• 대한수의학회지
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• 제30권3호
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• pp.297-307
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• 1990

To establish an agar-gel immunodiffusion (AGID) test for detection of antibodies to Aujeszky's disease virus(ADV) in swine, the precipitating antigens were prepared by four procedures using the Aujeszky's disease virus, NYJ-1-87 strain isolated from the affected piglets in Korea. The optimal condition for AGID test and the properties of the antigens were investigated. To determine the optimal concentration of antigens, four antigens were experimentally prepared by concentrating the viral fluids by 1/30 to 1/200. It was proved that the antigen precipitated with ammonium sulfate at concentration of 1/100 was the most efficient to detect ADV antibodies by AGID test. When the relationship between the concentration of the antigens and the size of precipitating in radial immunodiffusion test was investigated, a high correlation coefficiency at r=0.95 (y=0.23x+23.4) was estimated, In study on the effects of various buffered salt solutions and agars on the sensitivity of AGID test by using the experimental ADV antigens, it was found that 0.05M tris buffer without sodium chloride at pH 7.2 induced the most distinctive precipitating lines, and that there was no significant differences in the sensitivity between the agarose and Noble's special agar. When the efficiency of AGID test was compared with serum neutralization(SN) test, the sensitivity of AGID test was 100% in SN titer over 1 : 16, 91.7% in SN titer of 1 : 8 and 57.1% in SN titer of 1 : 4. The specificity of AGID test compared with the sera with SN titer under 1 : 2 was 98.4%. Protein analysis of the antigens by SDS-PAGE indicated that antigen I and antigen III showed a specific band of polypeptides with molecular weight of 116 K in comparison with the control antigen. Antigen IV, treated with tween-80 and ammonium sulfate, revealed specific polypeptides bands at the molecular weights 45K, 98K and 150 K.

• Journal of Microbiology and Biotechnology
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• 제23권6호
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• pp.864-871
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• 2013

The recombinant strain P. pastoris GS115-lccC was used to produce laccase with high activity. Factors influencing laccase expression, such as pH, methanol concentration, copper concentration, peptone concentration, shaker rotate speed, and medium volume were investigated. Under the optimal conditions, laccase activity reached 12,344 U/L on day 15. The recombinant enzyme was purified by precipitating and dialyzing to electrophoretic homogeneity, and was estimated to have a molecular mass of about 58 kDa. When guaiacol was the substrate, the laccase showed the highest activity at pH 5.0 and was stable when the pH was 4.5~6.0. The optimal temperature for the laccase to oxidize guaiacol was $60^{\circ}C$, but it was not stable at high temperature. The enzyme could remain stable at $30^{\circ}C$ for 5 days. The recombinant laccase was used to degrade chlorpyrifos in several laccase/mediator systems. Among three synthetic mediators (ABTS, HBT, VA) and three natural mediators (vanillin, 2,6-DMP, and guaiacol), vanillin showed the most enhancement on degradation of chlorpyrifos. Both laccase and vanillin were responsible for the degradation of chlorpyrifos. A higher dosage of vanillin may promote a higher level of degradation of chlorpyrifos, and the 2-step addition of vanillin led to 98% chlorpyrifos degradation. The degradation of chlorpyrifos was faster in the L/V system ($k_{obs}$ = 0.151) than that in the buffer solution ($k_{obs}$ = 0.028).

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제44회 동계 정기학술대회 초록집
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• pp.183-184
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• 2013

Two main MBE growth techniques have been used: plasma-assisted MBE (PA-MBE), which utilizes a rf plasma to supply active nitrogen, and ammonia MBE, in which nitrogen is supplied by pyrolysis of NH3 on the sample surface during growth. PA-MBE is typically performed under metal-rich growth conditions, which results in the formation of gallium droplets on the sample surface and a narrow range of conditions for optimal growth. In contrast, high-quality GaN films can be grown by ammonia MBE under an excess nitrogen flux, which in principle should result in improved device uniformity due to the elimination of droplets and wider range of stable growth conditions. A drawback of ammonia MBE, on the other hand, is a serious memory effect of NH3 condensed on the cryo-panels and the vicinity of heaters, which ruins the control of critical growth stages, i.e. the native oxide desorption and the surface reconstruction, and the accurate control of V/III ratio, especially in the initial stage of seed layer growth. In this paper, we demonstrate that the reliable and reproducible growth of GaN on Si (110) substrates is successfully achieved by combining two MBE growth technologies using rf plasma and ammonia and setting a proper growth protocol. Samples were grown in a MBE system equipped with both a nitrogen rf plasma source (SVT) and an ammonia source. The ammonia gas purity was ＞99.9999% and further purified by using a getter filter. The custom-made injector designed to focus the ammonia flux onto the substrate was used for the gas delivery, while aluminum and gallium were provided via conventional effusion cells. The growth sequence to minimize the residual ammonia and subsequent memory effects is the following: (1) Native oxides are desorbed at $750^{\circ}C$ (Fig. (a) for [$1^-10$] and [001] azimuth) (2) 40 nm thick AlN is first grown using nitrogen rf plasma source at $900^{\circ}C$ nder the optimized condition to maintain the layer by layer growth of AlN buffer layer and slightly Al-rich condition. (Fig. (b)) (3) After switching to ammonia source, GaN growth is initiated with different V/III ratio and temperature conditions. A streaky RHEED pattern with an appearance of a weak ($2{\times}2$) reconstruction characteristic of Ga-polarity is observed all along the growth of subsequent GaN layer under optimized conditions. (Fig. (c)) The structural properties as well as dislocation densities as a function of growth conditions have been investigated using symmetrical and asymmetrical x-ray rocking curves. The electrical characteristics as a function of buffer and GaN layer growth conditions as well as the growth sequence will be also discussed. Figure: (a) RHEED pattern after oxide desorption (b) after 40 nm thick AlN growth using nitrogen rf plasma source and (c) after 600 nm thick GaN growth using ammonia source for (upper) [110] and (lower) [001] azimuth.

• 제어로봇시스템학회논문지
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• 제3권4호
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• pp.381-388
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• 1997

This paper proposes a heuristic algorithm to efficiently perform line balancing in the PCB assembly line including multiple surface mounters efficiently. Generally, the problems in line balancing are classified into two kinds. Firstly, is the determining of the minimum number of machines required for achieving the desired production rate. Secondly, is the assign of jobs to multiple machines in order to minimize the cycle time which is defined as a maximum among the working times of machines when the number of machines is fixed. In this paper, we deal with the latter. We consider a PCB assembly line, including the multiple surface mounters arranged serially as a target system. Also, the conveyor is assumed to move at a constant speed and have no buffer. Considering that the minimum number of machines required for the desired production rate is a discrete nonincreasing function which is inversely proportional to the cycle time, we propose an optimization algorithm for line balancing by using the binary search method. The algorithm is validated through computer simulation, the results of which show that their shapes coincide nearly with those of optimal line balancing efficiency graphs regardless of the number of components, the performance of surface mounters, and the structure of assembly line.

• 대한전자공학회논문지TC
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• 제40권7호
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• pp.261-272
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• 2003

Ethernet PON(Passive Optical Network)은 최근 활발하게 연구되고 있는 경제적이고 효율적인 가입자망이다. 차세대 가입자망인 Ethernet PON의 MAC(Media Access Control) 프로토콜은 TDMA(Time Division Multiple Access)방식을 기본으로 하며, QoS(Qualify of Service)를 보장해야 하는 전용회선(Leased Line) 가입자에 적합한 고정 할당 방식과 LAN/MAN의 최선형(Best Effort) 방식에 적합한 동적 할당 방식으로 나눌 수 있다. 본 논문에서는 이들 프로토콜의 성능을 분석하기 위하여 OPNET 시뮬레이션 도구를 이용해 Ethernet PON 모델을 설계한다. 설계된 모델에 대한 단대단 Ethernet 지연(end-to-end Ethernet delay), 큐잉 지연(queuing delay), 처리(throughput)과 사용률(utilization) 분석을 통해서 MAC 프로토콜을 검증하고, 하나의 OLT(Optical Line Terminal)가 수용할 수 있는 최적의 ONU(Optical Network Unit) 개수를 산정한다. 또한, 적정한 ONU의 버퍼 크기를 제안하여 Ethernet PON의 망 효율을 극대화하는 방안을 제시한다.

• 대한본초학회지
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• 제39권3호
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• pp.69-76
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• 2024

Objectives : Hypertension (high blood pressure), one of the world's major chronic diseases, has a high mortality rate due to its high prevalence and complications, but its control rate is low. The proper management and control through appropriate exercise, diet management, and optimal drug choice can reduce the risk of death from hypertension. Although various antihypertensive drugs are used to treat hypertension, they also have numerous adverse effects. Alongside increased interest in the use of Traditional Herbal Formulas (THF) for hypertension treatment, the purpose of this study was to examine the vasodilative effects of 10 THF in the rat thoracic artery pre-contracted by potassiumchloride (KCl). Methods : THF were extracted with distilled water for 2 hours. The rat thoracic artery was suspended and contracted by KCl in the organ bath which contained 10 ml Krebs Henseleit (KH) buffer. THF extracts were added in a dose-dependent increase (10-1,000 ㎍/mL) to examine vasodilative effects. The vasodilative effects produced by THF were expressed as the percentage in response to KCl-induced contraction. Results : Among the 10 THF, Banhasasim-tang, Buhnsimgieum, Sagunja-tang, and Samul-tang showed vasodilative effects. And, Sipjeondaebo-tang, Ssanghwa-tang, Ojeok-san, Onkyung-tang, Yongdamsagan-tang, and Hyangsayukgunja-tang showed no significant vasodilative effects. Also, in co-administration with amlodipine, Banhasasim-tang showed higher vasodilative effects than amlodipine alone, and Buhnsimgieum showed greater vasodilative effects at low concentrations, but inhibited amlodipine's vasodilative effects at high concentrations. Conclusion : As a result of these studies, they will be expected to provide useful data to establish guidelines of combined administration of THF and western antihypertensive drugs for the treatment of hypertension.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2003년도 추계학술발표강연 및 논문개요집
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• pp.235-235
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• 2003

The development of a buffer layer is an important issue for the second -generation wire, YBCO coated metal wire. The buffer layer demands not only on the prohibition of the reaction between YBCO and metal substrate, but also the proper lattice match and conductivity for high critical current density (Jc) of YBCO superconductor, In order to satisfy these demands, we suggested CaRuO3 as a useful candidate having that the lattice mismatches with Ni (200) and with YBCO are 8.2% and 8.0%, respectively. The CaRuO3 thin films were deposited on Ni substrates using various methods, such as e-beam evaporation and DC and RF magnetron sputtering. These films were investigated using SEM, XRD, pole-figure and AES. In e-beam evaporation, the deposition temperature of CaRuO3 was the most important since both hi-axial texturing and NiO formation between Ni and CaRuO3 depended on it. Also, the oxygen flow rate had i[n effect on the growth of CaRuO3 on Ni substrates. The optimal conditions of crystal growth and film uniformity were 400$^{\circ}C$, 50 ㎃ and 7 ㎸ when oxygen flow rate was 70∼100sccm In RF magnetron sputtering, CaRuO3 was deposited on Ni substrates with various conditions and annealing temperatures. As a result, the conductivity of CaRuO3 thin films was dependent on CaRuO3 layer thickness and fabrication temperature. We suggested the multi-step deposition, such as two-step deposition with different temperature, to prohibit the NiO formation and to control the hi-axial texture.

• 한국통신학회논문지
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• 제25권8A호
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• pp.1160-1168
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• 2000

본 논문은 ATM 네트워크에서 예측하기 힘든 트래픽 특성을 갖는 VBR 및 ABR 서비스를 효과적으로 관리하여 서비스 품빌을 지원할 수 있는 실시간 통합트랙픽 관리(RITM : real-time integrated traffic management) 기법을 제안한다. RITM 기법은 독특한 ATM 셀 제어 블록을 운용하여 버스트 특성을 갖는 트래픽을 실시간으로감시하여 셀 도착 율의측정 시 소요되는 지연 시간을 최소화하고 기존의 연결들의 서비스 품질에 영향을 미치지않도록 여분의 네트워크 자원을 동적으로 재 할당하도록 한다 컴퓨터 시뮬레이션을 통하여 제안한 RITM 기법이 입력 트래픽을 정확히 감시하고 네트워크 자원을 효율적으로 관리함을 보였으며 이는 주어진 네트워크 상황에서 수용가능한 최적의 사용자 연결수를 결정할수 있음을 증명한다 마지막으로 이 연결 값이 가격 대비 성능을 최대화할 수 있는 버퍼 설계 및 네트워크 혼잡 상태를 방지하는 임계치로 사용될 수 있음을 보였다.

• Journal of Microbiology and Biotechnology
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• 제25권10호
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• pp.1742-1750
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• 2015

Human insulin is composed of 21 amino acids of an A-chain and 30 amino acids of a B-chain. This is the protein hormone that has the role of blood sugar control. When the recombinant human proinsulin is expressed in Escherichia coli, a serious problem is the formation of an inclusion body. Therefore, the inclusion body must be denatured and refolded under chaotropic agents and suitable reductants. In this study, H27R-proinsulin was refolded from the denatured form with β-mercaptoethanol and urea. The refolding reaction was completed after 15 h at $15^{\circ}C$, whereas the reaction at $25^{\circ}C$ was faster than that at $15^{\circ}C$. The refolding yield at $15^{\circ}C$ was 17% higher than that at $25^{\circ}C$. The refolding reaction could be carried out at a high protein concentration (2 g/l) using direct refolding without sulfonation. The most economical and optimal refolding condition for human preproinsulin was 1.5 g/l protein, 10 mM glycine buffer containing 0.6 M urea, pH 10.6, and 0.3 mM β-mercaptoethanol at $15^{\circ}C$ for 16 h. The maximum refolding yield was 74.8% at $15^{\circ}C$ with 1.5 g/l protein. Moreover, the refolded preproinsulin could be converted into normal mature insulin with two enzymes. The average amount of human insulin was 138.2 g from 200 L of fermentation broth after enzyme reaction with H27R-proinsulin. The direct refolding process for H27R-proinsulin was successfully set up without sulfonation. The step yields for refolding and enzyme reaction were comparatively high. Therefore, our refolding process for production of recombinant insulin may be beneficial to the large-scale production of other biologically active proteins.

• Architectural research
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• 제22권2호
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• pp.41-52
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• 2020

Laboratory buildings with specialized equipment and ventilation systems pose challenges in terms of efficient energy use and initial construction costs. Additionally, lab spaces should have flexible and efficient layouts and provide a comfortable indoor research environment. Therefore, this study aims to identify the correlation between the facade of a building and its interior layout from case studies of energy-efficient research labs and to propose passive energy design strategies for the establishment of an optimal research environment. The case studies in this paper were selected from the American Institute of Architects Committee on the Environment Top Ten Projects and Leadership in Energy and Environmental Design (LEED) certified research lab projects. In this paper, the passive design strategies of space zoning, façade design devices to control heating and cooling loads were analyzed. Additionally, the relationships between these strategies and the interior lab layouts, lab support spaces, offices, and circulation areas were examined. The following four conclusions were drawn from the analysis of various cases: 1) space zoning for grouping areas with similar energy requirements is performed to concentrate similar heating and cooling demands to simplify the HVAC loads. 2) Public areas such as corridor, atrium, or courtyard can serve as buffer zones that employ passive solar design to minimize the mechanical energy load. 3) A balanced window-to-wall ratio (WWR), exterior shading devices, and natural ventilation systems are applied according to the space programming energy requirements to minimize the dependence on mechanical service. 4) Lastly, typical laboratory space zoning categories can be revised, reversed, and even reconfigured to minimize the energy load and adjust to the site context. This study can provide deep insights into various design strategies employed for construction of green laboratories along with intuitive arrangement of various building components such as laboratory spaces, lab support spaces, office spaces, and common public areas. The key findings of this study can contribute towards creating improved designs of laboratory facilities with reduced carbon footprint and greenhouse emissions.