• 제목/요약/키워드: Organization identification

검색결과 347건 처리시간 0.027초

Genome-wide Association Study Identified TIMP2 Genetic Variant with Susceptibility to Osteoarthritis

  • Keam, Bhum-Suk;Hwang, Joo-Yeon;Go, Min-Jin;Heo, Jee-Yeon;Park, Mi-Sun;Lee, Ji-Young;Kim, Nam-Hee;Park, Miey;Oh, Ji-Hee;Kim, Dong-Hyun;Jeong, Jin-Young;Lee, Jong-Young;Han, Bok-Ghee;Lee, Ju-Young
    • Genomics & Informatics
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    • 제9권3호
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    • pp.121-126
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    • 2011
  • Osteoarthritis (OA) is the most common degenerative joint disorder in the elderly population. To identify OA-associated genetic variants and candidate genes, we conducted a genome-wide association study (GWAS). A total 3,793 samples (476 cases: wrist + knee and 3317 controls) from a community-based epidemiological study were genotyped using the Affymetrix SNP 5.0. An intronic SNP (rs4789934) in the TIMP2 (tissue inhibitor of metalloproteinase-2) showed the most significance with OA (odd ratio [OR] = 2.06, 95% confidence interval [CI] = 1.52-2.81, p = $4.01{\times}10^{-6}$). Furthermore, a poly-morphism (rs1352677) in the NKAIN2 ($Na^+/K^+$ transporting ATPase interacting 2) was suggestively associated with OA (OR = 1.43, CI = 1.22-1.66, p = $7.01{\times}10^{-6}$). The present study provides new insights into the identification of genetic predisposing factors for OA.

Mutation Analysis of Synthetic DNA Barcodes in a Fission Yeast Gene Deletion Library by Sanger Sequencing

  • Lee, Minho;Choi, Shin-Jung;Han, Sangjo;Nam, Miyoung;Kim, Dongsup;Kim, Dong-Uk;Hoe, Kwang-Lae
    • Genomics & Informatics
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    • 제16권2호
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    • pp.22-29
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    • 2018
  • Incorporation of unique barcodes into fission yeast gene deletion collections has enabled the identification of gene functions by growth fitness analysis. For fine tuning, it is important to examine barcode sequences, because mutations arise during strain construction. Out of 8,708 barcodes (4,354 strains) covering 88.5% of all 4,919 open reading frames, 7,734 barcodes (88.8%) were validated as high-fidelity to be inserted at the correct positions by Sanger sequencing. Sequence examination of the 7,734 high-fidelity barcodes revealed that 1,039 barcodes (13.4%) deviated from the original design. In total, 1,284 mutations (mutation rate of 16.6%) exist within the 1,039 mutated barcodes, which is comparable to budding yeast (18%). When the type of mutation was considered, substitutions accounted for 845 mutations (10.9%), deletions accounted for 319 mutations (4.1%), and insertions accounted for 121 mutations (1.6%). Peculiarly, the frequency of substitutions (67.6%) was unexpectedly higher than in budding yeast (~28%) and well above the predicted error of Sanger sequencing (~2%), which might have arisen during the solid-phase oligonucleotide synthesis and PCR amplification of the barcodes during strain construction. When the mutation rate was analyzed by position within 20-mer barcodes using the 1,284 mutations from the 7,734 sequenced barcodes, there was no significant difference between up-tags and down-tags at a given position. The mutation frequency at a given position was similar at most positions, ranging from 0.4% (32/7,734) to 1.1% (82/7,734), except at position 1, which was highest (3.1%), as in budding yeast. Together, well-defined barcode sequences, combined with the next-generation sequencing platform, promise to make the fission yeast gene deletion library a powerful tool for understanding gene function.

Using Implementation Science to Advance Cancer Prevention in India

  • Krishnan, Suneeta;Sivaram, Sudha;Anderson, Benjamin O.;Basu, Partha;Belinson, Jerome L;Bhatla, Neerja;D' Cruz, Anil;Dhillon, Preet K.;Gupta, Prakash C.;Joshi, Niranjan;Jhulka, PK;Kailash, Uma;Kapambwe, Sharon;Katoch, Vishwa Mohan;Kaur, Prabhdeep;Kaur, Tanvir;Mathur, Prashant;Prakash, Anshu;Sankaranarayanan, R;Selvam, Jerard M;Seth, Tulika;Shah, Keerti V;Shastri, Surendra;Siddiqi, Maqsood;Srivastava, Anurag;Trimble, Edward;Rajaraman, Preetha;Mehrotra, Ravi
    • Asian Pacific Journal of Cancer Prevention
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    • 제16권9호
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    • pp.3639-3644
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    • 2015
  • Oral, cervical and breast cancers, which are either preventable and/or amenable to early detection and treatment, are the leading causes of cancer-related morbidity and mortality in India. In this paper, we describe implementation science research priorities to catalyze the prevention and control of these cancers in India. Research priorities were organized using a framework based on the implementation science literature and the World Health Organization's definition of health systems. They addressed both community-level as well as health systems-level issues. Community-level or "pull" priorities included the need to identify effective strategies to raise public awareness and understanding of cancer prevention, monitor knowledge levels, and address fear and stigma. Health systems-level or "push" and "infrastructure" priorities included dissemination of evidence-based practices, testing of point-of-care technologies for screening and diagnosis, identification of appropriate service delivery and financing models, and assessment of strategies to enhance the health workforce. Given the extent of available evidence, it is critical that cancer prevention and treatment efforts in India are accelerated. Implementation science research can generate critical insights and evidence to inform this acceleration.

First Report on Tomato bushy stunt virus Infecting Tomato in Korea

  • Kim, Mi-Kyeong;Kwak, Hae-Ryun;Jeong, Seon-Gi;Ko, Sug-Ju;Lee, Su-Heon;Park, Jin-Woo;Kim, Kook-Hyung;Choi, Hong-Soo;Cha, Byeong-Jin
    • The Plant Pathology Journal
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    • 제23권3호
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    • pp.143-150
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    • 2007
  • A new virus-like disease of tomato showing chlorotic spots, malformation and necrosis on leaves, and chlorotic blotching, rings, and necrosis on fruits was observed around Sacheon, Gyeongsangnam-do, Korea in 2004. Host range analysis could not differentiate 4 field isolates collected from tomatoes showing different symptoms but identified them as Tomato bushy stunt virus (TBSV). TBSV-tsf2 isolate induced symptoms in indicator plants similar to those caused by the TBSV-C, -S and -Nf. As the isolate could not systemically infect Chenopodium quinoa, the isolate might belong to the previously described TBSV-S isolate. TBSV-tsf2 isolate caused similar cytological alterations that were similar to that generally caused by previously reported TBSV isolates. TBSV-tsf2 isolate, however, could be considered to belong to new strain of TBSV because masses of small electron-dense patches that were not observed from the previously described TBSV. The complete nucleotide sequences of the genomic RNA of 4739 nt excluding non-translated sequences at both termini have been determined and compared to sequences of other TBSV strains. The complete nucleotide sequence identity among TBSV isolates was 98.9% to 99.7%, and to the other tombusviruses ranged from 80.8% to 94.9%. Comparison of the amino acid sequences all five ORFs with those of other TBSV strains shows a similar genomic organization, and high percentage of amino acid sequence homology with TBSV-Nf than TBSV-S isolate. Since the TBSV symptoms were only observed in Sacheon fields where imported seeds from Japan were planted, the TBSV incidence probably caused by the planting contaminated tomato seeds and thus require more through quarantine procedure to prevent settlement of TBSV in Korea. Altogether, these results support that the Korean isolate of TBSV infecting tomato might be new strain.

교사들의 진화 개념 이해 향상을 위한 논변활동 프로그램 효과 분석 (Analyzing the Effect of Argumentation Program for Improving Teachers' Conceptions of Evolution)

  • 권지은;차희영
    • 한국과학교육학회지
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    • 제35권4호
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    • pp.691-707
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    • 2015
  • 이 연구는 생명과학교사들의 진화개념 향상을 위한 논변활동 프로그램을 개발하고 이를 교사교육에 적용하여 진화개념에 대한 논변활동 특성을 분석함으로써 효과를 알아보고자 하는 것이었다. 이를 위해 먼저 진화를 이해하는데 필요한 주요 개념들을 문헌 조사를 통해 추출하여 '변이', '변이의 유전', '경쟁', '자연선택과 적응', '차등적 생식력', '개체군 내 개체분포의 변화', '대진화'를 주요개념으로 선정하였고, 각각의 개념에 대한 논변활동 질문지를 개발하였다. ADI모형을 변형한 7단계로 이루어진 순환적 논변활동 학습모형도 개발하였다. 총 7회에 대학원 교과교육 수업에 적용하였다. 현직 생명과학교사 4명과 예비 생명과학교사 2명을 대상으로 남, 여 3명씩 두 개의 소집단으로 구성하여 논변활동을 진행하였다. 프로그램이 진행되는 동안 녹음을 통한 담화와 논변글쓰기의 자료를 수집하여 분석하였다. 논변글쓰기는 '설명의 충분성', '설명의 개념적 수준', '증거의 수준', '정당화의 적절성'의 4가지 범주를 기준으로 논변의 질 수준을 분석하여 사전사후에 진화개념의 변화 정도를 확인하였고, 담화분석을 통해 진화주요개념에 대한 논변활동 특성을 분석하였다. 논변글쓰기와 담화를 분석한 결과 개발된 논변활동 모형과 프로그램은 생명과학교사들의 진화개념 이해 향상에 효과적이었다. 이 연구는 논변활동을 통한 교수학습 전략이 생명과학교사교육을 위한 효과적인 진화개념학습 전략이될 수 있음을 보여주었다.

국내의 갈릴레오 탐색구조 지상시스템 개발 방안에 관한 연구 (Study on Development Method for Galileo/SAR Ground System in Korea)

  • 주인원;이상욱;김재훈
    • 한국위성정보통신학회논문지
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    • 제2권1호
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    • pp.35-40
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    • 2007
  • COSPAS-SARSAT 시스템은 위성체와 지상 설비를 이용하여 항공기 또는 선박 등이 조난 시에 탐색구조 (SAR: Search and Rescue) 활동을 도울 수 있도록 조난경보와 위치정보를 제공하는 시스템이다. COSPAS-SARSAT 서비스의 경우, 조난신호 접수에서 구조시작까지 평군 1사간 이상이 소요되고, 위치정확도가 5Km 정도로 범위가 넓은 편이다. 이러한 문제점을 개선하기 위해서 중궤도 위성을 이용한 차세대 탐색구조 시스템 개발이 추진 중에 있으며 EU에서 2011년 FOC(Full Operation Capability)를 목표로 개발중인 갈릴레이 항법위성 프로젝트의 경우 SAR 중계기를 탐재하여 탐색구조 서비스를 제공할 계획에 있다. 갈릴레오 탐색구조(SAR/Galileo)서비스는 수 m급의 위치정확도, 10분 이내의 조난신호 접수에서 구조까지 소요실간. 및 조난자에게 회신링크 서비스 제공 등 보다 향상된 탐색구조 성능을 제공하기 위해 개발 중에 있으므로, 갈릴레오 위성 서비스가 시작되면 탐색구조시스템 체계에 보다 신속하고 정확한 구조가 가능할 것으로 예상된다. 우리나라도 날로 더해가는 다양한 재난에 대한 인명구조를 신속하고 효과적으로 대처하기 위해 차세대 갈릴레오 탐색구조 지상국 도입이 필요하며, 탐색구조 단말기를 포함한 지상국 인프라의 구축 등 갈릴레오 탐색구조 지상시스템 개발 방안에 관한 연구는 매이 시기적절하고 중요한 연구이다. 본 논문은 우리나라가 차세대9 갈릴레오 탐색구조 지상시스템 개발을 위해 필요한 갈릴레오 프로젝트의 참여절차 및 참여전략을 수립하고, 현실적으로 개발이 가능한 개발 범위를 도출하며 개발을 위한 추진체계에 대해서 제안한다.

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국토공간의 효율적 활용을 위한 연안통합관리체계 개선방안에 관한 연구 (A Study on the Improvement of Integrated Coastal Zone Management System for Efficient Use of National Land)

  • 이길재;이주형
    • Spatial Information Research
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    • 제22권5호
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    • pp.99-107
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    • 2014
  • 연안관리 계획은 연안을 이용방향에 따라 4개의 구역으로 분류하도록 하고 있는데, 각 구역별 연안이용행위의 금지 및 지원사항을 정하도록 하고 있다. 연안구역을 설정할 경우 해당지역이 국토이용계획 및 도시계획에 따라 지역 및 지구로 이미 지정되어 있어 추가적인 구역 설정시 이 같은 점이 중요한 검토기준이 되기도 하지만 경우에 따라서는 상충되는 결과를 초래할 수도 있다. 따라서, 연안관리에 있어서의 범위와 이용구역의 설정에 대한 검토 요소를 분석하고, 기존의 지역 지구지정 현황과의 상충문제 해결을 위한 방안을 도출하기 위한 노력이 필요하다. 보다 정확하고 효율적인 연안관리를 위해 해양에서의 해양공간계획과 토지에서 활용되는 도시계획제도와 지적제도를 활용하여 연안지역을 등록 관리할 필요성이 있다. 이를 위해 국토이용계획, 해양공간계획 등을 종합한 계획을 수립할 수 있는 별도의 협의체 기관 검토, 다른 개별법이 정하고 있는 각종 구역 지역의 지정 상충 검토, 연안해역의 범위 설정(3해리 내), 연안등록 방안 및 등록 대상 등을 모색하였다.

간호의 질 평가에 관한 국내 간호연구 분석 (The Analysis of Nursing Research in Korea in relation to the Evaluation of Quality of Nursing)

  • 김남영;장금성;류세앙;김윤민
    • 간호행정학회지
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    • 제9권3호
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    • pp.337-352
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    • 2003
  • Purpose: The purpose of this study is to analyse research trends related to the evaluation of quality of nursing, thereby obtaining basic data relating to the identification of current situation of instrument development of quality of nursing, and of future research orientation and to the development of performance index of nursing organization. Method: The data were collected from June to December, 2002 through the review of total of 32 research papers which had evaluated quality of nursing among published papers in Korea from 1976 to August 2002. The analysis was done in terms of research period, the periodicals in which research papers were published, domains and the approaches of evaluation of nursing quality. The content analysis of lowest-level items amounting was performed using NIC developed by McCloskey & Bulecheck(1998) and consequential indices of quality of nursing developed by Chi(1995). Results: Twenty-three of 32 papers(71.8%) turned out to be published after 1995, indicating surging interest in the evaluation of quality of nursing from the mid 1990s. Also, ten of 12 research papers dealing with subjects' diseases were published after 1995. In content analysis of lowest-level items of the process-oriented evaluation of quality of nursing, the highest nursing intervention was patient education about procedure and treatment; followed in descending order by patient education about disease process, strengthening of communication, managing environment, infection control, admission care, defecation and urination care. In content analysis of lowest-level items of the outcome-oriented evaluation of quality of nursing, items of physical and psychological state, of patient and family satisfaction, of knowledge and home care, of change of patients' state, of addressing nursing issues, and of patient recovery were the outcome indicators in more than 60 percent research papers. Conclusion: The findings provided the foundation for their effective use in nursing practice with comparing and presenting various core evaluation items representing process and outcome domains.

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Identification and Functional Characterization of P159L Mutation in HNF1B in a Family with Maturity-Onset Diabetes of the Young 5 (MODY5)

  • Kim, Eun Ky;Lee, Ji Seon;Cheong, Hae Il;Chung, Sung Soo;Kwak, Soo Heon;Park, Kyong Soo
    • Genomics & Informatics
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    • 제12권4호
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    • pp.240-246
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    • 2014
  • Mutation in HNF1B, the hepatocyte nuclear factor-$1{\beta}$ (HNF-$1{\beta}$) gene, results in maturity-onset diabetes of the young (MODY) 5, which is characterized by gradual impairment of insulin secretion. However, the functional role of HNF-$1{\beta}$ in insulin secretion and glucose metabolism is not fully understood. We identified a family with early-onset diabetes that fulfilled the criteria of MODY. Sanger sequencing revealed that a heterozygous P159L (CCT to CTT in codon 159 in the DNA-binding domain) mutation in HNF1B was segregated according to the affected status. To investigate the functional consequences of this HNF1B mutation, we generated a P159L HNF1B construct. The wild-type and mutant HNF1B constructs were transfected into COS-7 cells in the presence of the promoter sequence of human glucose transporter type 2 (GLUT2). The luciferase reporter assay revealed that P159L HNF1B had decreased transcriptional activity compared to wild-type (p < 0.05). Electrophoretic mobility shift assay showed reduced DNA binding activity of P159L HNF1B. In the MIN6 pancreatic ${\beta}$-cell line, overexpression of the P159L mutant was significantly associated with decreased mRNA levels of GLUT2 compared to wild-type (p < 0.05). However, INS expression was not different between the wild-type and mutant HNF1B constructs. These findings suggests that the impaired insulin secretion in this family with the P159L HNF1B mutation may be related to altered GLUT2 expression in ${\beta}$-cells rather than decreased insulin gene expression. In conclusion, we have identified a Korean family with an HNF1B mutation and characterized its effect on the pathogenesis of diabetes.

관람스포츠에서의 팬시민행동이 구단가치와 지역사회가치에 미치는 영향 (The Effects of Fan Citizenship behavior to Sports team value and Local Community value on Spectator Sports)

  • 이종호;윤대홍;강민석
    • 경영과정보연구
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    • 제30권4호
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    • pp.385-414
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    • 2011
  • 본 연구의 목적을 달성하기 위해 선행연구와 여러 가지 이론을 토대로 설정된 변수들을 연구 모형으로 설정하였다. 독립변수는 팬시민행동에 영향을 미칠 수 있는 개인적 차원과 상호작용적 차원의 요인 구분하였으며, 각 요인들이 팬시민행동이라는 매개변수를 거쳐 구단가치와 지역사회가치에 어떠한 영향을 미치는지 확인하고자 한다. 이에 대한 결과를 요약하면 다음과 같다. 팬 개인적 차원의 특성 중에는 경기력과 연고지가 팬시민행동에 정(+)의 영향을 미치는 것으로 나타났으며, 팬정체성과 선수매력도는 팬시민행동에 유의한 영향을 미치지 못하는 것으로 밝혀졌다. 한편, 팬 상호작용적 차원의 특성 중에는 팀관심동기와 집단주의가 팬시민행동에 정(+)의 영향을 미치며 친사회적가치동기 및 상호의존성은 유의한 영향을 미치지 못하는 것으로 나타났다. 팬시민행동과 결과변수와의 관계를 보면, 팬 시민행동의 경우 구단가치와 지역사회가치에 정(+)의 영향을 미치는 것을 알 수 있었다. 이는 스포츠 마케팅의 효과를 누리는 모든 주체들이 팬의 시민행동을 높일 수 있는 방법에 대체 관심을 가질 필요가 있다는 것이다. 구단의 경우 스포츠 마케팅 효과를 높이기 위해 팬 시민행동을 높일 수 있는 방법에 대한 고려가 필요하며, 이를 바탕으로 강력한 구단 자산을 구축하여 할 필요성이 있다.

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