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Effect of Antibody Titer on Xenograft Survival in Pig-To-Dog Heterotopic Cardiac Xenotransplantation -Opening of Xenotrasplantation Era- (돼지\longrightarrow개 이소이종심장 이식모델에시 생존에 미치는 항체 역가의 영향 -이종이식시대의 개막-)

  • 이정렬;김희경;김지연;최대영;이재형;위현초;강희정;김영태;강병철
    • Journal of Chest Surgery
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    • v.37 no.5
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    • pp.391-400
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    • 2004
  • Xenotransplantation in discordant species results in immediate and irreversible hyperacute rejection due to natural antibodies, IgM. With this, antibody depletion is one option to reduce hyperacute rejection, we investigated the effect of PCPP (postcentrifugal plasmapheresis) on the depletion of natural antibodies and the effect of antibody titer on xenograft survival. Material and Method: Outbred swines (n=4) weighing 10∼20 kg were used as donors and mongrel dogs (n=4) weighing 25∼30 kg were used as recipients. Recipient canines underwent plasmapheresis (COBE TPE Laboratories, Lakewood. CO, USA). Pre-transplantation PCPP was peformed on day -2 and day 0. There were three groups (Group 0: no PCPP, Group 1: 1 pla sma-volume (PV) at day -2 and 2 PV at day 0, Group 2: 2 PV at day -2 and 2 PV at day 0). A swine heart was heterotopically transplanted into a recipient's abdominal infrarenal aorta and inferior vena cava. Mean percent depletion of total IgM and IgG in plasma of the recipients was calculated. Serum albumin, electrolyte, complement activity and coagulation factors were measured. Histopathologic examination of heart specimens was performed. Result: Mean percent depletion of IgM and IgG were 95.7$\pm$1.2%, 80.5$\pm$2.4% in the group 2 at the end of PCPP. The percent depletion of serum albumin concentration was decreased from 2.8 to 1.4 g/㎗ in the group 1 and 3.0 to 1.5 g/㎗ in the group 2. Complement hemolytic activity was decreased in group 1 and 2, but returned to normal level within 24 hours. Complement hemolytic activity was reduced to 10% of pre-PCPP level in group 2. Serum fibrinogen decreased to 20% or less and was recovered within 24 hours in group 2. Antithrombin III decreased but less than fibrinogen. PT and aPTT were sometimes but not always prolonged during plasmapheresis. After plasmapheresis, PT and aPTT were prolonged beyond the measurable level. D-dimer was not found during PCPP, but appeared and maintained from 10 minutes after trasplantation. Graft Survival time was 5 min in group 0, and it was 90$\pm$0 min in the group 2. Histopathologic changes were more typically characterized by edema, hemorrhages, thrombosis in all groups at the end of experiment. Conclusion: PCPP effectively removed immuoglobulins and reduced the titer of natural antibodies, as a result, significantly prololonged swine heart xenograft survival.

A Study on Developing Women's Vest Designs through the Application of Traditional Bojaki Technique (전통보자기 기법을 응용한 배자 디자인 개발에 관한 연구)

  • Park, Ji Won;Lee, Mi Seok;Kim, Chung Ho
    • Journal of the Korean Society of Costume
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    • v.67 no.3
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    • pp.133-148
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    • 2017
  • The purpose of this paper is to develop waist coat designs that can be worn in the present day by researching design techniques and traditional waist coat forms shown on Korean patchworks called 'Jokakbo', which was commonly used in our traditional culture. By selecting patchwork as the primary motive in particular. The Bojaki, also known as deungguhli or baeguhli(背巨里), gives off a natural and vibrant color sensation. A new design that could be practically worn with modern clothing was developed by sufficiently reflecting this color sensation as well as the aesthetic quality of its geometric shape. On the basis of theoretical consideration of traditional cloth and vest, total of 7 points were designed and produced. These works contain diverse application of the traditional Bojaki and vest. The design of the Bojaki grants rhythmic sense to the side where diverse colors and formative patterns shown on the cloth can be seen as monotonous. The entire side opening of the traditional waistcoat can cover multiple sizes. Depending on the movement, the front and back naturally spreads. The vest can also be worn in layers or used as a cotton quilt depending on the season. And after minimal design alterations, it will be possible to wear the vest with diverse modern clothing due to its straight line and form.

Factors of the Modernity of Belle Epoque and A study of phenomenon of Transitional Fashion (Belle Epoque의 의상 현대화의 요인과 과도기적 유행현상론 (1871~1914))

  • 김난공
    • Journal of the Korean Home Economics Association
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    • v.11 no.3
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    • pp.247-261
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    • 1973
  • In the past, what we call "fashion" was monopolized by only a small highly privileged group of individuals. To-day, we know that fashion field has become progressive democratization of taste not only in clothing, but in all expressions of contemporary living, from automobiles to refrigerators. So, we can find out how wonderful it is that our recent fashionable history was changed so fast. Whatever highly characteristic costume may be in the former, the way people dress was the reflection of their contemporary lives as well as their political status, economics, cultures, arts religions, so that a history of fashion is a history of life. Now, that categorical silhouettes make an exclusion across centuries of past history into the world of aesthetics, particularizes following ; symbolic voluminous toga of Roman authority, the religious but gorgeous Byzantine tunic, extravagant vertical bell-skirt of the Renaissance, the romantic Rococo style, the elegant crinoline and the bustle of the Cul de Paris of the nineteenth. It came true that women was intoxicated ostentations and elegances, since they had on ornamently costume which bear some relationship to the more formal Co-stesy, till the beginning of the twentieth. As Jonney Ironside said, "Nowadays, those exessive ornamentations and cumbersome design hardly belong to a civilization run by machines and in a hurry". These were once a sign of wealth and class ; at the beginning of the twentieth it was disappearing step by step. What is the reason\ulcorner At the end of the nineteenth, the emancipation of women, the movement of the Art Nouveau and the opening of the ready-made, have influenced on modern style, directly or indirectly. Finally, democratically popular costume was caused by fighting against the masculine prejudice excluded them from activities.hem from activities.

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The Characteristics of Behavior for Ventilation and Maintenance for Ventilation Equipments by Multi-family Housing Residents to Actualize Healthy Housing (건강주택 실현을 위한 공동주택 거주자의 환기행태 및 환기기기 관리 특성에 관한 연구)

  • Lee, Youn-Jae
    • Journal of the Korean housing association
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    • v.21 no.5
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    • pp.93-101
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    • 2010
  • The purpose of the study is to investigate the behaviors for ventilation to control indoor air quality and characteristics of the maintenance for ventilation equipments by multi-family housing residents. This study was conducted by the survey in the area of Seoul and Kyungki-do. The results of the study are as follows: the majority of respondents conduct ventilation once a day, below 20 minutes with opening some windows of the inside of the house. The time for ventilation is generally between 9-11 am. and 5-7 pm. Most of the wives are responsible for ventilation activities. In addition, so far building materials and furniture are not seriously considered as the source of indoor air pollution by the respondents. Their satisfaction for indoor air before and after ventilation is perceived as just the level of average. As the mechanical ventilation equipments such as a hood and an exhaust fan, half of the respondents are using them frequently. However, the ventilation equipments are not maintained well. Specially maintenance and checks for exhaust fans are hardly conducted. In conclusion, the frequency of ventilation is very limited and residents show passive attitudes about ventilation and maintenance of ventilation equipments. Therefore, for the actualization of healthy housing, educational programs and guidelines on the way of ventilation targeting the residents should be set up and a certain organization has to check periodically the performance of the ventilation equipments of each house in the multi-family housing complex.

Factors Involved in Promoting Seed Germination of Foeniculum vulgare (회향종자의 발아촉진에 관여하는 요인)

  • Eun Il, Lee;Chu Ho, Choi;Jong Ki, Lee;Seok Hyeon, Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.4
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    • pp.435-445
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    • 1997
  • The effects of temperature, prechilling, chemicals such as $GA_3$, IAA, kinetin and $KNO_3$ on the germinability of Foeniculum vulgare seed of medicinal plant were examined. In Foeniculum vulgare, the germination rate appeared to be around 50~60% or more in general, showing no differences in germination rate with different temperatures, promoting substances, physical or chemical treatments, and prechilling treatments. The observation of embryo under stereoscopic microscope for Foeniculum vulgare in Umbelliferae showed that seeds with or without embryo was almost the same in number. This result suggests that the lower rate of germination in this species is caused by embryolessness of seeds. The straight-shaped embryos as well as Y-shaped embryos were also observed. Foeniculum vulgare of medicinal plants in Umbelliferae were observed under scanning electron microscope, and did not show any opening problem near micropyle area. Final count should be made on 7th day of germination test.

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Ruptured Left Sinus of Valsalva Aneurysm into the Left Ventricle (좌심실로 파열된 좌발살바동 동맥류)

  • 임한중;이재원;송명근
    • Journal of Chest Surgery
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    • v.32 no.7
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    • pp.665-667
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    • 1999
  • We report a case of ruptured sinus of Valsalva aneurysm in 48-year-old male, presenting the symptom of congestive heart failure. Echocardiography showing a hairpin-like sac ex tended from the left coronary sinus to the left ventricle adjacent to the anterior mitral valve leaflet, suggested ruptured sinus of Valsalva aneurysm or aorto-left ventricular tunnel. Operative findings revealed that left sinus of Valsalva aneurysm had multiple openings faced left ventricle, 7 mm in diameter, 20 mm in length. The proximal opening was closed with bovine pericardium and distal openings were closed with spaghetti pledgeted reinforced sutures. The patient was discharged on the 14th postoperative day, and follow up for 8 months uneventfully. This case was reported for its rarity and to describe the techniques of surgical repair.

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Endoscopic Third Ventriculostomy for Adult Aqueduct Stenosis : Double Fenestration - A Case Report and Technical Note - (성인 수도관 폐쇄증에 대한 내시경적 제3뇌실 누공술 : 이중개창술 - 증례보고 및 수술수기 -)

  • Shim, Yong-Jin;Ha, Ho-Gyun;Jung, Ho;Kim, Yong-Seog;Park, Moon-Sun
    • Journal of Korean Neurosurgical Society
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    • v.29 no.8
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    • pp.1019-1023
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    • 2000
  • Objective : Endoscopic third ventriculostomy is gaining popularity as a minimally invasive surgical option for certain types of hydrocephalus as an alternative to shunting. The authors have tried to fenestrate down to the subdural space passing through the prepontine cistern to lessen or avoid the chance of redoing due to healing. Materials and Method : A 48-year-old male patient with several years of intractable headache was presented. Magnetic Resonance Image(MRI) of the brain revealed marked ventricular dilatation with stenotic cerebral aqueduct. A 2.3mm flexible steerable endoscope($Neuroview^{(R)}$) was introduced via precoronal route and accessed to the third ventricular floor. Using 3-French Fogarty balloon catheter, thin third ventricular floor and the arachnoid membrane of the prepontine cistern were fenestrated, so called "double fenestration". To confirm the fenestration, subdural compa-rtment of the left abducens nerve was identified during the procedure. Forceful pulsating flow through the orifice convinced the patency of the opening. Results : The patient was discharged on the third postoperative day without any postoperative complications. The postoperative follow-up MRI of the brain, at second and sixth months, clearly demonstrated the flow void through the third ventricular floor. Conclusions : Endoscopic third ventriculostomy was successfully performed on an adult hydrocephalus patient with aqueduct stenosis. The third ventricular floor and arachnoid membrane of the prepontine cistern were fenestrated to achieve double fenestration to minimize the chance for failure. The details of this procedure and results are described.

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Tegumental Ultrastructure of Adult Gynaecotyla squatarolae (Digenea: Microphallidae)

  • Lim, Do-Seon;Choi, Ki-Ju;Guk, Sang-Mee;Chai, Jong-Yil;Park, Il-Yong;Park, Yun-Kyu;Seo, Min
    • Parasites, Hosts and Diseases
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    • v.46 no.2
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    • pp.87-90
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    • 2008
  • Gynaecotyla squatarolae (Digenea: Microphallidae) adult flukes were recovered from experimental chicks at day 4-6 post-infection and their tegumental ultrastructure was observed with a scanning electron microscopy. They were pyriform in shape, and their anterior halves were concaved ventrally. The whole body surface was covered with tegumental spines, which were wide and 16-17 digitated between oral and ventral suckers. The density of spines and number of digits decreased posteriorly. The oral sucker was subterminal and the excretory pore was at the posterior end of the worm. Two ventral suckers were similar in appearance and protruded near midline of the worm. The genital atrium was dextral to the small ventral sucker. The dorsal surface was covered with tegumental spines, but the spines were sparser than on the ventral surface. On the middle portion of the dorsal surface, a small opening presumed to be the Laurer's canal was seen. From these findings, it has been confirmed that the adult G. squatarolae has unique characteristics in the surface ultrastructure.

Temporal and Spatial Variations of Sinking-particle Fluxes in the Northwestern Subtropical Pacific (북서태평양 아열대 해역에서 침강입자 플럭스의 시·공간 변동)

  • Kim, Hyung-Jeek;Hyeong, Ki-Seong;Yoo, Chan-Min;Jeon, Dong-Chull;Jeong, Jin-Hyun;Khim, Boo-Keun;Kim, Dong-Seon
    • Ocean and Polar Research
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    • v.33 no.spc3
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    • pp.385-395
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    • 2011
  • Time-series sediment traps were deployed at 1,000 m water depth of the northwestern subtropical Pacific from July 2009 to June 2010, with the aim of understanding temporal and spatial variations of sinking-particle fluxes. The opening and closing of the traps was synchronized at 18-day periods for 20 events. Total mass fluxes showed distinct seasonal variations with high values for the summer-fall seasons and relatively low values for winter-spring. This seasonal variation at two stations was characterized by a distinct difference in $CaCO_3$ fluxes between the two seasons. The enhanced $CaCO_3$ flux in the summer - fall seasons might be attributed to an increased planktonic foraminiferal flux. Total mass flux at FM10 station was nearly 50% higher than that at FM1 station. The difference in $CaCO_3$ fluxes between two stations contributed nearly 70% of the difference of total mass fluxes. The $CaCO_3$ flux was a major component controlling temporal and spatial variation of sinking - particle fluxes in the western subtropical Pacific Ocean.

Collapse of steel cantilever roof of tribune induced by snow loads

  • Altunisik, Ahmet C.;Ates, Sevket;Husem, Metin;Genc, Ali F.
    • Steel and Composite Structures
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    • v.23 no.3
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    • pp.273-283
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    • 2017
  • In this paper, it is aimed to present a detail investigation related to structural behavior of laterally unrestrained steel cantilever roof of tribune with slender cross section. The structure is located in Tutak town in $A{\breve{g}}r{{\i}}$ and collapsed on October 25, 2015 at eastern part of Turkey is considered as a case study. This mild sloped roof structure was built from a variable I beam, and supported on steel columns of 5.5 m height covering totally $240m^2$ closed area in plan. The roof of tribune collapsed completely without any indication during first snowfall after construction at midnight a winter day, fortunately before the opening hours. The meteorological records and observations of local persons are combined together to estimate the intensity of snow load in the region and it is compared with the code specified values. Also, the wide/thickness and height/thickness ratios for flange and web are evaluated according to the design codes. Three dimensional finite element model of the existing steel tribune roof is generated considering project drawings and site investigations using commercially available software ANSYS. The displacements, principal stresses and strains along to the cantilever length and column height are given as contour diagrams and graph format. In addition to site investigation, the numerical and analytical works conducted in this study indicate that the unequivocal reasons of the collapse are overloading action of snow load intensity, some mistakes made in the design of steel cantilever beams, insufficient strength and rigidity of the main structural elements, and construction workmanship errors.