• Journal of Microbiology
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• 제42권2호
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• pp.99-102
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• 2004

Bacteriophage PM2 has a closed circular form of double stranded DNA as a genome. This DNA from the phage is a useful source for nick-circle endonuclease assay in the fmol range. Due to difficulties in the maintenance of viral infectivity, storage conditions of the phage should be considered for the puri-fication of PM2 DNA. The proper condition for a short-term storage of less than 2 months is to keep the PM2 phage at 4$^{\circ}C$; whereas the proper condition for a long-term storage of the PM2 phage for over 2 months is to keep it under liquid nitrogen in 7.5 ％ glycerol. The optimal conditions for a high yield of phage progeny were also considered with the goal to achieve a successful PM2 DNA preparation. A MOI(Multiplicity Of Infection) of 0.03, in which the OD$\sub$600/ of the host bacteria was between 0.3 and 0.5, turned out to be optimal for the mass production of PM2 phage with a burst size of about 214. Considerations of PM2 genome size, and the concentrations and radiospecific activities of purified PM2 DNA, are required to measure the endonuclease activity in the fmol range. This study reports the proper quantitation of radioactivity and the yield of purified DNA based on these conditions.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권6호
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• pp.471-476
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• 2006

Pseudomonas aeruginosa F722 produces a biosurfactant (BS) during its degradation of carbon and hydrocarbon compounds. The culture conditions for upgrading the biosurfactant productivity were investigated. The concentration of the biosurfactant produced by P. aeruginosa F722 was 0.78 g/L in C-medium; however, this increased to 1.66 g/L in BS medium, which was experimentally adjusted to optimal conditions. $NaNO_{2}$ was found to be most effective for microbial growth, with an $O.D_{600nm}$ of 1.18 for 0.1 % $NaNO_{2}$. Microbial growths, according to the $O.D_{600nm}$ were 2.53, 2.68, 2.89, and 2.87 for glucose, glycerol, $n-C_{10},\;and\;n-C_{22}$, respectively. Clear zone diameters (cm), indicating biosurfactant activity, were 9.0, 8.8, 5.7, and 8.5 for glucose, glycerol, $n-C_{10},\;and\;n-C_{22}$, respectively. Microbial growth was not consistent with the biosurfactant activity. The best biosurfactant activity was found with a C/N ratio of 20. Under optimal culture condition, the average surface tension decreased from 70 to 30 mN/m after 5 days. With aeration of 1.0 vvm, the biosurfactant produced increased to 1.94 g/L (up to 20%) compared to that of 1.66 g/L with no aeration. With aeration, the velocities of glucose degradation during both the log and stationary growth phases increased from 0.25 and $0.18\;h^{-1}$ to 0.33 and $0.29\;h^{-1}$, respectively, and the time for the culture to arrive at the maximum clear zone diameter became shorter, from 80 down to 60 h with no aeration.

• 대한금속재료학회지
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• 제47권4호
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• pp.223-227
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• 2009

The precipitates, hardness, and tensile properties of Al-6.2Si-2.9Cu AC2B alloy were investigated with respect to solution treatment time at $500^{\circ}C$. $Al(Cu)-Al_2Cu$ eutectic, Si, ${\theta}-(Al_2Cu)$, and $Q-(Al_5Cu_2Mg_8Si_6)$ phases were observed in the as-cast specimen. With increasing the solution treatment time at $500^{\circ}C$, the $Al(Cu)-Al_2Cu$ eutectic and ${\theta}-(Al_2Cu)$ phases were gradually reduced and finally almost disappeared in 5 h. The mechanical properties, such as hardness, tensile strength, and elongation, were improved with solution treatment time until about 5 h due to the dissolution of the $Al_2Cu$ particles. With further holding time, the mechanical properties did not change much. The solution treated specimens for over 5 h at $500^{\circ}C$ exhibit almost the same tensile properties even after aging at $250^{\circ}C$ for 3.5 h. Accordingly, the optimal solution treatment condition of the Al-Si-Cu AC2B alloy is considered to be 5 h at $500^{\circ}C$.

• 한국기계가공학회지
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• 제12권3호
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• pp.7-12
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• 2013

In Die casting process, the problem of die degradation is often issued. In oder to increase of die life the material degradation of die steel was investigated using test core pins. Three test core pins were positioned in front of the gate entry and observed washout and soldering resistance during Mg die casting process. The test parameters are set as different commercial die materials, coatings condition and hardness of die surface. Usign 220t magnesium die casting machine was employed to cast AZ91 magnesium alloys. After 150 shots, macroscopic observation of die surface was carried out. Additional 50 cycles later, test pins were chemically cleaned with 5% HCl aqueous solution to find out the existence of washout and soldering layers. Microstructural characterization of die surface and the die roughness measurement were performed together. Computational simulation using AnyCasting program was also beneficial to correlate the extent of die damage with the position of test pin inside die cavity. As results, the optimal combination of die steel with productive coating as well as its hardness was drawn out. it will be helpful to decide the material and condition considering increasing of tool life.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2002년도 추계학술대회논문집
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• pp.363-368
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• 2002

A practical implementation method of squeeze-film aeroelastic disk vibration damping and its practical design performance are presented to provide a solution method to meet the tight TMR(Track Mis-Registration) design budget of high-TPI HDDs. Most previous research results are mainly based on the component-level study in the 'open-cover state' which is far from the realistic operation HDD condition. In this study, the squeeze-film disk damping effect is widely investigated under the realistic drive-level condition of 'enclosed-cover state.' It is found that the proper aeroelastic gap(s) between disk(s) and adjacent surface(s) to give significant vibration reduction in the enclosed HDD operating conditions can be achieved not only by classical well-known squeeze-film damping gaps such as very small 0.0x-millimeter level gaps which are not practically implementable in mass-production HDDs, but also by a few 0.x millimeter which is possible for designing realistic HDD design. The various experimental results including drive-level PES are also presented to prove feasibility of the optimal disk damper design for 93kTPI HDDs.

• 한국기계가공학회지
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• 제8권1호
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• pp.10-17
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• 2009

Spindle design is very important for the improvement of the competitive power in production cost of high quality machine tools. The important factor in spindle design is not only to improve the natural frequency of spindle but also to reduce displacement of spindle end. In this paper, parameters those influence on static and dynamic stiffness of high-speed spindle have selected form preceding studies. And those selected parameters are applied to Taguchi Method. To perform FEM analysis, bearing conditions are selected with optimized condition. To know how to improve static and dynamic stiffness of machine tool spindle, natural frequency and displacement of spindle end are obtained by FEM analysis. The Taguchi Method was used to draw optimized condition of bearing position and it's stiffness. From these results, amplitude of vibration is enough good less than $3{\mu}m$ pk-pk of the spindle of 40,000rpm manufactured in this work by the optimal design.

• 소성∙가공
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• 제14권3호
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• pp.245-250
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• 2005

Recently, micro machining technology for high precision mold becomes more interested for mass production of high performance optical parts micro-grooved on the surface, which is under very active development due to its effectiveness in the view point of optical performance. Mechanical micro machining technology now has more competitiveness on lithography, MEMS or LIGA processes which have some problems to fabricate especially cylinder type of groove in such as lenticular lens for illumination angle modulation system. In this study. a lenticular lens mold with U-type micro groove is fabricated making utilizing of the benefit of the mechanical micro machining technology. A shaping machining process is adapted using 3 axis degree of freedom micro machining system and single crystal natural diamond tool. A brass and a electroless nickel materials are used for mold fabrication. Machining force, chip shape and machined surface are investigated from the experiment and an optimal machining condition is found based on the examined problems from the micro cutting process.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2004년도 추계학술대회논문집
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• pp.54-57
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• 2004

Almost all injection molds have multi-cavity runner for mass production, which are designed with geometrically balanced runner system in order to minimize filling imbalance between cavity to cavity during processing. However, even though geometrically balanced runner is used, filling imbalances have sometimes been observed. These filling imbalances have historically been considered as result of uneven mold temperature and mold deflection, but it actually results from non-symmetrically shear, pressure, temperature distribution within melt material as it flows through the runner system. Filling imbalance could be decreased by modifying processing conditions that are related to shear, pressure, temperature such as injection rate, mold temperature, injection pressure, melt temperature. In this study, a series of experiment was conducted using Taguchi method to determine which processing condition influence as the primary cause of filling imbalance in geometrically balanced runner system. As a result of experiments, this paper could present an optimal processing condition to minimize variable that brings about filling imbalance geometrically balanced runner system

• 한국수소및신에너지학회논문집
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• 제16권4호
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• pp.356-363
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• 2005

The purpose of this paper is to investigate the optimal condition of the Syngas production by reforming of fuel using plasmatron. Plasma was generated by air and arc discharge. The effects of applied steam, $CO_2$ or Ni-catalyst on fuel conversion, as well as hydrogen yield and $H_2$/CO ratio were studied. When the variations of $O_2$/fuel ratio, $H_2O$/fuel flow ratio and $CO_2$/fuel flow ratio were $0.94{\sim}1.48$, $4.3{\sim}10$ and $0.8{\sim}3.05$, respectively. Under the condition mentioned above, result of $H_2O$/fuel flow ratio was maximum $H_2$ concentration, or $28.2{\sim}31.6%$, and result of $H_2O$/fuel flow ratio with catalyst was minimum CO concentration or $6.6{\sim}7.1%$. and $H_2$/CO ratio were $3.89{\sim}4.86$.

• 한국환경과학회지
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• 제16권10호
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• pp.1203-1208
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• 2007

Feathers are produced in huge quantities as a waste product at commercial poultry processing plants. Since feathers are almost pure keratin protein, feather wastes represent an alternative to more expensive dietary ingredients for animal feedstuffs. Generally they become feather meal used as animal feed after undergoing physical and chemical treatments. These processes require significant energy and also cause environmental pollutions. Therefore, biodegradation of feather by microorganisms represents an alternative method to prevent environment contamination. The aim of this study was to investigate cultural conditions affecting keratinolytic protease production by Bacillus pumilus RS7. We also assessed the nutritive value of microbial and alkaline feather hydrolysates, The composition of optimal medium for the keratinolytic protease was fructose 0.05%, yeast extract 0.3%, NaCl 0.05%, K2HPO4 0.03%, KH2PO4 0.04% and MgCl2 6H2O 0.01%, respectively. The optimal temperature and initial pH was $30^{\circ}C$ and 9.0, respectively. The keratinolytic protease production under optimal condition reached a maximum after 18 h of cultivation. Total amino acid content of feather hydrolysates treated by NaOH and B. pumilius RS7 was $113.8\;{\mu}g/ml$ and $504.9\;{\mu}g/ml$, respectively. Essential amino acid content of feather hydrolysates treated by NaOH and B. pumilius RS7 was $47.2\;{\mu}g/ml$ and $334.0\;{\mu}g/ml$, respectively. Thus, feather hydrolysates have the potential for utilization as an ingredient in animal feed.