• The Journal of the Korea Contents Association
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• v.14 no.9
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• pp.218-226
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• 2014

China has become a G-2 Superpower country as result of rapid economic growth through the reform and opening-up in these days. It expressed its confidence by helding a few big international events, such as Bejing Olympic Games. Shanghai Expo. Though most Chinese people give their agreement and support to the government, there are serious, ill-concealed political problems behind the curtain in China. As a multi-ethnic nation, China has repeated experience of fragmentation and integration of various ethnic groups through her long history. In spite of top-level political leaders of communist party always concern the issues of separatism very sensitively, China has suffered occasional bloodshed terrorism because of erupting ethnic and religious conflicts. Especially there are many disruption and fear due to the bloodshed and terrorism by a minority race-seperatists of Uighur tribe in Xinjiang province. The Uighur in Xinjiang province is very different from major Chinese Han ethnically and culturally. Although Chinese government has presented various policies in order to suppress the Uighus's desire for seperation, they have not been able to offer a fundamental, satisfying solution. Because we, Korean people face the expansion of possibility of conflicts, such as increasing of foreign workers, multi-cultural families and extremists. At this point, I believe we can learn valuable lessons from Chinese experiences for anti-terrorism.

• Journal of Microbiology and Biotechnology
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• v.20 no.12
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• pp.1630-1636
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• 2010

During the fermentation process of Saccharomyces cerevisiae, yeast cells must rapidly respond to a wide variety of external stresses in order to survive the constantly changing environment, including ethanol stress. The accumulation of ethanol can severely inhibit cell growth activity and productivity. Thus, the response to changing ethanol concentrations is one of the most important stress reactions in S. cerevisiae and worthy of thorough investigation. Therefore, this study examined the relationship between ethanol tolerance in S. cerevisiae and a unique protein called alcohol sensitive RING/PHD finger 1 protein (Asr1p). A real-time PCR showed that upon exposure to 8% ethanol, the expression of Asr1 was continuously enhanced, reaching a peak 2 h after stimulation. This result was confirmed by monitoring the fluorescence levels using a strain with a green fluorescent protein tagged to the C-terminal of Asr1p. The fluorescent microscopy also revealed a change in the subcellular localization before and after stimulation. Furthermore, the disruption of the Asr1 gene resulted in hypersensitivity on the medium containing ethanol, when compared with the wild-type strain. Thus, when taken together, the present results suggest that Asr1 is involved in the response to ethanol stress in the yeast S. cerevisiae.

• Journal of Microbiology and Biotechnology
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• v.27 no.2
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• pp.335-341
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• 2017

The complexity of the bacterial recombination system is a barrier for the construction of bacterial mutants for the further functional investigation of specific genes. Several protocols have been developed to inactivate genes from the genus Pseudomonas. Those protocols are complicated and time-consuming and mostly do not enable easy construction of multiple knock-ins/outs. The current study describes a single and double crossover-recombination system using an optimized vector-free allele-exchange protocol for gene disruption and gene replacement in a single species of the family Pseudomonadaceae. The protocol is based on self-ligation (circularization) for the DNA cassette which has been obtained by overlapping polymerase chain reaction (Fusion-PCR), and carries an antibiotic resistance cassette flanked by homologous internal regions of the target locus. To establish the reproducibility of the approach, three different chromosomal genes (ncRNA31, rpoN, rpoS) were knocked-out from the root-associative bacterium Pseudomonas stutzeri A1501. The results showed that the P. stutzeri A1501 mutants, which are free of any plasmid backbone, could be obtained via a single or double crossover recombination. In order to optimize this protocol, three key factors that were found to have great effect on the efficiency of the homologous recombination were further investigated. Moreover, the modified protocol does not require further cloning steps, and it enables the construction of multiple gene knock-in/out mutants sequentially. This work provides a simple and rapid mutagenesis strategy for genome editing in P. stutzeri, which may also be applicable for other gram-negative bacteria.

• Journal of Korean Academy of Nursing
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• v.29 no.1
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• pp.61-71
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• 1999

Sleep is a necessity for survival. Disruption of sleep leads to numerous adverse physiological and psychological consequences. These could be particularly undesirable for older patients, who are subject to many additional factors. But there is limited research related to hospitalized elderly in Korea. The purpose of the study is to explore sleep patterns and disturbing factors of before and after hospitalization, in order to present basic information regarding elderly sleep to develop nursing intervention. The sample consisted of 32 elderly men and women between the ages of n and 87 years. Data collection was done from September to November 1997. Measures of sleep patterns and related factors were obtained from self-reported sleep questionnaires. Analysis of data was done by use of t-test, paired t-test, ANOVA, and Pearson Correlation Coefficient. The results of this study were summarized as follows : 1. In comparision between before and after admission of their sleep pattern, “sleep onset” tends to be delayed and nocturnal sleep time was significantly reduced. So, hospitalized elderly reported less total sleep time than before admission. 2. Regarding the sleep disturbing factors, medication(hypnotics ; 37.5%), physiological factor (snoring ; 59.4%) environmental factor (pillow ; 78.1%), emotional factor(anxiety related to disease ; 37.5%), and illness factor(fatigue ; 34.7%) were reported. 3. Significant differences in gender were found. Men had more disturbances in sleep than women owing to difficulty in falling a sleep and lack of nocturnal sleep. Women consumed more sleep inducing drugs. Significant increase was reported in napping during the day with increasing age. 4. Significant differences between good sleepers and poor sleepers were found for the following variables : nocturnal sleep time, total sleep time, bed time, sleep onset latency time, sleep latency time after nocturnal awakening, time spent in bed upon arousal, environmental factors, and emotional factors. In conclusion, it was found that the quantity and quality of sleep were significantly altered in hospitalized elderly, but adequate strategies for better sleep were not practiced. Further research is needed to develop Intervention strategies to promote sleep and to prevent sleep problems.

• Proceedings of the Microbiological Society of Korea Conference
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• 2006.05a
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• pp.105-107
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• 2006

Two kinds of nucleoside hydrolases (NHs) encoded by rih1 and rih2 were cloned from Corynebacterium ammoniagenes using deoD- and gsk-defective Escherichia coli. Sequence analysis revealed that NH 1 was a protein of 337 aa with a deduced molecular mass of 35,892 Da, whereas NH 2 consisted of 308 aa with a calculated molecular mass of 32,310 Da. Experiments with crude extracts of IPTG-induced E. coli CGSC 6885(pTNU23) and 6885(pTNI12) indicated that the Rihl enzyme could catalyse the hydrolysis of uridine and cytidine and showed pyrimidine-specific ribonucleoside hydrolase activity. Rih2 was able to hydrolyse both purine and pyrimidine ribonucleosides with the following order of activity-inosine>adenosine>uridine>guanosine>xanthosine>cytidine-and was classified in the non-specific NHs family. rih1 and rih2 deletion mutants displayed a decrease in cell growth on minimal medium supplemented with pyrimidine and purine/pyrimidine nucleosides, respectively, compared with the wild-type strain. Growth of each mutant was substantially complemented by introducing rih1 and rih2, respectively. Furthermore, disruption of both rih1 and rih2 led to the inability of the mutant to utilize purine and pyrimidine nucleosides as sole carbon source on minimal medium. These results indicated that rih1 and rih2 play major roles in the salvage pathways of nucleosides in this micro-organism.

• Journal of the Korean Institute of Gas
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• v.12 no.3
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• pp.75-80
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• 2008

According to the Integrated Notice on City Gas Safety Management Standards, materials for bedding and foundation which are around the pipe should be sands or fine grade soil without large particle that is more than 19 mm size. However, sands are mostly used at gas pipeline construction sites and this causes a shortage of sands and an increase of construction costs. It even causes the disruption of natural environment. In order to improve the standards of backfill material, we have researched regulations in other countries and investigated the pipeline construction sites to survey the present state of backfilling. We also have studied what the bedding and foundation materials affect on buried gas pipelines. Lastly, we have suggested suitable materials for bedding and foundation besides sands. We are sure this paper help the government amend the Notice about backfill materials.

• Structural Monitoring and Maintenance
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• v.2 no.3
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• pp.283-300
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• 2015

Visual condition inspections remain paramount to assessing the current deterioration status of a bridge and assigning remediation or maintenance tasks so as to ensure the ongoing serviceability of the structure. However, in recent years, there has been an increasing backlog of maintenance activities. Existing research reveals that this is attributable to the labour-intensive, subjective and disruptive nature of the current bridge inspection method. Current processes ultimately require lane closures, traffic guidance schemes and inspection equipment. This not only increases the whole-of-life costs of the bridge, but also increases the risk to the travelling public as issues affecting the structural integrity may go unaddressed. As a tool for bridge condition inspections, Unmanned Aerial Vehicles (UAVs) or, drones, offer considerable potential, allowing a bridge to be visually assessed without the need for inspectors to walk across the deck or utilise under-bridge inspection units. With current inspection processes placing additional strain on the existing bridge maintenance resources, the technology has the potential to significantly reduce the overall inspection costs and disruption caused to the travelling public. In addition to this, the use of automated aerial image capture enables engineers to better understand a situation through the 3D spatial context offered by UAV systems. However, the use of UAV for bridge inspection involves a number of critical issues to be resolved, including stability and accuracy of control, and safety to people. SLAM (Simultaneous Localisation and Mapping) is a technique that could be used by a UAV to build a map of the bridge underneath, while simultaneously determining its location on the constructed map. While there are considerable economic and risk-related benefits created through introducing entirely new ways of inspecting bridges and visualising information, there also remain hindrances to the wider deployment of UAVs. This study is to provide a context for use of UAVs for conducting visual bridge inspections, in addition to addressing the obstacles that are required to be overcome in order for the technology to be integrated into current practice.

• Structural Engineering and Mechanics
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• v.55 no.6
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• pp.1261-1278
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• 2015

Due to their low intrinsic damping, stay cables in cable-stayed bridges have often exhibited unanticipated and excessive vibrations which result in increasing maintenance frequency and disruption to normal operations of the entire bridges. Mitigation of undesired cable vibration can be achieved by attaching an external damping device near the anchorage. High Damping Rubber (HDR) dampers have many advantages such as compact size, better aesthetics, easy maintenance, temperature stability, and cost benefits; therefore, they have been widely used to increase cable damping. Although a single damper has been shown to reduce cable vibrations, it is not the most effective method due to geometric constraints. This paper proposes the use of two HDR dampers to improve effectiveness and robustness in suppressing cable vibration. Oscillation parameters of the cable-dampers system were investigated in detail by modeling the stay cable as a taut string and each HDR damper as complex-valued impedance and by using an analytical formulation of the complex eigenvalue problem. The problem of two HDR dampers arbitrarily located along a cable is solved and the solution is discussed. Asymptotic formulas to calculate the damping ratios of the cable with two HDR dampers installed near the anchorage(s) are proposed and compared with the exact solutions. Further, a design example is presented in order to justify the methodology. The results of this study show that when the two HDR dampers are installed close to each other on the same end of the cable, some interaction between the dampers leads to reduced damping ratio. When the dampers are on the opposite ends of the cable, they are effective in increasing damping ratio and can provide better vibration reduction to multiple modes.

• International Journal of Industrial Entomology and Biomaterials
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• v.37 no.2
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• pp.36-42
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• 2018

Recently, silk sericin has attracted attention because of its unique properties as a biomaterial, including its UV resistance, moisturizing effect on skin, and wound-healing effect. Therefore, the preparation of sericin in various forms such as gel, film, fiber, and sponge is studied for cosmetic and biomedical applications, and the effect of the preparation conditions on the structure and properties of sericin forms is examined to maximize its performance. In this study, silk sericin films were prepared under different preparation conditions and heat-treated at high temperatures ($100-250^{\circ}C$) to examine the effect of heat treatment on the film structure. The order of the crystallinity index of the untreated sericin film is as follows: F25 (sericin film cast from formic acid) > WE25 (ethanol treated sericin film cast from water at $250^{\circ}C$) > W25 (sericin film cast from water at $250^{\circ}C$) > W100 (sericin film cast from water at $100^{\circ}C$). As the heat-treatment temperature was increased, the color of the sericin films changed gradually from colorless to yellow, brown, and black depending on the temperature. The crystallinity of the sericin film changed after the heat treatment, depending on the preparation condition. Whereas a sericin film cast from formic acid (F25) started to lose its crystallinity at $200^{\circ}C$, thus undergoing the highest loss of crystallinity among the sericin films studied, the rest (W25, WE25, and W100) showed a decrease in crystallinity at $250^{\circ}C$, owing to the disruption of the ${\beta}$-sheet crystallites due to heat.

• Membrane Journal
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• v.32 no.1
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• pp.23-32
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• 2022

Bacteria grow biofilm on various surface such as separation membrane, food packaging film and biomedical device. Growth of biofilm is associated with the formation of a complex structure of exopolysaccharides. Effect of antibacterial effect reduce drastically once the biofilm developed due to the difficulties in mass transport of antimicrobial agent. In order to enhance the antibacterial activity, surface of the membrane is modified, coated or immobilized with functional materials with biocidal properties. One of the idea is to introduce positive charge on the membrane surface by the presence of quaternary ammonium group which might displace divalent metal ion such as magnesium or calcium present in the bacteria cell wall. Efficacy of cell membrane disruption depends on the mobility of the agents available directly on the surface environment. In this review, various biocidal agents like quaternary ammonium group, helamine or zwitter ion containing membrane are discussed.