001
  • This study was carried out to examine tne incidences of virus diseases in lily plants cultivated in highland areas, and to develop an effective detection method. Viral symptoms on lilies in the highland areas were differentiated into mosaic, crinkle, mottle, stripe and line pattern. The distribution of symptoms on infected plants was 43.8% of mosaic, 29.2% of crinkle, and 10.9% of mottle symptoms. Six viruses such as Lily symptomless vires(LSV), Cucumber mosaic virus (CMV), Lily mottle virus (LMoV), Lily virus X (LVX, Potexvirus), Tabacco mosaic virus (TMV,Tobamovirus), and Tabacco rattle virus (TRV,Tobravirus) were detected from the infected lilies. Infection rate of Lilium oriental (cvs. Casablanca and Marcopolo) was 2~4 times higher than that of L. asiatic (cvs. Solemio and Prato). Virus detection on lilies by one-step RT-PCR (by using reverse transcription and polymerase chain reaction simultaneously) was more rapid rapid and reliable than by the conventional RT-PCR method.

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Effect of Functional Packing Materials on the Maintenance of Freshness of Cut Lily and Cut Rose (기능성 포장재가 백합과 장미의 선도유지에 미치는 효과)

  • Suh, Jeung Keun;Kim, Ji Hee
    • FLOWER RESEARCH JOURNAL
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    • v.18 no.1
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    • pp.57-61
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    • 2010
  • This study was carried out to evaluate the effect of functional packing materials on vase life and quality of Lilium Oriental hybrid 'Le Reve' and Rosa hybrida 'Fire King'. Lily and rose were stored at $5^{\circ}C$ and $20^{\circ}C$ for two days after packing of 25% calcium or 36% wax coated package. When Lilium Oriental hybrid 'Le Reve' was stored in $5^{\circ}C$ after packing of 36% wax coated package, vase life and maintenance of freshness were improved by two days. But when Rosa hybrida 'Fire King' was stored in $5^{\circ}C$ or $20^{\circ}C$after packing of 25% calcium or 36% wax coated package, vase life and maintenance of freshness were not as effective as Lily. In our opinion, functional package seems useful in the storage of cut lily.

Combined Treatment with Gamma Irradiation and Sodium Dichloroisocyanurate to Control Leaf Blight and to Preserve Cut Lilies (절화 백합 잎마름병 방제 및 품질 보존을 위한 감마선 150 Gy와 Sodium Dichloroisocyanurate 병용처리)

  • Koo, Tae-Hoon;Hong, Sung-Jun;Yun, Sung-Chul
    • Research in Plant Disease
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    • v.22 no.2
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    • pp.94-99
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    • 2016
  • For the purpose of practical application of gamma irradiation for export lily without the ionic energy damage, 150 Gy gamma ray was suggested as the safe level on cut lilies, the oriental cultivar Siberia (white) and Sorbonne (pink). The combined treatments of 150 Gy gamma ray and sodium dichloroisocyanurate (NaDCC) at 0, 40, 100, and $200{\mu}g/l$, were tried to control lily leaf blight caused by Botrytis elliptica. Incidences of petal blight on the two oriental lilies at control were 93%-95%, whereas those at the combined treatments of 150 Gy and $100{\mu}g/l$ or higher of NaDCC were 76%-83%. However, severity of petal blight was not reduced by the combined treatments. Gamma irradiation at 150 Gy did not change the cut lily quality such as improve cut lily longevity of vase-life compare with the control. In addition, fresh weight, chlorophyll contents, and the color of pink petals of Sorbonne were not changed by 150 Gy of gamma irradiation. Therefore, we recommend the combined treatment of 150 Gy gamma irradiation and $100{\mu}g/l$ NaDCC for the standard operating procedure on cut lily for export quarantine to control leaf blight.

Detection of Lily Symptomless Virus Using RT-PCR Technique (RT-PCR 기법을 이용한 Lily Symptomless Virus의 검정)

  • Joung, Young-Hee;Jeon, Jae-Heung;Choi, Kyung-Hwa;Kim, Hyun-Soon;Joung, Hyouk
    • Korean Journal Plant Pathology
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    • v.12 no.2
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    • pp.187-190
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    • 1996
  • 백합으로부터 total RNA를 분리하여 LSV 외피단백질 유전자의 551 bp에 해당하는 특정 염기서열을 증폭할 수 있는 primer로 RT-PCR를 수행하였다. 그 결과 Lilium oriental hybrid cvs. Miani, Marco Polo, Casablanca, Le Reve 품종에서 551 bp의 DNA 절편이 증폭되었고 이 절편의 염기서열을 분석한 결과 LSV외피단백질 유전자의 일부임을 확인할 수 있었다. 그러므로 RT-PCR 방법으로, 실험에 사용하 s4품종 모두 LSV에 감염되어 있음을 알 수 있었다.

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Progeny Analysis of Hybrid Lilies Crossed Between Several Genotypes and Tetraploid Oriental-Asiatic Hybrids (체세포 염색체가 배가된 4배체 Oriental-Asiatic(OA) hybrid 유래 종간잡종 백합의 후대검정)

  • Chung, Mi-Young;Chung, Jae-Dong;Van Tuyl, Jaap M.;Lim, Ki-Byung
    • Korean Journal of Breeding Science
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    • v.42 no.1
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    • pp.100-108
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    • 2010
  • This study was carried out to confirm the chromosome constitution and homoeologous recombination of progenies derived from various cross combination using tetraploid OA interspecific hybrid originated from mitotic chromosome doubling. Based on the chromosome analysis of progenies crossed reciprocally, there were only triploid progenies when crossed with diploid Asiatics as male or female parent. While only tetraploid progenies were produced when crossed tetraploid Asiatics or tetraploid OA hybrid with tetraploid OA hybrid, respectively. However, two types of progenies, that is, diploid and triploid plants, were produced from cross combinations between diploid Oriental hybrid and tetraploid OA hybrid. From the GISH analysis of OA hybrid, it was confirmed that diploid $F_1$ OA hybrid was consisted of 24 chromosomes (12 Oriental and 12 Asiatics) showing authentic OA hybrid. On the other hand, it was notified that triploid plants (3x=36) were consisted of 24 Asiatics lily chromosomes and 12 Oriental lily chromosomes by analysis of backcross progenies derived from either $A{\times}OA$ or $OA{\times}A$ crosses. In cross between tetraploid OA and OA, all the progenies were tetraploid with equal number of chromosomes without any homoeologous recombination, i.e. each 24 chromosomes of Oriental and Asiatics. In 2x-4x ($O{\times}OA$) cross combination, some progenies had 2x=24 chromosomes originated from only Oriental hybrid, and other progenies had 3x=36 chromosomes derived from 24 chromosomes of Oriental hybrid and 12 chromosomes of Asiatic hybrid. Only tetraploid Asiatics chromosomes without any Oriental one were produced in all the progenies from 4x-4x ($AA{\times}OA$) cross combination.

A Study on the 'the Diagnosis and Treatment of Bulbus Lilli Syndrome, Malaria Syndrome Characterized by Ulceration, and Poision of Yin-Yang' in Keumkyueyorak (『금궤요약』의 백합병맥증치에 대한 연구)

  • Lee Yun Cheon;Kho Seung Wuk;Jeong Heon Young;Shin Chang Hwan;Lim Hyun Taek
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.18 no.3
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    • pp.670-679
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    • 2004
  • Bulbus lilii syndrome is characterizes by bitter taste, dark urine, indistint and weak pulse for rapture and unstable of mental. It is caused by not be recovered of vital energy after severe illness or mental stress. Bulbus lilli syndrome is similar to depression insomnia but not same. The name of Bulbus lilli syndrome is originated to be cured by Lily Bulb.

Changes in Moisture Content and Quality of Oriental Hybrid Lily (Lilium oriental cv. Siberia) Cut Flowers during Storage at Cold and Dry Condition and Subsequent Exposure to Ambient Temperature (오리엔탈 나리 '시베리아' 절화의 포장내 건식저장 기간별 수분함량과 품질 변화)

  • Lee, Jung-Soo;Rhee, JuHee;Kang, Yun-Im;Choi, Ji Weon
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.23 no.1
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    • pp.27-36
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    • 2017
  • In order to determine the relationship between water content and flower qualities of oriental hybrid lily cv. 'Siberia' cut flower, flowers were subjected to dry and cold storage at $5^{\circ}C$ for 3, 6, and 12 days and subsequently exposed to ambient temperature ($26^{\circ}C$) in bottles with water for up to 16 days. Flowers stored at $22^{\circ}C$ in dry condition for 3 days were used as the control. Changes in fresh weight, moisture content, water balance, flowering stages, osmolality and vase life of cut flowers were observed. Flowers treated with cold and dry storage had higher moisture content compared to control sample. However, this trend was evident only for 3-day cold and dry stored sample during the whole storage period. The fresh weight of cut flowers increased gradually when the samples were transferred to ambient temperature in water bottles and then declined steadily before reaching the peak in between 6-8 days of vase life. However, the changes of fresh weight of control sample were substantially faster than samples pre-treated with cold and dry storage. This was also correlated with the water balance of cut flower as it reached the minus (-) value in 6-8 days of vase life at ambient temperature. Cut lily flowers showed high osmolality values corresponding with the duration of dry storage regardless of low or higher temperature. However, osmolality had no effect on vase life since flower stem absorbed water rapidly at the end of dry storage period. Our vase life results suggest that cold and dry storage of lily cut flowers for a certain period could ensure longer vase life at ambient temperature. It was observed that prolonging the storage period at cold and dry condition for more than a week significantly increased bud abortion, reduced longevity of flowers and reduced the vase life of cut flowers. On the other hand, the shorter cold and dry storage treatment delayed the bud opening and senescence of the flowers, thus, slowering the normal maturation and aging. Results indicated that dry and cold storage at $5^{\circ}C$ for 3 days was effective in maintaining and preserving overall quality and vase life at ambient condition of oriental hybrid lily cut flowers.

Interspecific Pollination of Oriental, Martagon and Trumpet Group as Male Parent in Lilium spp. (Oriental, Martgon 및 Trumpet Group을 화분친으로 사용한 백합의 종간수분)

  • Lee, Ji-Yong;Yoon, Eui-Soo;Lim, Yong-Pyo
    • Journal of Plant Biotechnology
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    • v.31 no.2
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    • pp.95-101
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    • 2004
  • This study was undertaken to study the effect of interspecific pollination of L. longiflorum and L. ${\times}$formolongi as the female parent with Oriental, Martagon and Trumpet group as the male parent by cut-style pollination. In the interspecific pollination of L. longiflorum cv. Gelria and Lorina with Oriental group as the male parent, the corresponding fruits obtained immature embryo were 1, 8, and the mean number of embryo per fruit were 11.0, 3.0, respectively. In the interspecific pollination of L. ${\times}$formolongi cv. Raizan, the corresponding fruits obtained immature embryo were 17, and the mean number of embryo per fruit were 4.0. On the other hand, in the interspecific pollination between L. longiflorum and L. ${\times}$formolongi as the female parent and Martagon and Trumpet group as the male parent, the pollination of L. ${\times}$formolongi as the female parent and L. henryi of Trumpet group as the male parent were obtained only 2 fruits, however no embryo.

Comparison of physiological activities and of useful compounds between new and waste bulbs of different lily (Lilium davidii) varieties

  • Yi, Tae Gyu;Park, Yeri;Yang, Su Jin;Lim, Jung Dae;Park, Sang Un;Park, Kyong Cheul;Park, Nam Il
    • Korean Journal of Agricultural Science
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    • v.43 no.5
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    • pp.734-741
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    • 2016
  • Lily (Lilium davidii) is a high-yielding flowering plant. Besides roses and chrysanthemums, lily bulbs have long been used as food and in oriental medicine. However, the usage and value of cut lily bulbs has not been recognized. A bulb whose yield has been decreased is called a waste bulb, and a large amount of such bulbs is discarded every year. In this study, the functionality of waste bulbs from cut lilies was investigated to explore their potential use as a value-added product. We divided lily bulbs into two groups, one group with six varieties of new bulbs (Medusa, Siberia, Woori Tower, Yelloween, Le Reve, and Morning Star) used for cultivation and the other group with six varieties of waste bulbs (Medusa, Siberia, Woori Tower, Yelloween, Sorbonne, and Sheila). Physiological activities (${\alpha},{\alpha}$-diphenyl-${\beta}$-picrylhydrazyl: DPPH) and 3-ethlbenzthiazolne-6-sulfonic acid (ABTS) radical scavenging capability and tyrosinase inhibiting activity), the amount of total as well as eight individual phenolic compounds (chlorogenic acid, epicatechin, rutin hydrate, p-coumaric acid, kaempferol 3-O-${\beta}$-rutinoside, phloridzin dihydrate, myricetin, and quercetin), and total flavonoid content were measured in the bulbs by high performance liquid chromatography. We detected high amounts of total phenol and total flavonoid as well as high DPPH and ABTS radical scavenging ability. More tyrosinase inhibiting activity was detected in the new bulbs than in the waste bulbs. However, both the new and waste bulbs showed a higher inhibitory activity than the standard (100 ppm ascorbic acid). Although the content of phenolic compounds differed among varieties, under the conditions of the experiment, the most abundant phenolics were epicatechins, followed by chlorogenic acid, and rutins. Overall, the waste bulbs had a higher content of these compounds than the new bulbs. Based on these results, we concluded that bulbs from cut lilies could be used as functional foods in the future and farmers could expect economic gain from the hitherto neglected waste bulbs.

Application of Gamma Irradiation and Its Convergent Treatments on Several Varieties of Oriental Hybrid Lily to Control Leaf Blight (수출용 오리엔탈 백합 품종 잎마름병 방제를 위한 감마선 및 화학 대체제 융복합 처리 효과)

  • Kim, Ji-Hoon;Koo, Tae-Hoon;Hong, Sung-Jun;Yun, Sung-Chul
    • Research in Plant Disease
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    • v.20 no.2
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    • pp.79-86
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    • 2014
  • In order to seek more eco-friend, economic and safer quarantine method than current methyl bromide fumigation, the convergent treatment with 200 Gy of gamma irradiation and several chemicals such as nano-siver particles (NSS), sodium dichloroisocyanurate (NaDCC) was tried on the cuttings of lily in the packing of catonnage box for export. With 6 independent experiments of gamma irradiation on the three lily cultivars, cvs. Siberia, Le reve and Sorbonne, incidence and severity of lily leaf blight was investigated on leaves and petals at 8-d after infection. 200 Gy of gamma irradiation decreased at 13-25% of severity on the leaf of Sorbonne, but it increased at 2-5% of severity on the leaf of Siberia and Le reve. Chemical substitutes such as NSS and NaDCC were not effective to control of lily blight on cuttings. By 200 Gy of gamma irradiation treatment, chlorophyll contents were statistically significantly decreased at 12-d after irradiation and the longevities vaselife of fully open flower of Siberia and Sorbonne were increased at 0.4 to 1.2 days. In addition, the relative fresh weights of the gamma irradiated cuttings were severely dried compared to the non-irradiated control. On the other hands, the symptoms of phyto-toxicity of high dose gamma irradiation at 1 or 2 kGy on cv. Siberia were to be blight at the tip of bloom, bent necks of flower, and delayed the process of flowering.