• Title/Summary/Keyword: Operation Strategies

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Development of Nested PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Cylindrocladium scoparium on Eucalyptus

  • Qiao, Tian-Min;Zhang, Jing;Li, Shu-Jiang;Han, Shan;Zhu, Tian-Hui
    • The Plant Pathology Journal
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    • v.32 no.5
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    • pp.414-422
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    • 2016
  • Eucalyptus dieback disease, caused by Cylindrocladium scoparium, has occurred in last few years in large Eucalyptus planting areas in China and other countries. Rapid, simple, and reliable diagnostic techniques are desired for the early detection of Eucalyptus dieback of C. scoparium prior to formulation of efficient control plan. For this purpose, three PCR-based methods of nested PCR, multiplex PCR, loop-mediated isothermal amplification (LAMP) were developed for detection of C. scoparium based on factor 1-alpha (tef1) and beta-tubulin gene in this study. All of the three methods showed highly specific to C. scoparium. The sensitivities of the nested PCR and LAMP were much higher than the multiplex PCR. The sensitivity of multiplex PCR was also higher than regular PCR. C. scoparium could be detected within 60 min from infected Eucalyptus plants by LAMP, while at least 2 h was needed by the rest two methods. Using different Eucalyptus tissues as samples for C. scoparium detection, all of the three PCR-based methods showed much better detection results than regular PCR. Base on the results from this study, we concluded that any of the three PCR-based methods could be used as diagnostic technology for the development of efficient strategies of Eucalyptus dieback disease control. Particularly, LAMP was the most practical method in field application because of its one-step and rapid reaction, simple operation, single-tube utilization, and simple visualization of amplification products.

Traumatic Pseudoaneurysm of the Superficial Temporal Artery diagnosed with 3-Dimensional Computed Tomography Angiography: Two Cases Report (3차원 컴퓨터단층촬영 혈관조영술을 이용하여 진단한 외상성 천측두동맥 가성동맥류의 치험례)

  • Kwon, Ho;Hwang, Dong Yeon;Jung, Sung-No;Yim, Young Min;Shin, Ok Ran
    • Archives of Plastic Surgery
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    • v.34 no.2
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    • pp.265-268
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    • 2007
  • Purpose: Superficial temporal artery(STA) aneurysms are very rare and mostly occur as pseudoaneurysms secondary to trauma. Clinical diagnosis of STA pseudoaneurysm is based on a history of trauma or surgery to frontotemporal region, which presents with pulsatile mass. To confirm diagnosis, many imaging strategies can be used such as digital subtraction angiography, sonography, CT and MRI. But, these imaging modalities are invasive or inaccurate or expensive. Thus, we used 3D CT angiography to confirm STA aneurysm and to get accurate information. Methods: We have experienced two cases of pulsatile mass on the temporal area, suspected as STA pseudoaneurysms. On the basis of clinical information, we performed 3D CT angiography to get more accurate information about this pulsatile mass and to confirm diagnosis. On the basis of information from 3D CT angiography, we performed operation. Results: The lesions were diagnosed as pseudoaneurysm of superficial temporal artery by 3D CT angiography, and surgically resected safely without any complication on the basis of information from 3D CT angiography. 3D CT angiography was excellent diagnostic method for detecting STA pseudoaneurysms, and effectively showed many information about pseudoanerysms such as relationship between the aneurysms and surrounding structures, and its size. Conclusion: We could effectively diagnose and treat on the basis of information from 3D CT angiography. We present our cases with a brief review of the literature related to STA traumatic pseudoaneurysms.

Development of Water Supply Capacity Index to Monitor Droughts in a Reservoir (저수지 가뭄감시를 위한 물공급능력지수의 개발)

  • Lee, Dong-Ryul;Moon, Jang-Won;Lee, Dae-Hee;Ahn, Jae-Hyun
    • Journal of Korea Water Resources Association
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    • v.39 no.3 s.164
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    • pp.199-214
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    • 2006
  • The efficient management strategies of reservoirs in periods of drought event are an essential element for drought planning. A Water Supply Capacity Index (WSCI) has been developed for the evaluation and effective monitoring of the supply capacity of a reservoir during a drought event. The WSCI is a measure of the duration that a reservoir can supply a required demand under the worst drought condition. The WSCI is not only a useful standard to refer to when making decisions on reservoir operations in a period of drought, but it can be also applied for setting the drought trigger in water demand sites supplied from the reservoir. The correlation between the standardized WSCI and existing drought indices such as PDSI, SPI and SWSI has been analyzed to the applicability of WSCI.

Assessment of Selective Withdrawal Facility in the Imha Reservoir Using CE-QUAL-W2 Model (CE-QUAL-W2 모델을 이용한 임하호 선택배제시설의 효과분석)

  • Lee, Sanguk;Kim, Jeongkon;Noh, Joonwoo;Ko, Ick Hwan
    • Journal of Korean Society on Water Environment
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    • v.23 no.2
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    • pp.228-235
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    • 2007
  • For efficient turbid water withdrawal in the Imha reservoir, a selective withdrawal facility was recently installed and operated during summer season of year 2006. In this research, CE-QUAL-W2 model was utilized to assess the efficiency of the selective withdrawal facility, in comparison with the original surface withdrawal, on turbid water management. Model calibration was carried out using data observed at four automatic monitoring stations in the reservoir. It was found that the model appropriately simulated, with the RMSE less than 5.2 NTU, the observed vertical and horizontal distributions of water temperature and turbidity as well as the location of maximum turbid water at each monitoring station. The analysis results showed that selective withdrawal is more effective in removing high turbid water than surface withdrawal as selective withdrawal contributed to reducing $35Mm^3$ of high turbidity water (> 100 NTU) in the reservoir by increasing outflows of high turbid water. Therefore, effective management of turbid water in the reservoir can be achieved by changing locations of intake depending on turbid water distribution conditions. The results of this study will provide some basic information for establishing better operation strategies to cope with turbid water problems.

Understanding Three-dimensional Printing Technology, Evaluation, and Control of Hazardous Exposure Agents (3D 프린팅 기술의 이해, 유해 인자 노출 평가와 제어)

  • Park, Jihoon;Jeon, Haejoon;Oh, Youngseok;Park, Kyungho;Yoon, Chungsik
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.28 no.3
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    • pp.241-256
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    • 2018
  • Objectives: This study aimed to review the characteristics of three-dimensional printing technology focusing on printing types, materials, and health hazards. We discussed the methodologies for exposure assessment on hazardous substances emitted from 3D printing through article reviews. Methods: Previous researches on 3D printing technology and exposure assessment were collected through a literature review of public reports and research articles reported up to July 2018. We mainly focused on introducing the technologies, printing materials, hazardous emissions during 3D printing, and the methodologies for evaluation. Results: 3D printing technologies can be categorized by laminating type. Fused deposition modeling(FDM) is the most widely used, and most studies have conducted exposure assessment using this type. The printing materials involved were diverse, including plastic polymer, metal, resin, and more. In the FDM types, the most commonly used material was polymers, such as acrylonitrile-butadiene-styrene(ABS) and polylactic acids(PLA). These materials are operated under high-temperature conditions, so high levels of ultrafine particles(mainly nanoparticle size) and chemical compounds such as organic compounds, aldehydes, and toxic gases were identified as being emitted during 3D printing. Conclusions: Personal desktop 3D printers are widely used and expected to be constantly distributed in the future. In particular, hazardous emissions, including nano sized particles and various thermal byproducts, can be released under operation at high temperatures, so it is important to identify the health effects by emissions from 3D printing. Furthermore, appropriate control strategies should be also considered for 3D printing technology.

Parenteral Nutrition-Associated Cholestasis in Very Low Birth Weight Infants: A Single Center Experience

  • Kim, Ah-Young;Lim, Ryoung-Kyoung;Han, Young-Mi;Park, Kyung-Hee;Byun, Shin-Yun
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.19 no.1
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    • pp.61-70
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    • 2016
  • Purpose: Parenteral nutrition (PN)-associated cholestasis (PNAC) is one of the most common complications in very low birth weight infants (VLBWIs). The aim of this study is to evaluate the risk factors of PNAC in VBLWIs. Methods: We retrospectively reviewed the medical records of 322 VLBWIs admitted to the neonatal intensive care unit of our hospital from July 1, 2009 to December 31, 2013. We excluded 72 dead infants; 6 infants were transferred to another hospital, and 57 infants were transferred to our hospital at 2 weeks after birth. The infants were divided into the cholestasis and the non-cholestasis groups. PNAC was defined as a direct bilirubin level of ${\geq}2.0mg/dL$ in infants administered with PN for ${\geq}2weeks$. Results: A total of 187 VLBWI were enrolled in this study; of these, 46 infants developed PNAC. Multivariate logistic regression analysis showed that the risk factors of PNAC in VLBWI were longer duration of antimicrobial use (odds ratio [OR] 4.49, 95% confidence interval [95% CI] 4.42-4.58), longer duration of PN (OR 2.68, 95% CI 2.41-3.00), long-term lack of enteral nutrition (OR 2.89, 95% CI 2.43-3.37), occurrence of necrotizing enterocolitis (OR 2.40, 95% CI 2.16-2.83), and gastrointestinal operation (OR 2.19, 95% CI 2.03-2.58). Conclusion: The results of this study suggest that shorter PN, aggressive enteral nutrition, and appropriate antimicrobial use are important strategies in preventing PNAC.

Aligning Academic Library Makerspaces with Digital Literacy Education Spaces (디지털리터러시 교육 공간으로서의 대학도서관 메이커스페이스)

  • Chang, Yunkeum
    • Journal of the Korean Society for Library and Information Science
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    • v.52 no.1
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    • pp.425-446
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    • 2018
  • As makerspaces continue to be introduced in academic libraries in Korea, this study explores potential operating strategies of, and long-term justifications for, makerspaces as digital literacy eduational spaces and services at academic libraries. By examining related literature reviews and case studies of makerspaces, this study analyzes various programs and their respective creation, funding, development, and outcomes, including educational value and library-specific goals such as digital literacy and lifelong learning. This study also considers the perspectives of librarians at academic libraries in Korea who were asked about the purpose, impact, and limitations of makerspaces. Certain common themes appear: for example, it is necessary for makerspaces to resolve challenges related to stable funding, as well as staffing and training of professional librarians assisting with the on-the-ground operation of makerspaces. This study proposes that designing makerspaces for an academic library setting goes deeper than providing a collaborative environment with access to new technologies like 3D printers and laser cutters, and it may be uniquely appropriate to draw connections to libraries' objectives to provide digital literacy education and universities' mission to foster innovation and creativity among students.

Scenarios for Effective Fire Fighting Operations during Tunnel Fires (도로터널 화재시 효과적인 소방활동전략 수립을 위한 시나리오 연구)

  • Kim, Hak kuen;Lee, Ji-hee
    • Fire Science and Engineering
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    • v.31 no.5
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    • pp.107-116
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    • 2017
  • Fires in tunnels are an international concern and fatal accidental fires in tunnels seem to occur on annual. They have the potential to become much worse int the future as more and longer tunnels are constructed and as traffic densities increase. This is a serious problem. The main purpose of this study is to develop operational procedures for fire brigades in road tunnel fires. This study discussed the past to see what can be learned from the incidents that have already done in tunnels. 73 cases of road tunnel fires domestic and outside of Korea were investigated and classified into 4 incident categories. Among them, 4 tunnel fires are highlighted, focusing on the activities of fire brigades and operation. Regarding the establishment of the strategies for fire fighting, 6 kinds of fire scenario curves have been deducted with regard to the relation between intervention time and heat release rate. It made the choice from the defensive or aggressive fire fighting activities depending on two criteria i.e. response limit and maximum response time. Road Tunnel Classification models can be useful when a fire brigade evaluates fire risk levels in the tunnels under its jurisdiction from the firefighting point of view and sets up preventive measures.

Fuzzy LP Based Power Network Peak Shaving Algorithm (퍼지 LP 기반 전력망 Peak Shaving 알고리즘)

  • Ohn, Sungmin;Kim, Jung-Su;Song, Hwachang;Chang, Byunghoon
    • Journal of the Korean Institute of Intelligent Systems
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    • v.22 no.6
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    • pp.754-760
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    • 2012
  • This paper describes peak shaving algorithms as long-term cycle scheduling in the power management system (PMS) for MW-scale battery energy storage systems (BESS). The purpose of PMS is basically to manage the input and output power from battery modules placed in the systems. Assuming that an one-day ahead load curve is provided, off-line peak shaving algorithms can be employed, but applying the results of the off-line algorithm may result in the difference in the real-time performance because there is uncertainty in the provided load curve. This paper adopts fuzzy based LP (linear programming) algorithms for describing the peak shaving algorithm in PMS and discusses a solution technique and real-time operation strategies using the solution.

Estimation of greenhouse gas (GHG) emission from wastewater treatment plants and effect of biogas reuse on GHG mitigation

  • Chang, Jin;Kyung, Daeseung;Lee, Woojin
    • Advances in environmental research
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    • v.3 no.2
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    • pp.173-183
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    • 2014
  • A comprehensive mathematical model was developed for this study to estimate on-site and off-site GHG emissions from wastewater treatment plants (WWTPs). The model was applied to three different hybrid WWTPs (S-WWTP, J-WWTP, and T-WWTP) including anaerobic, anoxic, and aerobic process, located in Seoul City, South Korea. Overall on-site and off-site GHG emissions from S-WWTP, J-WWTP, and T-WWTP were $305,253kgCO_2e/d$, $282,682kgCO_2e/d$, and $117,942kgCO_2e/d$, respectively. WWTP treating higher amounts of wastewater produced more on-site and off-site GHG emissions. On average, the percentage contribution of on-site and off-site emissions was 3.03% and 96.97%. The highest amount of on-site GHG emissions was generated from anoxic process and the primary on-site GHG was nitrous oxide ($N_2O$). Off-site GHG emissions related to electricity consumption for unit operation was much higher than that related to production of chemicals for on-site usage. Recovery and reuse of biogas significantly reduced the total GHG emissions from WWTPs. The results obtained from this study can provide basic knowledge to understand the source and amount of GHG emissions from WWTPs and strategies to establish lower GHG emitting WWTPs.