• Title/Summary/Keyword: Optical microscope

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High Resolution Photonic Force Microscope Using Resonance Energy Transfer

  • Heo, Seung-Jin;Kim, Ki-Pom;Cho, Yong-Hoon
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.08a
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    • pp.288-288
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    • 2010

  • Photonic Force Microscope (PFM) is a scanning force microscope using an optical trap with several piconewton. In PFM, we can have topological information from the bead position trapped in optical trap. Typically the resolutions of lateral and vertical position are 40 nm and 50 nm respectively. To improve the vertical resolution below 10 nm, we use resonance energy transfer which has 5nm resolution in distance. Here we show preliminary results, including performances of scanning bead and fluorescence imaging system.

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Statistical Analysis of Fluorescence Correlation Spectroscopy of Ultra Low Concentration Molecules with a Confocal Microscope

  • Lee, Soon-Hyouk;Lim, Gyu-Chang;Kim, Soo-Yong;Kim, Eun-Kyung;Kim, Hak-Sung;Kim, Sok-Won
    • Journal of the Optical Society of Korea
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    • v.12 no.3
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    • pp.170-173
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    • 2008

  • In this study, we simulated a statistical model of FCS(fluorescence correlation spectroscopy) based on a Poisson process to understand and explain observations of the experiment performed on molecules of ultra-low concentration by the home-built laser-scanning confocal microscope. The statistical model confirmed that the relative mean square amplitude of fluctuations is shown to be inversely proportional to the average number of molecules, even in the ultra-low concentration, if some conditions are satisfied. Signal-to-noise ratio and the variability of dwelling time under the confocal volume were found to be effective conditions for the experiment.

  • https://doi.org/10.3807/JOSK.2008.12.3.170 인용 PDF KSCI