• Title/Summary/Keyword: Optical microscope

Search Result 1,386, Processing Time 0.062 seconds

Scanning Confocal Microscope by direct drive of optical fiber (광섬유의 직접구동에 의한 공초점 현미경)

  • 박두성;류광현;노정은;김종배;권남익
    • Proceedings of the Optical Society of Korea Conference
    • /
    • 2002.07a
    • /
    • pp.80-81
    • /
    • 2002
  • 편광 방향이 단일 모드의 유지되는 광섬유와 파장이 780nm인 반도체 레이저를 사용하여 간결한 구조의 beam scanning 타입의 공초점 현미경을 구성하였다. 공초점 현미경은 세포내의 생명 현상을 보다 잘 이해 할 수 있는 장비이기 때문에 기존의 현미경으로는 연구할 수 없던 생명현상을 연구할 수 있도록 여러 가지 강력한 연구수단을 제공하고 있다 살아 움직이는 생명체를 관찰할 수 있는 공초점 현미경에서 중요한 것은 속도와 투과 깊이이고, 장기의 표면을 관찰하기 위해서는 크기가 작아야 한다. (중략)

  • PDF

Surface profile measurement with optically trapped micro-particles (광포획된 마이크로 입자를 이용한 표면형상 측정)

  • 주지영;김준식;김승우
    • Proceedings of the Optical Society of Korea Conference
    • /
    • 2001.02a
    • /
    • pp.116-117
    • /
    • 2001
  • 정밀 삼차원 미세 형상 측정기에서 성능의 관건은 고속, 고분해능으로 측정하는 것이다. 그러기 위해서는 공진주파수가 높아야 하고 스프링 상수가 작아야 한다. 광포획 현미경(optical trap microscope, OTM)은 광포획 된 마이크로 입자를 프로브로 사용하는 것으로 입자에 작용하는 복원력이 광에 의한 힘뿐이므로 스프링 상수가 낮다. 또한 공진주파수는 f=√k/m 으로 입자의 질량이 매우 작으므로 공진주파수도 비교적 높다. (중략)

  • PDF

Characterization of $ZrO_2$ thin films fabricated by glancing angle deposition

  • Sobahan, K.M.A;Park, Yong-Jun;HwangBo, Chang-Kwon
    • Proceedings of the Optical Society of Korea Conference
    • /
    • 2008.02a
    • /
    • pp.281-282
    • /
    • 2008
  • The glancing angle deposition (GLAD) technique was used to fabricate $ZrO_2$ thin films by electron-beam evaporation. The crystal structure, cross-sectional structure, surface morphology and optical properties are characterized by X-ray diffraction meter (XRD, Rigaku, Cu $K{\alpha}$ - radiation), scanning electron microscope (SEM), and spectrophotometer, respectively.

  • PDF

Observation of Multiple Filamentation in a-As$_2$S$_3$ film Using a Near-Field Scanning Optical Microscope (근접장 광학 현미경을 이용한 비정질 $AS_2$$S_3$ 박막에서의 다중 필라멘테이션 관찰)

  • 정희성;황성태;조규만
    • Proceedings of the Optical Society of Korea Conference
    • /
    • 2003.07a
    • /
    • pp.182-183
    • /
    • 2003
  • 자체집광(Self-focusing)현상은 매우 높은 집적도를 가지는 광기록매체의 제작에 응용 가능하기 때문에 많은 연구자들에 의해 광범위하게 연구되어왔다. 그동안의 연구에서는 자체집광에 대한 분석방법으로서 빛살의 벡터특성을 무시한 비선형 슈뢰딩거 방정식(nonlinear Schr dinger equation)을 이용하였는데, 이 경우 축상으로 대칭적인 빛살이 입사될 때 발생되는 다중 필라멘테이션(Multiple Filamentation)현상은 입사빛살에 첨가되는 random noise에 기인한다는 것이 유일한 해석방법이었다. (중략)

  • PDF

Investigation of self-focusing effect using near field scanning optical microscope (근접장 주사광학현미경을 이용한 자체집광현상에 관한 연구)

  • 유장훈;임상엽;양재석;이현호;주홍렬;박승한;이범구
    • Proceedings of the Optical Society of Korea Conference
    • /
    • 2003.07a
    • /
    • pp.190-191
    • /
    • 2003
  • 물질에 큰 전기장을 가하였을 때 그 물질의 전기 감수율이 선형이 아닌 비선형관계를 갖게 되는데, 이로 인하여 선형계에서는 볼 수 없는 여러 가지 특이한 현상들이 관측된다. 이러한 비선형적인 현상 중에서 자체집광현상은 Guassian세기 분포를 가지는 빛을 매질에 통과시켰을 때 시료가 얇은 렌즈처럼 작용하는 것으로 설명할 수 있다. 비선형 효과에 의하여 레이저빔이 본래의 특성과 달라질 수 있고 빔의 심각한 변형을 일어난다. (중략)

  • PDF

Microscope Examination of Attached Biofilm under Anaerobic Conditions (혐기성 조건에서 담체에 부착된 미생물의 관찰)

  • 박성열;김도한;나영수;박영식;송승구
    • Journal of Environmental Health Sciences
    • /
    • v.27 no.1
    • /
    • pp.100-105
    • /
    • 2001
  • Microstructural examinations were performed on the anaerobic biofilm from reactor filled with PE support media. Optical microscope, SEM and fluorescent microscope were used for qualitative and morphological studies on the attached microorganism under anaerobic condition. Microorganisms were attached in crevices where protection from shear forces of surfaces where easy to contact with support media surface. A hypothesis for biofilm accumulation occurs on a surface such as polymer support media is presented schematically : 1st step ; cell-support media attachment, 2nd step ; cell-support media attachment and cell-cell attachment, 3rd step ; attached biofilm from neighboring crevices joins together and growing, 4th step ; mature and irregualar biofilm was formed. In SEM photographs, shape and structures of biofilm were observed, but microorganism species and methanogens were not identified. A large number of methanogenic bacteria were identified on the surface of PE substratum by fluorescence under 480nm of radiation and it was estimated that methanogenic bacteria was related to initial attachment of bacteria under anaerobic condition.

  • PDF

Design and Control of Mini-Scanning Electron Microscope (미니형 주사전자 현미경의 설계 및 제어)

  • Park, Man-Jin;Kim, Dong-Hwan;Kim, Young-Dae;Jang, Dong-Young;Han, Dong-Chul
    • Proceedings of the KSME Conference
    • /
    • 2007.05a
    • /
    • pp.1271-1276
    • /
    • 2007
  • The most powerful analytical equipment usually comes at the cost of having the highest demand for space. Where electron microscopes has traditionally required a room to themselves, not just for reasons of their size but because of ancillary demands for pipes and service. The simple optical microscopes, of course, can occupy the desk-top, but because their performance is limited by the wavelength of light, their powers of magnification and resolution are inferior to that of the electron microscope. Mini SEM will sit comfortably on a desk-top but offers magnification and resolution performances much closer to that of a standard SEM. This new technique extends the scope of SEM as a high-resolution microscope, relatively cheap and widely available imaging tool, for a wider variety of samples.

  • PDF

The Study on the Phase Transition of Langmuir Film by Brewster-Angle Microscope (BAM(Brewster-Angle Microscope)으로 관측한 Langmuir막의 상전이에 관한 연구)

  • Cho, Wan-Je;Song, Kyung-Ho;Park, Tae-Gone;Park, Keun-Ho
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
    • /
    • 2000.05b
    • /
    • pp.323-326
    • /
    • 2000
  • In this study, we used Brewster-Angle Microscope(BAM) to study on the molecular orientation of monolayer on the water surface. The behaviors of molecules on three different subphase have been observed. Reproducible $\pi$-A isotherm have been obtained only on information about phase transition by molecular area. BAM facilitates the observation of morphology by optical anisotropy and thickness in monolayer and multilayers as BAM is shown to be sensitive to anisotropy of film. Every transition was found by BAM technique, either as a dramatic change in degree of contrast or as a sudden alteration of molecular action and $\pi$-A isotherm.

  • PDF

Design and Fabrication of a Vacuum Chamber for a Commercial Atomic Force Microscope

  • Park, Sang-Joon;Jeong, Yeon-Uk;Park, Soyeun;Lee, Yong Joong
    • Applied Science and Convergence Technology
    • /
    • v.23 no.2
    • /
    • pp.97-102
    • /
    • 2014
  • A vacuum chamber for a commercial atomic force microscope (AFM) is designed and fabricated. Only minimal modifications were made to an existing microscope in an effort to work in a vacuum environment, while most of the available AFM functionalities were kept intact. The optical alignment needed for proper AFM operations including a SLD (superluminescent diode) and a photodiode can be made externally without breaking the vacuum. A vacuum level of $5{\times}10^{-3}$ torr was achieved with a mechanical pump. An enhancement of the quality factor was observed along with a shift in the resonance frequency of a non-contact-mode cantilever in a vacuum. Topographical data of a calibration sample were also obtained in air and in a low vacuum using the non-contact mode and the results were compared.