• Animal cells and systems
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• 제3권3호
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• pp.319-329
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• 1999

Onset of female puberty follows a series of prepubertal cellular and molecular events including changes of synaptic plasticity, synthetic and releasing activity and gene expression. Dramatic increase of gonadal steroid level is one of the most prominent changes before the onset of puberty. Based on the importance of steroid feedback upon the hypothalamus, we adopted an estrogen sterilized rat (ESR) model where 100 ng of 17$\eta$-estradiol were administered into neonatal pubs for 7 days after birth. To identify genes involved in the onset of female puberty, we applied PCR differential display using RNA samples derived from ESR and control rat hypothalami. About 100 out of more than 1000 RNA species examined displayed differential expression patterns between a 60-day old control rat and ESR. Sequence analysis of differentially amplified PCR products showed homology with genes such as mouse kinesin superfamily-associated protein 3 (KAP3) and several cDNAs previously described by others in mouse and human tissues. Several gene products such as 2-1 and 8-1 corresponded to novel DNA sequences. We analyzed mRNA levels of KAP3, 2-1 and 8-1 genes in the hypothalami derived from neonatal, 6-, 28-, 31-, and 40-day old rats. Northern blot analysis showed that mRNAs of KAP3, 2-1 and 8-1 genes were markedly increased before the initiation of puberty. Neonatal treatment of estrogen clearly inhibited prepubertal increases in KAP3, 2-1 and 8-1 mRNA levels. Therefore, these genes may play important roles in the initiation of hypothalamic puberty. In addition, intracerebroventricular (icv) injection of antisense KAP3 oligodeoxynucleotide (ODN) clearly delayed puberty initiation determined by vaginal opening, which further confirmed that KAP3 plays an important role in the regulation of puberty initiation.

• Journal of Oral Medicine and Pain
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• 제14권1호
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• pp.57-66
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• 1989

66 patients with temporomandibular disorders were selected for experimental group, and 45 normal subjects who were Dental students were selected for control group. Average age of experimental group was 30.5 years, Male to Female ratio was 2 : 3, and their age distribution were teen-ages to seventh decades. Transcranial radiography (TR) with Denar Accurad 100 was used for each group to get the values of width in joint spaces and to investigate the bony changes of articular surfaces and relative condylar position to articular fossa. In addition to TR, clinical interview and routine charting about amount of mandibular movements and occlusal variations were carried out in experimental group. The obtained results were as follows : 1. The mean values of joint space with in control group were 2.15mm to anterior, 2.98mm to superior and 2.29mm to posterior and the value of relative condylar position to the deepest portion of articular fossa was 0.21mm to anterior. In experimental group, those values were 2.01mm, 2.14mm 2.22mm and 0.12mm to posterior in sequence, respectively. Joint spaces in experimental group, therefore, were inclined to decreased, and relative condylar position was inclined to retrude. Joint space in control group showed symmetric condylar position, but in experimental group showed asymmetry. 2. Non-affected joints with no bony changes in experimental group showed the narrowest joint spaces which were thought to manifest the abnormal stress to non-affected side to dysfunctional state of contralateral affected joints. 3. Amount of mandibular movements in experimental group were within normal values in lateral movements and in protrusive movement but in opening movement with or without passive stretch, those were lower than normal values. Frequency of occlusal variation, for example, protrusive posterior contacts, open bite, median line shift to lateral side were inclined to increase with bony changes and with crepitus.

• Parasites, Hosts and Diseases
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• 제54권6호
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• pp.777-786
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• 2016

Raillietina species are prevalent in domestic chickens (Gallus gallus domesticus) in Phayao province, northern Thailand. Their infection may cause disease and death, which affects the public health and economic situation in chicken farms. The identification of Raillietina has been based on morphology and molecular analysis. In this study, morphological observations using light (LM) and scanning electron microscopies (SEM) coupled with molecular analysis of the internal transcribed spacer 2 (ITS2) region and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 (ND1) gene were employed for precise identification and phylogenetic relationship studies of Raillietina spp. Four Raillietina species, including R. echinobothrida, R. tetragona, R. cesticillus, and Raillietina sp., were recovered in domestic chickens from 4 districts in Phayao province, Thailand. LM and SEM observations revealed differences in the morphology of the scolex, position of the genital pore, number of eggs per egg capsule, and rostellar opening surface structures in all 4 species. Phylogenetic relationships were found among the phylogenetic trees obtained by the maximum likelihood and distance-based neighbor-joining methods. ITS2 and ND1 sequence data recorded from Raillietina sp. appeared to be monophyletic. The query sequences of R. echinobothrida, R. tetragona, R. cesticillus, and Raillietina sp. were separated according to the different morphological characters. This study confirmed that morphological studies combined with molecular analyses can differentiate related species within the genus Raillietina in Thailand.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2006년도 춘계학술대회 논문집
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• pp.55-56
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• 2006

This paper describes a new concept of the robot that can climb on the vertical plane. The engineering design problem of the main structure is presented and the experimental results regarding a new mechanism of climbing on the vertical wall are discussed. The locomotive motion of the robot is realized by using a series chain of two caterpillar wheels on which 24-suction pads are installed. White each caterpillar wheel rotates on the vertical plane surface, the vacuum pads are activated in sequence based on the sequential opening by specially designed mechanical valves. The detail design feature of the valve is also described in this paper. The overall size of the robot is around 460 mm in width and length, respectively, and 200 mm in height. Its mass is slightly over 14 kg. The main mechanical structure of the robot consists of driving motors, vacuum caterpillar system, steering part, vacuum pump and battery. The performance of the robot is verified on the vertical wall.

• 대한기계학회논문집A
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• 제35권11호
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• pp.1483-1490
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• 2011

전동기 일체형 유압액추에이터(EHA)는 전기모터와 직결되어 구동되는 유압펌프를 이용하여 유압 피스톤의 변위나 속도를 제어할 수 있도록 모듈화된 유압기기로 효율이 높고 독립된 동력원을 갖는 장점으로 인하여 항공기의 전자구동시스템에 적용되어 왔다. 최근에는 다양한 형태의 유압시스템이 적용되고 있는 건설중장비 분야에서 엔진과 전기모터를 결합한 하이브리드 기술이 개발됨에 따라 EHA의 활용범위가 확대될 것으로 예상된다. 본 연구에서는 편로드 유압실린더를 갖는 EHA가 적용된 유압시스템을 대상으로 펌프작동에 따른 유압실린더의 기동 및 정지특성을 고찰하기 위하여 EHA 내부 유압회로의 기능을 파악하고 동특성 모델을 유도하였으며 파일럿작동 체크밸브의 크래킹압력을 분석하여 개방 및 폐쇄특성을 예측하였으며 시뮬레이션을 통하여 분석된 결과를 검증하였다.

• The Plant Pathology Journal
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• 제35권2호
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• pp.178-187
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• 2019

Plants are exposed to biotic stresses caused by pathogen attack and complex abiotic stresses including heat and drought by dynamic climate changes. To alleviate these stresses, we investigated two bacterial stains, H26-2 and H30-3 in two cultivars ('Ryeokkwang' and 'Buram-3-ho') of Chinese cabbage in plastic pots in a greenhouse. We evaluated effects of bacterial strains on plant growth-promotion and mitigation of heat and drought stresses; the role of exopolysaccharides as one of bacterial determinants on alleviating stresses; biocontrol activity against soft rot caused by Pectobacterium carotovorum subsp. carotovorum PCC21. Strains H26-2 and H30-3 significantly increased fresh weights compared to a $MgSO_4$ solution; reduced leaf wilting and promoted recovery after re-watering under heat and drought stresses. Chinese cabbages treated with H26-2 and H30-3 increased leaf abscisic acid (ABA) content and reduced stomatal opening after stresses treatments, in addition, these strains stably colonized and maintained their populations in rhizosphere during heat and drought stresses. As well as tested bacterial cells, exopolysaccharides (EPS) of H30-3 could be one of bacterial determinants for alleviation of tested stresses in Chinese cabbages, however, the effects were different to cultivars of Chinese cabbages. In addition to bacterial activity to abiotic stresses, H30-3 could suppress incidence (%) of soft rot in 'Buram-3-ho'. The tested strains were identified as Bacillus aryabhattai H26-2 and B. siamensis H30-3 based on 16S rRNA gene sequence analysis. Taken together, H26-2 and H30-3 could be candidates for both plant growth promotion and mitigation of heat and drought stresses in Chinese cabbage.

• 한국추진공학회지
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• 제26권4호
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• pp.64-71
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• 2022

개량된 성능의 상단엔진 개발을 위해 다단연소 사이클 액체로켓엔진의 연구가 진행 중이다. 재점화 기술을 개발하기 위한 수류시험, 점화시험, 연소시험을 계획하여 수행하였다. 재점화 시 터보펌프에서 캐비테이션 현상을 발생시킬 수 있는 연료라인으로의 퍼지가스 유입문제를 해결하기 위해, 각 단계의 시험결과를 분석하였다. 분석결과를 바탕으로 기포제거 밸브의 작동, 퍼지밸브와 엔진 연료밸브의 열림 중첩시간 줄임을 통해 퍼지가스 유입문제를 해결하였다. 이를 바탕으로 재점화 연소시험을 성공적으로 수행하였다.

• Journal of Oral Medicine and Pain
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• 제38권3호
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• pp.275-289
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• 2013

본 연구는 측두하악장애에 대한 치과 내원환자의 인지도와 이해도를 조사, 분석하기 위해 시행되었다. 인제대학교 부산백병원 치과 혹은 울산광역시 소재 한빛치과병원을 방문한 총 243명의 환자에게 설문조사를 시행하였고, 설문지의 모든 문항에 대해 성실히 답한 195명을 대상으로 하여 다음과 같은 결과를 얻었다. 1. "측두하악장애"란 용어를 인지하고 있는 환자는 전체 대상자의17.4%였고, 총 12년 이상의 학력을 가진 군(82.4%, p<0.01)은 다른 군에서 보다 "측두하악장애"에 대한 인지도가 유의하게 높았다. "턱관절질환"이란 용어를 인지하고 있는 환자는 전체 대상자의 81.0%였고, 30-49세 연령군(45.6%, p<0.05)과 총 12년 이상의 학력을 가진 군(60.1%, p<0.01)에서 다른 군보다 "턱관절질환"이란 용어에 대한 인지도가 유의하게 높았다. 2. 전체의 과반수 이상인 50.6%가 턱관절질환의 개념으로 "턱을 무리하게 사용해서 생기는 병이다"를 선택했다. 3. 턱관절질환에 대해 들은 경로로TV, 라디오(41.4%)가 가장 많았고, 가족과 친구(20.2%), 병원 및 병원관계자(18.2%), 인터넷(15.7%) 및 신문과 잡지(4.5%)순이었다. TV, 라디오를 통해서 턱관절질환에 대해 들어본 응답자 중 30-49세 연령군이 다른 군보다 유의하게 높은 비율(52.4%, p<0.05)을 보였다. 인터넷을 통해서 턱관절질환에 대해 들어본 응답자 중 18-29세 연령군이 다른 군보다 유의하게 높은 비율(61.3%, p<0.01)을 보였다. 병원과 병원관계자를 통해서 턱관절질환에 대해 들어본 응답자 중 총 12년 이상의 학력을 가진 군(75%, p<0.05)이 다른 군보다 유의하게 높은 비율을 보였다. 4. 환자들은 입을 벌리고 다물 때 턱에서 나는 소리(26.9%), 입을 크게 벌리지 못한다(25.1%), 귀 앞쪽 부위의 통증(13.7%)을 턱관절질환의 증상과 징후로 골랐다. 턱관절질환의 원인으로는 딱딱하거나 질긴 음식을 즐겨먹기(19.5%), 아래, 윗니가 잘 안 맞물림(19.0%), 한쪽 어금니로 음식씹기(18.5%)를 많이 선택했다. 턱관절질환의 치료법으로 턱관절수술(28%)을 가장 많이 꼽았고, 구강내 장치치료(23.9%), 물리치료(14.6%)가 그 뒤를 따랐다. 턱관절질환의 예방법으로 딱딱한 음식의 섭취를 줄인다(21.1%), 입을 너무 크게 벌리지 않는다(17.0%), 양쪽 어금니로 음식을 씹는다(15.4%)를 많이 선택했다.

• 한국의학물리학회:학술대회논문집
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• 한국의학물리학회 2002년도 Proceedings
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• pp.248-251
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• 2002

The intensity modulated radiation therapy (IMRT) with a multileaf collimator (MLC) requires the conversion of a radiation fluence map into a leaf sequence file that controls the movement of the MLC during radiation treatment of patients. Patient dose verification is clinically one of the most important parts in the treatment delivery of the radiation therapy. The three dimensional (3D) reconstruction of dose distribution delivered to the target helps to verify patient dose and to determine the physical characteristics of beams used in IMRT. A new method is presented for the pretreatment dosimetric verification of two dimensional distributions of photon intensity by means of Beam Intensity Scanner System (BISS) as a radiation detector with a custom-made software for dose calculation of fluorescence signals from scintillator. The scintillator is used to produce fluorescence from the irradiation of 6MV photons on a Varian Clinac 21EX. The BISS reproduces 3D- relative dose distribution from the digitized fluoroscopic signals obtained by digital video camera-based scintillator(DVCS) device in the IMRT. For the intensity modulated beams (IMBs), the calculations of absorbed dose are performed in absolute beam fluence profiles which are used for calculation of the patient dose distribution. The 3D-dose profiles of the IMBs with the BISS were demonstrated by relative measurements of photon beams and shown good agreement with radiographic film. The mechanical and dosimetric properties of the collimating of dynamic and/or step MLC system alter the generated intensity. This is mostly due to leaf transmission, leaf penumbra and geometry of leaves. The variations of output according to the multileaf opening during the irradiation need to be accounted for as well. These phenomena result in a fluence distribution that can be substantially different from the initial and calculative intensity modulation and therefore, should be taken into account by the treatment planning for accurate dose calculations delivered to the target volume in IMRT.

• 한국해양학회지:바다
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• 제5권2호
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• pp.95-104
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• 2000

울릉분지의 탄성파 단면에서 반사특성에 의해서 분류된 4개의 단위 퇴적층까지의 심도와 두께를 산출하고 분포도를 작성하였으며, 이를 중력 및 자기이상분포와 대비하여 울릉분지 심부구조의 특성을 해석하였다. 울릉분지 퇴적층은 중앙부에서 3,000-4,000 m이나 국지적으로 6,000m까지 두꺼워 지며, 북동쪽에서 남서쪽으로 두꺼운 지역의 분포가 넓어지는 양상을 보인다. 음향기반은 분지 중앙부에서 5,000 m, 남서단 및 서단에서 최대 7,500 m까지 지역적으로 깊어지며 전체적으로 남서-북동 방향의 긴 저지대를 이루면서 음향기반 상부 퇴적층들의 구조에 직접적인 영향을 주고 있다. 울릉분지 서쪽 및 남쪽 가장자리의 저중력분포는 두꺼운 퇴적층을 갖는 기반함몰대및 대륙-해양의 전이지각의 존재를 반영하며, 특히 기반함몰대는 탄성파 자료에 근거한 것과 같이 국지적이지 않고 북쪽의 한국대지 남단에서부터 울릉분지의 남단까지 주변부를 따라 연속적으로 발달하고 있다. 분지 중앙부에서 고중력이상분포는 북동단이 넓고 남서단쪽으로 좁아지는데, 이것은 고밀도 지각과 맨틀의 천부 존재를 지시한다. 울릉분지 중앙부의 북서부 및 남동부 가장자리를 따라 나타나는 한 쌍의 자기이상 선상분포는 탄성파 자료에서 밝혀진 분지내 기반암 저지대의 경계부와 대체적으로 일치한다. 이 자기선상 분포는 울릉분지가 해저확장하면서 형성된 대륙지각과 해양지각간 전이대의 열개된 지각틈새를 따라 관입 혹은 분출된 일련의 화성암체와 관련이있다. 탄성파 속도 분포가 울릉분지 중앙부에 해양지각이 존재할 가능성을 시사하는 것에 근거한다면 울릉분지내해양지각은 이 한 쌍의 자기선구조를 경계로 그 안쪽인 남서부에서 좁고 북동쪽으로 가면서 120 km까지 넓어지는 한정된 지역에만 분포하는 것으로 해석된다. 분지지각의 남쪽경계가 휘어져 있는 것은 필리핀판 등 해양지각판이 일본열도에 충돌할 때 받은 압축력의 영향으로 울릉분지 지괴가 변형 받았다는 것을 지시한다.