• Journal of Electrochemical Science and Technology
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• 제14권3호
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• pp.215-221
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• 2023

A rapid and environment-friendly electrochemical sensor to determine the chemical oxygen demand (COD) has been developed. The boron-doped diamond (BDD) thin-film electrode is employed as the anode, which fully oxidizes organic pollutants and provides a current response in proportion to the COD values of the sample solution. The BDD-based amperometric COD sensor is optimized in terms of the applied potential and the solution pH. At the optimized conditions, the COD sensor exhibits a linear range of 0 to 80 mg/L and the detection limit of 1.1 mg/L. Using a set of model organic compounds, the electrochemical COD sensor is compared with the conventional dichromate COD method. The result shows an excellent correlation between the two methods.

• Bulletin of the Korean Chemical Society
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• 제32권2호
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• pp.599-604
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• 2011

In typical proteomic analysis, trypsin digestion is one of the most time-consuming steps. Conventional proteomic sample preparation methods use an overnight trypsin digestion method. In this study, we compared high-pressure cycling technology (PCT) during enzyme digestion for proteome analysis to the conventional method. We examined the effect of PCT on enzyme activity at temperatures of 25, 37, and $50^{\circ}C$. Although a fast digestion (1 h) was used for the standard protein mixture analysis, the PCT-assisted method with urea showed better results for protein sequence coverage and the number of peptides identified compared with the conventional method. There was no significant difference between temperatures for PCT-assisted digestion; however, we selected PCT-assisted digestion with urea at $25^{\circ}C$ as an optimized method for fast enzyme digestion, based on peptide carbamylation at these conditions. The optimized method was used for stem cell proteome analysis. We identified 233, 264 and 137 proteins using the conventional method with urea at $37^{\circ}C$ for 16h, the PCT-assisted digestion with urea at $25^{\circ}C$ for 1 h, and the non-PCT-assisted digestion with urea at $25^{\circ}C$ for 1 h, respectively. A comparison of these results suggests that PCT enhanced the enzyme digestion by permitting better access to cleavage sites on the proteins.

• The Korean Journal of Physiology and Pharmacology
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• 제13권5호
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• pp.373-378
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• 2009

Cerebellar Purkinje cells (PCs) play a crucial role in motor functions and their progressive degeneration is closely associated with spinocerebellar ataxias. Although immunohistochemical (IHC) analysis can provide a valuable tool for understanding the pathophysiology of PC disorders, the method validation of IHC analysis with cerebellar tissue specimens is unclear. Here we present an optimized and validated IHC method using antibodies to calbindin D28k, a specific PC marker in the cerebellum. To achieve the desired sensitivity, specificity, and reproducibility, we modified IHC analysis procedures for cerebellar tissues. We found that the sensitivity of staining varies depending on the commercial source of primary antibody. In addition, we showed that a biotin-free signal amplification method using a horseradish peroxidase polymer-conjugated secondary antibody increases both the sensitivity and specificity of ICH analysis. Furthermore, we demonstrated that dye filtration using a $0.22\;{\mu}m$ filter eliminates or minimizes nonspecific staining while preserving the analytical sensitivity. These results suggest that our protocol can be adapted for future investigations aiming to understand the pathophysiology of cerebellar PC disorders and to evaluate the efficacy of therapeutic strategies for treating' these diseases.

• EDISON SW 활용 경진대회 논문집
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• 제5회(2016년)
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• pp.246-255
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• 2016

When spring of the suspension is exerted by an external load, a car should be designed to prevent predictable damages and designed for a ride comfort. We used experiments design to design VON-MISES STRESS and K, a constant, of spring of suspension which is installed in a car as a goal level. We analyzed the result from Edison's Elastic - Plastic Analysis SW(CSD_EPLAST) by setting D, d, n as external diameter of coil, internal diameter of coil, the number of total coil respectively. The experiment design let the outcome be as Full-second order by using Box-Behnken which is one of response surface methods. Experimented and analyzed results based on the established experiments design, We found out design parameter which has desired VON-MISES STRESS and the constant K. Additionally, we predicted life time of when the external load was exerted by repeated load by using fatigue equation, and verification of plastic deformation has also been made. Additionally we interpreted a model, which is formed by optimized design parameter, with linear analysis and non-linear analysis, at the same time we also analyzed plastic deformation with the values from the both models. Finally, we predicted fatigue life of optimized model by using fatigue estimation theory and also evaluated a ride comfort with oscillation analysis.

• 한국해양공학회지
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• 제23권2호
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• pp.104-109
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• 2009

In the view of the importance of material reduction due to the jump in oil and steel prices, an optimized structural system for lifting lugs was developed. Such a system is needed hundreds of thousands of times a year. A direct design process was added to this developed optimized system to increase the design efficiency and provide a way of directly inserting a designer's decisions into the design system process. In order to verify the system efficiency and convenience, several new prototype lug shapes were suggested using the developed system. From these research results, it was found that the slope of the main plate of the lug structure has a tendency to move from about 45 degrees to about 60 degrees and the design weight was reduced from an initial value of about 32kgf to about $15{\sim}19kg_f$ after the redesign. Based on these initial research results, an efficient reduction in steel weight was expected considering the enormous consumption of lug structures per year. Additionally, a more detail structural analysis through local strength evaluations will be performed to verify the efficiency of the optimum structural design for a lug structure.

• 한국정밀공학회지
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• 제23권1호
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• pp.145-151
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• 2006

Wheel bearing unit has been exclusively applied to car wheel supporting device. The seal for wheel bearing is required to have both high sealing effects and low reaction forces because wheel bearing are operated on adverse environmental conditions such as mud and splash water. High sealing effects are for the protection of bearing ball wear from dust influx. In order to ensure high sealing effects, it is a easiest way to increase contact force which are affected by geometric characteristics, material properties and interferences between seal and inner bearing but induces higher wear phenomena. Interferences in all variables are most important factor to determine the performance of wheel bearing. In this study, optimization of interference amount was performed with finite element analysis with commercial code ABAQUS. For the sake of finite element analysis, tensile tests of rubber material were conducted and governing equation of nonlinear behavior was achieved. Hock-up bearing was manufactured with optimized interference amount. Results of torque and mud spray tests using this bearing unit are performed. Less torque and moisture influx of bearing with optimized interference amount is evidence to validity of this study.

• 한국정밀공학회지
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• 제21권2호
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• pp.27-34
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• 2004

In the injection molding of plastic optical lenses, the molding conditions have critical effects on the quality of the molded lenses. Since there are many molding parameters involved in injection molding process, determination of the molding conditions for lens molding is very important in order to precisely control the surface contours of an optical lens. Therefore this paper presents the application of neural network in suggesting the optimized molding conditions for improving the quality of molded parts based on data of FE Analysis carried out through CAE software, Timon-3D. Suggested model in this paper, which serves to learn from the data of FE Analysis and induce the values for optimized molding conditions. has been implemented for searching the molding conditions without void and with minimized thickness shrinkage at lens center of injection molding optical lens. As the result of this study. we have confirmed that void creation at the inside of lens is primarily determined by mold temperature and thickness shrinkage at center of lens is primarily determined by the parameters such as holding pressure and mold temperature.

• 소성∙가공
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• 제20권3호
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• pp.214-221
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• 2011

In recent years, automotive seat components have been manufactured by the fine blanking process, allowing an improvement of dimensional accuracy at sheared surface in series production. However, the rollover has increased and die failures have occurred more frequently when manufacturing gears by fine blanking. Consequently, important goals for manufacturing seat recliner parts with gears have been to decrease the rollover as well as to improve the tool life. In this study, the half blanking and shaving processes were introduced to improve aforementioned problems for the lower tooth, the main component of a seat recliner. For this purpose, the half blanking process was optimized using the finite element (FE) analysis and design of experiment (DOE). The optimized conditions resulting from this study were an offset of 0.2 mm, a clearance of 0.1 mm and a penetration depth of 4.5 mm. Fine blanking experiment conducted under the optimal condition resulted in a rollover depth decrease from 1.9 to 1.3 mm, and no die failure occurrence.

• 한국전자통신학회논문지
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• 제14권6호
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• pp.1139-1144
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• 2019

정밀측정장비의 성능을 진단 및 확인하는 교정 시스템은 교정결과의 신뢰성 확보를 위해 국제규격의 요구조건을 준수함으로 교정결과의 오판정 위험을 최소화하고 있다. 본 논문에서는 교정기관에서 고성능의 장비를 획득 및 운영하기에 불가능한 경우 불확도 요인 분석을 통한 교정 시스템 적합성 평가 방안과 가드밴드 기법을 이용하여 성능기준을 대체하는 최적화된 시험기준 산출모형을 제안한다. 제안된 방법은 교정 시스템의 정량적인 평가기준과 국제규격에서 요구되는 허위수락위험 확률 기준을 충족을 위한 최적화된 시험기준을 제공한다.

• 한국생산제조학회지
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• 제26권4호
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• pp.410-417
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• 2017

Hydro-forming is being employed increasingly to realize lightweight vehicular parts. The bumper beam produced by this process weighs 30% less than the conventional products with equal stiffness. However, hydro-forming involves complex parameters to obtain the target geometry and low residual stress. Parametric studies are conducted using finite element analysis to obtain optimized process conditions. Through these numerical approaches, the internal and holding pressures and feeder forward stroke along the extruded direction are optimized to achieve low residual stress and to minimize springback. The numerical results are verified by experimental observations made by employing a three-dimensional laser scanner. The numerical and experimental results are compared in terms of the springback. Both results show similar tendencies.