• 제목/요약/키워드: Organogenesis

검색결과 215건 처리시간 0.046초

국내 딸기 재배품종의 잎절편 배양으로부터 고빈도 식물체 재생 (High Frequency Plant Regeneration from Leaf Explant Cultures of Domestic Cultivated Strawberry (Fragaria x ananassa Duch))

  • 조미애;최규명;고석민;민성란;정화지;유장렬;최필선
    • Journal of Plant Biotechnology
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    • 제32권3호
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    • pp.175-179
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    • 2005
  • 딸기 기내배양으로부터 고빈도 식물체재생 방법을 개발하기 위하여 여봉딸기의 잎과 엽병절편을 0.5 mg/L IBA와 3.2 mg/L kinetin, BAP, zeatin을 각각 조합 첨가한 MS배지에서, 그리고 1주, 2주, 4주동안 암 전처리한 잎 절편을 0.5 mg/L IBA와 3.2 mg/L zeatin이 첨가된 배지에서 6주동안 16시간 광주기 조건으로 배양하였다. 신초 발생은 잎을 adaxial상태로 치상하였을때 절단부위로부터 안토시아닌 색소를 함유한 녹색 캘러스로부터 유도되었고 (57.0%), 엽병의 경우 절단부위로부터 직접 발생되었다(6.3%). 캘러스와 신초발생율은 잎 절편 크기가 클수록 ($1.0{\sim}1.5\;cm^2$) 높았으며, 암 전처리는 그 발생율을 점차 증가시켜 4주동안 암 전처리한 후 16시간 광조건으로 옮겼을 때 가장 높은 발생율 (87.0%)을 얻을 수 있었다. 이러한 shoot발생율 (87%)은 암 전처리를 하지 않은 대조군 (27%) 에 비하여 현저하게 증가된 것이다. 신초를 분리하여 1/2 MS기본배지에 옮겨20동안 배양하였을 때 뿌리가 발생되었으며, 이때 소식물체를 토양에 옮겨 순화시켰다.

Histology of Organogenesis in Sesamum indicum L.

  • Park, Ki-Hoon;Kang, Chul-Hwan;Oh, Myung-Kyu;Choi, Yong-Eui;Seo, Hong-Yul
    • 한국식물생명공학회:학술대회논문집
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    • 한국식물생명공학회 2005년도 춘계학술대회 및 국제심포지움 초록집
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    • pp.161-161
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    • 2005
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Factors Affecting Organogenesis from Mature Cotyledon Explants and Regeneration in Soybean

  • Kim, Young Jin;Park, Tae Il;Kim, Hyun Soon;Park, Ho Ki;Chon, Sang Uk;Yun, Song Joong
    • Journal of Plant Biotechnology
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    • 제6권1호
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    • pp.39-43
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    • 2004
  • A successful, efficient system for multiple shoot induction and plant regeneration of soybean (Glycine max) was established. Four soybean genotypes were compared for organogenic responses on various media cultured under light conditions. The adventitious shoots (98%, 2.6 shoots/cotyledon) directly from one-day-old cotyledon after germination induced by the hormone treatment and its efficiency was higher than any other conditions. The optimal medium for the induction of multiple shoots from cotyledon in Pungsannamulkong(shoot formation rate, 98%), Lx 16 (83%) and IIpumgeomjeongkong(63%) was MS medium supplemented with 2 mg/L BAP, but for Alchankong(75%), MS medium supplemented with 1mg/L zeatin and 1mg/L IAA, 3% sucrose, 4% Phytagel. Higher root induction (88%) was observed from the shoots placed on rooting medium (hormone-free MS basal). Plantlets were transferred onto the same medium supplemented with 1% activated charcoal for further development. With this treatment, regenerated plantlets were obtained within 7-8 weeks (shoot induction for 4 weeks, rooting and shoot elongation for 3-4 weeks).