• Title/Summary/Keyword: Organic cation

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Antimicrobial, Antioxidant, and Anticoagulation Activities of Salicornia europaea seeds (함초 씨의 항균, 항산화 및 항혈전 활성)

  • Kim, Mi-Sun;Kim, Deuk Hoi;Sohn, Ho-Yong
    • Microbiology and Biotechnology Letters
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    • v.44 no.4
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    • pp.452-460
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    • 2016
  • This study was designed to develop a functional pharma-food using Salicornia europaea (SE). Tiny seeds from the mature SE were collected, and their biological activities were evaluated. The extraction yield of the seed in hot water was found to be 29.6% and the hot water extract (HWE) contained 25.7 mg/g total polyphenol (TP) and 11.5 mg/g total flavonoid (TF), which are similar to those contained in leaf and stem of SE. Among the subsequent organic solvent fractions, the ethylacetate (EA) fraction exhibited the highest content of TP (158.3 mg/g), TF (136.2 mg/g), and total sugar (228.3 mg/g). The EA fraction exhibited broad-range antibacterial activities against gram-positive bacteria, and the butanol fraction exhibited growth inhibitory effect against only Staphylococcus epidermidis. An antioxidation activity assay of the HWE and its fractions showed the EA fraction to have the highest radical scavenging activity with $RC_{50}$ values of 57.0, 29.0, and $28.9{\mu}g/ml$ against DPPH anion, ABTS cation, and nitrite, respectively. The $RC_{50}$ values of vitamin C against DPPH anion, ABTS cation, and nitrite were 10.7, 4.0, and $18.0{\mu}g/ml$, respectively, indicating that the EA fraction of SE has potent antioxidant compounds. In an anticoagulation assay, the EA fraction exhibited a 15-fold extended thrombin time at 5 mg/ml and activated partial thromboplastin time at 7 mg/ml, which are comparable to the activities of aspirin. The HWE and its fractions had no hemolysis activities against human RBCs at up to 1 mg/ml. These results suggest that the EA fraction from SE has a great potential as a new antibacterial and anticoagulation agent.

Influence of Lime and Phosphate Application on Amide and Ureide Nitrogen of Soybean Plants and Soil Microorganisms (석회(石灰)와 인산시용(燐酸施用)이 대두식물체중(大豆植物體中) Amide태(態) 및 Ureide태(態) 질소(窒素)와 토양미생물상(土壤微生物相)의 변화(變化)에 미치는 영향(影響))

  • Ko, Jae-Young;Ryu, In-Soo;Lee, Sang-Kyu;Suh, Jang-Sun
    • Korean Journal of Soil Science and Fertilizer
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    • v.24 no.1
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    • pp.69-76
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    • 1991
  • A pot experiment was conducted to find out the effects of lime and phosphate application on the changes of number of soil microorganisms, indigenous Rhizobium japonicum, nodule formation, and ureide-and amide-N in leaf and stem exudate of soybean plant under uncultivated hillside red earth in very low pH value, organic matter, available phosphate, and cation exchange capacity. The results obtained were summarized as follows : 1. The plant height, stem length, root dry weight and nodule weight were significantly increased with the application of lime and phosphate application than that of control plot. 2. The concentration of amide-N in soybean plant at the 45 days after sowing was obtained as high in order of control>lime> lime+phosphate while the concentration was obtained in order of Iime+phosphate>lime> control at flowering stage 3. However, concentration of ureide-N in the soybean leaf at the 45 days after sowing was obtained as high in order of control>lime>lime+hosphate while reversed concentration was obtained in stem. 4. The number of soil microorgan isms were increased with increase of pH value, available phosphate and soil exchangeable cation. 5. Significantly negative high correlation were obtained with the concentration of Al, Fe in soil and the concentration of amide-and ureide-N in soybean plant at flowering stage while positive correlation was obtained with plant growth and the concentration of ureide-N.

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Impacts of Oyster Shell and Peat Treatments on Soil Properties in Continuous Watermelon Cropping Greenhouse Plots (패화석 및 이탄 처리가 수박 연작지 토양의 특성에 미치는 영향)

  • Ahn, Byung-Koo;Lee, Jin-Ho;Lee, Young-Han
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.4
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    • pp.438-445
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    • 2010
  • Soils in continuous monoculture cropping system generally have a number of physical and chemical problems. Thus, we studied to investigate selected soil properties in continuous watermelon cropping plots with applications of different soil management practices: 1) conventional farming practice (CFP), 2) chemical fertilizer management practice (FMP), the FMP with different amounts, 0.5, 1.0, and 1.5 t $ha^{-1}$, of oyster shell meal application (FMP-OS 0.5, 1.0, and 1.5 t $ha^{-1}$), and 3) the FMP with different amounts, 2.0, 3.0, and 4.0 t $ha^{-1}$, of peat application (FMP-PT 2.0, 3.0, and 4.0 t $ha^{-1}$) and also to evaluate watermelon quality. Soil pH slightly increased only in the FMP-OS 1.5 t $ha^{-1}$ plot, while it was not changed or decreased a little in other plots. The contents of soil organic matter (SOM) expectedly increased in the FMP-PT plots, whereas it markedly decreased in the FMP-OS plots. The concentrations of exchangeable cations, $Ca^{2+}$, $Mg^{2+}$, and $K^+$, in soils were mostly dropped down in most of the FMP and FMP-PT plots. Otherwise, the exchangeable $Ca^{2+}$ concentration increased a bit in the FMP-OS plots. Also, the concentrations of water-soluble anions, $NO_3^-$, $Cl^-$, ${SO_4}^{2-}$, and ${PO_4}^{3-}$, in soils mostly declined in all the plots applied with the different management practices during the study years. Due to the cation and anion decreases, the electrical conductivity (EC) values in the soils were greatly reduced in the plots. Thus, the soil management practices applied, especially oyster shell meal and peat treatments, might be useful to control soil conditions. However, watermelon quality, such as sugar content and fruit weight, would not be associated with the soil management practices applied.

Soil Physical-chemical Characteristics on Indigenous Plant and Naturalized Plant of Coastal Sand Dune on Central-western Coastal Area, Korea (중부 서해안 해안사구 자생식물과 귀화식물 군락의 토양특성 비교)

  • Kim, Chan-Beom;Son, Yowhan;Bae, Yeong-Tae;Park, Ki-Hyung;Youn, Ho-Joong;Kim, Kyongha;Lee, Chang-Woo
    • Journal of Korean Society of Forest Science
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    • v.102 no.3
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    • pp.323-330
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    • 2013
  • This study was conducted to know the effect of major physical-chemical characteristics of soil to be in the area of indigenous and naturalized plant in the sand dune of the western coastal area of Korea. The study was performed on the sand dunes distributed across Taean-gun and Buan-gun and the study period was from April to October in 2010. Sixty nine of $5m{\times}5m$ study plots were installed and the distribution of plants were investigated. We measured the soil characteristics including soil pH, organic matter, total nitrogen, available $P_2O_5$, soil cation exchange capacity, exchangeable cation, EC and NaCl. As a result, soil texture was classified as sand, in case of average pH, NaCl, and EC of soil to be in the area of indigenous herbaceous plant were 7.77, 0.03% and $0.52ds.m^{-1}$, indigenous woody plant of the mean pH, NaCl and EC were 7.31, 0.01%, $0.23ds.m^{-1}$. In case of naturalized herbaceous plant of the mean soil pH, NaCl and EC were 7.12, 0.01%, $0.29ds.m^{-1}$, naturalized woody plant of the mean soil pH, NaCl and EC were 7.34, 0.01%, $0.20ds.m^{-1}$ respectively. On average, naturalize plants showed in low salinity concentration than indigenous plants.

Micromorphological Characteristics of Soil with Different Patent Materials (모재별 토양의 미세형태 특성)

  • Zhang, Yong-Seon;Jung, Seog-Jae;Kim, Sun-Kwan;Park, Chang-Jin;Jung, Yeon-Tae
    • Korean Journal of Soil Science and Fertilizer
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    • v.37 no.5
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    • pp.293-303
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    • 2004
  • This experiment was conducted to investigate the direction or orientation of clay particle movement in argillic horizons (Bt) for clarifying the soil classification of soils. Soil samples were collected from 22 soil series containing Bt horizons. Physical and chemical characteristics and mineral and chemical compositions of clay in the soils were analyzed. Micoromorphological characteristics of the Bt horizons were also investigated with thin sections of the natural undisturbed and oriented soil samples. Average clay content in the Bt horizons was 28% and 1.33 times higher comparing to that in the surface layer. Soil pH was higher, but cation exchange capacity (CEC) and organic matter content were lower in Bt horizon than those in the surface layer. There was an evidence of clay accumulation in Bt horizons of all soil series examined except Bangog series. Although there was an increase of clay content in the horizons in Bangog series, the clay was not originated from illuviation process. The translocation of clay was in the order of an 2:1 expandable clay minerals > 2:1 non-expandable clay minerals > 1:1 clay minerals. The illuvial substances in argillic horizon were composed with clay, amorphous iron and opaque mineral. The micoromorphological features of Bt horizon were void coating, channel infilling and grain coating. There was an apparent boundary between clay coating and the groundmass in residuum and colluvium, but Bt horizon of alluvium was composed of a skew plane amputated by the physical operation.

Residue and adsorptive capacity of paraquat in orchard soils (우리나라 과수원 토양의 Paraquat 잔류와 흡착능)

  • Chun, Jae-Chul;Kim, Sung-Eun;Park, Nam-Il;Lim, Sung-Jin
    • The Korean Journal of Pesticide Science
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    • v.2 no.3
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    • pp.90-95
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    • 1998
  • Soil residues of paraquat (1,1-dimethyl-4,4-dipyridinium dichloride) were determined in apple, pear, grape, and peach orchards for which 15 sites each were selected randomly from the corresponding large-scale production area throughout the country. Strong adsorption capacity measured using wheat bioassay (paraquat concentration required to reduce 50% root growth of wheat, SAC-WB) was also investigated on the orchard soils and the paraquat residue level was calculated against total SAC-WB values (SAC-WB value + paraquat residue). Average bound residue of paraquat on the 60 sites was 6.9 ppm, while paraquat residue in apple orchard reached 20.2 ppm which was the highest among the orchards and was almost double as compared with those in the other three orchards. Loosely bound residue of paraquat determined on the bound residue high top five soils occurred less than 0.5 ppm detection limit. Average SAC-WB value was 276.1 ppm and there were no any correlations between the SAC-WB value and clay content, organic matter content, and cation exchange capacity of the orchard soils. Paraquat residue level of the orchard soils against total SAC-WB recorded 2.43%.

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Influence of animal wastes on the soil fertility parameters and the growth of corn (Zea mays L.) (축산폐기물(畜産廢棄物)의 이용(利用)에 관(關)한 연구(硏究) : 가축분뇨(家畜糞尿)가 토양화학성(土壤化學性) 및 옥수수 생육(生育)에 미치는 영향(影響))

  • Kim, Jeong-Je;Hong, Byong-Ju;Goh, Yong-Gyun
    • Korean Journal of Soil Science and Fertilizer
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    • v.24 no.2
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    • pp.137-143
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    • 1991
  • This research was conducted to investigate the treatment effects of the experimental product of an oxidatively treated animal wastes such as feces of cow and pig on the growth and yield of corn, soil fertility parameters, nutrient uptake by corn, and in situ dry matter digestibility. The results are summarized as follows. (1) Growth of corn was favored by treatment of the experimental products as compared to the control. Highest yields were obtained at treatment levels of 2,000 and 2,500kg/10a for the experimental products derived from cow and pig feces, respectively. (2) The contents of soil organic matter were increased 7-41% and 4-60% with treatments of experimental products from cow and pig feces, respectively, as compared to the control. The available soil phosphorus levels were increased significantly with the treatments. Treatment of product from the cow feces resulted in a slight increase of the potassium adsorption ratio (KAR). (3) No significant difference was observed in uptake of total nitrogen and phosphorus between the treatments and the control. Uptake of cation by corn was in the order of $K_2O$ >CaO>MgO. (4) In situ dry matter digestibility ratio was increased with Incubation time. However, no significant difference in digestibility was detected for the corn samples produced by treating different levels of the experimental products.

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Estimation of the Planting Environment of Planted Areas around Sudokwon Landfill Site (수도권매립지 주변 식재지의 식재기반 평가)

  • Park, Heon;Yoon, Yong-Han;Kim, Won-Tae;Lee, Hyun-Jong;Park, Bong-Ju
    • Journal of Forest and Environmental Science
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    • v.24 no.1
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    • pp.35-39
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    • 2008
  • The present study was conducted to analyze the soil environment of planted areas around Sudokwon Landfill Site, and drew conclusions as follows. Among the physical properties of soil, bulk density ranged between 1.11~1.59 $g{\cdot}cm^{-3}$, which was higher than the average bulk density (1.05 $g{\cdot}cm^{-3}$) of the B layer of forest soil in Gyeonggido, Korea. This is probably because of treading by heavy equipment used to prepare the ground for planting, and measures should be taken to improve bulk density for the growth of the root system of trees. Among the chemical properties of soils, the organic matter content was only 1/5~1/10 of 30 $g{\cdot}kg^{-1}$, the adequate level for landscape planting. In addition, cation exchange capacity (CEC) ranged between 1~3 $cmol{\cdot}kg^{-1}$, which was much lower than 6 $cmol{\cdot}kg^{-1}$, the least capacity for landscape planting. Therefore, these problems need to be solved.

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Studies on ammonium adsorption by and desorption from various soils (I) -Langmuir adsorption isotherm of ammonium (토양별(土壤別) 암모늄의 흡착(吸着) 및 탈착(脫着)에 관(關)한 연구(硏究) (I) -암모늄의 Langmuir 등온흡착(等溫吸着))

  • Shim, Sang-Chil;Kim, Kwang-Rai;Kim, Moo-Sung;Park, Hoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.10 no.1
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    • pp.1-6
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    • 1977
  • Fifteen soils including volcanic ash, acid sulfate and degraded saline soils were investigated for Languir adsorption isotherm of ammonium using $NH_4H_2PO_4$. The results are as follows. Languir adsorption maxima of ammonium (LAMA) ranged from 2.4me/100g soil to 12.3 and the average was 5.3. Initial concentration of 30 to 60 or 40 to 80 ppm(as N) appears to be suitable for LAMA measurement. There were two LAMA in some soils. Difference between adsorption constants (bonding energy) was mostly greater than that between LAMA. LAMA ranged from 9.4% to 72% of cation exchange capacity and average was 47%. It did not show any clear tendency with CEC, pH, organic matter content, base saturation percent, P, K, Ca, Mg, Na and Si. Except volcanic ash soils which were grouped into two groups according to ammonium adsorption LAMA was significantly (r=0.951 at 1%) correlated with adsorption at 200ppm. This single concentration seems suitable for LAMA measurement. Probable mechanism of ammonium adsorption was discussed, in which the associated anions were combined with iron and aluminum and then ammonium was bound to phosphorus. Applicability of Langmuir adsorption isotherm model to the soils under field condition was also discussed.

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Survey on the General Physical and Chemical Characteristics of Apple Orchard Soils (사과원토양(園土壤)의 일반이화학적성질(一般理化學的性質)에 관(關)한 조사연구(調査硏究))

  • Lee, Mahn Jung
    • Korean Journal of Soil Science and Fertilizer
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    • v.6 no.4
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    • pp.205-211
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    • 1973
  • General physical and chemical characteristics of eleven apple orchard soils derived from granite, granodiorite, diorite, arkose sandstone and shale were investigated in relation to soil profile. The results are summerized as follows: 1. Soils were mostly sandy and showed pH 4.35 to 6.75, 1.14 to 5.58 of organic matter, 0.065 to 0.209% of nitrogen. All properties decreased with depth. 2. Total exchangeable bases of surface soils were comparablly low (4.89 to 17.81me/100g, Ca>Mg>K>Na). Cation exchange capacity ranged from 7.74 to 21.72 mg/100g and base saturation percentage from 22.52 to 94.62%. 3. Phosphorus content of surface soil was very low (less than 35.5 mg/100g). The contents of potassium (7.2-79.2 mg/100g), available copper (1.0-16.9 ppm) and water soluble boron (0.18-0.72 ppm) were high in surface soil but low with depth. The content of manganese showed clear difference with pH, that is, at pH 4 to 6 water soluble manganese ranged from 0.42 to 17.8 ppm and exchangeable manganese from 9.82 to 66.38 ppm but above pH 6 the water soluble was less than 0.70 ppm and the exchangeable less than 22.25 ppm.

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