• 제목/요약/키워드: Optical microscope

검색결과 1,386건 처리시간 0.086초

High Resolution Photonic Force Microscope Using Resonance Energy Transfer

  • Heo, Seung-Jin;Kim, Ki-Pom;Cho, Yong-Hoon
    • 한국진공학회:학술대회논문집
    • /
    • 한국진공학회 2010년도 제39회 하계학술대회 초록집
    • /
    • pp.288-288
    • /
    • 2010

  • Photonic Force Microscope (PFM) is a scanning force microscope using an optical trap with several piconewton. In PFM, we can have topological information from the bead position trapped in optical trap. Typically the resolutions of lateral and vertical position are 40 nm and 50 nm respectively. To improve the vertical resolution below 10 nm, we use resonance energy transfer which has 5nm resolution in distance. Here we show preliminary results, including performances of scanning bead and fluorescence imaging system.

  • PDF

Statistical Analysis of Fluorescence Correlation Spectroscopy of Ultra Low Concentration Molecules with a Confocal Microscope

  • Lee, Soon-Hyouk;Lim, Gyu-Chang;Kim, Soo-Yong;Kim, Eun-Kyung;Kim, Hak-Sung;Kim, Sok-Won
    • Journal of the Optical Society of Korea
    • /
    • 제12권3호
    • /
    • pp.170-173
    • /
    • 2008

  • In this study, we simulated a statistical model of FCS(fluorescence correlation spectroscopy) based on a Poisson process to understand and explain observations of the experiment performed on molecules of ultra-low concentration by the home-built laser-scanning confocal microscope. The statistical model confirmed that the relative mean square amplitude of fluctuations is shown to be inversely proportional to the average number of molecules, even in the ultra-low concentration, if some conditions are satisfied. Signal-to-noise ratio and the variability of dwelling time under the confocal volume were found to be effective conditions for the experiment.

  • https://doi.org/10.3807/JOSK.2008.12.3.170 인용 PDF KSCI