• Title/Summary/Keyword: Organogenesis

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The functional roles of plant glycogen synthase kinase 3 (GSK3) in plant growth and development (식물의 생장 및 발달과정에서 Glycogen synthase kinase 3 (GSK3) 유전자의 역할)

  • Ryu, Hojin
    • Journal of Plant Biotechnology
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    • v.42 no.1
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    • pp.1-5
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    • 2015

  • The biological roles of glycogen synthase kinase 3 (GSK3) proteins have long been extensively explored in eukaryotic organisms including fungi, animals and plants. This gene family has evolutionary well conserved kinase domain and shares similar phosphorylation properties to their substrate proteins. However, their specific biological roles are surprisingly distinct in different organisms. GSK3s play key role in key regulating the cytoskeleton and metabolic processes in animal systems, but plant GSKs are involved in quite different processes, such as flower development, brassinosteroid signaling, abiotic stresses, and organogenesis. In particular, recent studies have reported the critical multiple functions of BIN2 and its related paralogues plant GSK3s during organogenesis via connecting hormonal or developmental programs. In this review, we outline the recent understanding in the versatile functions related in physiological and biochemical relevance, which are mediated by plant GSK3s in various cellular signaling.

  • https://doi.org/10.5010/JPB.2015.42.1.1 인용 PDF KSCI

Organogenesis by Combined-Dose of Activin A and IGF-1 In Xenopus Presumptive Ectoderm (Xenopus 예정외배엽에서 Activin A와 IGF-1의 복합처리에 의한 기관분화)

  • 정선우;이호선;윤춘식
    • Journal of Life Science
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    • v.9 no.5
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    • pp.504-509
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    • 1999

  • The trend of organogenesis in Xenopus presumptive ectoderm was studied by combined dose of activin A and IGF-1(insulin-like growth factor-1). In reference study of Asashima and his colleagues, the inductive patterns of various organs were reported with activin, the potent mesoderm inducing factor. In present study, the inducing pattern was cleared with combined-dose of concentration 1-100 ng/ml activin A and IGF-1. In addition, the result from single treatment of activin A was compared with former study. As a result, eye was differentiated in 5-20% of explants at 10 and 50 ng/ml concentrative combination of activin A. Otic vesicle was appeared in the entire concentrative combination of IGF-1. Pronephric duct was induced 19-38% of explants at the concentration of activin A 100 ng/ml by adding IGF-1. The comparison of single treatment of activin A was showed some difference in dose-dependent inducing pattern.

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In Vitro Studies on Pinus koraiensis. II. Chromosomal Variation in the Callus (잣나무의 기내배양(器內培養)에 관(關)한 연구(硏究). II. Callus 세포(細胞)의 염색체변이(染色體變異))

  • Kim, Oue Ryong;Park, Young Goo
    • Journal of Korean Society of Forest Science
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    • v.74 no.1
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    • pp.56-60
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    • 1986

  • Excised mature embryos of P. koraiensis were plated on a series of G.D. basic media supplemented with growth regulators as follow; $0.1mg/{\ell}$ NAA(M-1); $0.1mg/{\ell}$ NAA and 2,4-D (M-2); $0.1mg/{\ell}$ 2,4-D and BAP (M-3); $0.1mg/{\ell}$ 2,4-D and kinetin (M-4). Cytological study of the callus showed that thercentage of occurrance of diploid cells was observed 52% in M-3 and 36% in M-4, while that was revealed 29% in M-1 and 17% in M-1. The frequency of diploid cells was increased on the M-3 and M-4 media. The stable chromosome state is a crucial factor for organogenesis. Therefore, it can be inferred that the callus tissues cultured on media supplemented with both auxin and cytokinin have the greatest possibility of organogenesis.

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Multiple shoot induction and callus regeneration in Sarcostemma brevistigma Wight & Arnott, a rare medicinal plant

  • Thomas, T. Dennis;Shankar, Surabhi
    • Plant Biotechnology Reports
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    • v.3 no.1
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    • pp.67-74
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    • 2009

  • An efficient micropropagation protocol based on multiple shoot induction and callus regeneration has been standardized in Sarcostemma brevistigma, a rare medicinal plant. The nodal cuttings were cultured on MS medium supplemented with BA ($0.5-8{\mu}M$) or Kn ($0.5-8{\mu}M$) alone or in combination with NAA ($0.5-1.5{\mu}M$). Maximum multiple shoot induction was observed on MS medium supplemented with $4{\mu}M$ BA. On this medium, 100% cultures responded with an average number of 11.3 shoots per explant. However, the average shoot length was limited to only 0.9 cm on this medium. The addition of $1{\mu}M$ NAA along with $4{\mu}M$ BA gave rise to an average number of 10.9 shoots with an average shoot length of 1.8 cm. Luxuriantly growing callus was obtained on MS medium supplemented with BA ($5{\mu}M$) and 2,4-D ($2{\mu}M$). The callus was subcultured on MS medium supplemented with BA ($2-15{\mu}M$) or Kn ($2-15{\mu}M$) alone or in combination with NAA ($0.5-2{\mu}M$) for shoot organogenesis. Optimum callus regeneration was obtained on MS medium supplemented with $10{\mu}M$ BA and $1{\mu}M$ NAA. On this medium, 100% cultures responded with an average number of 13.4 shoots per culture. The shoots obtained via multiple shoot induction and organogenesis were rooted on half-strength MS medium supplemented with NAA ($1-7{\mu}M$) or IBA ($1-7{\mu}M$). IBA was better than NAA in terms of both the percentage of cultures that responded and the average number of roots per explant. The rooted shoots were successfully transplanted to soil with 86% success. This standardized protocol will help to conserve this rare medicinal plant.

  • https://doi.org/10.1007/s11816-008-0076-1 인용