• 생약학회지
• /
• 제33권3호통권130호
• /
• pp.238-244
• /
• 2002

The extracts from stem and fruit of Cactus (Opuntia ficus-indica var. saboten Makino) were applied to confirm the antiatherosclerotic effects in subacute alcoholic hyperlipidemic rats. It was observed that several indications of hyperlipidemia were prevented or changed by the treatments of the extracts but activities of hepatic HMG-CoA reductase and alcohol dehydrogenase were not affected, which suggest that the extracts may promote the fecal excretion and delay the absorption of alcohol in hyperlipidemic rats.

• Journal of Applied Biological Chemistry
• /
• 제50권4호
• /
• pp.221-226
• /
• 2007

The high molecular-weight mucilage extracted and purified from cactus fruit of Opuntia ficus-indica var. Saboten was degraded by the cell-free culture filtrate of a fungus isolated from soil. TLC analysis of the polymeric mucilage after incubation with the fungal culture filtrate confirmed its degradation. When the degradation products were tested for their qualitative reactions with ninhydrin and phenol-sulfuric acid, only phenol-sulfuric acid gave positive development, and ninhydrin did not show any observable color reaction. This coloring reaction suggested the presence of a carbohydrate without an amino group within the mucilage. Analyses by HPLC and liquid gel permeation chromatography on sephadex G-100 also provided additional information on degradation of the mucilage by the fungal culture filtrate. The sequences of ITS-5.8S rDNA from the fungal isolate that was cultivated for the preparation of mucilage-degrading enzyme showed 99% similarity to those of Pestalotiopsis aquatica.

• 대한한의학방제학회지
• /
• 제17권2호
• /
• pp.163-174
• /
• 2009

Reactive oxygen species(ROS) can induce hepatotoxicity and trigger apoptosis in the liver. In this study, we investigated the sulfated polysaccharide A-1 from Opuntia ficus-indica against alcoholic oxidative stress in human liver Hep G2 cell. An antioxidant substance A-1 obtained from the enzymatic extract of Opuntia ficus-indica fruit was purified by DEAE-cellulose ion exchange and sephadex G-100 gel permeation chromatography. The purification yield and molecular weight were 14.3% and 1.8 KDa, respectively. The A-1 predominately contained arabinose, galactose, rhamnose and also sulfate group. The structure of A-1 was investigated by periodate oxidation, FT-IR spectroscopy, $^1H$-NMR spectroscopy. The A-1 mainly composed of alternating unit of ${\rightarrow}4$)-$\alpha$-L- Rapp-2-$SO_3^-$-$\alpha$-L-Galp-($1{\rightarrow}$ and branched linkage of $\beta$-D-Arbp- ($5{\rightarrow}$. The antioxidative activity was measured using the SOD, CAT activity and GSH assay, respectively. The expression of Nrf2 protein was analyzed by western blotting. The viable cell count analyzed by autofluorescence. Oxidative stress induced by ethanol(1 M) were dramatically reduced by A-1 treatment. A-1 also prevented cell death induced by oxidative stress. It also increased expression Nrf2 protein level. We concluded that sulfated polysaccharide A-1 from Opuntia ficus-indica effectively protect Hep G2 liver cell from alcoholic oxidative stress.

• 한국수산과학회지
• /
• 제52권4호
• /
• pp.358-365
• /
• 2019

This study investigated the effects of dietary cactus Opuntia ficus-indica stem and fruit extract on the growth, flesh quality, lysozyme activity, and histological changes of growing Korean rockfish Sebastes schlegeli. Three replicates of fish (152 g/fish) were fed one of the following diets: containing 0 additions (control); 0.1, 0.5, or 1.0% cactus stem powder; or 1.0% fruit extract for 11 weeks. Growth performance did not differ significantly among treatments, including survival, final weight, feed efficiency, and daily feed intake. The experimental diets did not affect the proximate and fatty acid compositions, plasma biochemistry, or dorsal muscle texture of the fish. However, the plasma lysozyme activity of the fish fed the diet containing 0.1% cactus stem was significantly higher than that of the fish fed the control diet. These fish had variously sized lipid vacuoles in the liver tissue compared with the control. Distinct mucosal folds and mucus-secreting goblet cells developed in the fish fed the diet containing 1% cactus stem compared with the other dietary groups. These results suggest that feeding growing Korean rockfish cactus stem might increase the plasma lysozyme activity and induce histological changes in the gastrointestinal tract that might be related to digestion.

• 생약학회지
• /
• 제32권4호통권127호
• /
• pp.330-337
• /
• 2001

Prickly pear(Opuntia ficus-indica var. saboten Makino, Cactaceae) is a tropical or subtropical plant, which is widely used as folk medicine for burned wound, edema and indigestion. Screening on the biological properties of the fruits(OFS-Fr) and stems(OFS-St) of the plant was carried out to prove the pharmacological significance. By hot plate and acetic acid-inducing writhing methods, significant analgesic effects of OFS-Fr and OFS-St were found in mice and anti-edemic effect was observed in carrageenin-induced inflammatory rats. However, the extracts showed no significant actions on central and autonomic nervous system and blood circulatory system, which imply no toxic effects to animal.

• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 2007년도 Proceedings of The Convention of The Korean Society of Applied Pharmacology
• /
• pp.115-121
• /
• 2007

Opuntia ficus-indica var. saboten (Cactaceae) is widely cultivated in Jeju Island (South Korea) for use in the manufacture of health foods such as tea, jam and juice. Its fruit and stem have been traditionally used as oriental folk medicine to treat diabetes, hypertension, asthma, burns, edema and indigestion. During the search for naturally occurring antioxidative neuroprotective agents, it was found that the ethyl acetate fraction of the stems and fruits of Opuntia ficus-indica var. saboten exhibited potent antioxidant effect. Using a chromatographic fractionation method, several constituents were isolated from this fraction. Among isolates, KYS50182 showed the most potent in vitro and in vivo neuroprotective activities, suggesting that the flavonoid can serve as a lead structure for the development of neuroprotective agents by providing neuroprotection against oxidative and focal ischemic neuronal injuries.

• 한국식품영양학회지
• /
• 제29권5호
• /
• pp.663-669
• /
• 2016

This study was conducted to analyze the nutrient compositions of stem and fruit of Opuntia ficus-indica var. saboten (OF) and Opuntia humifusa (OH). The results of the two-way analysis of variance test indicated that the parts, varieties and parts*varieties had influence on nutrient content except crude protein, soluble dietary fiber, Fe, Se, I, vitamin E, niacin and vitamin C. Moisture and crude ash content was higher in stem than in fruit, while crude lipid and insoluble dietary content was higher in fruit than in stem. Mineral content revealed that K (1,313.67 mg% in OF, 1,351.38 mg% in OH) was the highest in fruit and Ca (5,146.29 mg% in OF, 1,388.19 mg% in OH) was the highest in stem. Vitamin C was the most abundant vitamin in the fruit of OF (199.98 mg%) and OH (187.12 mg%). Polyunsaturated fatty acid was the highest among fatty acids (66.9~70.1%), with higher content in the stem (753.89 mg%) than fruit (578.01 mg%) in OF, while higher in the fruit (1,093.63 mg%) than stem (475.07 mg%) in OH. Moisture, crude protein, Mg, Se and riboflavin was higher in OF than OH; whereas, crude lipid, insoluble dietary fiber, total dietary fiber and monounsaturated fatty acid were higher in OH than OF. These results indicated that OF and OH could be a good food source for Ca, K and Vitamin C. Also, different nutrient content by parts and varieties, can be helpful in choice of parts or varieties for consumer purposes.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2000년도 춘계총회 및 학술대회
• /
• pp.177.1-177.1
• /
• 2000

• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
• /
• pp.187-187
• /
• 1998

The objective was to verify biological activities of fruit and stem of prickly pear(Opuntia ficus indica L. var, saboten Makino). We have determined inhibitory activities on enzymes, such as dopamine ${\beta}$-hydroxylase(DBH), monoamme oxidase A and B(MAO-A, B), and antioxidant activity, in vitro. We purchased dried stem powder and lyophilized fruit powder of prickly pear from CheJu Island, and prepared the extracts with 80% of methanol. The fruit extract showed stronger inhibitory effects on MAO-A and -B and antioxidant activity compared. to the stem extract, on fractionation with hexane, ethyl acetate, butanol and water. Both the stem and the fruit extracts with ethyl acetate showed stronger enzyme inhibitory and antioxidant activities than other extracts. Now we are isolating active principles from both ethyl acetate extracts.

• 한국식품저장유통학회지
• /
• 제23권5호
• /
• pp.711-717
• /
• 2016

본 연구에서는 우리나라에서 재배되고 있는 손바닥선인장인 백년초(Opuntia ficus-indica var. saboten)와 천년초(Opuntia humifusa) 열매의 외관 및 성분을 비교하였다. 시료 간 외형을 장축, 단축, 중량의 항목으로 구분하여 측정, 비교하였으며, 색도를 측정하여 외관을 비교하였다. 크기와 무게는 천년초 열매가 백년초 열매에 비해 작았으나, 단축 대비 장축의 비가 백년초 열매는 1.63, 천년초 열매는 1.90으로 천년초 열매가 백년초 열매에 비해 크기가 작은 대신 좀 더 길쭉한 모양을 가지는 것을 확인하였다. 색도의 경우 L값과 b값은 천년초 열매가, a값은 백년초 열매가 높았는데, 열매시료간의 ${\Delta}E$값은 19.80으로 나타나 색도 차이가 크게 나타나는 것으로 나타났다. 일반성분의 함량은 백년초 열매가 탄수화물 함량이 높은 반면 조회분 함량은 천년초 열매가 높게 나타났으며, 조단백질 함량과 조지방 함량은 유사하게 나타났다. 식이섬유 함량은 백년초와 천년초 열매 모두 유사한 수준으로 높은 함량(52.7~53.4%)을 가지고 있는 것으로 나타났는데, 식이섬유 함량이 높다고 알려진 알로에와 해조류보다도 높은 수준으로 나타났다. 무기질 함량은 두 시료에서 K과 Ca순으로 높게 나타났는데, 한국인이 주로 섭취하는 과일의 K과 Ca함량에 비해 높은 함유량을 나타내었고, Ca이 많이 함유되어 있다고 보고된 멸치와 비슷한 Ca함량을 나타내었다. 구성아미노산의 함량은 전체 합이 천년초 열매가 백년초 열매에 비해 높았으나 필수 아미노산의 비율은 백년초 열매가 천년초 열매에 비해 소폭 높았고, 모든 시료에서 glutamic acid의 함량이 가장 높은 특징을 보였다. 유리 아미노산의 경우 시료별 특성이 달랐는데, 백년초 열매의 경우 tyrosine의 함량이 높고 천년초 열매에 존재하지 않는 taurine이 존재한 반면, 천년초 열매의 경우 glutamic acid의 함량이 압도적으로 높은 것을 확인할 수 있었으며, 모든 시료에서 GABA가 검출되었다. 본 연구결과 백년초와 천년초 열매가 외관특성 및 성분에 차이를 보이는 것을 확인할 수 있었는데, 손바닥선인장 열매의 가공제품 개발 시 종에 따른 외관과 성분함량 차이를 고려할 필요가 있을 것으로 판단되었고 특히 손바닥선인장 열매의 경우 식이섬유와 Ca의 함량의 높아 이를 이용한 기능성식품의 제조에 이용될 수 있을 것으로 사료되었다.