• Journal of Microbiology
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• v.45 no.2
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• pp.158-167
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• 2007

In this study, we have cloned a novel cDNA encoding for a papain-family cysteine protease from the Uni-ZAP XR cDNA library of the polychaete, Periserrula leucophryna. This gene was expressed in Escherichia coli using the T7 promoter system, and the protease was characterized after partial purification. First, the partial DNA fragment (498 bp) was amplified from the total RNA via RT-PCR using degenerated primers derived from the conserved region of cysteine protease. The full-length cDNA of cysteine protease (PLCP) was prepared via the screening of the Uni-ZAP XR cDNA library using the $^{32}P-labeled$ partial DNA fragment. As a result, the PLCP gene was determined to consist of a 2591 bp nucleotide sequence (CDS: 173-1024 bp) which encodes for a 283-amino acid polypeptide, which is itself composed of an 59-residue signal sequence, a 6-residue propeptide, a 218-residue mature protein, and a long 3'-noncoding region encompassing 1564 bp. The predicted molecular weights of the preproprotein and the mature protein were calculated as 31.8 kDa and 25 kDa, respectively. The results of sequence analysis and alignment revealed a significant degree of sequence similarity with other eukaryotic cysteine proteases, including the conserved catalytic triad of the $Cys^{90},\;His^{226},\;and\;Asn^{250}$ residues which characterize the C1 family of papain-like cysteine protease. The nucleotide and amino acid sequences of the novel gene were deposited into the GenBank database under the accession numbers, AY390282 and AAR27011, respectively. The results of Northern blot analysis revealed the 2.5 kb size of the transcript and ubiquitous expression throughout the entirety of the body, head, gut, and skin, which suggested that the PLCP may be grouped within the cathepsin F-like proteases. The region encoding for the mature form of the protease was then subcloned into the pT7-7 expression vector following PCR amplification using the designed primers, including the initiation and termination codons. The recombinant cysteine proteases were generated in a range of 6.3 % to 12.5 % of the total cell proteins in the E. coli BL21(DE3) strain for 8 transformants. The results of SDS-PAGE and Western blot analysis indicated that a cysteine protease of approximately 25 kDa (mature form) was generated. The optimal pH and temperature of the enzyme were determined to be approximately 9.5 and $35^{\circ}C$, respectively, thereby indicating that the cysteine protease is a member of the alkaline protease group. The evaluation of substrate specificity indicated that the purified protease was more active towards Arg-X or Lys-X and did not efficiently cleave the substrates with non-polar amino acids at the P1 site. The PLCP evidenced fibrinolytic activity on the plasminogen-free fibrin plate test.

• Food Science of Animal Resources
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• v.37 no.5
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• pp.663-669
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• 2017

This study were estimated the contribution of carcass traits to unit price, to analyze the marbling score as a categorical variable rather than a numerical variable, and to develop an optimal model that also includes the holiday effect and the raising period. The data for this study were acquired from the Quality Evaluation of the Korea Institute for Animal Products, and consisted of the trading records of 1,613,699 heads at 12 wholesale markets from 2010 to 2014. The unit price of a cow was estimated from the following parameters: -52.50 Won/mm, $8.93Won/cm^2$, 7.20 Won/kg, and -1.04 Won/day for backfat thickness, eye muscle area, carcass weight, and raising period, respectively. Parameters for the dummy variables of marbling scores varied from 0 to 8328.74 Won/kg, which means that each marbling score grade had a different price value. The unit price of a steer was estimated from the following parameters: - 92.12 Won/mm, $20.22Won/cm^2$, 1.30 Won/kg, and -1.72 Won/day for backfat thickness, eye muscle area, carcass weights, and raising period, respectively. Parameters for dummy variables of marbling scores varied from 0 to 7338.80 Won/kg, which means that the grades of each marbling score had different price values. The unit price of sales during traditional holidays was significantly higher (827.71 Won/kg for cows, and 645.15 Won/kg for steers) than during non-holidays.We conclude that the use of categorical values for marbling scores would be needed to evaluate the price of Hanwoo beef using multiple regression analysis based on carcass traits and environmental factors.

• KSBB Journal
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• v.14 no.5
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• pp.578-584
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• 1999

The lectin was purified through 0.15 M NaCl extraction, ammonium sulfate precipitation, sepharose 4B affinity chromatography and gel filtration using sephadex G-150 from the leaves of Visum album collected in Mt. Duk Yu. The final gel filtration step resulted in 11.64 folds purification with 0.14% of recovery yield. We also performed biochemical characterization of the purified Visum album lectin. HPLC analysis of lectin purified by gel filtration revealed a singel peak. The analysis of the purified lectin by SDS-PAGE showed a tetramer composed of two identical subunits with molecular weights of 32 and 30 kDa. The lectin was a glycoprotein containing 14.4% carbohydrate, which consist of glucose, fructose, arabinose and xylose, and the amino acids such as phenylalanine, lysine and tyrosine. The purified lectin agglutinated human red blood cell types with similar potency, but when tested against red blood cells from mouse, bovine, rabbit, chicken and porcine, significant difference in potency were observed. Hemaggluting activity was inhibited by D-galactose, D-mannose, D-lactose and D-raffinose, but not by D-glucose, D-glucosamine, D-mannosamine, L-fructose, D-xylose, D-arabinose, D-galacturonic acid, D-fructose, L-rhamnose and N-acetyl-D-galactosamine. The optimal pH and thermal stability of the purified lectin were pH 4.0-7.0 and 20-5$0^{\circ}C$, respectively.

• The Journal of Internal Korean Medicine
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• v.30 no.2
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• pp.270-287
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• 2009

Objective : Yinjinchunggan-tang(YJCGT) is reported previously as having theraputic effects on hepatitis such as anti-implammatory, anti-apoptotic, anti-viral(HBV), etc. Though this prescription is not studied on its anti-fibrogenic effect, it is still expected to have the effect in the liver. Thus, this study was performed to investigate the anti-fibrogenic effect of YJCGT on thioacetamide(TAA)-induced liver fibrosis in rats. Method: Rat liver fibrosis was induced by intraperitoneal TAA injection(150mg/kg) 3 times a week for 5 weeks. After the YJCGT (YJCGT 1g/kg, YJCGT 2g/kg)-treatment, body weight, liver and spleen weights, liver function test, the complete blood count and the portal pressure were studied. In addition, gene expressions of ASMA, procollagen type Ia2, MMP2, TIMP1 and TIMP2, all of which are known to be associated with liver fibrosis, were analyzed by RT-PCR. After YJCGT (YJCGT 1g/kg, YJCGT 2g/kg) treatment, percentages of collagen in TAA-induced rat liver tissue were measured by image analyzer. Results : The body weight of the normal group increased more than that of the control and YJCGT-treated groups. The AST level of the YJCGT lg/kg-treated group significantly decreased compared to that of the control. The ALT and the GGT levels of the YJCGT 2g/kg-treated group significantly increased compared to those of the control. In the YJCGT-treated groups. WBC, RBC and Hgb elevated by TAA injection decreased but platelet count increased. In the YJCGT lg/kg-treated group, the portal pressure elevated by TAA injection significantly decreased. The significant decreases in the gene expressions of procollagen type Ia2, MMP2 and TIMP2 were observed in the YJCGT-treated groups. In histological findings. TAA injections caused severe liver fibrosis, but the YJCGT treatment significantly reduced the amounts of hepatic collagens. Conclusions: These results suggest that YJCGT has beneficial effects on the treatment of patients with liver cirrhosis as well as chronic hepatitis. Further study should be done to decide the optimal concentration of the YJCGT for the treatment of liver cirrhosis.

• Microbiology and Biotechnology Letters
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• v.32 no.3
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• pp.230-237
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• 2004

In this study nitroreductase from Pseudomonas sp. HK-6 capable of degrading 2,4,6-trinitrotoluene (TNT) was characterized. Through a series of purification process including ammonium sulfate precipitation, DEAE-sepharose, and Q-sepharose, three different fractions I, II, and III having the enzyme activity of NTRs whose molecular weights were approximately 27 kDa were detected in fractions from HK-6 cells. Specific activity of the three fractions were approximately 4.85 unit/mg, 5.47 unit/mg, and 5.01 unit/mg, and concentrated to 9.0-, 10.1-, and 9.3-fold compared to crude extract, respectively. The optimal pH and temperature for the three NTR fractions were approximately 7.5 and $30^{\circ}C$, respectively. Metal ions, $Ag^{＋}$ , $Cu^{ 2+}$, $Hg^{2＋}$ inhibited approximately 70% of enzymes activities of all NTR, while $Fe^{2＋}$ did not stimulate or inhibit the activities. Monitoring the effect of chemicals on the enzyme activity revealed that those NTR fractions lost enzyme activity in presence of $\beta$-mercaptoethanol, but were a little influenced by dithiothreitol, EDTA and NaCl. The three NTR fractions demonstrated enzyme activities for nitrobenzene and RDX as well as TNT.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.8
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• pp.1034-1042
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• 2010

This study aimed to determine the optimum dietary crude protein level in a typical diet for fattening pigs fed ad libitum under normal climate conditions in Central Vietnam. One hundred and ninety two gilts of Mong Cai local breed (MC), $F_1$ Large White${\times}$Mong Cai and $F_2$ crossbreds of (Landrace${\times}$Mong Cai)${\times}$Large White were used. At the start of the experiment, Mong Cai pigs weighed 12 kg at 11 weeks of age, $F_1$ pigs 12.1 kg at 8 weeks of age and $F_2$ pigs 12.2 kg at 8 weeks of age. Four diets differing in crude protein (CP) content (10.1, 13.1, 16.1 and 18.9% in DM) were formulated from rice bran, corn meal, cassava meal and fish meal. Calculated digestible energy content of the diets ranged from 13.5 to 13.8 MJ per kg DM. Pigs were housed individually in pens of $2.5\;m^2$ each and had ad libitum access to feed in a trough as well as water in bowls. The final weights after a growing period of 150 days were 66, 86 and 96 kg for MC, $F_1$ and $F_2$, respectively. Feed intake of MC pigs was highest at 13.1% CP while $F_1$ and $F_2$ had the highest feed intake at 16.1% CP. The results showed that for MC the maximum gain was obtained at levels between 13 to 16% CP. For the $F_1$ the maximum gain was at dietary protein levels of 16-17%. For $F_2$ the max gain was obtained at CP levels of 16 to 18%. Feed conversion was highest in MC pigs (~4.0) followed by $F_1$ (~3.3) and $F_2$ (~3.1), and within genotypes was lowest at the optimum CP level (p<0.05). Back fat thickness in MC (33.1 mm), $F_1$ (23.0 mm) and $F_2$ (20.5 mm) pigs was different and within genotypes was the lowest at intermediate CP levels. In conclusion, increasing the dietary crude protein contents in practical diets for pigs in Vietnam can increase production on small holder farms. Optimal performance for MC, $F_1$ and $F_2$ pigs is achieved at different dietary crude protein contents.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.42 no.1
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• pp.9-26
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• 2005

In the pursuit of ever higher levels of performance, recent computer systems have made use of deep pipeline, dynamic scheduling and multi-issue superscalar processor technologies. In this situations, branch prediction schemes are an essential part of modem microarchitectures because the penalty for a branch misprediction increases as pipelines deepen and the number of instructions issued per cycle increases. In this paper, we propose a novel branch prediction scheme, direction-gshare(d-gshare), to improve the prediction accuracy. At first, we model a neural network with the components that possibly affect the branch prediction accuracy, and analyze the variation of their weights based on the neural network information. Then, we newly add the component that has a high weight value to an original gshare scheme. We simulate our branch prediction scheme using Simple Scalar, a powerful event-driven simulator, and analyze the simulation results. Our results show that, compared to bimodal, two-level adaptive and gshare predictor, direction-gshare predictor(d-gshare. 3) outperforms, without additional hardware costs, by up to 4.1% and 1.5% in average for the default mont of embedded direction, and 11.8% in maximum and 3.7% in average for the optimal one.

• Journal of the Korean Association of Geographic Information Studies
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• v.20 no.1
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• pp.1-14
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• 2017

A land cover map provides basic information to help understand the current state of a region, but its utilization in the ecological research field has deteriorated due to limited temporal and spatial resolutions. The purpose of this study was to investigate the possibility of using a land cover map with data based on high resolution images acquired by UAV. Using the UAV, 10.5 cm orthoimages were obtained from the $2.5km^2$ study area, and land cover maps were obtained from object-based and pixel-based classification for comparison and analysis. From accuracy verification, classification accuracy was shown to be high, with a Kappa of 0.77 for the pixel-based classification and a Kappa of 0.82 for the object-based classification. The overall area ratios were similar, and good classification results were found in grasslands and wetlands. The optimal image segmentation weights for object-based classification were Scale=150, Shape=0.5, Compactness=0.5, and Color=1. Scale was the most influential factor in the weight selection process. Compared with the pixel-based classification, the object-based classification provides results that are easy to read because there is a clear boundary between objects. Compared with the land cover map from the Ministry of Environment (subdivision), it was effective for natural areas (forests, grasslands, wetlands, etc.) but not developed areas (roads, buildings, etc.). The application of an object-based classification method for land cover using UAV images can contribute to the field of ecological research with its advantages of rapidly updated data, good accuracy, and economical efficiency.

• Journal of the Economic Geographical Society of Korea
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• v.15 no.3
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• pp.437-455
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• 2012

Korea has recently constructed a number of renewable photovoltaic power plants in Jeolla province as an effort to provide environment-friendly energy. However, several problems appeared in the power plant locations because they were not appropriately chosen ignoring social-environmental perspectives. Consequently, locations of both currently existing photovoltaic power plants require an social and environmental evaluations. This study aims to provide appropriate photovoltaic power plants locations and evaluation of current photovoltaic power plants in Jeolla province. By presenting location analysis of photovoltaic power plants, this study would minimize environmental and social side effects regarding photovoltaic power plants. Kriging and Analytic Network Process (ANP) are applied as methodology. ANP generates correct weights in combining spatial data, so that the result would present optimal locations. In addition environmentally sensitive regions were excluded in the analysis process. The results show that South and West coastal areas have a number of appropriate locations for photovoltaic power plants. In addition, evaluating currently running photovoltaic power plant locations, total 23 out 81 are turned out to be inappropriately located. This study is expected to contribute avoiding social and environmental conflicts in photovoltaic power plant locations and present criteria in evaluating photovoltaic power plants.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.8 no.1
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• pp.1-23
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• 1998

Work-related Low Back Pain(LBP) is one of tile most important Issues in the field of industrial safety and health. Particularly, manual lifting is known as a major cause of work-related LBP and impairment. Total number of 163 manual lifting tasks in motor assembly processes were investigated. The 1981 and the 1994 equations developed by National Institute for Occupational Safety and Health(NIOSH) were applied to evaluate potential hazards of lifting-related LBP. Comparisons between the 19R I and 1994 NIOSH criteria were made. The relationships between the NIOSH criteria and lifting-related LBP were also analyzed. The results of this study are as follows: 1. The values of Action Limit(AL) by the NIOSH 1981 lifting equation. Recommended Weight Limit(RWL) by the 1994 equation and the weight of the load handled at each manual lifting task were shown log-normal distributions. 2. LI'(the weight of tile load/AL) and LI(the weight of the load/RWL) were calculated estimate the physical stress imposed by each individual lifting task. As a result. 76.7% of the total LI' value exceeded 1 and 12.9% exceeded 3, and 84.7% of the total LI values exceeded 1 and 20.9% exceeded 3. 3. Bus 2 Department showed the highest rate of LI'>1 and LI>1 and Bus 1 Department showed the highest rate of LI'>3 and LI>3 4. In general, the RWLs by the 1994 equation were found lower than the ALs by the 1981 equation. It is assumed to he resulted from the fact that the 1994 equation includes methods evaluating asymmetrical lifting tasks and lifts of objects with less then optimal hand-container couplings, and also covers a larger range of work durations and lifting frequencies than the 1981 equation. 5. Significant correlations were found between LI' and incidence of LBP (R=0.734, p<0.05). LI and incidence of LBP(R=0.671. p<0.10) and load-weights and incidence of LBP(R=0.797, p<0.05). 6. Control measures are required to achieve the value of LI less than 1 for some tasks having high LI values. Engineering control is highly recommended for some tasks having the value of LI above 3.