• Applied Microscopy
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• 제51권
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• pp.9.1-9.10
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• 2021

Brain disorders seriously affect life quality. Therefore, non-invasive neuroimaging has received attention to monitoring and early diagnosing neural disorders to prevent their progress to a severe level. This short review briefly describes the current MRI and PET/CT techniques developed for non-invasive neuroimaging and the future direction of optical imaging techniques to achieve higher resolution and specificity using the second near-infrared (NIR-II) region of wavelength with organic molecules.

• Current Optics and Photonics
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• 제7권5호
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• pp.537-544
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• 2023

Differential phase contrast (DPC) microscopy, a central quantitative phase imaging (QPI) technique in cell biology, facilitates label-free, real-time monitoring of intrinsic optical phase variations in biological samples. The existing DPC imaging theory, while important for QPI, is grounded in paraxial diffraction theory. However, this theory lacks accuracy when applied to high numerical aperture (NA) systems that are vital for high-resolution cellular studies. To tackle this limitation, we have, for the first time, formulated a nonparaxial DPC imaging equation with a transmission cross-coefficient (TCC) for high NA DPC microscopy. Our theoretical framework incorporates the apodization of the high NA objective lens, nonparaxial light propagation, and the angular distribution of source intensity or detector sensitivity. Thus, our TCC model deviates significantly from traditional paraxial TCCs, influenced by both NA and the angular variation of illumination or detection. Our nonparaxial imaging theory could enhance phase retrieval accuracy in QPI based on high NA DPC imaging.

• 한국광학회지
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• 제35권1호
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• pp.9-17
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• 2024

현미경용 플렌옵틱 광학 시스템은 일반적으로 대물 렌즈, 튜브 렌즈, 마이크로 렌즈 어레이, 그리고 이미지 센서로 구성된다. 플렌옵틱을 통한 라이트 필드 이미징에서 튜브 렌즈와 마이크로 렌즈 어레이 간의 수치 구경을 일치시키고, 이를 바탕으로 공간분해능 및 피사계심도 등의 성능 지표를 예측한다. 하지만 상업적 마이크로 렌즈 어레이 적용시 이러한 수치 구경 일치에 어려움이 있어, 본 논문에서는 기존에 보고된 성능 예측 수식을 수치 구경이 일치하지 않는 경우까지 확장하고, 전산 시뮬레이션을 통한 성능 예측 기법을 제시하며, 이를 수치 구경 일치화가 이루어진 10배율 및 수치 구경 불일치가 발생한 20배율 대물렌즈가 적용된 두 개의 플렌옵틱 광학계 개발 및 실험을 통하여 검증하였다. 10배율 및 20배율 시스템은 확장식에서 각각 12.5 ㎛, 6.2 ㎛의 공간 분해능과 530 ㎛, 88 ㎛의 피사계심도를 가지며, 시뮬레이션에서는 각각 11.5 ㎛, 5.8 ㎛의 공간분해능과 510 ㎛, 70 ㎛의 피사계심도를, 실험에서는 각각 11.1 ㎛, 5.8 ㎛의 공간 분해능과 470 ㎛, 70 ㎛의 피사계심도를 가진다. 확장식 및 시뮬레이션 모두 실험 값과 유사한 결과를 보여 시스템 설계에서는 두 가지 방법 모두 적절할 것으로 판단된다. 다만 피사계심도 예측 정확성에 있어서는 시뮬레이션에 의한 예측이 실험 값과 좀 더 유사하므로, 실제 제작에 앞서 시뮬레이션에 의한 성능 예측을 추천한다.

• Journal of the Optical Society of Korea
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• 제16권1호
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• pp.42-46
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• 2012

We have developed a fluorescence optical detection system using a digital micromirror device (DMD) for monitoring 3D cell culture matrices in situ. Full 3D imaging with fast scanning speed was implemented by the combined action of a DMD and a motorized stage. Imaging results with fluorescent microbeads measure the minimum axial resolution of the system as $6.3{\mu}m$, while full 1-mm scanning through 3D alginate-based matrix was demonstrated. For cell imaging, improved images were obtained by removing background fluorescence although the scanning distance was reduced because of low intracellular fluorescence efficiency. The system is expected to be useful to study various dynamics and behaviors of 3-dimensionally cultured cells in microfluidic systems.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2001년도 ICCAS
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• pp.99.3-99
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• 2001

In fluorescence mode confocal scanning microscope, level of detected signal is very low. In object scanning type confocal scanning microscope, the additional optical system with objective lens and plane mirror was proposed to increase signal intensity, but there was none for beam scanning type confocal scanning microscope. We propose reflecting optical systems which improve signal intensity in beam scanning type confocal scanning microscope. We choose one of the proposed optical systems and design the optical system, i.e., select optical components and assign distances between the selected components. To design the optical system, we use finite ray tracing method and make cost function to be minimized.

• 동력기계공학회지
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• 제19권3호
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• pp.5-10
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• 2015

Femtosecond laser machining has been applied for creating a sensor function in silica glass optical fibers. Femtosecond laser pulses make it possible to fabricate micro structures in processed regions of a very thin glass fiber line because femtosecond laser pulses can extremely minimize thermal effects. With the laser machining to optical fiber using a single shot of 210-fs laser at a wavelength of 800 nm, it was observed that a processed region surrounded a thin layer which seemed to be a hollow cavity monitored by scanning electron microscopy (SEM). This study aims at a theoretical investigation for the processed region by using a numerical analysis in order to embed sensing function to optical fibers. Numerical methods based finite element method (FEM) has been used for an optical waveguide modeling. This report suggests two types modeling and describes a comparative study on optical losses obtained by the experiment and the numerical analysis.

• 한국광학회지
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• 제22권1호
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• pp.10-15
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• 2011

본 논문에서 홀로그램의 일부 영역만을 선택하여 자동 초점 맺는 방법을 제안하고 이를 실험적으로 보였다. 먼저 광 스캐닝 홀로그래피를 이용하여 복소 홀로그램을 추출한다. 그 다음 홀로그램의 일부 영역만을 선택하고 가우시안 저대역 필터링, Real-only 홀로그램 합성, Power fringe-adjusted 필터링, 주파수축 변환 등의 과정을 통해 깊이 정보를 추출한다. 이렇게 추출된 깊이 위치를 이용하여 홀로그램을 자동적으로 복원하게 된다.

• 한국전자현미경학회:학술대회논문집
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• 한국현미경학회 2005년도 제36차 추계학술대회 및 제4회 HVEM 이용자 워크샵
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• pp.103-105
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• 2005

• 한국광과학회:학술대회논문집
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• 한국광과학회 2002년도 정기총회 및 제9회 광과학 학술심포지움 논문초록
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• pp.34-34
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• 2002

• 한국생산제조학회지
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• 제8권2호
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• pp.100-107
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• 1999

End milling experiments are conducted to investigate characteristics of laser beam signals due to tool fracture. The laser beam signals are obtained with adapt focusing of tool. Tool states are identified wit h scanning electron microscopy and optical microscopy. It is demonstrated that the laser beam signals provide reliable informations about the cutting processes and tool states. Moreover, tool fracture can be detected successfully using coefficient of variation.