• Journal of Microbiology
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• v.42 no.2
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• pp.99-102
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• 2004

Bacteriophage PM2 has a closed circular form of double stranded DNA as a genome. This DNA from the phage is a useful source for nick-circle endonuclease assay in the fmol range. Due to difficulties in the maintenance of viral infectivity, storage conditions of the phage should be considered for the puri-fication of PM2 DNA. The proper condition for a short-term storage of less than 2 months is to keep the PM2 phage at 4$^{\circ}C$; whereas the proper condition for a long-term storage of the PM2 phage for over 2 months is to keep it under liquid nitrogen in 7.5 ％ glycerol. The optimal conditions for a high yield of phage progeny were also considered with the goal to achieve a successful PM2 DNA preparation. A MOI(Multiplicity Of Infection) of 0.03, in which the OD$\sub$600/ of the host bacteria was between 0.3 and 0.5, turned out to be optimal for the mass production of PM2 phage with a burst size of about 214. Considerations of PM2 genome size, and the concentrations and radiospecific activities of purified PM2 DNA, are required to measure the endonuclease activity in the fmol range. This study reports the proper quantitation of radioactivity and the yield of purified DNA based on these conditions.

• Environmental Engineering Research
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• v.20 no.4
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• pp.377-382
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• 2015

The high concentration of nitrogen and phosphorus in wastewater incorporated with the ability to use carbon dioxide as the carbon source make the microalgae become more attractive in wastewater treatment process. This study evaluates the optimal conditions for the digestion of settelable solids from the recirculating aquaculture system to produce the biomass of the green microalga Scenedesmus sp. After solids separation, aerobic digestion of settleable solids under disperse condition produced nitrate as the final product of consequently ammonification and nitrification processes. With the optimal digestion procedure, nitrate concentration during aerobic digestion in 2000 mL vessel increased from $9.63{\pm}0.65mg\;N/L$ to $58.66{\pm}0.06mg\;N/L$ in 10 days. Thereafter, cultivation of Scenedesmus sp. was performed in 1000 mL Duran bottle with air bubbling. The highest Scenedesmus sp. specific growth rate of $0.321{\pm}0.01/d$ was obtained in treatment using liquid fraction after aerobic digestion as the whole culture medium for Scenedesmus sp. cultivation. With this study, digestion of $8,800{\pm}128.12mg\;dry\;weight/L$ of settleable solids from fish pond finally produced $1,235{\pm}21mg\;dry\;weight/L$ of Scenedesmus sp. biomass.

• Journal of the Korean Society of Tobacco Science
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• v.21 no.1
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• pp.17-25
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• 1999

To elucidate the effect of elevated sublethal temperature ($33\pm1^{\circ}C$) on polyamine metabolism and oogenesis, we investigated alterations in the major polyamines and ornithine decarboxylase (ODC) and arginine decarboxylase (ADC), and ovarian development during the pupal-adult development of the tobacco budworm, Helicoverpa assulta. Ovaries ODC activity under the elevated sublethal temperature ($33\pm1^{\circ}C$) were lower than those of the optimal rearing temperature ($25\pm1^{\circ}C$). whereas ovarian ADC activity was consistently higher than the optimal rearing temperature ($25\pm1^{\circ}C$). When the gonads were exposed to the higher temperature, ovarian putrescine showed somewhat suppressed levels throughout development, indicating a relatively high correlationship with the alteration aspects in ODC or ADC activity under elevated sublethal temperature. A somewhat precocious ovary was observed in an early stage of development at $33\pm1^{\circ}C$, but cellular abnormalities occurred in this ovary. The ovary developed under elevated sublethal temperature was observed the inhibitional effect of polyamine metabolism and the abnormal development of ovariole, which seem to be related to the sterility.

• Journal of Dental Anesthesia and Pain Medicine
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• v.17 no.4
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• pp.307-312
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• 2017

Background: The aim of this study was to estimate the optimal depth of nasotracheal tube placement. Methods: We enrolled 110 patients scheduled to undergo oral and maxillofacial surgery, requiring nasotracheal intubation. After intubation, the depth of tube insertion was measured. The neck circumference and distances from nares to tragus, tragus to angle of the mandible, and angle of the mandible to sternal notch were measured. To estimate optimal tube depth, correlation and regression analyses were performed using clinical and anthropometric parameters. Results: The mean tube depth was $28.9{\pm}1.3cm$ in men (n = 62), and $26.6{\pm}1.5cm$ in women (n = 48). Tube depth significantly correlated with height (r = 0.735, P < 0.001). Distances from nares to tragus, tragus to angle of the mandible, and angle of the mandible to sternal notch correlated with depth of the endotracheal tube (r = 0.363, r = 0.362, and r = 0.546, P < 0.05). The tube depth also correlated with the sum of these distances (r = 0.646, P < 0.001). We devised the following formula for estimating tube depth: $19.856+0.267{\times}sum$ of the three distances ($R^2=0.432$, P < 0.001). Conclusions: The optimal tube depth for nasotracheally intubated adult patients correlated with height and sum of the distances from nares to tragus, tragus to angle of the mandible, and angle of the mandible to sternal notch. The proposed equation would be a useful guide to determine optimal nasotracheal tube placement.

• Communications for Statistical Applications and Methods
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• v.15 no.1
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• pp.77-86
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• 2008

This paper develops the optimal periodic preventive maintenance policy following the expiration of non-renewing warranty. We assume that Wu and Clements-Croome's (2005) periodic PM model with random maintenance quality is utilized to maintain the system after the non-renewing warranty is expired. Given the cost structure to the user during the cycle of the product, we drive the expressions for the expected cost rate per unit time. Also, we obtain the optimal number and the optimal period by minimizing the expected cost rate per unit time. The numerical examples are presented for illustrative purpose.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.23 no.12
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• pp.48-57
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• 2009

This paper presents optimal design of direct-driven PM wind generator using MADS (Mesh Adaptive Direct Search). Optimal design of the direct-driven PM Wind Generator, combined with MADS and FEM (Finite Element Method), has been performed to maximize the Annual Energy Production (AEP) over the whole wind speed characterized by the statistical model of the wind speed distribution. In particular, the newly applied MADS contributes to reducing the computation time when compared with Genetic Algorithm (GA) implemented with the parallel computing method.

• Journal of Electrical Engineering and Technology
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• v.3 no.4
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• pp.552-558
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• 2008

Optimal design of the direct-driven Permanent Magnet(PM) wind generator, combined with F.E.A(Finite Element Analysis) and Genetic Algorithm(GA), has been performed to maximize the Annual Energy Production(AEP) over the entire wind speed characterized by the statistical model of wind speed distribution. Particularly, the proposed parallel computing via internet web service has contributed to reducing excessive computing times for optimization.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.6
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• pp.839-848
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• 2015

This study examined the optimal salting drying method and processing conditions (salt concentration, curing time, dry temperature, and drying time) for preparing salted semi-dried common gray mullet (SSD-CGM) Mugil cephalus based on the moisture content, salinity, and overall acceptance using response surface methodology (RSM). The moisture content, salinity, and overall acceptance of SSD-CGM prepared with different salting methods revealed that dry salting was the optimal salting method for preparing high-quality SSD-CGM. The optimal drying method for preparing high-quality SSD-CGM based on the drying velocity and sensory color was hot air-blast drying. The results of the RSM program indicated that the optimal independent variables ($X_1$, salt concentration; $X_2$, curing time; $X_3$, dry temperature; $X_4$, drying time) based on the dependent variables ($Y_1$, moisture content; $Y_2$, salinity; $Y_3$, overall acceptance) for high-quality SSD-CGM were 5.6% for $X_1$, 2.7 h for $X_2$, $47.0^{\circ}C$ for $X_3$, and 8.5 h for $X_4$ for uncoded values. The predicted values of $Y_1$, $Y_2$, and $Y_3$ for SSD-CGM prepared under optimal conditions were 54.4%, 4.2%, and 6.3, respectively, while the experimental values were $55.2{\pm}1.0%$, $4.1{\pm}0.3%$ and $6.7{\pm}0.8$. The actual and predicted values did not differ.

• Proceedings of the Korean Statistical Society Conference
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• 2005.11a
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• pp.73-78
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• 2005

This paper considers a preventive maintenance policy following the expiration of renewing warranty, Most preventive maintenance models assume that each PM costs a fixed predetermined amount regardless of the effectiveness of each PM. However, it seems more reasonable to assume that the PM cost depends on the degree of effectiveness of the PM activity. In this paper we consider a periodic preventive maintenance policy following the expiration of renewing warranty when the PM cost is an increasing function of the PM effect. The optimal number and period for the periodic PM policy with effect dependent cost that minimize the expected cost rate per unit time over an infinite time span are obtained.

• Journal of Korean Society for Quality Management
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• v.35 no.4
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• pp.140-146
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• 2007

In this paper, we consider a periodic imperfect preventive maintenance(PM) policy in which the system's failure rate after each PM remains unchanged. The system undergoes only minimal repairs at failures between PMs. Exact mathematical formula of the expected cost rate per unit time is derived. Optimal number of PMs and optimal maintenance period are derived by minimizing the expected cost rate per unit time. A numerical example is provided to illustrate the proposed approach under Weibull lifetime distribution.