• 제목/요약/키워드: Optical Microscopy

검색결과 1,429건 처리시간 0.025초

Dark Field Digital Holographic Microscopy Based on Two-lens 360-degree Oblique Illumination

  • Zhang, Xiuying;Zhao, Yingchun;Yuan, Caojin;Feng, Shaotong;Wang, Lin
    • Current Optics and Photonics
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    • 제4권3호
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    • pp.193-199
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    • 2020
  • In this paper we propose a dark-field digital holographic microscopy system based on 360-degree oblique illumination. This setup is constructed without using a dark-field objective. The principle of 360-degree oblique illumination of vortex beam and dark-field digital holographic microscopy are introduced theoretically and experimentally. By analyzing the reconstructed image of a dark-field digital hologram of a USAF 1951 target, it is proved that the imaging resolution can be improved by this method. And also, comparison and analysis are made on the reconstructed image of a bright-dark field digital hologram of a pumpkin stem slice, the result shows that the imaging contrast is also enhanced with this method, and it is effective for dark-field digital holographic microscopy imaging of large transparent biological samples.

Instrumental Methods for Differentiation of Frozen-thawed from Fresh Broiler Breast Fillets

  • Jung, Samooel;Lee, Jae-Cheong;Jung, Yeon-Kuk;Kim, Min-Kyu;Son, Hwa-Young;Jo, Cheo-Run
    • 한국축산식품학회지
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    • 제31권1호
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    • pp.27-31
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    • 2011
  • To differentiate between frozen-thawed and fresh broiler breast fillets, different methods such as optical microscopy and measurement of drip loss, pH, torrymeter and K-value were performed. A total of 10 samples of fresh and frozen-thawed breast fillets were stored in a refrigerator ($4^{\circ}C$) for 5 d. Optical microscopy of the frozen-thawed breast fillets found structural changes caused by ice crystals, which may have significantly increased drip loss compared to fresh breast fillet. The pH and K-value could not be distinguished between the two breast fillets during storage. However, the torrymeter values of the fresh and frozen-thawed breast fillets were significantly different (p<0.05). The results indicate that both optical microscopy and torrymeter measurement can be effective methods for differentiating between fresh and frozen-thawed breast fillets. However, optical microscopy may be difficult to implement in the marketplace since it requires much time and effort. Thus, the determination of the torrymeter value is the easiest and most rapid instrumental method among those tested for the differentiation of frozen-thawed chicken breast fillet from fresh one.

고밀도 광학헤드를 위한 Bimorph 압전 액추에이터의 동특성 해석 및 위치제어 (Dynamic Characteristic Analysis and Position Control for High Density Optical Head Using Bimorph PZT)

  • 박태욱;박노철;양현석;박영필;권영기
    • 한국소음진동공학회논문집
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    • 제15권1호
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    • pp.12-19
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    • 2005
  • This paper proposed a dual actuator using Bimorph PZT for information storage device based on prove array NSOM(near-field scanning optical microscopy). The gap between the media and the optical head should be maintained within the optical tolerance. Therefore, a new actuator having high sensitivity is required. Bimorph PZT, which has fast access time and high sensitivity characteristic, is suitable for this precise actuating system. This paper is focused on derivation of mathematical model of dual Bimorph PZT actuator and control algorithm. Hamilton's principle was used for mathematical model. The model is verified by FEA(finite element analysis), and compared with experimental results. Different control algorithms were used for two Bimorph PZT actuating same direction and opposite direction. The gap between recording media and optical head was controlled within 20nm in experiment.

투과전자현미경을 이용한 상전이형 광디스크의 미세조직 관찰 (Microstructural Observation of Phase Change Optical Disk by TEM)

  • 김수철;김긍호
    • Applied Microscopy
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    • 제29권4호
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    • pp.493-498
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    • 1999
  • 기억저장매체로서 광디스크의 개발이 활발히 진행되고 있으며 최근의 레이저 기술의 발전, 제조기술의 발전에 따른 기억밀도의 증가로서 기존의 자기기록매체와 경쟁을 하고 있다. 기록밀도의 증대와 신뢰성의 향상을 위하여 다층박막 구조를 가지는 광디스크의 미세구조는 더욱 복잡하며 소형화되고 있다. 이종의 물질로 구성된 다층박막형 광디스크의 미세구조 관찰 및 분석을 위해서는 투과전자현미경과 같은 미소영역 분석법이 필수적이며 비교적 간단하고 신뢰성 높은 시편준비법의 확립이 선행되어야 한다. 본 연구에서는 투과전자현미경 분석을 위한 광디스크의 평면 및 단면시료 제작법을 제시하고 제조된 시편으로부터 얻어진 광디스크의 미세구조 분석결과를 보고한다.

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Development and Evaluation of an Electron Beam Source for Microscopy and Its Applications

  • Ahn, Seung-Joon;Oh, Tae-Sik;Kim, Ho-Seob;Ahn, Seong-Joon
    • Journal of the Optical Society of Korea
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    • 제14권2호
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    • pp.127-130
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    • 2010
  • We have developed an efficient electron beam (e-beam) source, a microcolumn, that can be used as a source module for of microscopy and its applications. To obtain a low operating voltage, a very sharp cold field electron emitter was developed by electrochemically etching a tungsten wire. Laser diffraction was used for the fabrication of high-quality electron lenses and for their precise alignment. The measurement of the e-beam currents, and SEM images captured by the microcolumn confirmed the potential of the device as a very good e-beam source.

Emulsion과 Solution SBR Compound에 있어서 혼합시간(混合時間)에 따른 Carbon Black분산(分散)의 분석방법(分析方法)들 사이의 비교연구(比較硏究) (A Comparative Study Characterization Methods of Carbon Black Dispersion in Solution and Emulsion SBR Compounds Prepared at Various Mixing Levels)

  • 이성덕
    • Elastomers and Composites
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    • 제24권3호
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    • pp.193-202
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    • 1989
  • An experimental study of the development of carbon black dispersion by an internal mixer and its characterization is presented. We describe the measurement of carbon black agglomerate size and related characteristics using four different experimental techniques : optical microscopy, scanning elctrion microscopy, surface roughness, and electrical couductivity. The results from these different experiments are compared uning the same carbon black for a series of six different butadiene-styrene copolymers. The results from the different techniques are cross plotted and are critically discussed. It is found that surface but then sharply deteriorate. At subsequent stages of mixing optical microscopy seemed the most reliable measure.

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Improving Phase Contrast of Digital Holographic Microscope using Spatial Light Modulator

  • Le, Thanh Bang;Piao, Meilan;Jeong, Jong-Rae;Jeon, Seok-Hee;Kim, Nam
    • Journal of the Optical Society of Korea
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    • 제19권1호
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    • pp.22-28
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    • 2015
  • We propose a new method for improving the phase contrast of a multiphase digital holographic microscope using a spatial light modulator (SLM). Using the SLM as the annulus, our method improves the light contrast of the object edge to achieve higher accuracy. We demonstrate a digital holographic microscopy technique that provides a 30% improvement in the phase contrast compared to conventional microscopy, which utilizes a mechanical annulus. The phase-contrast improvement allows the 3D reconstructed hologram to be determined more precisely.

Third-Harmonic Generation Microscopy for Material Characterization

  • Royon, Arnaud;Bousquet, Bruno;Canioni, Lionel;Treguer, Mona;Cardinal, Thierry;Fargin, Evelyne;Kim, Dae-Geun;Park, Seung-Han
    • Journal of the Optical Society of Korea
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    • 제10권4호
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    • pp.188-195
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    • 2006
  • Third harmonic generation microscopy is described in the frame work of the theory of harmonic generation with Gaussian focused beams inside a bulk material as well as at the vicinity of an interface. This model is then applied to characterize different types of materials in terms of electronic third-order susceptibility. Examples of bulk glasses, poled glasses, laser-induced modifications in glasses and nanoparticles in solution are given in order to give a survey of the broad application field of THG microscopy in material characterization.

Endoscopic Precise 3D Surface Profiler Based on Continuously Scanning Structured Illumination Microscopy

  • Park, Hyo Mi;Joo, Ki-Nam
    • Current Optics and Photonics
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    • 제2권2호
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    • pp.172-178
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    • 2018
  • We propose a precise 3D endoscopic technique for medical and industrial applications. As the 3D measuring principle, the continuously scanning structured illumination microscopy (CSSIM), which enables to obtain 3D sectional images by the synchronous axial scanning of the target with the lateral scanning of the sinusoidal pattern, is adopted. In order to reduce the size of the probe end, the illumination and detection paths of light are designed as coaxial and a coherent imaging fiber bundle is used for transferring the illumination pattern to the target and vice versa. We constructed and experimentally verified the proposed system with a gauge block specimen. As the result, it was confirmed that the 3D surface profile was successfully measured with $16.1{\mu}m$ repeatability for a gauge block specimen. In order to improve the contrast of the sinusoidal illumination pattern reflected off on the target, we used polarizing optical components and confirmed that the visibility of the pattern was suitable in CSSIM.