• The Plant Pathology Journal
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• 제36권2호
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• pp.148-156
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• 2020

Pseudomonas syringae pv. tabaci 6605 has two multidrug resistance (MDR) efflux pump transporters, MexAB-OprM and MexEF-OprN. To understand the role of these MDR efflux pumps in virulence, we generated deletion mutants, ΔmexB, ΔmexF, and ΔmexBΔmexF, and investigated their sensitivity to plant-derived antimicrobial compounds, antibiotics, and virulence. Growth inhibition assays with KB soft agar plate showed that growth of the wild-type (WT) was inhibited by 5 μl of 1 M catechol and 1 M coumarin but not by other plant-derived potential antimicrobial compounds tested including phytoalexins. The sensitivity to these compounds tended to increase in ΔmexB and ΔmexBΔmexF mutants. The ΔmexBΔmexF mutant was also sensitive to 2 M acetovanillone. The mexAB-oprM was constitutively expressed, and activated in the ΔmexF and ΔmexBΔmexF mutant strains. The swarming and swimming motilities were impaired in ΔmexF and ΔmexBΔmexF mutants. The flood inoculation test indicated that bacterial populations in all mutant strains were significantly lower than that of WT, although all mutants and WT caused similar disease symptoms. These results indicate that MexAB-OprM extrudes plant-derived catechol, acetovanillone, or coumarin, and contributes to bacterial virulence. Furthermore, MexAB-OprM and MexEF-OprN complemented each other's functions to some extent.

• Journal of Microbiology and Biotechnology
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• 제30권2호
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• pp.244-247
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• 2020

We have expressed extracellular poly(3-hydroxybutyrate) (PHB) depolymerase of Ralstonia pickettii T1 on the Escherichia coli surface using Pseudomonas OprF protein as a fusion partner by C-terminal deletion-fusion strategy. Surface display of depolymerase was confirmed by flow cytometry, immunofluorescence microscopy and whole cell hydrolase activity. For the application, depolymerase was used as an immobilized catalyst of enantioselective hydrolysis reaction for the first time. After 48 h, (R)-methyl mandelate was completely hydrolyzed, and (S)-mandelic acid was produced with over 99% enantiomeric excess. Our findings suggest that surface displayed depolymerase on E. coli can be used as an enantioselective biocatalyst.

• 한국문헌정보학회지
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• 제57권2호
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• pp.79-96
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• 2023

본 연구는 대표적인 OPR(Open Peer Review) 플랫폼인 F1000Research에서 사회과학분야의 논문 585건을 대상으로 개방형 동료 심사 결과를 분석하고 피인용, 알트메트릭스와 어떠한 관련성을 보이는지 확인하였다. 더불어 논문의 활용이 피인용에 미치는 영향 관계 내에서 심사 점수가 조절효과(Moderating effect)를 나타내는지 검증함으로써, OPR 환경에서 고품질로 평가된 논문이 피인용을 촉진할 수 있는지 확인하였다. 분석 결과 첫 번째, 승인과 조건부 승인된 논문 그룹 간에 피인용 횟수에 유의미한 차이가 나타나지 않았지만, 환산된 심사 점수와 피인용 횟수는 유의한 정(+)의 상관성(r= 0.40 ~ 0.60)을 나타냈다. 두번째, 심사 점수는 알트메트릭스와도 약한 상관성을 나타내 심사자의 품질 평가 결과는 피인용과 사회적 영향을 약하게 예측할 수 있는 것으로 분석되었다. 마지막으로 심사 점수는 논문의 활용을 피인용으로 이어지게 하는데 유의한 양의 방향의 조절효과 (B=1.69, P < 0.01)를 수행하며, 조건부 효과 검사 결과 가장 고품질로 평가된 논문 집단에서 가장 큰 효과(B=11.32, 95% CI [10.57, 12.08])를 나타내는 것으로 검증되었다. 따라서 공개된 심사 결과는 연구자들의 우수 논문 선별을 도와 인용을 유도하는데 도움을 줄 수 있는 것으로 파악되었다.

• Ocean and Polar Research
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• 제38권1호
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• pp.59-70
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• 2016

Establishing the strategic plans to foster the oceans and fisheries (O&F) industry as an engine for national sustainable economic growth has become an important task for developing countries as well as developed countries. The first step to do so is to identify O&F industry and analyze its economic effects. Therefore, the prime purposes of the paper are two-fold. The first is to identify O&F industry and estimate its market size using 2012 Input-Output (I-O) table published by the Bank of Korea. The second purpose is to obtain some quantitative information on production-inducing effect, value-added creation effect, and employment-inducing effect of the O&F industry. To this end, we apply an IO analysis using exogenous specification of the O&F industry. The results show that the O&F industry covers 4.1% and 3.0% of national output and gross domestic product, respectively. Moreover, we found that 1.0 won of production or investment in the O&F industry induces 1.7363 won of production and 0.4759 won of value-added in the national economy. One billion won of production or investment in the O&F industry touches off 7.5569 persons of employment. This information can be utilized in the O&F industry-related policy-making.

• Ocean and Polar Research
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• 제30권3호
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• pp.335-345
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• 2008

In order to study spatial variabilities and major controlling factors, we measured fugacity of $CO_2(fCO_2)$, temperature, salinity and nutrients in surface waters of the North Pacific($7^{\circ}30'{\sim}33^{\circ}15'N$, $123^{\circ}56'E{\sim}164^{\circ}24'W$) between September$\sim$October 2007. The North Pacific and the marginal sea were distinguished by $fCO_2$ distribution as well as unique characteristics of temperature and salinity. There was a distinct diurnal SST variation in the tropical North Pacific area, and surface $fCO_2$ coincidently showed diurnal variation. In the North Pacific area, surface $fCO_2$ was mainly controlled by temperature, while in the marginal sea area it was primarily dependent on alkalinity and dissolved inorganic carbon concentrations. Air-sea $CO_2$ flux showed a large spatial variation, with a range of $-6.10{\sim}5.06\;mmol\;m^{-2}day^{-1}$. The center of subtropical gyre of North Pacific acted as a source of $CO_2(3.09{\pm}0.95\;mmol\;m^{-2}day^{-1})$. Tropical western North Pacific (i.e. the 'warm pool' area and the subtropical western North Pacific) acted as weak sources of $CO_2$($1.07{\pm}1.20\;mmol\;m^{-2}day^{-1}$ and $0.50{\pm}0.53\;mmol\;m^{-2}day^{-1}$, respectively). In the marginal sea, however, the flux was estimated to be $-0.68{\pm}1.17\;mmol\;m^{-2}day^{-1}$, indicating that this area acted as a sink for $CO_2$.

• Ocean and Polar Research
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• 제28권4호
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• pp.395-405
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• 2006

We measured the fugacity of $CO_2$ $(fCO_2)$, temperature, salinity, nutrients and chlorophyll a in the surface water of the western North Pacific $(4^{\circ}30'{\sim}33^{\circ}10'N,\;144^{\circ}20'{\sim}127^{\circ}35'E)$ in September 2002. There were zonally several major currents which have characteristics of specific temperature and salinity (NECC, North Equatorial Counter Current; NEC, North Equatorial Current; Kuroshio etc.). Surface $fCO_2$ distribution was clearly distinguished into two groups, tropical and subtropical areas of which boundary was $20^{\circ}N$. In the tropical Int surface $fCO_2$ was mainly controlled by temperature, while in the subtropical area, surface $fCO_2$ was dependent on total inorganic carbon contents. Air-sea $CO_2$ flux showed a large spatial variation, with a range of $-0.69{\sim}0.79 mmole\;m^{-2}day^{-1}$. In the area of AE (Anticyclonic Eddy), SM(Southern Mixed region) and NM (Northern Mixed region), the ocean acted as a weak source of $CO_2$ $(0.6{\sim}0.79 mmole\; m^{-2}day^{-1})$. In NECC, NEC, Kuroshio and ECS (East China Sea), however, the fluxes were estimated to be $-0.3mmole\; m^{-2}day^{-1})$ for the first three regions and $-1.2mmole\; m^{-2}day^{-1})$ for ECS respectively, indicating that these areas acted as sinks of $CO_2$. The average air-sea flux in the entire study area was $0.15mmole\;m^{-2}day^{-1})$, implying that the western North Pacific was a weak source of $CO_2$ during the study period.

• Ocean and Polar Research
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• 제40권1호
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• pp.23-35
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• 2018

A small cyclopoid copepod M. subtilis (Giesbrecht, 1893 ["1892"]) belonging to the genus Monothula $B{\ddot{o}}ttger-Schnack$ and Huys, 2001 was collected by using $60{\mu}m$ mesh net and firstly recorded in the epipelagic layer of the equatorial Pacific Ocean. We redescribed its morphological characteristics for both female and male, comparing with those of previous studies. Specimens of M. subtilis from the equatorial Pacific Ocean differ from those previously reported by others in terms of the length of the seta G on antenna, being much shorter than setae E and F; in the distal spine on the swimming leg 4, being longer than the length of the third segment on P4. The outer spine of the P3 enp-3 in male is slightly over the tip of conical process. The spine lengths of the distal endopods of P2-P4 for both sexes showed variations among individuals, and the proportions of spine lengths in female are higher than those in male.

• Ocean and Polar Research
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• 제32권4호
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• pp.369-377
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• 2010

To characterize ecotoxicological responses to a natural estrogen, $17{\beta}$-estradiol, we evaluated the life-history of the parental ($F_0$) and first generation ($F_1$) of the harpacticoid copepod, Tigriopus japonicus sensu lato. We evaluated the survival of nauplii and copepodites, the number of days until the emergence of copepodites and adult males, the sex ratio, brooding success, and the first brooding day of adult females. No significant differences in the survival rate were noted in response to treatments with different concentrations of $17{\beta}$-estradiol. However, $17{\beta}$-estradiol induced developmental delay and skewed the sex ratio toward males. Copepod development was delayed significantly in the 0.1 and $1\;{\mu}g\;l^{-1}$ $17{\beta}$-estradiol treatment groups relative to the control group, with a more pronounced delay in the $F_1$ group. Body length and biomass were significantly smaller in the $17{\beta}$-estradiol treated groups than in the controls. The male emergence of T. japonicus s.l. was very high in the 10 and $30\;{\mu}g\;l^{-1}$ $17{\beta}$-estradiol treatment group. Furthermore, exposure to $17{\beta}$-estradiol resulted in morphological deformities such as shrinking and swelling of the urosome, twisted setae of the caudal rami, setal loss of swimming legs, abnormal segmentation of antennules, and dwarfism.

• Ocean and Polar Research
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• 제24권3호
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• pp.263-266
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• 2002

Gravity waves have been searched for with a new all-sky camera system over Korean Peninsular. The all-sky camera consists of a 37mm/F4.5 Mamiya fisheye lens with a 180 dog field of view, interference filters and a 1024 by 1024 CCD camera. The all-sky camera has been tested near Daejeon city, and moved to Mt. Bohyun where the largest astronomical telescope is operated in Korea. A clear wave pattern was successfully detected in OH filter images over Mt. Bohyun on July 18, 2001, indicating that small scale coherent gravity waves perturbed OH airglow near the mesopause. Other wave features are since then observed with Na 589.8nm and OI 630.0nm filters. Since a Japanese all-sky camera network has already detected traveling ionospheric disturbances (TID) over the northeast-southwest range of Japanese islands, we hope our all-sky camera extends the coverage of the TID's observations to the west direction. We plan to operate our all-sky camera all year around to study seasonal variation of wave activities over the mid-latitude upper atmosphere.

• 한국압력기기공학회 논문집
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• 제7권3호
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• pp.61-66
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• 2011

The integrity of the SG(Steam Generator) tubes has been challenged by numerous factors such as flaws, operation, atmosphere, inherently degraded materials, loose parts and even human errors. Of the factors, loose parts(or foreign materials) on the secondary side of the tubes can bring about volumetric defects and even leakage from the primary to the secondary side in a short period of time. More serious concerns about the loose parts are their unknown influx path and rapid growth rate of the defects affected by the loose parts. Therefore it is imperative to detect and characterize the foreign materials and the defects. As a part of the measures for loose part detection, TTS(Top of Tubesheet) MRPC(Motorized Rotating Pancake Coils) ECT has been carried out especially to the restricted high probability area of the loose part. However, in the presence of loose parts in the other areas, wide range loose part detection techniques are required. In this study, loose part standard tube was presented as a way to accurately detect and characterize loose part signals. And the SG tube ECT bobbin coil and MRPC ISI(In-service Inspection) data of domestic OPR-1000 and Westinghouse Model F(W_F) were reviewed and consequently, comprehensive loose part detection technique is derived especially by applying bobbin coil signals