• The Transactions of the Korea Information Processing Society
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• v.7 no.4
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• pp.1211-1216
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• 2000

Asynchronous transfer mode (ATM) can be efficiently used to transport packet data services. The switching system will support voice and packet data services simultaneously from end to end applications. To guarantee quality of service (QoS) of the offered services, source rateot send packet data is needed to control the network overload condition. Most existing control algorithms are shown to provide the threshold-based feedback control technique. However, real-time voice calls can be dynamically connected and released during data services in the network. If the feedback control information delays, quality of the serviced voice can be degraded due to a time delay between source and destination in the high speed link. An adaptive algorithm based on the optimal least mean square error technique is presented for the predictive feedback control technique. The algorithm attempts to predict a future buffer size from weight (slope) adaptation of unknown functions, which are used fro feedback control. Simulation results are presented, which show the effectiveness of the algorithm.

• Journal of Internet Computing and Services
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• v.3 no.3
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• pp.67-74
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• 2002

Asynchronous transfer mode (ATM) can be efficiently used to transport packet data services. The switching system will support voice and packet data services simultaneously from end to end applications. To guarantee quality of service (QoS) of the offered services, source rate to send packet data is needed to control the network overload condition. Most existing control algorithms are shown to provide the threshold-based feedback control technique. However, real-time voice calls can be dynamically connected and released during data services in the network. If the feedback control information delays, quality of the serviced voice can be degraded due to a time delay between source and destination in the high speed link, An adaptive algorithm based on the optimal least mean square error technique is presented for the predictive feedback control technique. The algorithm attempts to predict a future buffer size from weight (slope) adaptation of unknown functions, which are used for feedback control. Simulation results are presented, which show the effectiveness of the algorithm.

• Journal of Microbiology and Biotechnology
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• v.18 no.8
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• pp.1386-1392
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• 2008

A gene (sll0158) putatively encoding a glycogen branching enzyme (GBE, E.C. 2.4.1.18) was cloned from Synechocystis sp. PCC6803, and the recombinant protein expressed and characterized. The PCR-amplified putative GBE gene was ligated into a pET-21a plasmid vector harboring a T7 promoter, and the recombinant DNA transformed into a host cell, E. coli BL21(DE3). The IPTG-induced enzymes were then extracted and purified using Ni-NTA affinity chromatography. The putative GBE gene was found to be composed of 2,310 nucleotides and encoded 770 amino acids, corresponding to approx. 90.7 kDa, as confirmed by SDS-PAGE and MALDI-TOF-MS analyses. The optimal conditions for GBE activity were investigated by measuring the absorbance change in iodine affinity, and shown to be pH 8.0 and $30^{\circ}C$ in a 50 mM glycine-NaOH buffer. The action pattern of the GBE on amylose, an $\alpha$-(1,4)-linked linear glucan, was analyzed using high-performance anion-exchange chromatography (HPAEC) after isoamylolysis. As a result, the GBE displayed $\alpha$-glucosyl transferring activity by cleaving the $\alpha$-(1,4)-linkages and transferring the cleaved maltoglycosyl moiety to form new $\alpha$-(1,6)-branch linkages. A time-course study of the GBE reaction was carried out with biosynthetic amylose (BSAM; $M_p{\cong}$8,000), and the changes in the branch-chain length distribution were evaluated. When increasing the reaction time up to 48 h, the weight- and number-average DP ($DP_w$ and $DP_n$) decreased from 19.6 to 8.7 and from 17.6 to 7.8, respectively. The molecular size ($M_p$, peak $M_w{\cong}2.45-2.75{\times}10^5$) of the GBE-reacted product from BSAM reached the size of amylose (AM) in botanical starch, yet the product was highly soluble and stable in water, unlike AM molecules. Thus, GBE-generated products can provide new food and non-food applications, owing to their unique physical properties.