• Title/Summary/Keyword: Optimum Mixing Ratio

Search Result 429, Processing Time 0.031 seconds

Development of HRP-modified Carbon Composite Biosensor and Electrochemical Analysis of H2O2 (Horseradish peroxidase가 변성된 탄소복합 바이오센서 개발 및 전기화학적 H2O2분석)

  • Park, Deog-Su
    • Journal of the Korean Chemical Society
    • /
    • v.56 no.5
    • /
    • pp.571-576
    • /
    • 2012
  • A sol-gel derived carbon composite electrodes (CCEs) were fabricated by mixing horseradish peroxidase (HRP), sol of tetraethoxysilane (TESO), and graphite powder. The HRP solution was added to the sol solution of TEOS, and then graphite powder was added to this mixture. The resulting carbon ceramic network effectively encapsulated HRP and shows a catalytic reduction starting at -0.2 V for $H_2O_2$. The optimum conditions for $H_2O_2$determination have been characterized with respect to the enzyme loading ratio and pH. The linear range and detection limit of $H_2O_2$ detection were from 0.2 mM to 2.2 mM and 0.035 mM, respectively. The common electroactive interferences such as ascorbic acid, acetaminophene, and uric acid were not affected upon the response to $H_2O_2$ at the HRP biosensor due to low detection potential.

Research on Characteristics of Multifunctional Soil Binder Based on Polyacrylamide (폴리아크릴아마이드를 기반으로 하는 다기능성 토양안정제의 특성에 관한 연구)

  • Kim, Jin Kyung;Kim, Dae Ho;Joo, Sang Hyun;Lee, Myung Cheon
    • Applied Chemistry for Engineering
    • /
    • v.29 no.2
    • /
    • pp.155-161
    • /
    • 2018
  • For the efficient recovering of collapsed sloped soil, using a soil binder that can support the soil strongly and help the growth of plants is very important. The soil binder should also have functions of recovering the soil ecologically as well as be environmental friendly materials. In this research, optimum values of the water content and permeability and direct shear strength were searched by adding the water absorbent and coagulant into the soil binder. The polyacrylamide (PAM) with various anionic strength, super absorbent polymer (SAP) and cellulose ether (CE) were used as a soil binder, water absorbent and coagulant, respectively. Effects of the soil binder on the characteristics of soil were observed by changing the mixing ratio of PAM, SAP and CE. Experimental results showed that the soil binder increased the direct shear strength tens of times and the water content around two times, whereas decreased the water permeability. Also, the addition of CE to increase the coagulation of SAP increased more of the direct shear strength and water content.

A Study on the Preparation of High Purity Silicic Acid by Solvent Extraction from Sodium Silicate (규산 나트륨으로부터 유기 용매 추출에 의한 고순도 규산 제조에 관한 연구)

  • Rho, Jae-Seong;Hong, Seong-Su;Chung, Hung-Ho
    • Applied Chemistry for Engineering
    • /
    • v.7 no.4
    • /
    • pp.802-808
    • /
    • 1996
  • Silicic acid(SA) was extracted with organic solvents from aqueous sodium silicate solutions acidified with dilute sulfuric acid. Tetrahydrofuran(THF), isopropyl alcohol and acetone were used as organic solvents. The extraction degree of Si and the removal efficiency of Na is determined according to kinds of solvent and the mixing ratios of solvent, sodium chloride and $H_2O$. Optimum conditions for the preparation of high purity SA were THF as an organic solvent, volume ratio of organic solvent/SA : 1 (organic solvent 10ml/10ml SA) and the amount of NaCl addition 2.5g/10ml SA. The extraction degree of Si and the removal efficiency of Na at that point were 86.2% and 99.95% respectively and the purity of SA was 99.96%.

  • PDF

Quality Characteristics of White Bread Made with Makgeolli Sourdough (막걸리 사워도우로 제조한 식빵의 품질특성)

  • Yoo, Byeong Seong;Yun, Chun Sik;Kim, Hyun Ah;Chang, Yun Hee
    • Korean journal of food and cookery science
    • /
    • v.33 no.1
    • /
    • pp.94-103
    • /
    • 2017
  • Purpose: This study developed sourdough bread according to consumers' preferencesby shortening the sourdough fermentation process time through addition of makgeolli to sourdough. Methods: Fermentation time was measured by adding makgeolli and water to wheat flour. Results: Fermentation time was shorter than that of starter made with only water and flour, with 5 hours of primary fermentation and 3 hours of secondary fermentation. The optimum mixing ratio was 400 g of flour, 192 mL of water, and 48 mL of makgeolli. An increase in makgeolli sourdough content decreased crude protein and moisture contents of white bread. Crude fat and ash contents increased, and volume of bread was decreased as sourdough increased. The height of bread prepared with makgeolli sourdough was lower than that of bread without makgeolli sourdough. According to the results of the texture measurement, hardness, gumminess, and chewiness of sourdough group were lower as the addition of sourdough increased. Addition of makgeolli sourdough to white bread resulted in decreased lightness, but increased yellowness of white bread. Preparation of white bread with addition of sourdough improved taste and flavor compared to bread prepared only with wheat flour. Conclusion: The highest sensory preference was observed for white bread prepared whit 5 g of sourdough.

Crack and Cutting Resistance Properties of Natural Rubber(NR) Compounds with Silica/Carbon Black Dual Phase Filler (Silica/Carbon Black이 충전된 NR 가황물의 내Crack 및 내Cutting 특성)

  • Son, Woo-Jung;Cho, Ur-Ryung;Kim, Won-Ho
    • Elastomers and Composites
    • /
    • v.37 no.2
    • /
    • pp.86-98
    • /
    • 2002
  • The application of silica/carbon black dual phase fillers to natural rubber(NR) compound was investigated. When the amounts of filler content were restricted to 60phr, the optimum ratio of dual phase fillers were 25phr/35phr of silica/carbon black. It was found that these new fillers give better overall performance in comparison with carbon black in tear strength, crack resistance, and cutting resistance. Also the thermal degradation resistance of NR vulcanizates which were filled with dual phase fillers was better than that of the carbon black. Dual phase fillers filled NR vulcanizates showed better viscoelastic properties, like tan${\delta}$, for the wet skid resistance and rolling resistance of motor vehicle tires.

The study on the properties of binary mixture(crystalline silica/AIN) filled EMC(Epoxy Molding Compounds) (결정성 실리카/질화 알루미늄 혼합충진에 따른 EMC의 물성 연구)

  • 김원호;홍용우;배종우;황영훈;김부웅
    • Journal of the Microelectronics and Packaging Society
    • /
    • v.6 no.4
    • /
    • pp.41-48
    • /
    • 1999
  • Silica is the most popular materials as a filler of EMC for microelectronic packaging. However, because of its low thermal conductivity, the use of silica is restricted to parts requiring high thermal dissipation. The superior fluidity of EMC can be achieved with a combination of filler size distribution. In this study, physical properties of EMC filled with the crystalline silica(13$\mu\textrm{m}$) which have high fluidity and low cost and the AlN(2 $\mu\textrm{m}$) which have high thermal conductivity and low coefficient of thermal expansion were evaluated by changing the AlN/silica ratios. As a result of the evaluation of physical properties of EMC, the optimum mixing ratio of AlN/crystalline silica was 0.3/0.7. In this condition, binary mixture(AlN/crystalline silica) filled EMC showed superior properties, i.e., in the thermal conductivity, CTE, dielectric constant, flexural strength, and thermal shock resistance without reduction of fluidity.

  • PDF

Tunable $CO_2$ Laser for FIR Laser Pumping (원적외선 레이저 펌핑용 가변파장 $CO_2$ 레이저)

  • 진윤식;정기형;이헌주
    • Korean Journal of Optics and Photonics
    • /
    • v.1 no.2
    • /
    • pp.155-161
    • /
    • 1990
  • A Continous wave tunable $CO_2$ laser which is the slow axial flow type was construccted for the optical pumping of methyl alcohol ($CH_3OH$) laser. The reflective grating of 100 grooveslmm and 10.6$\mu\textrm{m}$ blaze wavelength was used to select wavelength. We have investigated continous outpu: power characteristics, and then have compared tuned output power profile with the gain curve of $CO_2$ laser. The optimum condition for maxium power was found at the gas mixing ratio 1 : 3 : 10 for $CO_2: N_2$ and He. under this condition the flow rate, pressure of lasing gas and discharge current are 9.5 llmin. 14 torr, and 55 mA respectively. The maxium output power was 55 Wlm. Output power of single wavelength operation was measured on 60 individual rotation vibration transitions in the P and K branches of the 9.5$\mu\textrm{m}$ band and 10.4$\mu\textrm{m}$ band in $CO_2$. The output power profile obtained from each band is well consistent with the gain curve of $CO_2$ laser and maxium tuned output power was 20 watt.

  • PDF

The Evaluation on the Environmental Effect of Coal-Ash and Phosphogypsum as the Evapotranspiration Final Cover Material (증발산 원리를 이용한 매립장 최종 복토공법의 복토재로서 석탄재와 인산석고의 환경적 영향 평가)

  • Yu, Chan;Yang, Kee-Sok
    • Journal of the Korean GEO-environmental Society
    • /
    • v.6 no.1
    • /
    • pp.15-21
    • /
    • 2005
  • In this study, the utilization of coal-ash and phosphogypsum was considered as the evapotranspiration final landfill cover(ET cover) material. Cover material considered was the mixture of the weathered granite soil, coal-ash and phosphogypsum and so we sequentially performed the leaching test, column test and field model test to investigate the environmental effects of mixtures of coal-ash and phosphogypsum. In the leaching test, all materials had lower heavy metal concentration than the regulated threshold values. The column test and the review of related regulations were carried out to determine the optimum mixing ratio(OMR) and OMR was soil(4):coal-ash(1): phosphogypsum(1) on the volume base, which was applied to field model test. Field model tests were continued from February to June, 2004 in the soil box that was constructed with cement block. It was verified that coal-ash and phospogypsum mixed with soil was safe environmentally and the mixture of both wastes could improve the water retention capacity of cover materials.

  • PDF

A mixture of seaweed extracts and glycosaminoglycans from sea squirts inhibits α-MSH-induced melanogenesis in B16F10 melanoma cells

  • Wang, Lei;Cui, Yong Ri;Yang, Hye-Won;Lee, Hyo Geun;Ko, Ju-Young;Jeon, You-Jin
    • Fisheries and Aquatic Sciences
    • /
    • v.22 no.5
    • /
    • pp.11.1-11.8
    • /
    • 2019
  • Background: In the present study, the skin-whitening effects of a marine-sourced mixture that includes a fucoidanrich extract of Undaria pinnatifida (UPEF), a phlorotannin-rich extract of Ecklonia cava (ECE), and glycosaminoglycans (GAGs) from sea squirt skin were investigated. Methods: The whitening effects of the mixture and its components were evaluated by measuring the inhibition of mushroom tyrosinase and melanin synthesis in alpha-melanocyte-stimulating hormone (${\alpha}$-MSH)-stimulated B16F10 melanoma cells. Results: Each component alone markedly inhibited mushroom tyrosinase in a dose-dependent manner, and in ${\alpha}$-MSH-stimulated B16F10 cells, they inhibited melanin synthesis and were cytotoxic. However, the whitening effects of UPEF, ECE, and GAGs in combination were greater than those of each component alone. A mixture in the ratio of 4:5:1 (UEG-451) showed the strongest activity without cytotoxicity. Further study suggested that UEG-451 inhibits ${\alpha}$-MSH-stimulated melanogenesis in B16F10 cells by downregulating tyrosinase and tyrosinase-related proteins, such as TRP-1 and TRP-2, via the inhibition of MITF expression. Conclusions: These results suggest that mixing the different components at optimum ratios might be an effective way to improve their bioactivities and reduce toxicity and that UEG-451 possesses strong whitening effects that could be used in the cosmetic industry.

Synthesis of Organic-inorganic Core-shell Nanoparticle Powder using Immersion Annealing Process (담금 어닐링을 이용한 유·무기 코어-쉘 나노입자 파우더 합성법)

  • Choi, Young Joong;Jung, Hyunsung;Bang, Jiwon;Park, Woon Ik
    • Journal of the Microelectronics and Packaging Society
    • /
    • v.25 no.4
    • /
    • pp.35-40
    • /
    • 2018
  • Simple and useful synthetic process to control the morphology of block copolymers (BCPs) is required for implementation in various device applications. However, the conventional method to use colloidal templates is not enough to realize the production of pure and massive core-shell nanoparticles due to the cost-intensive complex process. Here, we introduce a novel and facile synthesis method to realize the formation of core-shell $SiO_x$ nanoparticle power by employing an immersion annealing of a sphere-forming poly(styrene-b-dimethylsiloxane) (PS-b-PDMS) BCP. We successfully obtained a PS-encapsulated $SiO_x$ nanoparticle with a diameter of ~20 nm. In addition, we analyzed how the mixing ratio of heptane/ethanol affects the BCP morphology of self-assembled PS-b-PDMS nanoparticles, showing a worm-like structure under the optimum immersion conditions. This useful approach is expected to be extendable to other solvent-based BCP synthesis, providing a new guideline for unique BCP production.