• Ocean and Polar Research
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• v.27 no.2
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• pp.189-195
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• 2005

Several models predict large and potentially abrupt ocean circulation changes due to anthropogenic greenhouse-gas emissions. These circulation changes drive-in the models-considerable oceanic oxygen trend. A sound estimate of the observed oxygen trends can hence be a powerful tool to constrain predictions of future changes in oceanic deepwater formation, heat and carbon dioxide uptake. Estimating decadal scale oxygen trends is, however, a nontrivial task and previous studies have come to contradicting conclusions. One key potential problem is that changes in the historical observation network might introduce considerable errors. Here we estimate the likely magnitude of these errors for a subset of the available observations in the Southern Ocean. We test three common data analysis methods south of Australia and focus on the decadal-scale trends between the 1970's and the 1990's. Specifically, we estimate errors due to sparsely sampled observations using a known signal (the time invariant, temporally averaged, World Ocean Atlas 2001) as a negative control. The crossover analysis and the objective analysis methods are for less prone to spatial sampling location biases than the area averaging method. Subject to numerous caveats, we find that errors due to sparse sampling for the area averaging method are on the order of several micro-moles $kg^{-1}$. for the crossover and the objective analysis method, these errors are much smaller. For the analyzed example, the biases due to changes in the spatial design of the historical observation network are relatively small compared to the tends predicted by many model simulations. This raises the possibility to use historic oxygen trends to constrain model simulations, even in sparsely sampled ocean basins.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.2
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• pp.128-135
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• 1984

Gamadi, a native rice cultivar from Nepal in which the panicle remains enclosed within its flag leaf sheath upto maturity, was crossed with different genetic marker testers of 12 linkage groups in order to analyze its linkage relationship. The results obtained from the experiment were summarized as follows: Normal segregations of all the genetic marker genes used in this experiment viz Cl, wx and Pla of linkage group I, Pn, Rd and Pub of linkage group III, and lg, g, Ps, gh, Hla, la, nl, bl, be and gl of linkage groups II, IV, V, VI, VII, VIII, IX, X, XI, and XII respectively confirmed the previous results, and also strongly indicated that the genetic constituent of the Gamadi and marker testers is same. 'Gamadiness' (the panicle enclosing character) was controlled by two complementary dominant genes with the segregation ratio of 9 Gamadi to 7 normal panicle-exserting types. These genes have been temporarily proposed as G-a and G-b for gamadiness. G-a gene was found to be linked with the neckleaf gene (nl) of linkage group Ⅸ with the crossover value of 0.3733$\pm$0.027. G-b gene appeared to be associated with the brittle culm gene (bc) of the linkage group XI with the crossover value of 0.2725$\pm$0.061.TEX>0.061.

• Journal of the Korean Electrochemical Society
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• v.8 no.1
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• pp.6-11
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• 2005

Direct borohydrides fuel cell (DBFC) was emerged to complement the problem of DMFC's low performance and methanol crossover to the cathode and to apply the fuel cell to portable and mobile devices. In this study, the characteristics of novel catalysts was tested to establish the electrode preparation process of DBFC. Pt black and carbon supported-Pt by paste method were used as the cathode catalysts. Pt black, carbon supported-Au and $AB_5$ alloy were used as the anode catalysts. The characteristics of the electrodes were analyzed by XRD, SEM, EDS. The performance test of single cell using the electrodes were carried out in order to evaluate the electrode performance. In the result, the maximum power output was obtained as 366 mW/mg when using Pt/C as anode and cathode catalysts.

• Journal of Microbiology and Biotechnology
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• v.27 no.2
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• pp.335-341
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• 2017

The complexity of the bacterial recombination system is a barrier for the construction of bacterial mutants for the further functional investigation of specific genes. Several protocols have been developed to inactivate genes from the genus Pseudomonas. Those protocols are complicated and time-consuming and mostly do not enable easy construction of multiple knock-ins/outs. The current study describes a single and double crossover-recombination system using an optimized vector-free allele-exchange protocol for gene disruption and gene replacement in a single species of the family Pseudomonadaceae. The protocol is based on self-ligation (circularization) for the DNA cassette which has been obtained by overlapping polymerase chain reaction (Fusion-PCR), and carries an antibiotic resistance cassette flanked by homologous internal regions of the target locus. To establish the reproducibility of the approach, three different chromosomal genes (ncRNA31, rpoN, rpoS) were knocked-out from the root-associative bacterium Pseudomonas stutzeri A1501. The results showed that the P. stutzeri A1501 mutants, which are free of any plasmid backbone, could be obtained via a single or double crossover recombination. In order to optimize this protocol, three key factors that were found to have great effect on the efficiency of the homologous recombination were further investigated. Moreover, the modified protocol does not require further cloning steps, and it enables the construction of multiple gene knock-in/out mutants sequentially. This work provides a simple and rapid mutagenesis strategy for genome editing in P. stutzeri, which may also be applicable for other gram-negative bacteria.

• Journal of the Korea Society of Computer and Information
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• v.24 no.11
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• pp.179-185
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• 2019

In this paper, We purpose the basic data for the success of effective CPR using mirror in order to increase the quality of chest compression during CPR. The subject of this study was an experimental study based on a randomized crossover design of 28 people who completed the BLS Health Care Provider, and collected data were analyzed by SPSS Ver. 23.0 for Win statistics program. As the research methods, depth, speed, compression to relaxation ratio, arm angle and easiness during the chest compression were measured. Taken together, the results of this study showed that using a mirror-based chest compression method for chest compressions in adult CPR could make chest compressions easier, in addition, the quality of breast compression was improved by improving the posture of the rescuers, such as the average depth of compression, compression to relaxation ratio, and arm angle. However, it is necessary to confirm the feasibility of clinical application through additional studies on various environmental factors and job groups for mirror-based chest compression method.

• Archives of Pharmacal Research
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• v.21 no.6
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• pp.779-781
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• 1998

The aim of this study is to provide evidence on the aphrodisiac property of Eurycoma longifolia Jack. An electric grid was used as an obstruction in the electrical copula tion cage in order to determine how much an aversive stimulus the sexually naive male rat for both the treated with E. longifolia Jack and control groups were willing to overcome to reach the estrous receptive female in the goal cage. The intensity of the grid current was maintained at 0.12mA and this was the intensity in which the male rats in the control group failed to crossover to reach the goal cage. Results showed that E. longifolia Jack continued to enhance and also maintain a high level of both the total number of successful crossovers, mountings, intromissions and ejaculations during the 9-12 th week observation period. In conclusion, these results further enhanced and strenghtened the aphrodisiac property of E.longifolia Jack.

• Journal of Aerospace System Engineering
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• v.1 no.2
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• pp.21-26
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• 2007

DMFC(Direct Methanol Fuel Cell) has been considered as an attractive option to produce electric power in many application. In this study, in order to estimate the effects of the methanol concentration, wind velocity and temperature on the performance of DMFC, a physical prototype of DMFC was designed and manufactured, and the stack voltage of DMFC was measured during the operation of DMFC. Expecially, the experimental results showed that a low stack temperature, a low wind velocity and an excess methanol concentration lead to the increase of the time to reach the maximum stack voltage.

• Journal of The Korean Astronomical Society
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• v.37 no.5
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• pp.471-476
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• 2004

We propose an analytical model to estimate the influence of a merger on the thermal SZ effect. Following observations we distinguish between subsonic and transonic mergers. Using analytical velocity fields and the Bernoulli equation we calculate the excess pressure around a moving subcluster for an incompressible subsonic gas. Positive excess around the stagnation point and negative excess on the side of the subcluster lead to characteristic signatures in the SZ map, of the order of $10\%$ compared to the unperturbed signal. For a transonic merger we calculate the change in the thermal spectral SZ function, resulting from bow shock accelerated electrons. The merger shock compression factor determines the power law tail of the new non-thermal electron population and is directly related to a shift in the crossover frequency. This shift is typically a few percent towards higher frequencies.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.20 no.41
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• pp.1-14
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• 1997

This study proposes a genetic algorithm using Pr(equation omitted)fer number for the traveling salesman problem(PNGATSP). Nearest neighbor nodes are mixed with randomly selected nodes at the stage of generating initial solutions. Proposed PNGATSP adopts a few ideas which are different from traditional genetic algorithms. For instance, an exponential fitness function and elitism are used and Pr(equation omitted)fer number is used for encoding TSP. Genetic operators are selected by experiments, which make a good solution among four combinations of conventional genetic operators and new genetic operators. For respective combinations, robust set of parameters is determined by the experimental designing approach. The feature of Pr(equation omitted)fer number code for TSP and the search power of GA using Pr(equation omitted)fer number is analysed. The best is a combination of OX(order crossover) and swap, which is superior to the other experimented combinations of genetic operators by 1.0%∼12.8% deviation.

• International Journal of Fuzzy Logic and Intelligent Systems
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• v.2 no.2
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• pp.115-121
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• 2002

This paper presents an evolutionary design of digital IIR filters using the genetic algorithm (GA) with modified genetic operators and real-valued encoding. Conventional digital IIR filter design methods involve algebraic transformations of the transfer function of an analog low-pass filter (LPF) that satisfies prescribed filter specifications. Other types of frequency-selective digital fillers as high-pass (HPF), band-pass (BPF), and band-stop (BSF) filters are obtained by appropriate transformations of a prototype low-pass filter. In the GA-based digital IIR filter design scheme, filter coefficients are represented as a set of real-valued genes in a chromosome. Each chromosome represents the structure and weights of an individual filter. GA directly finds the coefficients of the desired filter transfer function through genetic search fur given filter specifications of minimum filter order. Crossover and mutation operators are selected to ensure the stability of resulting IIR filters. Other types of filters can be found independently from the filter specifications, not from algebraic transformations.