• Title/Summary/Keyword: Organic culture

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Effect of Chlorella sp. on Improving Antioxidant Activities and Growth Promotion in Organic Soybean Sprout Cultivation (클로렐라 처리에 의한 유기농 콩나물 생육촉진 및 항산화 능력 증진효과)

  • Kim, Min-Jeong;Shim, Chang-Ki;Kim, Yong-Ki;Hong, Sung-Jun;Park, Jong-Ho;Han, Eun-Jung;Jee, Hyeong-Jin;Lee, Sung-Buk;Kim, Seok-Cheol
    • Korean Journal of Organic Agriculture
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    • v.23 no.4
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    • pp.939-950
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    • 2015
  • The purpose of this study was to estimate the growth promoting effects and improvement of antioxidant activity of the soybean sprouts treated with Chlorella sp. culture solution. The soybean sprout treated with 0.1% and 0.2% Chlorella sp. culture solution was significantly increased the length (more than 43.0%), the thickness (more than 0.5~0.7 mm), fresh weight (more than 2.9~3.7 g) compared to non-treated control in vitro. In organic soybean sprouts farm, the 0.2% chlorella culture solution applied to mass culture of soybean sprout and the fresh weight of soybean sprouts increased by more than 25% and the yield was very high as 598.33% compared to untreated control. In addition of sensory test, there is no fishy odor and better crunchy texture and nutty flavor for the treatment soybean sprouts compared to untreated soybean sprouts. Particularly, free-radical scavenging activity (DPPH) and superoxide dismutase activity (SOD) of the soybean sprouts were significantly increased more than 26.1% and 40.4%, respectively by treated with 0.1% and 0.2% Chlorella culture solution. Consequently, the treatment of chlorella culture solution to grow soybean sprouts is also promoting quality and antioxidant activity as well as promoting the growth of sprouts. Therefore, chlorella is considered to be worth as functional materials for high-quality sprouts grown.

Study of Effectiveness of Antimicrobial on Restraining Formation of Biofilms on the Surface of Aluminum (항균제를 이용한 알루미늄 표면에 생물막 형성 억제효과 분석)

  • Park, SangJun;Oh, YoungHwan;Jo, BoYeon;Choi, MiYeon;Hyun, MinWoo;Jeong, JaeHyun;Kim, EuiYong
    • KSBB Journal
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    • v.30 no.2
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    • pp.69-76
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    • 2015
  • The antibacterial activity of a antimicrobial (organic synthetic or organic natural material) on the bacteria (Bacillus megaterium, Arthrobacter oxydans, Micrococcus luteus, Methylobacterium aquaticum) detected in the automobiles showed 99.9% bacteria decrease rate within 30 min of being in contact with the tested bacteria culture. The MIC of the organic synthetic material based antimicrobials and the organic natural material based antimicrobial on the bacteria were 31~500 mg/mL and 8~250 mg/mL, respectively. The bacteria and biofilms were formed on the surface of aluminum after 5 ~8 days in the case of addition of the organic synthetic material based antimicrobial to the MIC values for the tested bacteria culture. On the other hand, there was no proliferation of bacteria and formation of biofilms on the surface of aluminum even after 30 days in the case of addition of the organic natural material based antimicrobial to the MIC values for the tested bacteria culture. As a result, the organic natural material based antimicrobial was confirmed to be more excellent effect of inhibition of bacterial proliferation and restraint of biofilms formation than the organic synthetic material based antimicrobial.

A Study on the Hypoglycemic Effects and the Production Conditions of the Korean Organic Native Rice Cultured by Ganoderma lucidum (영지버섯으로 배양된 한국 유기농 토종 쌀의 생산 조건과 혈당강하 효과에 관한 연구)

  • Yang, Byung-Keun;Lee, Sang-Hwa;Lee, Tae-Geun;Park, Seok-Hwan
    • Korean Journal of Organic Agriculture
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    • v.22 no.3
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    • pp.423-434
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    • 2014
  • This study was conducted to investigate the hypoglycemic effects and the production conditions of the Korean organic native rice cultured by Ganoderma lucidum. The broth culture of Ganoderma lucidum, the innoculation and culture of Ganoderma lucidum to the Korean organic native rice, and the oral administration of the Korean organic native rice cultured by Ganoderma lucidum to the streptozotocin-induced diabetic rats, were carried out. Then, the blood glucose level, the serum total cholesterol and triglyceride, and the activities of alanine aminotransferase and aspartate aminotransferase, were analyzed. The pH 4.5 at $30^{\circ}C$ was the optimal condition of the broth culture of Ganoderma lucidum. When the physical shape and the smell of the produced rice were considered, the optimal conditions to produce the Korean organic native rice cultured by Ganoderma lucidum, were the seeding of Ganoderma lucidum to Korean organic native rice in the rate of 7.5% weight, and the culture period of 9days at $28^{\circ}C$. In streptozotocin-induced diabetic rats, the blood glucose level of the dieted group by the Korean organic native rice cultured by Ganoderma lucidum for 2 weeks, were significantly decreased when compared with the control. And, the levels of the total cholesterol and triglyceride in serum were ranked less than those of the control. Also, in streptozotocin-induced diabetic rats, the activities of alanine aminotransferase and aspartate aminotransferase in the serum of the dieted group by the Korean organic native rice cultured by Ganoderma lucidum for 2 weeks, were lower than those of the control.

Effect of Bacterial and Algal Symbiotic Reaction on the Removal of Organic Carbon in River Ecosystem (하천 생태계에서 유기탄소 기질 제거에 조류와 세균의 공생작용이 미치는 영향)

  • 공석기;도시유끼나까지마
    • Journal of environmental and Sanitary engineering
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    • v.16 no.3
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    • pp.22-27
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    • 2001
  • It have been investigated how algal and bacterial symbiotic reaction influences on removal of organic carbon in river ecosystem. And artificial experimentation apparatus was made for algae'and bacteia'culture as lab scale. Investigating and researching minutely the change of concentration of organic carbon substrate and the change of population density of algae'and of bacteria'with this artificial experimentation apparatus, the next results could be obtained. 1. Successful decrease of DOC(dissolved organic carbon) could not be expected unless algal and bacterial biomass floe was nut formed effectively and unless biosorption was not proceeded effectively in the very culture system in which artificial synthetic wastewater was supplied continuously at constant rate. 2. In conditions of culture liquid of 1335 glucnse mg/L(type 1) and of 267 glucose mg:L(type 2), the algal dominant species was always Chlorella vulgaris in both types in which artificial synthetic wastewater were supplied continuously at constant rate and algae population density was around maximum 107 cells/mL. 3. It was around 108 ~ 107 cells/mL that the population density of heterotrophic bacterium. In culture medium systems type 1 and type 2 in which artificial wastewater were supplied continuously at constant rate, the same density appeared initially when using the population density of Escherichia coli w 3110 as indirect indicator. And this density decreased rapidly till the culturing date 35 days were passed away, while this density increased with gentle slope after same date and then the trend of change at type 2 was more severe than one at type 1. 4. When seeing such a change of population density of Escherichia coli w 3110, the growth of heterotrophic bacterium appeared as survival instinct pattern of broader requirement of nutrient at condition of low concentration of organic carbon substrate than condition of high concentration of same substrate.

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Expressional characteristics of neo-naturalism in contemporary women's fashion (현대 여성 패션에 나타난 신자연주의의 표현 특성)

  • Park, Kyurey;Lee, Younhee
    • The Research Journal of the Costume Culture
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    • v.21 no.5
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    • pp.613-628
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    • 2013
  • This study aims to identify characteristics of neo-naturalism coming from periodical changes in the 21th society, culture based on naturalism and analyze the expressional and design characteristics of neo-naturalism on 2000s. For a research method, this study researched development of naturalism in fashion, and searched digital naturalism and ecology which are design paradigm effecting on neo-naturalism through literature research and preliminary study. Analyzing preliminary study on architecture, interior, fashion about digital naturalism and ecology design, concept of neo-naturalism identified and four expressional characteristics of neo-naturalism was classified, actual examples of neo-naturalism in 21th fashion were extracted and drew design characteristics. The results are as followings. Firstly, naturalism described nature as it is and developed according to the values and needs of the times. Naturalism in fashion showed natrual human body's curve, nature pattern and used natural materical focused on ideal beauty of nature. Secondly, neo-naturalism renews with the foundation of digital culture and ecology design paradigm, and focuses on the flexible possibility to express nature with digital, new media and formative art, and made the artificial nature uniting human-nature-environment as organic whole by ecology design paradigm. Thirdly, design of neo-naturalism divided four characteristics, nature's organic form, combination with the technology, ethical harmony with nature, global local design. The first characteristics of the nature's organic form are expressing silhouette of the nature's organic volume abstractly, the second ones of the combination with the technology are reinterpreting primitive nature contemporary with artificial sensibility of high technology, the third ones of the ethical harmony with nature are showing simple design and high-touch, and the forth ones of global local design are expressing cultural hybrid preserving vernacular design.

Optimization of Organic Compounds and Hydrogen Production in Dark Fermentation using Chlamydomonas reinhardtii (Chlamydomonas reinhardtii를 이용한 암반응에서의 수소 및 유기물 생산 최적화)

  • 공경택;심상준;박대원;김미선;박태현
    • KSBB Journal
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    • v.18 no.1
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    • pp.51-54
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    • 2003
  • The objective of this study was to optimize culture conditions and to produce hydrogen and organic compounds using microalga Chlamydomonas reinhardtii. First of all, C. reinhardtii UTEX 90 was chosen from the three kinds of strains in terms of their hydrogen and organic compound productivity. The optimum $\textrm{CO}_2$ concentration range of C. reinhardtii UTEX 90 was 1to 3%. We tested two medium, which are popular in this microalga culture; Brostol's medium and TAP medium (8). The cell growth in TAP medium was found to be higher than a Brostol's medium. Optimum culture with 3% of $\textrm{CO}_2$ in TAP medium produced the most hydrogen ($0.5\mu$ mol/ mg DCW), though Bristol's medium produced twice as much total organics.

Earthworm Fauna in Citrus Orchards by Legume Cover Crop Culture in Jeju Island (제주 유기 감귤 과수원의 두과피복작물 재배에 따른 지렁이 분포)

  • Park, Jong-Ho;Kim, Yu-Kyoung;Kim, Yong-Ki;Han, Eun-Jung;Shim, Chang-Ki;Byeon, Young-Woong;Kim, Min-Jeong;Kim, Young-Wook
    • Korean Journal of Organic Agriculture
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    • v.27 no.1
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    • pp.77-86
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    • 2019
  • This study was carried out to investigate the effect of legume cover crop culture on earthworm fauna in organic farmland. We compared sod culture with two kinds of legume crops of hairy vetch and crimson clover on organic citrus orchard to natural sod culture and conventional orchards in Jeju Island. We analyzed the soil characteristics and investigated the density of earthworms from the orchards. Organic matter content did not show much difference in soil analysis between organic and conventional orchard. But the biomass of earthworms in organic orchards is 3.8 times to 7.0 times higher than that in conventional cultivated orchards, and individuals of earthworms on organic orchards were 2.3~18 times higher than conventional orchards. The biomass of earthworms on hairy vetch and crimson clover cultivation was 44.8 g, 47.2 g in 2016, and 78.7 g, 31.8 g in 2017, respectively, which were higher than 32.8 g and 9.5 g of those on natural sod cultivation. Through this study, we found that hairy vetch and crimson clover cultivation improve the earthworm occurrence density in the soil on organic citrus orchard.

Microbial composition and diversity of the long term application of organic material in upland soil

  • An, Nan-Hee;Park, Jong-Ho;Han, Eun-Jung;Hong, Sung-Jun;Kim, Yong-Ki;Jee, Hyeong-Jin
    • Korean Journal of Organic Agriculture
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    • v.19 no.spc
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    • pp.190-193
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    • 2011
  • Organic and chemical fertilizer amendments are an important agricultural practice for increasing crop yields. In order to maintain the soil sustainability, it is important to monitor the effects of fertilizer applications on the shift of soil microorganism, which control the cycling of many nutrients in the soils. Here, culture-dependent and culture-independent approaches were used to analyze the soil microorganism and community structure under six fertilization treatments, including green manure, rice straw compost, rapeseed cake, pig mature compost, NPK +pig mature compost, NPK and control. Both organic and chemical fertilizers caused a shift of the cultural microorganism CFUs after treatments. Bacterial CFUs of the organic fertilization treatments were significantly higher than that of chemical fertilization treatments. The DGGE profiles of the bacterial communities of the samples showed that the green manure treatment was a distinct difference in bacterial community, with a greater complexity of the band pattern than other treatments. Cluster analyses based on the DGGE profile showed that rice straw compost and pig mature compost had a similar banding pattern and clustered together firstly. Rapeseed cake, NPK, NPK +pig manure compost and control clustered together in other sub-cluster and clearly distinguished from green manure.

Physicochemical Characteristics and Antioxidant Activities of Organic Strawberries (유기농 딸기의 이화학적 품질특성)

  • Lee, Eun-Ji;Jang, Min-Sun;Kim, Gun-Hee
    • Journal of the Korean Society of Food Culture
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    • v.30 no.6
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    • pp.773-777
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    • 2015
  • Increasing use of organically grown foods has renewed interest in the relationship between agricultural methods and food quality. The objective of this study was to evaluate the physicochemical characteristics and antioxidant activities of organic strawberries. For this study, strawberries were measured in terms of quality characteristics, including weight, length, diameter, hunter color, soluble solid contents (SSC), moisture contents, and pH, as well as antioxidant activities, including DPPH and ABTS radical scavenging activities and total phenol and flavonoid contents. Consequently, conventional strawberries showed higher weight, diameter, and L (lightness) and b (yellowness) values than organic strawberries (p<0.001). Length, moisture contents, pH, a (redness) value, and DPPH and ABTS radical scavenging activities were similar (p<0.05). However, organic strawberries showed higher SSC and total flavonoid and phenol contents than conventional strawberries (p<0.001). These results indicate that there were significant differences between organic and conventional agriculture techniques.

Preparation of Organic Germanium by Yeast Cell (효모를 이용한 유기게르마늄의 제조)

  • 송원종;이상철;오태광
    • Microbiology and Biotechnology Letters
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    • v.23 no.1
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    • pp.87-90
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    • 1995
  • A process of organically bound germanium preparation was developed for healthy food using inorganic germanium adapted Saccharomyces cerevisiae. Adaptations of Saccharomyces cerevisiae against inorganic germanium were successively carried out through stepwise increase of GeO$_{2}$ concentration in order to produce high quantities of germanium bound yeast. Productivity of yeast and quantities of germanium in yeast were obtained 70.2 g/l and 9780 ppm, repectively, when adapted yeast and fed batch culture were used. Germanium taken-up yeast is to be organically bound germanium by evidence of no difference of germanium content after dialysis.

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