• Microbiology and Biotechnology Letters
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• v.36 no.1
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• pp.28-33
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• 2008

The expression vector (pWHM3-TR1R2) for sprT gene encoding Streptomyces griseus trypsin (SGT) followed by two regulatory genes, sgtR1 and sgtR2, was introduced into Streptomyces lividans TK24 and Streptomyces griseus IFO 13350. Various media with different compositions were used to maximize the productivity of SGT in the recombinant trains. he SGT productivity was best when the transformant of S. lividans TK24 was cultivated in R2YE medium (0.74 unit/mL) at 5 days of cultivation. C5/L (0.66 unit/mL) medium also gave a good productivity, but Livid (0.08 unit/mL) and NDSK (0.06 unit/mL) yielded poor productivities. S. griseus IFO 13350/pWHM3-TR1R2 produced SGT by 1.518 unit/mL (C5/L), 1.284unit/mL (R2YE),0.932 unit/mL (NDSK), and 0.295 unit/mL (Livid) at 7 days of cultivation, which was much higher than those from S. lividans TK24/TR1R2. The SGT protein was purified from the culture broth of S. griseus IFO 13350/pWHM3-TR1R2 in C5/L to homogeneity via ammonium sulfate fractionation, and CM-sepharose and SP-sepharose column chromatographies. The specific activity of purified SGT was 69,252 unit/mg, and the final purification fold and recovery yield were 6.5 and 1.4%, respectively.

• Journal of Korean Society of Environmental Engineers
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• v.29 no.2
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• pp.229-234
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• 2007

In the present work, the sequencing batch reactor process of two-sludge type was optimized. The effects of solid retention time, hydraulic retention time, length of biosorption phase and temperature variation were investigated, respectively. In the T-N removal, the long solid retention time was favored. It was speculated that SCOD biosorption efficiency was higher in long solid retention time than in short solid retention time. In the comparison of hydraulic retention time, the removal efficiency of $NH_4^+-N$ and T-N were almost same in all applied hydraulic retention times which were 8 hr, 10 hr and 15 hr. It was observed that there was no need to have the hydraulic retention time more than 20 min in biosorption phase for enhancement of T-N removal efficiency. An experimental comparison of removal efficiencies with different temperature conditions was carried out. Decrease of temperature didn't affect the performance of the process, however, phosphorus removal efficiency was a little higher at low temperature than high temperature. Consequently, the process developed in this study was much amenable to wastewater treatment which was conducted in the low temperature and high loading rate.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.6
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• pp.621-627
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• 2008

In this study, a technique of speciation and determination of the trace inorganic arsenic(As(III) and As(V)) in water sample using HPLC-DRC-ICP-MS has been developed. Isocratic mobile phase of 10 mM ammonium nitrate and 10 mM ammonium phosphate monobasic was used and methanol(5 v/v%) was used as flushing solvent. Selection of the best flow rate of reaction gas, O$_2$, and optimization of the parameters such as pH and flow rate of mobile phase, and injection volume of sample for the separation and detection of arsenic species were carried out. The oxygen flow rate of 0.5 mL/min, pH of 9.4 and flow rate of 1.5 mL/min of mobile phase, and injection volume of sample of 100 $\mu$L were found to be the best parameters for the speciation and determination of arsenic species. The analytical features of the method were detection limit 0.10 and 0.08 $\mu$g/L, precision(RSD) 4.3% and 3.6%, and recovery 95.2% and 96.4% for As(III) and As(V), respectively. Analysis time was 4 minutes per sample. Linear calibration graphs with r$^2$ = 0.998 were obtained for both As(III) and As(V). Speciation analysis of arsenic species in the raw water samples collected from the tributary streams to Han River and main stream of Paldnag were performed by the proposed method. The concentrations of As(III) ranged from 0.10 to 0.22 $\mu$g/L and As(V) concentrations ranged from 0.44 to 1.19 $\mu$g/L, and 93.5% of total arsenic was found to be As(V).

• Environmental and Resource Economics Review
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• v.15 no.4
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• pp.693-712
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• 2006

A pre-feasibility test is done for renewable energy hybrid power systems at off~grid islands in which the current power supply is provided only by diesel generation. We apply Homer (Hybrid Optimization Model for Electric Renewables) which was developed by the National Renewable Energy Laboratory (NREL) for the analysis to identify the cost-minimizing combination of power generating facilities for the given load profiles. Chuja-Do, Geomun-Do and Youngsan-Do have been selected for our analysis considering the wind resources data of the Korea Institute of Energy Research (KIER). Information on wind speed, solar radiation and temperature is also used for the analysis. System component cost information from overseas market has been used due to the lack of domestic information. Site specific Load profile for electricity demand for those islands are reconstructed based on the partial survey results obtained form other sources. The LCOE of the least cost hybrid power systems for Chuja-Do, Geomun-Do and Youngsan-Do are $0.278/kWh, $0.234/kWh and $0.353/kWh, respectively Considering the fact that diesel generation is being subsidized at the price of $0.300/kWh by the government, first 2 cases are economically feasible for the introduction of renewable energy hybrid systems to those islands. But the third case of Youngsan-Do does not meet the criteria. The basic differences of these pre-feasibility test results are from the differences of the site specific renewable energy conditions, especially wind resources. In summary, promoting hybrid systems in the off-grid remote island should be based on the economic feasibility test results. Not all the off-grid islands are feasible for introducing this renewable energy hybrid system.

• Applied Biological Chemistry
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• v.41 no.6
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• pp.465-470
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• 1998

For the need of bio-degradable flocculant in stage of wastewater treatment, some cultural conditions of bioflocculant production were optimized with Aeromonas hydrophila KH-54. About 260 strains of type culture and bacteria isolated from marsh, pond, activated sludge, etc were examined for their ability to flocculate kaolin particles and swine wastewater. Among them, KH-54 showed the highest flocculating activity and was identified as Aeromonas hydrophila according to the cultural, morphological and physiological properties. The maximum production of the flocculant secreted by Aeromonas hydrophila KH-54 was observed in culture medium containing 2.0% mannitol, 0.05% ammonium chloride, 0.02% potassium phosphate dibasic, 0.01% $MgSO_4{\cdot}7H_2O$ and 0.05% yeast extract at initial pH 7.0 when cultured on rotary shaker controlled at $25^{\circ}C$ and 150 rpm. Under the optimized condition, the flocculating ability reached to 770 units/ml of kaolin flocculating activity and 81% of NTU removal efficiency against swine wastewater after 4 days cultivation. The bioflocculant was also effective on various organic wastewaters other than swine wastewater, showing NTU removal rate ranging from 92% to 34%.

• Current Research on Agriculture and Life Sciences
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• v.6
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• pp.163-169
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• 1988

This study was conducted to predict the density changes according to concentration and temperature changes under freezing point. This information is needed for the design of freezing equipment and for the efficient utilization of refrigerating system. Orange juice, Apple juice, Grape juice and Sucrose solution were used for the measurement of density in this study at the temperature range from $-5^{\circ}C$ to $-40^{\circ}C$ and at the concentration range from 10 to 40%. The unfrozen water fraction of samples was determined by Heldman's method. The density values were determined by measuring the weight of a frozen solution at each temperature with a known volume. Solutions were placed in the thick-walled aluminum tubes. When the solution was frozen the excess ice was removed with a razor until the surface of the ice was flush with the top of the aluminum tube. The tube and ice were weighted immediately. Knowing the volume, tare weight, and final weight, the density could be determined. With this procedure, the data of density and unfrozen water fraction for fruit juices and sucrose solution were collected. The density prediction models of fruit juices and sucrose solution under freezing point were established by the optimization computer program with measured experimental data.

• Journal of the Korean Applied Science and Technology
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• v.34 no.3
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• pp.451-460
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• 2017

This study optimized the extraction of antioxidants from Gugija (Lycium chinensis Mill). To determine operational parameters, including ethanol concentration ($X_1$, 0~80%) and extraction time ($X_2$, 1~5 hr), response surface methodology was applied to monitor yield, anthocyanins, flavonoids and DPPH radical scavenging activity. Coefficients of determinations ($R^2$) of the models were range of 0.8645~0.9859 (p<0.01~0.1) in dependant parameters. Yield of Gugija extracts was maximized 23.12% in extraction conditions of 4.22 h at 8.25% ethanol. Anthocyanins was maximized 1.43 (OD in 530 nm) in extraction conditions of 3.06 h at 79.98% ethanol. Flavonoids was maximized $3,100{\mu}g/100g$ in extraction conditions of 3.37 h at 67.02% ethanol. DPPH radical scavenging activity was maximized 96.93% in extraction conditions of 1.67 h at 69.81% ethanol. Optimum extraction conditions (2.5 h extraction at 70% ethanol) were obtained by superimposing the contour maps with regard to anthocyanins, flavonoids and DPPH radical scavenging activity of Gugija. Maximum values of anthocyanins, flavonoids and DPPH radical scavenging activity in optimum extraction condition were 1.0080 (OD in 530 nm), $3,145{\mu}g/100g$, 96.96%, respectively. But values of anthocyanins, flavonoids and DPPH radical scavenging activity in water extraction condition (1 h at water) were 0.4652 (OD in 530 nm), $1,633{\mu}g/100g$, 86.98%, respectively.

• The Journal of the Korea Contents Association
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• v.9 no.8
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• pp.248-254
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• 2009

The aim of this study is to optimize the light source combination which can maximize the capability of the illuminance and color temperature of circadian lighting apparatus. To achieve this goal, the circadian lighting apparatus was consisted of two different types of fluorescent lamps having different color temperature of 2000K and 8000K, respectively, and the capability of the illuminance and color temperature of circadian lighting apparatus was evaluated by optical simulation as the number of the respective lamps were varied. Considering the Kruithof's curve and exceptional cases, the ranges of illuminance and color temperature for the living activities were reclassified in 4 groups - gathering, studying, relaxing and sleeping - so that the target range of illuminance and color temperature of lighting apparatus was settled. As a result, in the case of adopting two fixtures in which four 2000K lamps and five 8000K lamps were consisted, respectively to one fixture, the highest illuminance was expected at 4000K and over 500lx of illuminance was calculated between 3000K and 6000K. Through the optimized combination of light sources, the range of illuminance and color temperature were calculated as $44{\sim}750lx$ and $2500{\sim}6500K$, respectively.

• The Journal of the Korea Contents Association
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• v.17 no.11
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• pp.383-388
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• 2017

The purpose of this study was to compare radiation dose among workers in the radiation zone and to compare the doses of students in clinical practice in the same area to provide basic data on optimization of radiation protection. The subjects were 121 radiation related workers, 36 radiation workers, and 121 students who completed 8 weeks of clinical practice from Jan. 2016 to Dec. The depth and surface dose between the radiation related workers and the radiation workers were the highest with $.7440{\pm}1.676mSv$ and $.7753{\pm}1.730mSv$, respectively, and statistically significant (p<.01). Among the three groups, the depth dose was the highest at $.143{\pm}.136mSv$ for clinical practice students and the highest at surface dose of $.1513{\pm}.139mSv$. The lowest in both cases, The mean difference between the two groups was statistically significant (p<.01). In conclusion, it is necessary to manage thoroughly according to the ALARA(As Low As Reasonably Achievable) principle. Especially, it is necessary to systematically manage the dose of radiation for clinical students who are in the blind spot of radiation safety management.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.2
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• pp.695-701
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• 2018

Research into exhaust heat recovery has been actively carried out to improve the thermal efficiency of internal combustion engines. In this study, the performance of thermoelectric generation from exhaust heat recovery for motorcycle engines was analyzed by 1-D thermo-fluid simulation. GT-SUITE, which was developed by Gamma Tech., was used for the simulation of the internal combustion engine and thermoelectric generation system. The basic performance of the engine was analyzed in the range of engine speed of 1000~7000 rpm and engine load of 0~100%. The ratio of exhaust heat energy to fuel chemical energy was found to be about 40~60%. A combined simulation of the engine model and thermoelectric generation model was carried out to analyze the voltage, current and power generated by the thermoelectric material. The generation characteristics of the thermoelectric material was dominantly affected by the exhaust gas temperature. The maximum generated power of the current thermoelectric generation system was found to be about 2.2% of the total exhaust heat energy. The design optimization of the thermoelectric generation system will be carried out to maximize its power generation and economic feasibility.