• Genomics & Informatics
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• 제11권4호
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• pp.277-281
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• 2013

RNA analysis has become a reliable method of body fluid identification for forensic use. Previously, we developed a combination of four multiplex quantitative PCR (qRT-PCR) probes to discriminate four different body fluids (blood, semen, saliva, and vaginal secretion). While those makers successfully identified most body fluid samples, there were some cases of false positive and negative identification. To improve the accuracy of the identification further, we tried to use multiple markers per body fluid and adopted the NanoString nCounter system instead of a multiplex qRT-PCR system. After measuring tens of RNA markers, we evaluated the accuracy of each marker for body fluid identification. For body fluids, such as blood and semen, each body fluid-specific marker was accurate enough for perfect identification. However, for saliva and vaginal secretion, no single marker was perfect. Thus, we designed a logistic regression model with multiple markers for saliva and vaginal secretion and achieved almost perfect identification. In conclusion, the NanoString nCounter is an efficient platform for measuring multiple RNA markers per body fluid and will be useful for forensic RNA analysis.

• Genomics & Informatics
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• 제14권1호
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• pp.12-19
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• 2016

Neuropeptides produced from prohormones by selective action of endopeptidases are vital signaling molecules, playing a critical role in a variety of physiological processes, such as addiction, depression, pain, and circadian rhythms. Neuropeptides bind to post-synaptic receptors and elicit cellular effects like classical neurotransmitters. While each neuropeptide could have its own biological function, mass spectrometry (MS) allows for the identification of the precise molecular forms of each peptide without a priori knowledge of the peptide identity and for the quantitation of neuropeptides in different conditions of the samples. MS-based neuropeptidomics approaches have been applied to various animal models and conditions to characterize and quantify novel neuropeptides, as well as known neuropeptides, advancing our understanding of nervous system function over the past decade. Here, we will present an overview of neuropeptides and MS-based neuropeptidomic strategies for the identification and quantitation of neuropeptides.

• 한국정보통신학회논문지
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• 제20권3호
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• pp.543-548
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• 2016

공인인증서는 본인 확인을 위한 중요한 수단으로 금융거래, 전자정부, 전자상거래 등 주요 경제활동에서 사용되고 있다. 그러나 공인인증서의 부정 발급 및 해킹 등으로 피해 사례가 증가하고 있으나, 사용자는 자신의 공인인증서가 언제, 어디서 사용되었는지 확인하기가 어려운 현실이다. 따라서 제안하는 시스템은 OCSP 서비스 과정에서 공인인증서를 이용하는 기관에 대한 기관코드를 사전에 부여하고, 이용기관에서 공인인증기관에 인증서 검증 요청 시 기관코드를 OCSP Request의 Extensions 부분에 삽입하여 메시지를 전송하며, OCSP 서버는 기관코드를 추출하여 요청기관을 확인하고 해당 결과를 데이터베이스의 이력관리 테이블에 기록하게 된다. 본 논문에서는 인증서 검증 서비스인 OCSP를 사용하여 사용자가 공인인증서 사용이력을 확인할 수 있는 시스템을 제안하고 구현하여 금융사고 등을 방지하고 대처할 수 있도록 한다.

• 디지털콘텐츠학회 논문지
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• 제18권2호
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• pp.373-382
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• 2017

과학기술콘텐츠가 증가함에 따라 과학기술콘텐츠의 효율적인 검색을 지원하는 서비스가 요구되고 있다. 저자의 소속기관명을 키워드로 사용할 경우 한 기관에서 생산된 콘텐츠를 확인할 수 있을 뿐만 아니라 저자, 용어를 키워드로 사용한 검색 결과의 식별율을 향상 시킬 수 있다. 검색 키워드로 사용되는 데이터들의 중의성과 모호성으로 인해 검색 결과에 false negative, false positive가 포함될 수 있으므로 데이터의 식별을 통한 통제는 중요하다. 저자의 소속기관명의 식별을 통한 통제 역시 기관의 이명, 약어 검색을 지원가능하게 하므로 매우 중요하지만 기존의 데이터 식별을 통한 통제에 대한 연구는 저자, 용어에 대한 연구가 주를 이루었다. 본 연구에서는 기관명 식별 알고리즘을 제안하고, 한국과학기술정보연구원에서 보유하고 있는 국내 과학기술콘텐츠들에 대한 데이터를 이용한 실험 결과를 보인다.

• 임상간호연구
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• 제17권2호
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• pp.215-227
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• 2011

Purpose: This research was done to identify relationships among job stress, fatigue, job satisfaction, and commitment to organization in emergency department nurses. Methods: Research participants were 513 nurses who worked in the emergency department of 17 tertiary centers and 28 secondary hospitals. Data collection for this descriptive correlational study was done from May to July 2010 using self report questionnaires, and analyzed using descriptive statistics, t-test, ANOVA, Pearson correlation coefficient with the SAS 9.2 program. Results: There were statistically significant differences in organizational commitment according to age, religion, total clinical experience, emergency department experience, current position, frequency of night shift, average sleep hours, wage level, numbers of beds in unit, and reason for working. A positive correlation was found between job stress and fatigue, and between job satisfaction and commitment to organization for the emergency department nurses and a negative correlation between job stress and Job satisfaction, and fatigue and Job satisfaction. Conclusion: Results of this research indicate that job stress in emergency department nurses was the principal variable affecting job satisfaction and commitment to organization along with fatigue. Appropriate stress management strategies based on identification of job stress for these nurses should lead to development of improved management of nursing manpower and a reduction in stress.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2008년도 춘계학술대회 논문집
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• pp.1255-1263
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• 2008

A Safety Case is an railway organization's formal arrangement, through the provision of policies, resources and processes, to ensure the safety of its work activity within safety management system. An effective system helps the organization to identify and manage risks effectively. It allows an organization to demonstrate its capability in achieving its safety objectives and in meeting regulatory requirements. The purpose of this research is to give how to the risk assessments are described within Railway Safety Cases can be prepared and maintained to meet the criteria specified by the upcoming railway regulations. Through this research, we propose the elements of safety management system that include arrangements for the ongoing identification of hazards, assessment of risks and the implementation of necessary control measures.

• 해양환경안전학회지
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• 제27권3호
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• pp.421-428
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• 2021

Identifying which international maritime legal instruments are mandatory or recommendatory is complicated task even for maritime regulatory bodies. Although International Maritime Organization (IMO) had tried to ease the complexity by adopting guidelines on uniform wordings for making reference to other instruments in IMO parent conventions, there has still been some confusion identifying the mandatory status of IMO instruments. The aim of this study was to map out a step-based guideline to resolve the complexity of the mandatory status of IMO instruments to the maximum extent possible. This study reviewed the history of IMO rule-making process to find the root cause of the problem, then analyzed the approaches of regulatory enforcement bodies to check the practices. In conclusion, readers are directed to find such information as to legal status of IMO instruments and an improvement is proposed to enhance the transparency of information sharing for maritime industry to make better informed decisions.

• 한국유전체학회:학술대회논문집
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• 한국유전체학회 2004년도 The 13th Korea Genome Conference
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• pp.46.2-46.2
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• 2004

• 한국유전체학회:학술대회논문집
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• 한국유전체학회 2005년도 The 14th Korea Genome Conference
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• pp.65-65
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• 2005

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2001년도 Proceeding of KIN-CIN Joint Symposium 2001 on Satellite Navigation/AIS, lntelligence , Computer Based Marine Simulation System and VDR
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• pp.19-25
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• 2001

At present the identification of vessels is still depending on the OOW (Officer Of Wateh) in VTS (Vessel Traffic Service), which is completed by radar, and also by the combination of VHF radio and VHF direction finder. However, with the development of port transportation and economic, this conventional way of identification can't satisfy more and more request for the information that the VTS needs from the vessels. In such a case, the AIS(Automatic Identification System) precept which is based on STDMA (Self-organized Time Division Multiple Access) technique is put forward by IMO (International Maritime Organization). AIS can automatically provide the information, including own ship's identification, type, position, course, speed, and other information to the appropriately equipped coast station and other ships. At the same time it can also automatically monitor and track the nearby ships similarly fitted with AIS. On the basis of describing the whole comprising and the format of transmission information of AIS, this paper mainly studies the key communication techniques in AIS, such as STDMA protocol, net synchronization and GMSK(Gaussian Minimum Shift Keying)technique, and so on. At last this paper briefly introduces the recommendation decided by IMO on forcing the sea-going ships to fixed with AIS equipments, and it continuos with the unexploited potential of AIS if it applies in VTS.