• Genomics & Informatics
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• v.11 no.4
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• pp.277-281
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• 2013

RNA analysis has become a reliable method of body fluid identification for forensic use. Previously, we developed a combination of four multiplex quantitative PCR (qRT-PCR) probes to discriminate four different body fluids (blood, semen, saliva, and vaginal secretion). While those makers successfully identified most body fluid samples, there were some cases of false positive and negative identification. To improve the accuracy of the identification further, we tried to use multiple markers per body fluid and adopted the NanoString nCounter system instead of a multiplex qRT-PCR system. After measuring tens of RNA markers, we evaluated the accuracy of each marker for body fluid identification. For body fluids, such as blood and semen, each body fluid-specific marker was accurate enough for perfect identification. However, for saliva and vaginal secretion, no single marker was perfect. Thus, we designed a logistic regression model with multiple markers for saliva and vaginal secretion and achieved almost perfect identification. In conclusion, the NanoString nCounter is an efficient platform for measuring multiple RNA markers per body fluid and will be useful for forensic RNA analysis.

• Genomics & Informatics
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• v.14 no.1
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• pp.12-19
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• 2016

Neuropeptides produced from prohormones by selective action of endopeptidases are vital signaling molecules, playing a critical role in a variety of physiological processes, such as addiction, depression, pain, and circadian rhythms. Neuropeptides bind to post-synaptic receptors and elicit cellular effects like classical neurotransmitters. While each neuropeptide could have its own biological function, mass spectrometry (MS) allows for the identification of the precise molecular forms of each peptide without a priori knowledge of the peptide identity and for the quantitation of neuropeptides in different conditions of the samples. MS-based neuropeptidomics approaches have been applied to various animal models and conditions to characterize and quantify novel neuropeptides, as well as known neuropeptides, advancing our understanding of nervous system function over the past decade. Here, we will present an overview of neuropeptides and MS-based neuropeptidomic strategies for the identification and quantitation of neuropeptides.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.20 no.3
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• pp.543-548
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• 2016

Public electronic certificates, as an important means for identification, have been used as the main economic transactions, including banking, e-government, e-commerce. identification. However, damage cases of certificates have been increased by Illegally issued and by hacking practices. Also the users have a difficult in ensuring that their certificates when and where to use. Therefore, the proposed system gives the organization code for the Institutions using OCSP services in advance, the organization code embedded in extensions of OCSP request message structure when institutions ask the validation of certificate to CA(Certificate Authority). Also, OCSP server can extract the organization code from OCSP request message, confirm the institution, and record it in certificate history management table of DB. In this paper, we presented a system that could determine the certificate history check using OCSP service, public electronic certificate validation service, and implemented to prevent and cope immediately with financial incidents.

• Journal of Digital Contents Society
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• v.18 no.2
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• pp.373-382
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• 2017

As the number of scientific and technical contents increases, services that support efficient search of scientific and technical contents are required. When an author's affiliation is used as a keyword, not only the contents produced by the affiliation can be searched, but also the identification rate of the search result using the author and the term as keyword can be improved. Because of the ambiguity and vagueness of the data used as a search keyword, the search result may include false negative or false positive. However, the previous research on the control through identification of the search keyword is mainly focused on the author data and terminology data. In this paper, we propose the algorithm to identify affiliations and experiment with show the experiment with scientific and technological contents held by the Korea Institute of Science and Technology Information.

• Journal of Korean Clinical Nursing Research
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• v.17 no.2
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• pp.215-227
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• 2011

Purpose: This research was done to identify relationships among job stress, fatigue, job satisfaction, and commitment to organization in emergency department nurses. Methods: Research participants were 513 nurses who worked in the emergency department of 17 tertiary centers and 28 secondary hospitals. Data collection for this descriptive correlational study was done from May to July 2010 using self report questionnaires, and analyzed using descriptive statistics, t-test, ANOVA, Pearson correlation coefficient with the SAS 9.2 program. Results: There were statistically significant differences in organizational commitment according to age, religion, total clinical experience, emergency department experience, current position, frequency of night shift, average sleep hours, wage level, numbers of beds in unit, and reason for working. A positive correlation was found between job stress and fatigue, and between job satisfaction and commitment to organization for the emergency department nurses and a negative correlation between job stress and Job satisfaction, and fatigue and Job satisfaction. Conclusion: Results of this research indicate that job stress in emergency department nurses was the principal variable affecting job satisfaction and commitment to organization along with fatigue. Appropriate stress management strategies based on identification of job stress for these nurses should lead to development of improved management of nursing manpower and a reduction in stress.

• Proceedings of the KSR Conference
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• 2008.06a
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• pp.1255-1263
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• 2008

A Safety Case is an railway organization's formal arrangement, through the provision of policies, resources and processes, to ensure the safety of its work activity within safety management system. An effective system helps the organization to identify and manage risks effectively. It allows an organization to demonstrate its capability in achieving its safety objectives and in meeting regulatory requirements. The purpose of this research is to give how to the risk assessments are described within Railway Safety Cases can be prepared and maintained to meet the criteria specified by the upcoming railway regulations. Through this research, we propose the elements of safety management system that include arrangements for the ongoing identification of hazards, assessment of risks and the implementation of necessary control measures.

• Journal of the Korean Society of Marine Environment & Safety
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• v.27 no.3
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• pp.421-428
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• 2021

Identifying which international maritime legal instruments are mandatory or recommendatory is complicated task even for maritime regulatory bodies. Although International Maritime Organization (IMO) had tried to ease the complexity by adopting guidelines on uniform wordings for making reference to other instruments in IMO parent conventions, there has still been some confusion identifying the mandatory status of IMO instruments. The aim of this study was to map out a step-based guideline to resolve the complexity of the mandatory status of IMO instruments to the maximum extent possible. This study reviewed the history of IMO rule-making process to find the root cause of the problem, then analyzed the approaches of regulatory enforcement bodies to check the practices. In conclusion, readers are directed to find such information as to legal status of IMO instruments and an improvement is proposed to enhance the transparency of information sharing for maritime industry to make better informed decisions.

• The Korea Genome Conference
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• 2004.07a
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• pp.46.2-46.2
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• 2004

• The Korea Genome Conference
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• 2005.09a
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• pp.65-65
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• 2005

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2001.10a
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• pp.19-25
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• 2001

At present the identification of vessels is still depending on the OOW (Officer Of Wateh) in VTS (Vessel Traffic Service), which is completed by radar, and also by the combination of VHF radio and VHF direction finder. However, with the development of port transportation and economic, this conventional way of identification can't satisfy more and more request for the information that the VTS needs from the vessels. In such a case, the AIS(Automatic Identification System) precept which is based on STDMA (Self-organized Time Division Multiple Access) technique is put forward by IMO (International Maritime Organization). AIS can automatically provide the information, including own ship's identification, type, position, course, speed, and other information to the appropriately equipped coast station and other ships. At the same time it can also automatically monitor and track the nearby ships similarly fitted with AIS. On the basis of describing the whole comprising and the format of transmission information of AIS, this paper mainly studies the key communication techniques in AIS, such as STDMA protocol, net synchronization and GMSK(Gaussian Minimum Shift Keying)technique, and so on. At last this paper briefly introduces the recommendation decided by IMO on forcing the sea-going ships to fixed with AIS equipments, and it continuos with the unexploited potential of AIS if it applies in VTS.